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The map label for this gene is nspS

Identifier: 227080884

GI number: 227080884

Start: 711055

End: 712134

Strand: Reverse

Name: nspS

Synonym: VCM66_0662

Alternate gene names: 227080884

Gene position: 712134-711055 (Counterclockwise)

Preceding gene: 227080885

Following gene: 227080883

Centisome position: 24.62

GC content: 46.76

Gene sequence:

>1080_bases
GTGACCAATTTTTGTAACGAATGGGTATCGTATTCACAAATGATCAAACGCTTTCTGTCGCTGATGGTATTGAATACTGT
CTGTTATCAAGCCAGCGCTTTGGAACTGAATGTCTACCTTTGGGAAGATACGATTGCACCAAGCGTAGTTGAGGCATGGC
ATAAAAAAACCGGCGTCTCCGTTAATCTCTTCCATTTCGATAATGATGATGAACGCAGTTTATTAATGCTCAAAAGCGTA
CAACTGCCCTTTGACATCATGGTTTTGGATAACGTCTCCGCGTTCATTTTCTCAAGGCAAAATGTTTTTGAAGATCTGAC
CGCCCTTCCCAATCGTGCGAATAATGACCCTATGTGGCTACAAGCTTGTGGCACCCATGCGGTACCTTATTTCTGGGGCT
CAGTCGGCATTGCCTATCGCAAAAGCTTGTTTGATAAACCTCCAACGCAGTGGAGTGAGGTCGTCGATATCGCTCCGGCG
CATCGTGGACGAGTGGGTATGCTTAAAGACAGTGTCGAAACACTACTGCCTGCACTATACATGCTCAACGCCTCTCCCAT
TACCGATTCTATCGACACACTAAGGCAAGCGTATCGTCTCTTAGACGCCGCCAATCCCCATATCCTGACTTATGAGTATG
TATTGAGCTATGTACGCAGCCACCCACAAACCGACAATCTACATATGGCGGTCTCTTACAGTGGCGATCACTATTCACTG
AACCGCTTCTTCAATACTCAAGACTGGGACTTTAGCGTTCCAGAAGGCCGCCCTTATTTGTGGGTTGATTGTATGGCCGT
AAATAGCGTTTCTCCCAACACAGTGCAAGCCAAAGCTTTTTTGGATTTCTTGATGAAACCTGACATTGCCGCAATCAATG
CCGAATATATTCGAGCTGCAAGCCCGAACTACAAAGCACGCGCTTTGCTCCCTGTAGAGCACCGAGAAGATCTCTCGATT
TATCTCCCAGAGCAGCGATTGGCGGAGGGGATAATCGACAGCGAACTCTCGGCGAAAAACCTCAGTCTGCGTGCCAAAAT
CATCAGCAGTGTGACTTACCAATATGAAGCTAAACCATAG

Upstream 100 bases:

>100_bases
CTGATTATAATGCGACCAAATTATACATAGAGTTAGGTGATCCAGGCTGTCAGCGTAGCCAAAATATTGGCTTTTACGCT
ATTAGGTTATTTGTCGGATC

Downstream 100 bases:

>100_bases
AATTCTGTTACTGATTGCACCGGTGATCCTGCTCAGTGCAGCCGCATCAAGCTACATCATTTATACCAGTCAGAAAAATG
CGCTGCTAAAAAGAACCGAC

Product: putative spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: Norspermidine-binding protein

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTNFCNEWVSYSQMIKRFLSLMVLNTVCYQASALELNVYLWEDTIAPSVVEAWHKKTGVSVNLFHFDNDDERSLLMLKSV
QLPFDIMVLDNVSAFIFSRQNVFEDLTALPNRANNDPMWLQACGTHAVPYFWGSVGIAYRKSLFDKPPTQWSEVVDIAPA
HRGRVGMLKDSVETLLPALYMLNASPITDSIDTLRQAYRLLDAANPHILTYEYVLSYVRSHPQTDNLHMAVSYSGDHYSL
NRFFNTQDWDFSVPEGRPYLWVDCMAVNSVSPNTVQAKAFLDFLMKPDIAAINAEYIRAASPNYKARALLPVEHREDLSI
YLPEQRLAEGIIDSELSAKNLSLRAKIISSVTYQYEAKP

Sequences:

>Translated_359_residues
MTNFCNEWVSYSQMIKRFLSLMVLNTVCYQASALELNVYLWEDTIAPSVVEAWHKKTGVSVNLFHFDNDDERSLLMLKSV
QLPFDIMVLDNVSAFIFSRQNVFEDLTALPNRANNDPMWLQACGTHAVPYFWGSVGIAYRKSLFDKPPTQWSEVVDIAPA
HRGRVGMLKDSVETLLPALYMLNASPITDSIDTLRQAYRLLDAANPHILTYEYVLSYVRSHPQTDNLHMAVSYSGDHYSL
NRFFNTQDWDFSVPEGRPYLWVDCMAVNSVSPNTVQAKAFLDFLMKPDIAAINAEYIRAASPNYKARALLPVEHREDLSI
YLPEQRLAEGIIDSELSAKNLSLRAKIISSVTYQYEAKP
>Mature_358_residues
TNFCNEWVSYSQMIKRFLSLMVLNTVCYQASALELNVYLWEDTIAPSVVEAWHKKTGVSVNLFHFDNDDERSLLMLKSVQ
LPFDIMVLDNVSAFIFSRQNVFEDLTALPNRANNDPMWLQACGTHAVPYFWGSVGIAYRKSLFDKPPTQWSEVVDIAPAH
RGRVGMLKDSVETLLPALYMLNASPITDSIDTLRQAYRLLDAANPHILTYEYVLSYVRSHPQTDNLHMAVSYSGDHYSLN
RFFNTQDWDFSVPEGRPYLWVDCMAVNSVSPNTVQAKAFLDFLMKPDIAAINAEYIRAASPNYKARALLPVEHREDLSIY
LPEQRLAEGIIDSELSAKNLSLRAKIISSVTYQYEAKP

Specific function: Acts as a sensor of norspermidine and enhances biofilm formation. When complexed to norspermidine, could interact with the periplasmic portion of mbaA to regulate its enzymatic activity

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family

Homologues:

Organism=Escherichia coli, GI1787078, Length=330, Percent_Identity=27.8787878787879, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787367, Length=313, Percent_Identity=22.3642172523962, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NSPS_VIBCH (Q9KU25)

Other databases:

- EMBL:   AE003852
- PIR:   B82291
- RefSeq:   NP_230353.1
- ProteinModelPortal:   Q9KU25
- SMR:   Q9KU25
- GeneID:   2615708
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0704
- TIGR:   VC_0704
- HOGENOM:   HBG739658
- OMA:   LCASASR
- ProtClustDB:   CLSK2392352
- InterPro:   IPR006059
- InterPro:   IPR001188
- PRINTS:   PR00909

Pfam domain/function: PF01547 SBP_bac_1

EC number: NA

Molecular weight: Translated: 40899; Mature: 40768

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNFCNEWVSYSQMIKRFLSLMVLNTVCYQASALELNVYLWEDTIAPSVVEAWHKKTGVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCE
VNLFHFDNDDERSLLMLKSVQLPFDIMVLDNVSAFIFSRQNVFEDLTALPNRANNDPMWL
EEEEEECCCCCCCEEEEEECCCCEEEEEECCCCEEHHHHHHHHHHHHHCCCCCCCCCEEE
QACGTHAVPYFWGSVGIAYRKSLFDKPPTQWSEVVDIAPAHRGRVGMLKDSVETLLPALY
EECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MLNASPITDSIDTLRQAYRLLDAANPHILTYEYVLSYVRSHPQTDNLHMAVSYSGDHYSL
HHCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCEEEEEEECCCEEEE
NRFFNTQDWDFSVPEGRPYLWVDCMAVNSVSPNTVQAKAFLDFLMKPDIAAINAEYIRAA
EECCCCCCCCEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCHHEECHHHHCCC
SPNYKARALLPVEHREDLSIYLPEQRLAEGIIDSELSAKNLSLRAKIISSVTYQYEAKP
CCCCCEEEEEECCCCCCCEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure 
TNFCNEWVSYSQMIKRFLSLMVLNTVCYQASALELNVYLWEDTIAPSVVEAWHKKTGVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCE
VNLFHFDNDDERSLLMLKSVQLPFDIMVLDNVSAFIFSRQNVFEDLTALPNRANNDPMWL
EEEEEECCCCCCCEEEEEECCCCEEEEEECCCCEEHHHHHHHHHHHHHCCCCCCCCCEEE
QACGTHAVPYFWGSVGIAYRKSLFDKPPTQWSEVVDIAPAHRGRVGMLKDSVETLLPALY
EECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MLNASPITDSIDTLRQAYRLLDAANPHILTYEYVLSYVRSHPQTDNLHMAVSYSGDHYSL
HHCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCEEEEEEECCCEEEE
NRFFNTQDWDFSVPEGRPYLWVDCMAVNSVSPNTVQAKAFLDFLMKPDIAAINAEYIRAA
EECCCCCCCCEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCHHEECHHHHCCC
SPNYKARALLPVEHREDLSIYLPEQRLAEGIIDSELSAKNLSLRAKIISSVTYQYEAKP
CCCCCEEEEEECCCCCCCEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301