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The map label for this gene is yhbU [H]

Identifier: 227080833

GI number: 227080833

Start: 653682

End: 654695

Strand: Reverse

Name: yhbU [H]

Synonym: VCM66_0610

Alternate gene names: 227080833

Gene position: 654695-653682 (Counterclockwise)

Preceding gene: 227080834

Following gene: 227080832

Centisome position: 22.63

GC content: 50.1

Gene sequence:

>1014_bases
ATGGAACTTCTCTGTCCGGCCGGAAATTTGCCGGCCCTGAAAACAGCGATTGATTGTGGTGCCGATGCGGTTTATATCGG
TTTCAAAGATGATACCAACGCTCGCCACTTTGCCGGCCTTAATTTTAGCGGCAAAAAGCTCGAAAAAGCGGTGGATTACG
TGCACGACCACAATAAAAAAATTCATATTGCACTCAACACCTTCGCCCATCCTAATGGTTTCGAGCGCTGGACTAACGCG
GTAGATCAAGCGGCCGATCTCGGGGTTGATGCCTTGATCATTGCCGATATTGCCGTGCTGGAGTATGCGGCGCGCCACTA
CCCACACCTTGAACTGCACCTTTCCGTGCAAGCATCAGCCACCAACGTGAAAGCCGTTGAATTTTATCATCAGAATTTTA
ACGTCAAACGTGTCGTTCTGCCGCGCGTGCTTTCCATTCATCAGGTGAAGCAGCTTTCGCGTAATATTCCACAAGGTGTC
GAGCTGGAAGTGTTCGCTTTTGGCAGCTTGTGCATCATGTCGGAAGGCCGCTGTTATCTCTCCTCTTACATGACGGGTGA
GTCGCCTAATACGGTTGGAGCTTGCTCTCCCGCCAAATATGTACGCTGGCAAGAGACAGAATCAGGGCTTGAATCTCGCT
TGAACGAAATTTTGATTGATAAGTATGCGGCGGGGGAAAATGCAGGTTATCCAACCCTGTGTAAAGGGCGCTTCATCACG
GATATTGAAGGTGAACGAAAGTGCTATCACGCTCTTGAAGAGCCAACCAGTTTAAATACCTTGTCACTGCTGCCAGAGCT
GTTTGCCGCCAATGTGGCTTCAGTAAAAATTGAGGGGCGTCAACGAAGCCCTGCGTATGTCGAACAAGTCACCCGCACTT
GGCGTGCCGCGATTGATCGCTACTTAGCCAATCCTGCGCAATACCATGTCGAACCCAAATGGAATGCGGCACTGGCCAAT
GTCTCAGAAGGTACCCAAACCACACTTGGTGCCTATCACCGTCAATGGCAATAA

Upstream 100 bases:

>100_bases
ATTTTACTCTGATTGGTGGAAACCTGCCTTACATCAAATCGGGGAGACTCAATCCTTCCTAAAATGCGTTCACTATTCCC
TGAATTCAGAGCCTTACGCT

Downstream 100 bases:

>100_bases
GGGCAACGATCATGAAATATGCTTTAGGTCCACTACTCTATTTCTGGCCAAAACAGGACGTTGAAAGTTTCTATACTCAA
GCCGCCGCCAGCATGGCGGA

Product: putative protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MELLCPAGNLPALKTAIDCGADAVYIGFKDDTNARHFAGLNFSGKKLEKAVDYVHDHNKKIHIALNTFAHPNGFERWTNA
VDQAADLGVDALIIADIAVLEYAARHYPHLELHLSVQASATNVKAVEFYHQNFNVKRVVLPRVLSIHQVKQLSRNIPQGV
ELEVFAFGSLCIMSEGRCYLSSYMTGESPNTVGACSPAKYVRWQETESGLESRLNEILIDKYAAGENAGYPTLCKGRFIT
DIEGERKCYHALEEPTSLNTLSLLPELFAANVASVKIEGRQRSPAYVEQVTRTWRAAIDRYLANPAQYHVEPKWNAALAN
VSEGTQTTLGAYHRQWQ

Sequences:

>Translated_337_residues
MELLCPAGNLPALKTAIDCGADAVYIGFKDDTNARHFAGLNFSGKKLEKAVDYVHDHNKKIHIALNTFAHPNGFERWTNA
VDQAADLGVDALIIADIAVLEYAARHYPHLELHLSVQASATNVKAVEFYHQNFNVKRVVLPRVLSIHQVKQLSRNIPQGV
ELEVFAFGSLCIMSEGRCYLSSYMTGESPNTVGACSPAKYVRWQETESGLESRLNEILIDKYAAGENAGYPTLCKGRFIT
DIEGERKCYHALEEPTSLNTLSLLPELFAANVASVKIEGRQRSPAYVEQVTRTWRAAIDRYLANPAQYHVEPKWNAALAN
VSEGTQTTLGAYHRQWQ
>Mature_337_residues
MELLCPAGNLPALKTAIDCGADAVYIGFKDDTNARHFAGLNFSGKKLEKAVDYVHDHNKKIHIALNTFAHPNGFERWTNA
VDQAADLGVDALIIADIAVLEYAARHYPHLELHLSVQASATNVKAVEFYHQNFNVKRVVLPRVLSIHQVKQLSRNIPQGV
ELEVFAFGSLCIMSEGRCYLSSYMTGESPNTVGACSPAKYVRWQETESGLESRLNEILIDKYAAGENAGYPTLCKGRFIT
DIEGERKCYHALEEPTSLNTLSLLPELFAANVASVKIEGRQRSPAYVEQVTRTWRAAIDRYLANPAQYHVEPKWNAALAN
VSEGTQTTLGAYHRQWQ

Specific function: Unknown

COG id: COG0826

COG function: function code O; Collagenase and related proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U32 family [H]

Homologues:

Organism=Escherichia coli, GI1789548, Length=337, Percent_Identity=73.5905044510386, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1788397, Length=319, Percent_Identity=29.7805642633229, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87081910, Length=301, Percent_Identity=31.5614617940199, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001539 [H]

Pfam domain/function: PF01136 Peptidase_U32 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 37490; Mature: 37490

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS01276 PEPTIDASE_U32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLCPAGNLPALKTAIDCGADAVYIGFKDDTNARHFAGLNFSGKKLEKAVDYVHDHNKK
CCEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHCCCCE
IHIALNTFAHPNGFERWTNAVDQAADLGVDALIIADIAVLEYAARHYPHLELHLSVQASA
EEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
TNVKAVEFYHQNFNVKRVVLPRVLSIHQVKQLSRNIPQGVELEVFAFGSLCIMSEGRCYL
CCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEECCCEEE
SSYMTGESPNTVGACSPAKYVRWQETESGLESRLNEILIDKYAAGENAGYPTLCKGRFIT
HHHCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEE
DIEGERKCYHALEEPTSLNTLSLLPELFAANVASVKIEGRQRSPAYVEQVTRTWRAAIDR
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
YLANPAQYHVEPKWNAALANVSEGTQTTLGAYHRQWQ
HHCCCHHEEECCCCCHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MELLCPAGNLPALKTAIDCGADAVYIGFKDDTNARHFAGLNFSGKKLEKAVDYVHDHNKK
CCEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHCCCCE
IHIALNTFAHPNGFERWTNAVDQAADLGVDALIIADIAVLEYAARHYPHLELHLSVQASA
EEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
TNVKAVEFYHQNFNVKRVVLPRVLSIHQVKQLSRNIPQGVELEVFAFGSLCIMSEGRCYL
CCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEECCCEEE
SSYMTGESPNTVGACSPAKYVRWQETESGLESRLNEILIDKYAAGENAGYPTLCKGRFIT
HHHCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEE
DIEGERKCYHALEEPTSLNTLSLLPELFAANVASVKIEGRQRSPAYVEQVTRTWRAAIDR
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
YLANPAQYHVEPKWNAALANVSEGTQTTLGAYHRQWQ
HHCCCHHEEECCCCCHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]