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The map label for this gene is trmB [H]

Identifier: 227080663

GI number: 227080663

Start: 469254

End: 469973

Strand: Direct

Name: trmB [H]

Synonym: VCM66_0438

Alternate gene names: 227080663

Gene position: 469254-469973 (Clockwise)

Preceding gene: 227080658

Following gene: 227080664

Centisome position: 16.22

GC content: 51.94

Gene sequence:

>720_bases
ATGAGCGAAGTCATCACCCAGGAATATACCGAAGATGGCAAAGTGCTGCGTCGTATTCGTAGCTTTGTTCGTCGTGAAGG
TCGTTTGACCAAAGGCCAGGAAGCCGCAATGAAAGAGTGCTGGCCAACCATGGGGATTGATTACCAGCCAGAGCTGTTGG
ATTGGCAACAGGTGTTTGGTAACGATAATCCGGTTGTGCTGGAGATTGGTTTTGGCATGGGCGCGTCCTTGGTCGAAATG
GCGAAAAACGCACCTGAGAAAAACTTCCTCGGTATTGAAGTGCACAGCCCAGGTGTCGGTGCTTGTTTGGCTTCGGCGCG
TGAAGCGGGTGTGACTAACTTGCGTGTGATGTGCCATGACGCGGTGGAAGTGTTTGCGCACATGATTCCAGATAACAGCC
TGCACACTTTGCAATTATTCTTCCCAGATCCTTGGCACAAGAAACGCCACCACAAGCGCCGTATCGTGCAGCTTGAATTT
GCTGAAATGGTGCGCCAAAAGCTCATGATTGGCAGCGGTGTATTCCATATGGCAACCGACTGGGAAAACTATGCAGAACA
CATGGTTGAAGTGATGAATCAAGCCCCCGGTTTTGCTAACTTGGCAACCGATGGCGATTACGTTCCGCGCCCTGATGAGC
GCCCACTGACCAAGTTTGAGCAGCGTGGTCACCGTTTAGGCCACGGAGTGTGGGACATCAAGTACCAGCGCACTGCGTAA

Upstream 100 bases:

>100_bases
GTTTGAGGGCAGGATGGTAAACGGTCAGTAATAACACTTGCACCGTTGACGATTCTTTGGATAATTCCCGCTCTGATTTA
GTAATGTGCAGGCAATAGAA

Downstream 100 bases:

>100_bases
AGAGAGAATTTTGCTTAAGCCAACATTTGATGTTGGCTTTTTTGACCCTAATCCTCAAGCTATTGAAAAGCTGAAGTAGC
TTGAGACAAACTAATAATAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFGNDNPVVLEIGFGMGASLVEM
AKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHDAVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEF
AEMVRQKLMIGSGVFHMATDWENYAEHMVEVMNQAPGFANLATDGDYVPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA

Sequences:

>Translated_239_residues
MSEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFGNDNPVVLEIGFGMGASLVEM
AKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHDAVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEF
AEMVRQKLMIGSGVFHMATDWENYAEHMVEVMNQAPGFANLATDGDYVPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA
>Mature_238_residues
SEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFGNDNPVVLEIGFGMGASLVEMA
KNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHDAVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEFA
EMVRQKLMIGSGVFHMATDWENYAEHMVEVMNQAPGFANLATDGDYVPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=237, Percent_Identity=67.9324894514768, Blast_Score=349, Evalue=8e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 27316; Mature: 27184

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHC
NDNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHD
CCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHH
AVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEFAEMVRQKLMIGSGVFHMATD
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
WENYAEHMVEVMNQAPGFANLATDGDYVPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHCCHHHCCCEEEEEEECCC
>Mature Secondary Structure 
SEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHC
NDNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHD
CCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHH
AVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEFAEMVRQKLMIGSGVFHMATD
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
WENYAEHMVEVMNQAPGFANLATDGDYVPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHCCHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA