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The map label for this gene is djlA

Identifier: 227080658

GI number: 227080658

Start: 462148

End: 463002

Strand: Direct

Name: djlA

Synonym: VCM66_0432

Alternate gene names: 227080658

Gene position: 462148-463002 (Clockwise)

Preceding gene: 227080651

Following gene: 227080663

Centisome position: 15.98

GC content: 50.29

Gene sequence:

>855_bases
ATGCATATTTTTGGCAAAATTCTTGGCGCTTTTTTTGGCTTCCTATTTGGTGGCCCCTTTGGCGCAATCTTTGGGATCTT
CCTTGGCCACCAGTTTGATAAGGCTCGTCGTTTAAATCAGGCTGGCTTTCAAAGTGGTACCTTTGGTGCAGGCCCTAGCC
AAGCTGAGCGCCAAGAGGAGTTTTTTAAATCCGCATTTTCGGTGATGGGGCATGTAGCAAAAGCCAAGGGTCAAGTCACC
AAAGAAGAGATCCAACTCGCGACCATCATGATGGATCGCATGAACCTGACCCTAGAGCAAAAACGTGCTGCACAAGATGC
GTTTCGTGACGGGAAAGAAAGCGATTTTCCTCTAGAGCAAGTGTTAGAGCGCGTGAAAATCGCCACGGGCGGGCGTTTTG
ACCTGCTACAGTTTTTCCTTGAGCTGCAAGTCTCTTCCGCGTTTGCTGATGGGGATGTGCACCCGAGTGAGCGTCAAGTA
CTGCATCGTATTGCGCGTGGGCTTGGTTTTTCTTCCGAGCAGTTAGAGCGCCGTTTACGCATGCAGGAAGCGGCGTTTCG
ATTTCAACAAGGTGGTGGCTTTGGTGGTTCGCAGCAGCAATCCCATAGTGGGCAGCAATGGCAGCAGCCTTCATCACGCC
ATCAATTGGCCGATGCCTACGAAGTGCTTGGGGTGAGCGAAAGTGCGAGTGCACAAGAGGTGAAGCGAGCTTATCGTAAG
CTGATGAATGAGCACCACCCAGATAAGCTGATGGCGAAAGGTTTACCGCCAGAGATGATGAACGTCGCGAAAGAAAAATC
TCAGCAAATCCAACACGCTTATGAGCTGATCCGAAAGGAGAAAGGCATTAAGTAA

Upstream 100 bases:

>100_bases
GAGCCTGAAATATAAACGGTCCTTATCATAAAGGAAATTGACGCTTGCGCCACTATCAGACCGCTTCACTCCAGAAAAAT
TCATAGATTGAGAGCACATA

Downstream 100 bases:

>100_bases
GCTCATCGTCATTATGCGCTTAGCTTTTCTAAGGCAAACTTAACCGTTTGCCTTTCTTTTGCAGTCAAAAGAGCAATTAG
AATGGGGCGGTTTTCGATAG

Product: Dna-J like membrane chaperone protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MHIFGKILGAFFGFLFGGPFGAIFGIFLGHQFDKARRLNQAGFQSGTFGAGPSQAERQEEFFKSAFSVMGHVAKAKGQVT
KEEIQLATIMMDRMNLTLEQKRAAQDAFRDGKESDFPLEQVLERVKIATGGRFDLLQFFLELQVSSAFADGDVHPSERQV
LHRIARGLGFSSEQLERRLRMQEAAFRFQQGGGFGGSQQQSHSGQQWQQPSSRHQLADAYEVLGVSESASAQEVKRAYRK
LMNEHHPDKLMAKGLPPEMMNVAKEKSQQIQHAYELIRKEKGIK

Sequences:

>Translated_284_residues
MHIFGKILGAFFGFLFGGPFGAIFGIFLGHQFDKARRLNQAGFQSGTFGAGPSQAERQEEFFKSAFSVMGHVAKAKGQVT
KEEIQLATIMMDRMNLTLEQKRAAQDAFRDGKESDFPLEQVLERVKIATGGRFDLLQFFLELQVSSAFADGDVHPSERQV
LHRIARGLGFSSEQLERRLRMQEAAFRFQQGGGFGGSQQQSHSGQQWQQPSSRHQLADAYEVLGVSESASAQEVKRAYRK
LMNEHHPDKLMAKGLPPEMMNVAKEKSQQIQHAYELIRKEKGIK
>Mature_284_residues
MHIFGKILGAFFGFLFGGPFGAIFGIFLGHQFDKARRLNQAGFQSGTFGAGPSQAERQEEFFKSAFSVMGHVAKAKGQVT
KEEIQLATIMMDRMNLTLEQKRAAQDAFRDGKESDFPLEQVLERVKIATGGRFDLLQFFLELQVSSAFADGDVHPSERQV
LHRIARGLGFSSEQLERRLRMQEAAFRFQQGGGFGGSQQQSHSGQQWQQPSSRHQLADAYEVLGVSESASAQEVKRAYRK
LMNEHHPDKLMAKGLPPEMMNVAKEKSQQIQHAYELIRKEKGIK

Specific function: Regulatory dnaK co-chaperone. Direct interaction between dnaK and djlA is needed for the induction of the wcaABCDE operon, involved in the synthesis of a colanic acid polysaccharide capsule, possibly through activation of the rcsB/rcsC phosphotransfer sig

COG id: COG1076

COG function: function code O; DnaJ-domain-containing proteins 1

Gene ontology:

Cell location: Cell inner membrane; Single-pass type III membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain

Homologues:

Organism=Escherichia coli, GI1786241, Length=285, Percent_Identity=47.719298245614, Blast_Score=251, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6324828, Length=66, Percent_Identity=51.5151515151515, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DJLA_VIBCH (Q9KUR8)

Other databases:

- EMBL:   AE003852
- PIR:   G82319
- RefSeq:   NP_230101.1
- ProteinModelPortal:   Q9KUR8
- SMR:   Q9KUR8
- GeneID:   2615779
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0447
- TIGR:   VC_0447
- HOGENOM:   HBG508481
- OMA:   LRACWRM
- ProtClustDB:   PRK09430
- HAMAP:   MF_01153
- InterPro:   IPR001623
- InterPro:   IPR007791
- InterPro:   IPR015609
- InterPro:   IPR003095
- Gene3D:   G3DSA:1.10.287.110
- PANTHER:   PTHR11821
- PRINTS:   PR00625
- SMART:   SM00271

Pfam domain/function: PF00226 DnaJ; PF05099 TerB; SSF46565 DnaJ_N

EC number: NA

Molecular weight: Translated: 31896; Mature: 31896

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00636 DNAJ_1; PS50076 DNAJ_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x241e7d5c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MHIFGKILGAFFGFLFGGPFGAIFGIFLGHQFDKARRLNQAGFQSGTFGAGPSQAERQEE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
FFKSAFSVMGHVAKAKGQVTKEEIQLATIMMDRMNLTLEQKRAAQDAFRDGKESDFPLEQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VLERVKIATGGRFDLLQFFLELQVSSAFADGDVHPSERQVLHRIARGLGFSSEQLERRLR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
MQEAAFRFQQGGGFGGSQQQSHSGQQWQQPSSRHQLADAYEVLGVSESASAQEVKRAYRK
HHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LMNEHHPDKLMAKGLPPEMMNVAKEKSQQIQHAYELIRKEKGIK
HHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301