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The map label for this gene is folA [H]

Identifier: 227080651

GI number: 227080651

Start: 454525

End: 455022

Strand: Direct

Name: folA [H]

Synonym: VCM66_0425

Alternate gene names: 227080651

Gene position: 454525-455022 (Clockwise)

Preceding gene: 227080650

Following gene: 227080658

Centisome position: 15.71

GC content: 50.2

Gene sequence:

>498_bases
ATGATGATCAGTATGATTGCCGCGATGGCAGACCAGCGCATTATTGGTAAAGATAACCAGATGCCTTGGCATCTACCGGC
TGATTTCGCGTGGTTCAAACGCTGTACATTAGGTAAGCCTGTAGTGATGGGGCGTAAAACCTACCAATCGATCGGACGTC
CTTTACCGGGGCGGCACAATATTGTGATCTCCCGTGATGCGAGTTTGCAGATTGAGGGGGTGGATGTGGTGACCTCCATT
GAGGCGGCGCTGGCGAAAGCGGGTGAGGTCGACGAGGTGATGATCATCGGAGGTGGTAGCTTGTATGCGGCCTGTTTGCC
GATGGCCCATAAACTGTATATTACCGAGATCCATGCAAAATTGGATGGTGATACTCAGTTTCCTGAATGGGGGAGTGATT
GGCTAGAGCGCTCTCGTGAGCATTATCCTGCCGATGAAAAAAATGCCTACGGGATGGATTTTGTGATTTTTGAACGCCAA
TATCCCCTTCCTACTTGA

Upstream 100 bases:

>100_bases
CCTTAAAGCCATGTTTATCATTGCGGGTTTGGCGGTTGGCCTTGCTGTACCTGGACTGTTATTCTACCGCCGCAAACCGA
TTATTTAAGGTAAGAGAAAA

Downstream 100 bases:

>100_bases
TGTGCAGCAGTGTTGGCTGCGCAAATTGGTTCTAGACACATTTGTCACTTATTTTGCCGCCTACCTGCAACTCCAAGTCG
TTTGGGGATCGTCATTGCAG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MMISMIAAMADQRIIGKDNQMPWHLPADFAWFKRCTLGKPVVMGRKTYQSIGRPLPGRHNIVISRDASLQIEGVDVVTSI
EAALAKAGEVDEVMIIGGGSLYAACLPMAHKLYITEIHAKLDGDTQFPEWGSDWLERSREHYPADEKNAYGMDFVIFERQ
YPLPT

Sequences:

>Translated_165_residues
MMISMIAAMADQRIIGKDNQMPWHLPADFAWFKRCTLGKPVVMGRKTYQSIGRPLPGRHNIVISRDASLQIEGVDVVTSI
EAALAKAGEVDEVMIIGGGSLYAACLPMAHKLYITEIHAKLDGDTQFPEWGSDWLERSREHYPADEKNAYGMDFVIFERQ
YPLPT
>Mature_165_residues
MMISMIAAMADQRIIGKDNQMPWHLPADFAWFKRCTLGKPVVMGRKTYQSIGRPLPGRHNIVISRDASLQIEGVDVVTSI
EAALAKAGEVDEVMIIGGGSLYAACLPMAHKLYITEIHAKLDGDTQFPEWGSDWLERSREHYPADEKNAYGMDFVIFERQ
YPLPT

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=147, Percent_Identity=36.0544217687075, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1786233, Length=160, Percent_Identity=51.25, Blast_Score=165, Evalue=1e-42,
Organism=Drosophila melanogaster, GI116008026, Length=182, Percent_Identity=31.8681318681319, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647458, Length=182, Percent_Identity=31.8681318681319, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18525; Mature: 18525

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMISMIAAMADQRIIGKDNQMPWHLPADFAWFKRCTLGKPVVMGRKTYQSIGRPLPGRHN
CHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCHHHHHHCCCCCCCCC
IVISRDASLQIEGVDVVTSIEAALAKAGEVDEVMIIGGGSLYAACLPMAHKLYITEIHAK
EEEECCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHEEEEEEEEE
LDGDTQFPEWGSDWLERSREHYPADEKNAYGMDFVIFERQYPLPT
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MMISMIAAMADQRIIGKDNQMPWHLPADFAWFKRCTLGKPVVMGRKTYQSIGRPLPGRHN
CHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCHHHHHHCCCCCCCCC
IVISRDASLQIEGVDVVTSIEAALAKAGEVDEVMIIGGGSLYAACLPMAHKLYITEIHAK
EEEECCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHEEEEEEEEE
LDGDTQFPEWGSDWLERSREHYPADEKNAYGMDFVIFERQYPLPT
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]