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The map label for this gene is cafA [H]

Identifier: 227080630

GI number: 227080630

Start: 429933

End: 431402

Strand: Direct

Name: cafA [H]

Synonym: VCM66_0404

Alternate gene names: 227080630

Gene position: 429933-431402 (Clockwise)

Preceding gene: 227080629

Following gene: 227080631

Centisome position: 14.86

GC content: 49.39

Gene sequence:

>1470_bases
ATGAGTGCAGAGTTGCTGTTAAACGTCACCCCGAGTGAAACTCGGGTGGCGATGATTGAAGGGGGAATCCTTCAGGAAAT
CCATATTGAACGTGAAGCCAAACGCGGCATTGTTGGCAATATTTACAAAGGCAAAGTCAGCCGGGTATTACCGGGCATGC
AAGCTGCTTTTGTGGATATTGGACTCGACAAGGCCGCTTTTTTACATGCCTCGGATATTGTGCCCCACACCGAGTGTGTT
GCCGAAAGTGAGAAGCAGCAGTTCCAAGTGCGCGATATTTCCGAGCTGGTGCGCCAAGGGCAAGATATTGTGGTGCAAGT
GGTCAAAGACCCGCTTGGTACTAAGGGTGCTCGCTTAACTACCGATATTACGCTCCCTTCTCGTTATCTGGTGTTTATGC
CGGGGGCAAGCCATGTTGGCGTATCACAACGCATCGAAAGCGAAGCGGAGCGCGAGCGCCTAAAGAAAATCGTTTCTCAT
TATTGCGATGAGCATGGCGGGTTCATTATCCGAACCGCAGCAGAAGGGGCAAACGAAAAAGAGTTGACTCAAGATGCGGC
GTTTCTTAAGCGTTTGTGGTCAAAAGTGATTGAGCGGCGCAGCAAATACAAGAACCGCTCTATGCTGTATGGCGAGCTTG
GTCTGGCGCAGCGCATTTTGCGTGACTTTGTCGGCACCGAACTGACCAAAATTCTCATCGATTCACGACAAGAGTTCGAA
AACCTCAAAGAGTTTACCTCCGAATATGTCCCTGAGCTAACGGCTAAATTAGAGCTGTACCACGGCGATAAGCCCATTTT
TGACATGTACGATACGGAAAATGAGATTCAGCGCTCTCTCGAACGCAAAGTGGAGCTCAAATCGGGGGGGTATCTGATCA
TCGATCAGACCGAAGCCATGACCACGATTGACATCAATACGGGCGCGTTTGTTGGCCGCCGTAATCTCGAAGAGACCATT
TTCAACACCAATATTGAAGCGACTCAAGCCATTGCTCGTCAGCTGCGTCTGCGCAATCTGGGTGGGATTATCATTATTGA
CTTTATCGATATGGCGTCGGATGAGCACCGTAAGCGAGTGCTCACCTCTTTAGAGACGGCGTTGATGAAAGATCGGGTCA
AAACCAACATCAACGGCTTTACTCAGCTTGGGTTGGTGGAGATGACGCGTAAAAGAACCCGAGAAAGCATTGAACATGTG
CTCTGTTCGACTTGTCCAACGTGTGAAGGGCGAGGCAGCGTGAAAACGGTGGAAACGGTGTGCTTTGAGATTTTGCGCGA
AATTACGCGCGTGAATCGAGCCTACGATGCGGACAAATTCGTGGTTTATGCTTCACCGGCAGTGGCCGATACTTTGCAAG
GTGAAGAGTCACATGCCTTGGCGGAGTTGGAAGTGTTCATTGGTAAAGAAGTGCGAATTCAAGCAGAACCACTCTACAAC
CAAGAGCAATTTGATGTTGTGATGATGTAA

Upstream 100 bases:

>100_bases
AGGATTGAAGGCAGCTACCACGCGGTAGTCGGATTGCCACTCTACGAGACTGACCAGCTTCTGCACAAATTCTAATCATT
TTAATCATTGAGGTGCGCTC

Downstream 100 bases:

>100_bases
ATAGGGCATCGGGTGATTTCGACAGTAACCCGCCTCGGGCGGATCCTTATGTGGCTGCTGGTCAGCCTGCTGATTGTGCT
GGCTTTAGCCGTCACAGCCC

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSAELLLNVTPSETRVAMIEGGILQEIHIEREAKRGIVGNIYKGKVSRVLPGMQAAFVDIGLDKAAFLHASDIVPHTECV
AESEKQQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESEAERERLKKIVSH
YCDEHGGFIIRTAAEGANEKELTQDAAFLKRLWSKVIERRSKYKNRSMLYGELGLAQRILRDFVGTELTKILIDSRQEFE
NLKEFTSEYVPELTAKLELYHGDKPIFDMYDTENEIQRSLERKVELKSGGYLIIDQTEAMTTIDINTGAFVGRRNLEETI
FNTNIEATQAIARQLRLRNLGGIIIIDFIDMASDEHRKRVLTSLETALMKDRVKTNINGFTQLGLVEMTRKRTRESIEHV
LCSTCPTCEGRGSVKTVETVCFEILREITRVNRAYDADKFVVYASPAVADTLQGEESHALAELEVFIGKEVRIQAEPLYN
QEQFDVVMM

Sequences:

>Translated_489_residues
MSAELLLNVTPSETRVAMIEGGILQEIHIEREAKRGIVGNIYKGKVSRVLPGMQAAFVDIGLDKAAFLHASDIVPHTECV
AESEKQQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESEAERERLKKIVSH
YCDEHGGFIIRTAAEGANEKELTQDAAFLKRLWSKVIERRSKYKNRSMLYGELGLAQRILRDFVGTELTKILIDSRQEFE
NLKEFTSEYVPELTAKLELYHGDKPIFDMYDTENEIQRSLERKVELKSGGYLIIDQTEAMTTIDINTGAFVGRRNLEETI
FNTNIEATQAIARQLRLRNLGGIIIIDFIDMASDEHRKRVLTSLETALMKDRVKTNINGFTQLGLVEMTRKRTRESIEHV
LCSTCPTCEGRGSVKTVETVCFEILREITRVNRAYDADKFVVYASPAVADTLQGEESHALAELEVFIGKEVRIQAEPLYN
QEQFDVVMM
>Mature_488_residues
SAELLLNVTPSETRVAMIEGGILQEIHIEREAKRGIVGNIYKGKVSRVLPGMQAAFVDIGLDKAAFLHASDIVPHTECVA
ESEKQQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESEAERERLKKIVSHY
CDEHGGFIIRTAAEGANEKELTQDAAFLKRLWSKVIERRSKYKNRSMLYGELGLAQRILRDFVGTELTKILIDSRQEFEN
LKEFTSEYVPELTAKLELYHGDKPIFDMYDTENEIQRSLERKVELKSGGYLIIDQTEAMTTIDINTGAFVGRRNLEETIF
NTNIEATQAIARQLRLRNLGGIIIIDFIDMASDEHRKRVLTSLETALMKDRVKTNINGFTQLGLVEMTRKRTRESIEHVL
CSTCPTCEGRGSVKTVETVCFEILREITRVNRAYDADKFVVYASPAVADTLQGEESHALAELEVFIGKEVRIQAEPLYNQ
EQFDVVMM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=79.5501022494888, Blast_Score=803, Evalue=0.0,
Organism=Escherichia coli, GI1787325, Length=427, Percent_Identity=36.2997658079625, Blast_Score=276, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55292; Mature: 55161

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAELLLNVTPSETRVAMIEGGILQEIHIEREAKRGIVGNIYKGKVSRVLPGMQAAFVDI
CCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHCC
GLDKAAFLHASDIVPHTECVAESEKQQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIESEAERERLKKIVSHYCDEHGGFIIRTAAEGANEK
EEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
ELTQDAAFLKRLWSKVIERRSKYKNRSMLYGELGLAQRILRDFVGTELTKILIDSRQEFE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
NLKEFTSEYVPELTAKLELYHGDKPIFDMYDTENEIQRSLERKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHEEEEEECCCCCEECCCCHHHHHHHHHHHHEECCCCEEEEECCCCE
TTIDINTGAFVGRRNLEETIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMASDEHRKRV
EEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHCCCHHHHHH
LTSLETALMKDRVKTNINGFTQLGLVEMTRKRTRESIEHVLCSTCPTCEGRGSVKTVETV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CFEILREITRVNRAYDADKFVVYASPAVADTLQGEESHALAELEVFIGKEVRIQAEPLYN
HHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCHHHHHHHHHEECCEEEEEECCCCC
QEQFDVVMM
CCCCCEEEC
>Mature Secondary Structure 
SAELLLNVTPSETRVAMIEGGILQEIHIEREAKRGIVGNIYKGKVSRVLPGMQAAFVDI
CCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHCC
GLDKAAFLHASDIVPHTECVAESEKQQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIESEAERERLKKIVSHYCDEHGGFIIRTAAEGANEK
EEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
ELTQDAAFLKRLWSKVIERRSKYKNRSMLYGELGLAQRILRDFVGTELTKILIDSRQEFE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
NLKEFTSEYVPELTAKLELYHGDKPIFDMYDTENEIQRSLERKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHEEEEEECCCCCEECCCCHHHHHHHHHHHHEECCCCEEEEECCCCE
TTIDINTGAFVGRRNLEETIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMASDEHRKRV
EEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHCCCHHHHHH
LTSLETALMKDRVKTNINGFTQLGLVEMTRKRTRESIEHVLCSTCPTCEGRGSVKTVETV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CFEILREITRVNRAYDADKFVVYASPAVADTLQGEESHALAELEVFIGKEVRIQAEPLYN
HHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCHHHHHHHHHEECCEEEEEECCCCC
QEQFDVVMM
CCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]