Click here to switch to the map view.

The map label for this gene is mshL [H]

Identifier: 227080613

GI number: 227080613

Start: 412434

End: 414113

Strand: Direct

Name: mshL [H]

Synonym: VCM66_0387

Alternate gene names: 227080613

Gene position: 412434-414113 (Clockwise)

Preceding gene: 227080612

Following gene: 227080614

Centisome position: 14.26

GC content: 47.74

Gene sequence:

>1680_bases
ATGCGTAAAATCGTACTCGCTTCAGTGGTGACTTCTTTGGTGGGATGCTCAATGGGACATCGTGATCCTGTTGAAGCTAA
ACAAGCCCTGAACCAAGCTATTAACGAGACGAACAGTCGTCAAATTGACCAATTACCGCCTTCGGTAGAGGCTGATTTGA
TGCCTGATATGGATACTCTTACTGCCAGTGAGCCGAAAACTTTGCAGCGTTTTCGAATCCAAGCTGAGGATGTCGAAGCC
AAGGCCTTTTTTGCCAGTTTAGTGCAGGGAACCGAGTACAGTGCGGCAATCCACCCTGCGGTGACTGGGCGAATTACTCT
CAACTTGACCGACGTTACCCTAGATGAAGCCTTAGGTGTCGTGCGTGATTTGTACGGTTTTGAGGTGGTAAAAGAGGGTA
AAGTGATCCAAGTCTATCCGGCTGGATTGCGTACGGTCACGATTCCGGTTGATTATCTGCAATTTAAACGCACAGGGCGT
TCGTTAACGTCGATTACGACGGGCACCATCACCAATACGGACACCAATAACTCAAATTCAAGTAGTAGCTCCTCGTCCAG
CATCAGTAGTAATAGCTCTTCGGATGGTTCTTCGAGCAATTCTAATTCCAACAGAAGCGATGCTCGTGGCGGAACGGAAA
TTGAAACCACGAACGAGAGTGATTTCTGGCCTTTGTTAGAAAAGGCGGTGGCTCAGTTGCTTGGCGGTAGCGGTGGCCAA
ACGGTCATTGTCAATCCACAGGCGGGAGTATTAACCCTGCGCGCTTATCCCGATGAAATTCGTCAAGTAAACGAGTTTCT
GGGGATCTCGCAACAGCGAATGCATCGACAAGTGATCCTCGAAGCTAAGATTCTTGAAGTGACCCTCAGTGATGGTTACC
AGCAGGGGATTAATTGGAGTAAAGCCTTCTCCTCCAATGGTGCCAATTACAAGATAGGTTCCGGATCCATTACTCAAGAC
AGTAATGGCAATCCTATCACTTCTGTATTACCTGGCTTAGATGCGATAGGTAATTTGTTAGGTGGTCAATCCAATGTGGT
GATCTCCAGTGGCAGCTTTGATGCCGTGATCAGTTTTATGGCGACGCAAGGTGATTTAAATGTTCTGTCTAGCCCGCGAG
TAACTGCGTCCAACAACCAGAAAGCGGTGATCAAAGTCGGGACGGATGAATACTATGTGACCGACTTATCCAGTGTGGTT
GGAACTGGGGATAACGCGCAAGCGTCGCCAGATATTACGCTTACGCCTTTCTTCTCTGGGATTTCATTGGATGTCACGCC
GCAAATTGACGATCAAGGCAACGTATTACTGCATGTGCATCCTGCGGTGATTGAAGTCGAGCAGCAAACCAAGAAAATTT
TATACCGAAGTGAAGAGATTGAGCTGCCATTGGCGAGAAGTTCAATTAGAGAGTCGGATTCGGTTATTCGGGCGAAAGAC
GGCGATGTAGTGGTGATCGGTGGTTTGATGAAGTCAAATACCGTTGACCAAGTGTCAAAAGTGCCATTTTTAGGCGATGT
TCCCGCGTTAGGGCATCTGTTCCGTAACACCACAAAACTGACACAGAAAACAGAACTGGTTATTTTGCTCAAGCCGACGG
TCGTTGGGGTAAATACTTGGCAAAAAGAGCTGGAGCGCTCGCGCAGTTTGCTACAGGAATGGTTCCCGGATAGTCAATAA

Upstream 100 bases:

>100_bases
CAACGATCCATCCCGATCAGGTGAAACTCACCCGGGCAGGTAAAGAGTGGCGATTAGCCCTGTTTACGCTCGATATAAAA
CAATAAGGTAACAGAGTCTT

Downstream 100 bases:

>100_bases
GCGTATGTATTTATCGCACTTTGGCCTTGAACAGTTACCTTTTCATCTGACTCCGGATACCGAATTGTTTCTGGGGCTCG
CACCGCACTTTGAGGCGATT

Product: MSHA biogenesis protein MshL

Products: NA

Alternate protein names: Pectic enzymes secretion protein outD [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MRKIVLASVVTSLVGCSMGHRDPVEAKQALNQAINETNSRQIDQLPPSVEADLMPDMDTLTASEPKTLQRFRIQAEDVEA
KAFFASLVQGTEYSAAIHPAVTGRITLNLTDVTLDEALGVVRDLYGFEVVKEGKVIQVYPAGLRTVTIPVDYLQFKRTGR
SLTSITTGTITNTDTNNSNSSSSSSSSISSNSSSDGSSSNSNSNRSDARGGTEIETTNESDFWPLLEKAVAQLLGGSGGQ
TVIVNPQAGVLTLRAYPDEIRQVNEFLGISQQRMHRQVILEAKILEVTLSDGYQQGINWSKAFSSNGANYKIGSGSITQD
SNGNPITSVLPGLDAIGNLLGGQSNVVISSGSFDAVISFMATQGDLNVLSSPRVTASNNQKAVIKVGTDEYYVTDLSSVV
GTGDNAQASPDITLTPFFSGISLDVTPQIDDQGNVLLHVHPAVIEVEQQTKKILYRSEEIELPLARSSIRESDSVIRAKD
GDVVVIGGLMKSNTVDQVSKVPFLGDVPALGHLFRNTTKLTQKTELVILLKPTVVGVNTWQKELERSRSLLQEWFPDSQ

Sequences:

>Translated_559_residues
MRKIVLASVVTSLVGCSMGHRDPVEAKQALNQAINETNSRQIDQLPPSVEADLMPDMDTLTASEPKTLQRFRIQAEDVEA
KAFFASLVQGTEYSAAIHPAVTGRITLNLTDVTLDEALGVVRDLYGFEVVKEGKVIQVYPAGLRTVTIPVDYLQFKRTGR
SLTSITTGTITNTDTNNSNSSSSSSSSISSNSSSDGSSSNSNSNRSDARGGTEIETTNESDFWPLLEKAVAQLLGGSGGQ
TVIVNPQAGVLTLRAYPDEIRQVNEFLGISQQRMHRQVILEAKILEVTLSDGYQQGINWSKAFSSNGANYKIGSGSITQD
SNGNPITSVLPGLDAIGNLLGGQSNVVISSGSFDAVISFMATQGDLNVLSSPRVTASNNQKAVIKVGTDEYYVTDLSSVV
GTGDNAQASPDITLTPFFSGISLDVTPQIDDQGNVLLHVHPAVIEVEQQTKKILYRSEEIELPLARSSIRESDSVIRAKD
GDVVVIGGLMKSNTVDQVSKVPFLGDVPALGHLFRNTTKLTQKTELVILLKPTVVGVNTWQKELERSRSLLQEWFPDSQ
>Mature_559_residues
MRKIVLASVVTSLVGCSMGHRDPVEAKQALNQAINETNSRQIDQLPPSVEADLMPDMDTLTASEPKTLQRFRIQAEDVEA
KAFFASLVQGTEYSAAIHPAVTGRITLNLTDVTLDEALGVVRDLYGFEVVKEGKVIQVYPAGLRTVTIPVDYLQFKRTGR
SLTSITTGTITNTDTNNSNSSSSSSSSISSNSSSDGSSSNSNSNRSDARGGTEIETTNESDFWPLLEKAVAQLLGGSGGQ
TVIVNPQAGVLTLRAYPDEIRQVNEFLGISQQRMHRQVILEAKILEVTLSDGYQQGINWSKAFSSNGANYKIGSGSITQD
SNGNPITSVLPGLDAIGNLLGGQSNVVISSGSFDAVISFMATQGDLNVLSSPRVTASNNQKAVIKVGTDEYYVTDLSSVV
GTGDNAQASPDITLTPFFSGISLDVTPQIDDQGNVLLHVHPAVIEVEQQTKKILYRSEEIELPLARSSIRESDSVIRAKD
GDVVVIGGLMKSNTVDQVSKVPFLGDVPALGHLFRNTTKLTQKTELVILLKPTVVGVNTWQKELERSRSLLQEWFPDSQ

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=266, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789793, Length=488, Percent_Identity=22.3360655737705, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 60135; Mature: 60135

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIVLASVVTSLVGCSMGHRDPVEAKQALNQAINETNSRQIDQLPPSVEADLMPDMDTL
CCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHC
TASEPKTLQRFRIQAEDVEAKAFFASLVQGTEYSAAIHPAVTGRITLNLTDVTLDEALGV
CCCCCHHHHHHCCCHHCCHHHHHHHHHHCCCCCCEEECCEEEEEEEEEEECCCHHHHHHH
VRDLYGFEVVKEGKVIQVYPAGLRTVTIPVDYLQFKRTGRSLTSITTGTITNTDTNNSNS
HHHHHCCCEECCCCEEEEECCCCEEEEEEHHHHHHHHCCCCEEEEEECEEECCCCCCCCC
SSSSSSSISSNSSSDGSSSNSNSNRSDARGGTEIETTNESDFWPLLEKAVAQLLGGSGGQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCC
TVIVNPQAGVLTLRAYPDEIRQVNEFLGISQQRMHRQVILEAKILEVTLSDGYQQGINWS
EEEECCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHEEEEEEEEECCCHHHCCCHH
KAFSSNGANYKIGSGSITQDSNGNPITSVLPGLDAIGNLLGGQSNVVISSGSFDAVISFM
HHHCCCCCEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHH
ATQGDLNVLSSPRVTASNNQKAVIKVGTDEYYVTDLSSVVGTGDNAQASPDITLTPFFSG
HCCCCCEECCCCCEEECCCCEEEEEECCCCEEEEEHHHHHCCCCCCCCCCCEEEEEECCC
ISLDVTPQIDDQGNVLLHVHPAVIEVEQQTKKILYRSEEIELPLARSSIRESDSVIRAKD
CEEEECCCCCCCCCEEEEECCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEECC
GDVVVIGGLMKSNTVDQVSKVPFLGDVPALGHLFRNTTKLTQKTELVILLKPTVVGVNTW
CCEEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEECCHHH
QKELERSRSLLQEWFPDSQ
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRKIVLASVVTSLVGCSMGHRDPVEAKQALNQAINETNSRQIDQLPPSVEADLMPDMDTL
CCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHC
TASEPKTLQRFRIQAEDVEAKAFFASLVQGTEYSAAIHPAVTGRITLNLTDVTLDEALGV
CCCCCHHHHHHCCCHHCCHHHHHHHHHHCCCCCCEEECCEEEEEEEEEEECCCHHHHHHH
VRDLYGFEVVKEGKVIQVYPAGLRTVTIPVDYLQFKRTGRSLTSITTGTITNTDTNNSNS
HHHHHCCCEECCCCEEEEECCCCEEEEEEHHHHHHHHCCCCEEEEEECEEECCCCCCCCC
SSSSSSSISSNSSSDGSSSNSNSNRSDARGGTEIETTNESDFWPLLEKAVAQLLGGSGGQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCC
TVIVNPQAGVLTLRAYPDEIRQVNEFLGISQQRMHRQVILEAKILEVTLSDGYQQGINWS
EEEECCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHEEEEEEEEECCCHHHCCCHH
KAFSSNGANYKIGSGSITQDSNGNPITSVLPGLDAIGNLLGGQSNVVISSGSFDAVISFM
HHHCCCCCEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHH
ATQGDLNVLSSPRVTASNNQKAVIKVGTDEYYVTDLSSVVGTGDNAQASPDITLTPFFSG
HCCCCCEECCCCCEEECCCCEEEEEECCCCEEEEEHHHHHCCCCCCCCCCCEEEEEECCC
ISLDVTPQIDDQGNVLLHVHPAVIEVEQQTKKILYRSEEIELPLARSSIRESDSVIRAKD
CEEEECCCCCCCCCEEEEECCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEECC
GDVVVIGGLMKSNTVDQVSKVPFLGDVPALGHLFRNTTKLTQKTELVILLKPTVVGVNTW
CCEEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEECCHHH
QKELERSRSLLQEWFPDSQ
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]