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The map label for this gene is hcaT [H]

Identifier: 227080516

GI number: 227080516

Start: 294720

End: 295910

Strand: Direct

Name: hcaT [H]

Synonym: VCM66_0287

Alternate gene names: 227080516

Gene position: 294720-295910 (Clockwise)

Preceding gene: 227080502

Following gene: 227080517

Centisome position: 10.19

GC content: 53.48

Gene sequence:

>1191_bases
ATGTTTACCCCCACTCCCTATGGTTGGATTTCGCAATATTTCATCGGTTTTTTCTTTGCTTACGGCGTGTATTTGCCATT
TTGGTCACTCTGGTTTGAAGACCAAGGCGTTTCAGCGACCGACATAGGTGTCTTGGTTGGGATAGGCTTTGCGACGCGCT
GTGTAGCGAATTTGGTGCTGACGCCGCGTTTACACCGTGTTGAGCAACTGATGCCCGCGCTGCGTTGGCTCAGTTTCGCG
GCAATGCTGTTTGTCGGTTTTCACTTTTTTACGGGCGGCAGTTTTTGGCTGATGGCGCTGGCCACTGTGCTGTTTAACTT
ATGCTGTGGCCCTGTGGTGCCGATTTCTGATGCCATGGCCAACTACTACGCCAAGCTGAAAATGCTCGACTATGGCCGTA
CTCGTTTGTGGGGGTCGATCGCGTTTATTGCCGGATCGACTGTGGTCGGATTCTTGGTCGCCAAGTGGGGCAGCTTGATG
ATTTTGTACACGGCGCTGGTGGGGGTCGCCGTCTCTTGGCTGTTGAGTTTGCGCCAAATTACACCGATGCCAGTCACTGA
GCACGAGGCTCATGCGGTGCGCCCGAAGCTGAAGGAGCTTCTTACCGAGTGGCCTGTGGTGAAATTTCTGCTGCTGGTGG
CCTTAATCCAAGGCAGTCATGCGGCGTATTACAGTTTCGGCTCGATTTATTGGAAACAAGCGGGTCACTCAGAAGATATC
ATCGGTTACTTATGGAGCTTAGGTGTGGTCGCGGAAGTCTTGGTGTTTGCTTTCAGTAAGCGCTGGTTCGCGGGCTGGAG
TTTGCGCACACTATTCTTCGTAGCGTCGTTGGGGGTTATTGCACGTTGGGGTTTAACCGCCTCCACGACCACCATTCTGG
CCTTAGTGGCGATTCAACTGCTGCATGGGGTCACGTTTGCTATCGCGCACATCGCCGCGATTCAGTACATCCAGCATGCG
CCGCAAAATAAAATGGTGGCACTGCAAGCTCTGTATAACGCGATTCCACTCGGCGCGGTGATTGCGGCGGTGACCACTCT
CAGTGGTTGGGGCTATGAGCATTGGGGCGCAGCCGTATTCTGGGCGATGGCCGCAATGGGCGGATTGGCATTATTGATCC
GCGTTGATACTCCGCCTTCAACGGTACAAGATGGCAATGCACTGAAAGCGGAGCCTGAAGCACAGAATTAA

Upstream 100 bases:

>100_bases
AATCGGGAAACGATGAGTTGCGTAATCTTCCCTTTTTTAGACGCGAATCCACATGCATAATGTGCGCGCTTTATACCTCC
CTTTTTGATTAGGAACGATC

Downstream 100 bases:

>100_bases
GTCGTGCTGATTGAGTTCAAAAAACAACCTCGTTTTAATAGAAGCCTCTCAATCGAGAGGCTTTTTTCATTTCGCAACAG
CAAGGATGTCTGATGCAAGG

Product: putative 3-phenylpropionic acid transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MFTPTPYGWISQYFIGFFFAYGVYLPFWSLWFEDQGVSATDIGVLVGIGFATRCVANLVLTPRLHRVEQLMPALRWLSFA
AMLFVGFHFFTGGSFWLMALATVLFNLCCGPVVPISDAMANYYAKLKMLDYGRTRLWGSIAFIAGSTVVGFLVAKWGSLM
ILYTALVGVAVSWLLSLRQITPMPVTEHEAHAVRPKLKELLTEWPVVKFLLLVALIQGSHAAYYSFGSIYWKQAGHSEDI
IGYLWSLGVVAEVLVFAFSKRWFAGWSLRTLFFVASLGVIARWGLTASTTTILALVAIQLLHGVTFAIAHIAAIQYIQHA
PQNKMVALQALYNAIPLGAVIAAVTTLSGWGYEHWGAAVFWAMAAMGGLALLIRVDTPPSTVQDGNALKAEPEAQN

Sequences:

>Translated_396_residues
MFTPTPYGWISQYFIGFFFAYGVYLPFWSLWFEDQGVSATDIGVLVGIGFATRCVANLVLTPRLHRVEQLMPALRWLSFA
AMLFVGFHFFTGGSFWLMALATVLFNLCCGPVVPISDAMANYYAKLKMLDYGRTRLWGSIAFIAGSTVVGFLVAKWGSLM
ILYTALVGVAVSWLLSLRQITPMPVTEHEAHAVRPKLKELLTEWPVVKFLLLVALIQGSHAAYYSFGSIYWKQAGHSEDI
IGYLWSLGVVAEVLVFAFSKRWFAGWSLRTLFFVASLGVIARWGLTASTTTILALVAIQLLHGVTFAIAHIAAIQYIQHA
PQNKMVALQALYNAIPLGAVIAAVTTLSGWGYEHWGAAVFWAMAAMGGLALLIRVDTPPSTVQDGNALKAEPEAQN
>Mature_396_residues
MFTPTPYGWISQYFIGFFFAYGVYLPFWSLWFEDQGVSATDIGVLVGIGFATRCVANLVLTPRLHRVEQLMPALRWLSFA
AMLFVGFHFFTGGSFWLMALATVLFNLCCGPVVPISDAMANYYAKLKMLDYGRTRLWGSIAFIAGSTVVGFLVAKWGSLM
ILYTALVGVAVSWLLSLRQITPMPVTEHEAHAVRPKLKELLTEWPVVKFLLLVALIQGSHAAYYSFGSIYWKQAGHSEDI
IGYLWSLGVVAEVLVFAFSKRWFAGWSLRTLFFVASLGVIARWGLTASTTTILALVAIQLLHGVTFAIAHIAAIQYIQHA
PQNKMVALQALYNAIPLGAVIAAVTTLSGWGYEHWGAAVFWAMAAMGGLALLIRVDTPPSTVQDGNALKAEPEAQN

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=377, Percent_Identity=38.7267904509284, Blast_Score=245, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 43561; Mature: 43561

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTPTPYGWISQYFIGFFFAYGVYLPFWSLWFEDQGVSATDIGVLVGIGFATRCVANLVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TPRLHRVEQLMPALRWLSFAAMLFVGFHFFTGGSFWLMALATVLFNLCCGPVVPISDAMA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
NYYAKLKMLDYGRTRLWGSIAFIAGSTVVGFLVAKWGSLMILYTALVGVAVSWLLSLRQI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPMPVTEHEAHAVRPKLKELLTEWPVVKFLLLVALIQGSHAAYYSFGSIYWKQAGHSEDI
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
IGYLWSLGVVAEVLVFAFSKRWFAGWSLRTLFFVASLGVIARWGLTASTTTILALVAIQL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LHGVTFAIAHIAAIQYIQHAPQNKMVALQALYNAIPLGAVIAAVTTLSGWGYEHWGAAVF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
WAMAAMGGLALLIRVDTPPSTVQDGNALKAEPEAQN
HHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]