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The map label for this gene is edd [H]

Identifier: 227080502

GI number: 227080502

Start: 281547

End: 283403

Strand: Direct

Name: edd [H]

Synonym: VCM66_0273

Alternate gene names: 227080502

Gene position: 281547-283403 (Clockwise)

Preceding gene: 227080501

Following gene: 227080516

Centisome position: 9.73

GC content: 54.5

Gene sequence:

>1857_bases
TTGTTCAGTGCAGGAAGTGGTGAATTGTGCGATTCAAGCACTGCAAGAGCAGGAGGGTCTATGATTCATCCTGTTTTAGC
CAAAGTTACTGAAAATCTCATCGAGCGTAGCCGTGAAGCACGTGCGGCGTTTATTGCACGCAGTCAAGCACAAGAAAAAG
CCGGTAAAGGTCGCACCTCACTCTCTTGCGGTAATTTAGCCCACGCAGTGGCGGCTTCTTGCAGCACAGAGAAAAAAGCG
ATCCTCGATTTTACGCGCAGTAATGTGGCGATTGTAACTTCCTATAACGATATGCTCAGCGCGCATCAGCCTTATCAGCA
CTATCCAGACCGCATCAAGATGGTGTTAGCCGAGTACGGCCATACCGCGCAGGTCGCCGGTGGCGTACCCGCCATGTGCG
ATGGCGTGACCCAAGGTCAGCCGGGGATGGATATGTCGCTGTTTTCCCGTGATTTGATTGCGCAGGCGACCGCACTCTCC
CTCAGTCACAATTTGTTTGATGCCACTTTACTGCTCGGTATTTGTGACAAAATCGCGCCGGGGCAATTGATGGGCGCGCT
TTCTTACGCCCATCTGCCGACCGCGTTTATGCCTGCGGGTTTAATGGCAACCGGCATCAGTAACGAAGAGAAAGTGGATA
TTCGCCAAAAGTATGCGGCGGGTGAAGTGGGGAAAGAGGCGCTACTGAATATGGAGTGTCAGGCTTACCACGCGCCGGGT
ACGTGTACTTTCTACGGCACTGCCAACACCAACCAATTGGTGTTTGAAGCCATGGGGCTGATGTTGCCGGGCTCGGCGTT
CGTGCATCCTCATTCGGCGCTGCGCCAAGCTCTCAACGATGACGCGACGGTAAAAATTGCCAGCATGACCCAAGGCTCAG
CGCAGTATCGTCCGCTCTATCAAGTGGTCACCGAGCAAAGCCTACTCAATGGGATTGTTGCTCTGCTGGCTTCTGGTGGC
AGTACGAACCACACCATTCATATGTTGGCGGTGGCACGTGCCGCGGGGATTTTGCTGACCTGGCAGGACATCAGTGATCT
GTCGGATGTGGTTCCGCTGCTGGCGAAGGTTTACCCGAATGGCCCCGCGGATATGAATGCCTTCCAACAAGCGGGTGGTG
TACCCGCACTGATGAAGCGTTTGCATGAAGCCAAGTTGCTGCACTCGGATGTCACACCGGTATTTGGTGAGTTTAGTGAT
CAACTGACGCTGCCTGAGTTGGTTGCGGGGGAGCTGCGTTGGATGGTGTGCGAAGGCACGCGTGATGCGCAGGTGATCGC
GGCGAGTGGCGAGTGCTTCCAAACCACTGGCGGCACTAAAATGCTGAACGGGAACTTAGGCCGCGCAGTGATCAAAGTCT
CTGCGGTGAAAGAAGAACAACGCATCATTGAAGCGCCCGCGGTGGTATTTCAGTGTCAGCATGAAGTGGAAGCGGCGTAC
CAGCGTGGCGAACTCAATAAAGACTGCATCGTGGTGGTGACCCACAATGGCCCCGCGGCGAATGGCATGCCTGAATTGCA
CAAATTGATGCCGATTTTGGGCAACGTGCAAAAAGCCGGATTCAAAGTCGCGCTGGTGACCGATGGCCGTTTGTCGGGCG
CGTCAGGTAAAATTCCATCGGCGATTCACGTGTCACCAGAAGCGGTACGCGGCGGTGCGATTGGCTTAGTGCGTGATGGG
GATTTGCTGCGCTTGGATTGCACAACAGGCACGCTTGAGAACTTAACCGACATGAGCCATCGCCAAGCTCTCGCTTTAGA
TACCGAGCGCGATCAGCAAATGTGGGGACGCGAATTGTTTAAAGTGATGCGCCAAGCGGTCTCTTCCGCCGAACAAGGGG
CGAGCTTTATTGTTTAA

Upstream 100 bases:

>100_bases
CTCGCCAAGGCCATTTCATGAAAGAGAACATGGTCAACAGTCAGTTTGAAACCTTAGAGCGCCCTGATGGTGAGCCGCAA
ACCCTGATCATCCCTATTGA

Downstream 100 bases:

>100_bases
CCTTGCTGGATGTACAACATAGAAAAGGCGGTGTATACCGCCTTTTTCATCTTTATCATTTGTGGAGTGCTGGTGTTACA
CACTTTCACCCGCAGTAAAA

Product: phosphogluconate dehydratase

Products: NA

Alternate protein names: 6-phosphogluconate dehydratase [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MFSAGSGELCDSSTARAGGSMIHPVLAKVTENLIERSREARAAFIARSQAQEKAGKGRTSLSCGNLAHAVAASCSTEKKA
ILDFTRSNVAIVTSYNDMLSAHQPYQHYPDRIKMVLAEYGHTAQVAGGVPAMCDGVTQGQPGMDMSLFSRDLIAQATALS
LSHNLFDATLLLGICDKIAPGQLMGALSYAHLPTAFMPAGLMATGISNEEKVDIRQKYAAGEVGKEALLNMECQAYHAPG
TCTFYGTANTNQLVFEAMGLMLPGSAFVHPHSALRQALNDDATVKIASMTQGSAQYRPLYQVVTEQSLLNGIVALLASGG
STNHTIHMLAVARAAGILLTWQDISDLSDVVPLLAKVYPNGPADMNAFQQAGGVPALMKRLHEAKLLHSDVTPVFGEFSD
QLTLPELVAGELRWMVCEGTRDAQVIAASGECFQTTGGTKMLNGNLGRAVIKVSAVKEEQRIIEAPAVVFQCQHEVEAAY
QRGELNKDCIVVVTHNGPAANGMPELHKLMPILGNVQKAGFKVALVTDGRLSGASGKIPSAIHVSPEAVRGGAIGLVRDG
DLLRLDCTTGTLENLTDMSHRQALALDTERDQQMWGRELFKVMRQAVSSAEQGASFIV

Sequences:

>Translated_618_residues
MFSAGSGELCDSSTARAGGSMIHPVLAKVTENLIERSREARAAFIARSQAQEKAGKGRTSLSCGNLAHAVAASCSTEKKA
ILDFTRSNVAIVTSYNDMLSAHQPYQHYPDRIKMVLAEYGHTAQVAGGVPAMCDGVTQGQPGMDMSLFSRDLIAQATALS
LSHNLFDATLLLGICDKIAPGQLMGALSYAHLPTAFMPAGLMATGISNEEKVDIRQKYAAGEVGKEALLNMECQAYHAPG
TCTFYGTANTNQLVFEAMGLMLPGSAFVHPHSALRQALNDDATVKIASMTQGSAQYRPLYQVVTEQSLLNGIVALLASGG
STNHTIHMLAVARAAGILLTWQDISDLSDVVPLLAKVYPNGPADMNAFQQAGGVPALMKRLHEAKLLHSDVTPVFGEFSD
QLTLPELVAGELRWMVCEGTRDAQVIAASGECFQTTGGTKMLNGNLGRAVIKVSAVKEEQRIIEAPAVVFQCQHEVEAAY
QRGELNKDCIVVVTHNGPAANGMPELHKLMPILGNVQKAGFKVALVTDGRLSGASGKIPSAIHVSPEAVRGGAIGLVRDG
DLLRLDCTTGTLENLTDMSHRQALALDTERDQQMWGRELFKVMRQAVSSAEQGASFIV
>Mature_618_residues
MFSAGSGELCDSSTARAGGSMIHPVLAKVTENLIERSREARAAFIARSQAQEKAGKGRTSLSCGNLAHAVAASCSTEKKA
ILDFTRSNVAIVTSYNDMLSAHQPYQHYPDRIKMVLAEYGHTAQVAGGVPAMCDGVTQGQPGMDMSLFSRDLIAQATALS
LSHNLFDATLLLGICDKIAPGQLMGALSYAHLPTAFMPAGLMATGISNEEKVDIRQKYAAGEVGKEALLNMECQAYHAPG
TCTFYGTANTNQLVFEAMGLMLPGSAFVHPHSALRQALNDDATVKIASMTQGSAQYRPLYQVVTEQSLLNGIVALLASGG
STNHTIHMLAVARAAGILLTWQDISDLSDVVPLLAKVYPNGPADMNAFQQAGGVPALMKRLHEAKLLHSDVTPVFGEFSD
QLTLPELVAGELRWMVCEGTRDAQVIAASGECFQTTGGTKMLNGNLGRAVIKVSAVKEEQRIIEAPAVVFQCQHEVEAAY
QRGELNKDCIVVVTHNGPAANGMPELHKLMPILGNVQKAGFKVALVTDGRLSGASGKIPSAIHVSPEAVRGGAIGLVRDG
DLLRLDCTTGTLENLTDMSHRQALALDTERDQQMWGRELFKVMRQAVSSAEQGASFIV

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI1788157, Length=601, Percent_Identity=55.9068219633943, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI48994964, Length=537, Percent_Identity=29.9813780260708, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI2367371, Length=531, Percent_Identity=29.7551789077213, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1786464, Length=473, Percent_Identity=31.0782241014799, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322476, Length=474, Percent_Identity=29.3248945147679, Blast_Score=161, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004786
- InterPro:   IPR015928
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.12 [H]

Molecular weight: Translated: 65783; Mature: 65783

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSAGSGELCDSSTARAGGSMIHPVLAKVTENLIERSREARAAFIARSQAQEKAGKGRTS
CCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LSCGNLAHAVAASCSTEKKAILDFTRSNVAIVTSYNDMLSAHQPYQHYPDRIKMVLAEYG
CCCCHHHHHHHHHCCCCHHHHHEECCCCEEEEEECHHHHHCCCCHHHCHHHHHHHHHHCC
HTAQVAGGVPAMCDGVTQGQPGMDMSLFSRDLIAQATALSLSHNLFDATLLLGICDKIAP
CCEECCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GQLMGALSYAHLPTAFMPAGLMATGISNEEKVDIRQKYAAGEVGKEALLNMECQAYHAPG
HHHHHHHHHHHCCHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
TCTFYGTANTNQLVFEAMGLMLPGSAFVHPHSALRQALNDDATVKIASMTQGSAQYRPLY
EEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
QVVTEQSLLNGIVALLASGGSTNHTIHMLAVARAAGILLTWQDISDLSDVVPLLAKVYPN
HHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCC
GPADMNAFQQAGGVPALMKRLHEAKLLHSDVTPVFGEFSDQLTLPELVAGELRWMVCEGT
CCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHCCEEEEEECCC
RDAQVIAASGECFQTTGGTKMLNGNLGRAVIKVSAVKEEQRIIEAPAVVFQCQHEVEAAY
CCCEEEEECCCEEECCCCCEEECCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHH
QRGELNKDCIVVVTHNGPAANGMPELHKLMPILGNVQKAGFKVALVTDGRLSGASGKIPS
HCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHCCEEEEEEECCCCCCCCCCCCC
AIHVSPEAVRGGAIGLVRDGDLLRLDCTTGTLENLTDMSHRQALALDTERDQQMWGRELF
EEECCHHHHCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHEEEECCHHHHHHHHHHHH
KVMRQAVSSAEQGASFIV
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFSAGSGELCDSSTARAGGSMIHPVLAKVTENLIERSREARAAFIARSQAQEKAGKGRTS
CCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LSCGNLAHAVAASCSTEKKAILDFTRSNVAIVTSYNDMLSAHQPYQHYPDRIKMVLAEYG
CCCCHHHHHHHHHCCCCHHHHHEECCCCEEEEEECHHHHHCCCCHHHCHHHHHHHHHHCC
HTAQVAGGVPAMCDGVTQGQPGMDMSLFSRDLIAQATALSLSHNLFDATLLLGICDKIAP
CCEECCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GQLMGALSYAHLPTAFMPAGLMATGISNEEKVDIRQKYAAGEVGKEALLNMECQAYHAPG
HHHHHHHHHHHCCHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
TCTFYGTANTNQLVFEAMGLMLPGSAFVHPHSALRQALNDDATVKIASMTQGSAQYRPLY
EEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
QVVTEQSLLNGIVALLASGGSTNHTIHMLAVARAAGILLTWQDISDLSDVVPLLAKVYPN
HHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCC
GPADMNAFQQAGGVPALMKRLHEAKLLHSDVTPVFGEFSDQLTLPELVAGELRWMVCEGT
CCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHCCEEEEEECCC
RDAQVIAASGECFQTTGGTKMLNGNLGRAVIKVSAVKEEQRIIEAPAVVFQCQHEVEAAY
CCCEEEEECCCEEECCCCCEEECCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHH
QRGELNKDCIVVVTHNGPAANGMPELHKLMPILGNVQKAGFKVALVTDGRLSGASGKIPS
HCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHCCEEEEEEECCCCCCCCCCCCC
AIHVSPEAVRGGAIGLVRDGDLLRLDCTTGTLENLTDMSHRQALALDTERDQQMWGRELF
EEECCHHHHCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHEEEECCHHHHHHHHHHHH
KVMRQAVSSAEQGASFIV
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]