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The map label for this gene is proA [H]

Identifier: 227080391

GI number: 227080391

Start: 147249

End: 148856

Strand: Reverse

Name: proA [H]

Synonym: VCM66_0156

Alternate gene names: 227080391

Gene position: 148856-147249 (Counterclockwise)

Preceding gene: 227080393

Following gene: 227080389

Centisome position: 5.15

GC content: 46.64

Gene sequence:

>1608_bases
ATGTTTAAAAAGTTTTTAAGCTTATGCATTGTTTCGACGTTTTCCGTCGCAGCAACCTCAGCACTTGCCCAACCCAATCA
GCTTGTTGGCAAATCATCTCCTCAACAATTAGCACCATTGATGAAAGCCGCTTCAGGGAAAGGCATTAAAAATCAATACA
TTGTTGTACTCAAGCAACCAACGACAATCATGAGTAATGATTTGCAAGCTTTCCAACAATTTACTCAACGATCTGTCAAT
GCATTAGCCAATAAGCATGCACTCGAAATCAAAAATGTTTTTGATAGTGCGTTAAGCGGTTTTTCTGCAGAGCTAACGGC
TGAGCAACTACAAGCTCTTCGTGCCGATCCTAACGTCGACTATATCGAGCAAAACCAAATTATTACGGTTAATCCGATCA
TCTCTGCATCAGCAAATGCGGCTCAAGATAACGTAACCTGGGGAATCGACCGCATCGATCAGCGCGATCTACCGCTCAAT
CGTAGTTATAACTACAACTATGATGGCAGTGGCGTTACCGCTTATGTGATCGATACCGGTATTGCTTTCAACCATCCTGA
ATTTGGTGGACGAGCAAAATCCGGCTATGACTTTATCGATAACGATAATGATGCCAGTGACTGCCAAGGCCACGGTACAC
ATGTGGCAGGCACTATCGGGGGCGCTCAATACGGTGTAGCTAAAAACGTTAACTTAGTTGGTGTGCGAGTGCTGGGATGT
GATGGTAGTGGTTCAACTGAAGCGATCGCCCGTGGTATTGACTGGGTGGCTCAAAACGCGTCCGGCCCTTCTGTTGCCAA
TTTGAGTTTAGGGGGAGGGATATCTCAGGCGATGGATCAAGCAGTGGCTCGACTCGTCCAAAGAGGAGTCACGGCGGTCA
TTGCTGCTGGTAACGATAATAAAGATGCTTGCCAAGTATCCCCTGCACGTGAACCAAGTGGTATCACTGTAGGTTCAACG
ACCAATAATGATGGTCGCTCTAACTTCTCGAACTGGGGTAATTGTGTACAGATCTTCGCACCAGGATCTGACGTCACTTC
GGCGTCGCATAAAGGTGGCACGACGACTATGAGTGGTACGTCTATGGCCTCACCCCATGTAGCTGGTGTCGCAGCCTTGT
ACTTACAAGAGAATAAGAACCTCTCTCCGAATCAAATCAAAACGCTGCTTAGCGACCGATCAACCAAAGGCAAAGTCAGC
GACACTCAAGGAACACCAAATAAGCTGCTGTATAGTTTGACCGATAACAATACCACTCCGAATCCTGAGCCAAATCCGCA
ACCAGAGCCACAACCGCAGCCTGACAGCCAATTGACTAATGGTAAAGTGGTTACAGGCATCAGTGGCAAGCAAGGTGAAT
TGAAAAAATTCTATATTGATGTGCCTGCAGGTCGTCGCTTGAGTATCGAGACCAACGGAGGTACGGGTAATCTTGATCTC
TATGTTCGTCTAGGTATTGAGCCAGAACCGTTTGCTTGGGATTGCGCATCTTATCGCAACGGCAATAATGAAGTTTGTAC
CTTCCCGAATACCCGAGAAGGTCGCCACTTCATTACACTCTATGGCACCACTGAGTTTAACAATGTCAGTTTGGTGGCTC
GCTACTAA

Upstream 100 bases:

>100_bases
CTTCTTTTCATCCAAGGATGAGATCGTTATTGGTGTGCTCAACATGATTGTTGTGAGCCACGACCCAACCATTTTTATTC
ACTTTTTAAGGAAACTTATC

Downstream 100 bases:

>100_bases
CATGACGCATTAAACAATAAAAGCCAGTCAGATGTTCTGACTGGCTTTTGTGATTAAAGACAGTGTCTAAGTTTAAAACT
GATAACCAATATTAAGATAA

Product: alkaline serine protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MFKKFLSLCIVSTFSVAATSALAQPNQLVGKSSPQQLAPLMKAASGKGIKNQYIVVLKQPTTIMSNDLQAFQQFTQRSVN
ALANKHALEIKNVFDSALSGFSAELTAEQLQALRADPNVDYIEQNQIITVNPIISASANAAQDNVTWGIDRIDQRDLPLN
RSYNYNYDGSGVTAYVIDTGIAFNHPEFGGRAKSGYDFIDNDNDASDCQGHGTHVAGTIGGAQYGVAKNVNLVGVRVLGC
DGSGSTEAIARGIDWVAQNASGPSVANLSLGGGISQAMDQAVARLVQRGVTAVIAAGNDNKDACQVSPAREPSGITVGST
TNNDGRSNFSNWGNCVQIFAPGSDVTSASHKGGTTTMSGTSMASPHVAGVAALYLQENKNLSPNQIKTLLSDRSTKGKVS
DTQGTPNKLLYSLTDNNTTPNPEPNPQPEPQPQPDSQLTNGKVVTGISGKQGELKKFYIDVPAGRRLSIETNGGTGNLDL
YVRLGIEPEPFAWDCASYRNGNNEVCTFPNTREGRHFITLYGTTEFNNVSLVARY

Sequences:

>Translated_535_residues
MFKKFLSLCIVSTFSVAATSALAQPNQLVGKSSPQQLAPLMKAASGKGIKNQYIVVLKQPTTIMSNDLQAFQQFTQRSVN
ALANKHALEIKNVFDSALSGFSAELTAEQLQALRADPNVDYIEQNQIITVNPIISASANAAQDNVTWGIDRIDQRDLPLN
RSYNYNYDGSGVTAYVIDTGIAFNHPEFGGRAKSGYDFIDNDNDASDCQGHGTHVAGTIGGAQYGVAKNVNLVGVRVLGC
DGSGSTEAIARGIDWVAQNASGPSVANLSLGGGISQAMDQAVARLVQRGVTAVIAAGNDNKDACQVSPAREPSGITVGST
TNNDGRSNFSNWGNCVQIFAPGSDVTSASHKGGTTTMSGTSMASPHVAGVAALYLQENKNLSPNQIKTLLSDRSTKGKVS
DTQGTPNKLLYSLTDNNTTPNPEPNPQPEPQPQPDSQLTNGKVVTGISGKQGELKKFYIDVPAGRRLSIETNGGTGNLDL
YVRLGIEPEPFAWDCASYRNGNNEVCTFPNTREGRHFITLYGTTEFNNVSLVARY
>Mature_535_residues
MFKKFLSLCIVSTFSVAATSALAQPNQLVGKSSPQQLAPLMKAASGKGIKNQYIVVLKQPTTIMSNDLQAFQQFTQRSVN
ALANKHALEIKNVFDSALSGFSAELTAEQLQALRADPNVDYIEQNQIITVNPIISASANAAQDNVTWGIDRIDQRDLPLN
RSYNYNYDGSGVTAYVIDTGIAFNHPEFGGRAKSGYDFIDNDNDASDCQGHGTHVAGTIGGAQYGVAKNVNLVGVRVLGC
DGSGSTEAIARGIDWVAQNASGPSVANLSLGGGISQAMDQAVARLVQRGVTAVIAAGNDNKDACQVSPAREPSGITVGST
TNNDGRSNFSNWGNCVQIFAPGSDVTSASHKGGTTTMSGTSMASPHVAGVAALYLQENKNLSPNQIKTLLSDRSTKGKVS
DTQGTPNKLLYSLTDNNTTPNPEPNPQPEPQPQPDSQLTNGKVVTGISGKQGELKKFYIDVPAGRRLSIETNGGTGNLDL
YVRLGIEPEPFAWDCASYRNGNNEVCTFPNTREGRHFITLYGTTEFNNVSLVARY

Specific function: Unknown

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI31317307, Length=370, Percent_Identity=32.4324324324324, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4505579, Length=356, Percent_Identity=24.438202247191, Blast_Score=67, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320775, Length=430, Percent_Identity=37.906976744186, Blast_Score=241, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6324576, Length=405, Percent_Identity=35.8024691358025, Blast_Score=227, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6319893, Length=277, Percent_Identity=40.7942238267148, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI45550681, Length=250, Percent_Identity=26.4, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007280
- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8; PF04151 PPC [H]

EC number: NA

Molecular weight: Translated: 56862; Mature: 56862

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER ; PS00879 ODR_DC_2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKFLSLCIVSTFSVAATSALAQPNQLVGKSSPQQLAPLMKAASGKGIKNQYIVVLKQP
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
TTIMSNDLQAFQQFTQRSVNALANKHALEIKNVFDSALSGFSAELTAEQLQALRADPNVD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
YIEQNQIITVNPIISASANAAQDNVTWGIDRIDQRDLPLNRSYNYNYDGSGVTAYVIDTG
EECCCCEEEECCCEECCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
IAFNHPEFGGRAKSGYDFIDNDNDASDCQGHGTHVAGTIGGAQYGVAKNVNLVGVRVLGC
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEEC
DGSGSTEAIARGIDWVAQNASGPSVANLSLGGGISQAMDQAVARLVQRGVTAVIAAGNDN
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCC
KDACQVSPAREPSGITVGSTTNNDGRSNFSNWGNCVQIFAPGSDVTSASHKGGTTTMSGT
CCCEEECCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCC
SMASPHVAGVAALYLQENKNLSPNQIKTLLSDRSTKGKVSDTQGTPNKLLYSLTDNNTTP
CCCCCCHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCC
NPEPNPQPEPQPQPDSQLTNGKVVTGISGKQGELKKFYIDVPAGRRLSIETNGGTGNLDL
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEECCCCCCEEE
YVRLGIEPEPFAWDCASYRNGNNEVCTFPNTREGRHFITLYGTTEFNNVSLVARY
EEEECCCCCCCEECCHHHCCCCCCEEECCCCCCCCEEEEEEECCCCCCEEEEEEC
>Mature Secondary Structure
MFKKFLSLCIVSTFSVAATSALAQPNQLVGKSSPQQLAPLMKAASGKGIKNQYIVVLKQP
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
TTIMSNDLQAFQQFTQRSVNALANKHALEIKNVFDSALSGFSAELTAEQLQALRADPNVD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
YIEQNQIITVNPIISASANAAQDNVTWGIDRIDQRDLPLNRSYNYNYDGSGVTAYVIDTG
EECCCCEEEECCCEECCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
IAFNHPEFGGRAKSGYDFIDNDNDASDCQGHGTHVAGTIGGAQYGVAKNVNLVGVRVLGC
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEEC
DGSGSTEAIARGIDWVAQNASGPSVANLSLGGGISQAMDQAVARLVQRGVTAVIAAGNDN
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCC
KDACQVSPAREPSGITVGSTTNNDGRSNFSNWGNCVQIFAPGSDVTSASHKGGTTTMSGT
CCCEEECCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCC
SMASPHVAGVAALYLQENKNLSPNQIKTLLSDRSTKGKVSDTQGTPNKLLYSLTDNNTTP
CCCCCCHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCC
NPEPNPQPEPQPQPDSQLTNGKVVTGISGKQGELKKFYIDVPAGRRLSIETNGGTGNLDL
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEECCCCCCEEE
YVRLGIEPEPFAWDCASYRNGNNEVCTFPNTREGRHFITLYGTTEFNNVSLVARY
EEEECCCCCCCEECCHHHCCCCCCEEECCCCCCCCEEEEEEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2546861 [H]