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The map label for this gene is trmA

Identifier: 227080389

GI number: 227080389

Start: 143834

End: 144943

Strand: Reverse

Name: trmA

Synonym: VCM66_0154

Alternate gene names: 227080389

Gene position: 144943-143834 (Counterclockwise)

Preceding gene: 227080391

Following gene: 227080386

Centisome position: 5.01

GC content: 47.84

Gene sequence:

>1110_bases
ATGGCGACTCTTGATGTAAACCCTGAACTCTACCAAGCTCAATTGGCAGACAAAATCGCCCGTTTAAAAGCGATGTTTGT
TGATTACTCCATGCCTGAGCTGGAAGTGTTTGAGTCGCCAGTGGCGAATTATCGTATGCGTGCTGAATTTCGGATTTGGC
ATGAAGGCGATGACATGTACTACATCATGTTTAATCAAGAGACGCGCGAAAAGTACCGCGTGGATCAATTTCCAGCGGCG
AGCCGCCTGATTAACGATTTGATGCCTCTGCTGATGGATGCGATGAAAGGCAGCCCTATTCTGCGCCATAAACTGTTTCA
GGTTGATTTCCTCTCAACCCTCAGTGGCGAGATTTTGGTTTCACTGCTGTACCATCGACAGCTCAGCGAAGAATGGATCA
CTGCGGCGCAAGCACTCAAACAGCGCCTAAACGATGAAGGGTTCAACCTGAACTTAATCGGCCGTGCTCGCAAAATGAAA
GTAGTGCTGGATCGTGATTACGTGGTGGAGAATTTACAGGTCAATGGCCAGCCTTATGTATATAAGCAAGTCGAAAACAG
TTTTACCCAACCTAATGCGAAGGTCGCCGAAAAAATGTTGGAGTGGGCGGTCGATTGTACTCAAGAGAGCAAGGGGGATT
TGCTTGAACTGTATTGCGGTAACGGCAACTTCTCACTCGCGTTAGCGCAAAACTTTGAGCGTGTACTGGCAACCGAATTA
GCCAAACCTTCTGTAGAAGCCGCGCAATTTAATATTGCCGCGAACCAGATCGGCAACGTGCAGATCATTCGCATGTCAGC
AGAAGAGTTTACTCAGGCGATGGAAGGTAAACGCGAATTCAATCGTTTGAAGGATGCGGGAGTCGATCTGCAAAGCTACC
GTTGCAACACTATTTTTGTCGACCCACCACGCTCAGGCATGGATATCGACACTTGTAAAATGGTGCAAGGTTATGAGCGC
ATTCTGTACATCTCTTGTAACCCAGAAACGCTGCAAGAGAACTTACAAGTCTTGGGGGAAACCCATCAAGTGGTGCGCTT
TGCTCTCTTCGACCAATTTCCTTACACCCACCACATGGAAGCGGGCGTGATGTTAGAGCGCAAAAAGTAA

Upstream 100 bases:

>100_bases
CACTGAATATCCCAATCAATATTTGGGCAAAACTGGACAAGCGGCCGAGATTGAATAAAATCTGCGCTCTTTCTGTAATA
GCCCATAAAAAGGTACCCGA

Downstream 100 bases:

>100_bases
CCGCATAAAAAACGAGCCAGATGGCTCGTTTTTTGTTTAGCGTGGCTTACCGATGTAACCGAGCTTACGCCCAATCCAAA
AGATCAGGAGCAGTACTATG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MATLDVNPELYQAQLADKIARLKAMFVDYSMPELEVFESPVANYRMRAEFRIWHEGDDMYYIMFNQETREKYRVDQFPAA
SRLINDLMPLLMDAMKGSPILRHKLFQVDFLSTLSGEILVSLLYHRQLSEEWITAAQALKQRLNDEGFNLNLIGRARKMK
VVLDRDYVVENLQVNGQPYVYKQVENSFTQPNAKVAEKMLEWAVDCTQESKGDLLELYCGNGNFSLALAQNFERVLATEL
AKPSVEAAQFNIAANQIGNVQIIRMSAEEFTQAMEGKREFNRLKDAGVDLQSYRCNTIFVDPPRSGMDIDTCKMVQGYER
ILYISCNPETLQENLQVLGETHQVVRFALFDQFPYTHHMEAGVMLERKK

Sequences:

>Translated_369_residues
MATLDVNPELYQAQLADKIARLKAMFVDYSMPELEVFESPVANYRMRAEFRIWHEGDDMYYIMFNQETREKYRVDQFPAA
SRLINDLMPLLMDAMKGSPILRHKLFQVDFLSTLSGEILVSLLYHRQLSEEWITAAQALKQRLNDEGFNLNLIGRARKMK
VVLDRDYVVENLQVNGQPYVYKQVENSFTQPNAKVAEKMLEWAVDCTQESKGDLLELYCGNGNFSLALAQNFERVLATEL
AKPSVEAAQFNIAANQIGNVQIIRMSAEEFTQAMEGKREFNRLKDAGVDLQSYRCNTIFVDPPRSGMDIDTCKMVQGYER
ILYISCNPETLQENLQVLGETHQVVRFALFDQFPYTHHMEAGVMLERKK
>Mature_368_residues
ATLDVNPELYQAQLADKIARLKAMFVDYSMPELEVFESPVANYRMRAEFRIWHEGDDMYYIMFNQETREKYRVDQFPAAS
RLINDLMPLLMDAMKGSPILRHKLFQVDFLSTLSGEILVSLLYHRQLSEEWITAAQALKQRLNDEGFNLNLIGRARKMKV
VLDRDYVVENLQVNGQPYVYKQVENSFTQPNAKVAEKMLEWAVDCTQESKGDLLELYCGNGNFSLALAQNFERVLATELA
KPSVEAAQFNIAANQIGNVQIIRMSAEEFTQAMEGKREFNRLKDAGVDLQSYRCNTIFVDPPRSGMDIDTCKMVQGYERI
LYISCNPETLQENLQVLGETHQVVRFALFDQFPYTHHMEAGVMLERKK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=201, Percent_Identity=27.363184079602, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI51173878, Length=201, Percent_Identity=27.363184079602, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1790403, Length=360, Percent_Identity=64.7222222222222, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1789148, Length=301, Percent_Identity=26.9102990033223, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24666579, Length=438, Percent_Identity=23.2876712328767, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_VIBC3 (A5F4M4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4M4
- SMR:   A5F4M4
- STRING:   A5F4M4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2367
- eggNOG:   COG2265
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- ProtClustDB:   PRK05031
- BioCyc:   VCHO345073:VC0395_A2367-MONOMER
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 42578; Mature: 42447

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 326-326 BINDING 190-190 BINDING 218-218 BINDING 223-223 BINDING 239-239 BINDING 301-301

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLDVNPELYQAQLADKIARLKAMFVDYSMPELEVFESPVANYRMRAEFRIWHEGDDMY
CCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHCCEEEEEEEEEECCCCEE
YIMFNQETREKYRVDQFPAASRLINDLMPLLMDAMKGSPILRHKLFQVDFLSTLSGEILV
EEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLSEEWITAAQALKQRLNDEGFNLNLIGRARKMKVVLDRDYVVENLQVNGQPYV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCCCEEECEEECCCCEE
YKQVENSFTQPNAKVAEKMLEWAVDCTQESKGDLLELYCGNGNFSLALAQNFERVLATEL
EEHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHH
AKPSVEAAQFNIAANQIGNVQIIRMSAEEFTQAMEGKREFNRLKDAGVDLQSYRCNTIFV
CCCCCHHHEEEEEHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHEECCEEEE
DPPRSGMDIDTCKMVQGYERILYISCNPETLQENLQVLGETHQVVRFALFDQFPYTHHME
CCCCCCCCCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AGVMLERKK
CCEEEECCC
>Mature Secondary Structure 
ATLDVNPELYQAQLADKIARLKAMFVDYSMPELEVFESPVANYRMRAEFRIWHEGDDMY
CEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHCCEEEEEEEEEECCCCEE
YIMFNQETREKYRVDQFPAASRLINDLMPLLMDAMKGSPILRHKLFQVDFLSTLSGEILV
EEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLSEEWITAAQALKQRLNDEGFNLNLIGRARKMKVVLDRDYVVENLQVNGQPYV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCCCEEECEEECCCCEE
YKQVENSFTQPNAKVAEKMLEWAVDCTQESKGDLLELYCGNGNFSLALAQNFERVLATEL
EEHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHH
AKPSVEAAQFNIAANQIGNVQIIRMSAEEFTQAMEGKREFNRLKDAGVDLQSYRCNTIFV
CCCCCHHHEEEEEHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHEECCEEEE
DPPRSGMDIDTCKMVQGYERILYISCNPETLQENLQVLGETHQVVRFALFDQFPYTHHME
CCCCCCCCCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AGVMLERKK
CCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA