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The map label for this gene is dude

Identifier: 227080386

GI number: 227080386

Start: 141037

End: 142437

Strand: Reverse

Name: dude

Synonym: VCM66_0151

Alternate gene names: 227080386

Gene position: 142437-141037 (Counterclockwise)

Preceding gene: 227080389

Following gene: 227080381

Centisome position: 4.92

GC content: 49.32

Gene sequence:

>1401_bases
ATGGGACAGAAAAACCACTTTGACGTCATTGTGATCGGTAGCGGTCCGGGCGGAGAAGGGGCAGCCATGGGGCTGACCAA
AGGGGGAAAAAATGTCGCTATCATAGAAAAAGAGAGCAGTGTAGGCGGAGGTTGTACTCATTGGGGCACCATCCCATCGA
AAGCCCTACGTCACGCGGTAAGCCGCATTATTGAGTTCAATAGCAACCCGCTGTTTTGCAAAAACAACTCCAGCATTCAC
GCTACGTTTTCGACCATTTTAAGTCACGCCAAAAGCGTGATTGATAAACAAACACGCCTGCGCCAAGGCTTTTACGATCG
CAATCAATGCACGCTGATTTTTGGCGCTGCGCACTTTATTGATGCTCACACCGTAGCAGTCAAAAAAGCCGATGGCAGCA
TCGACACCTACAGTGCCGATAAATTTGTTATCGCCACCGGTTCACGCCCTTATCATCCGAAAGATGTCGATTTTGGTCAC
CCGCGTATTTATGACAGCGACTCGATTCTGAACCTCGAACATGATCCTCGCCATATCATTATTTACGGTGCTGGGGTGAT
TGGTTGTGAATACGCCTCTATCTTCCGCGGCTTGGATGTCAAAACGGATCTGATCAACACCCGTGATCGCTTACTGTCGT
TTCTGGATAATGAAGTCTCTGATGCGCTCTCTTACCACTTTTGGAACAGTGGTGTGGTGATCCGCAATGATGAAACTTAC
GACAAGGTGGAAGGCACCAGCGATGGGGTGATCGTCCATCTGAAATCGGGCAAAAAAATGCGCGCCGATTGCTTGTTGTA
CGCCAACGGGCGCACTGGTAATACTGATAAGCTCAATTTAGAGTCGGTTGGCTTACAAGCCGATTCGCGAGGCCAGTTAG
TCGTCAACGCCAATTACCAGACTCAAGTGGAACACATTTATGCGGTGGGTGATGTCATTGGTTACCCAAGCCTTGCCAGC
GCCGCTTATGACCAAGGTCGGTTTGTCGCGCAAGCCATCATTCATGGCCAAGCGGCACATTTGTTGACTGAAGATATCCC
AACCGGTATCTATACCATCCCTGAGATCAGCTCCGTGGGTCGTACCGAGCAAGAGCTGACGGCGGCTAAAGTGCCTTATG
AAGTGGGACGCGCTTCTTTCAAACATTTGGCGCGCGCGCAAATTGCGGGCAAAGATATCGGCAGCTTAAAAATCCTGTTC
CATCGCGAAACCAAAGAGATTTTAGGTATTCACTGCTTTGGTGAGCGTGCCGCAGAAATCATTCATATCGGCCAAGCGAT
CATGGAACAAAAAGGGGAAGCCAATACCATTGAGTATTTCGTGAACACCACCTTTAACTACCCGACGATGGCCGAAGCCT
TCCGTGTTGCAGCCTTAAATGGTCTTAATCGACTGTTTTAA

Upstream 100 bases:

>100_bases
GATCCCCTCCACTCTCTGCACCTTGCAGCATGTTTCCAGGTCAGGGAATGCGTTAAAATCTCGCAACAGCAATGTTAAGC
AAATATAACAAGGATTCACT

Downstream 100 bases:

>100_bases
TACGCTAGAGTCAAACTACTTAGAGTTGCAGGTAGACGGCAAGTGAGTTCATCCCCATGTGCATAGATACACTATGTGAT
TGGGGTGAACGAACGTAGCC

Product: soluble pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: STH; NAD(P)(+) transhydrogenase [B-specific]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MGQKNHFDVIVIGSGPGGEGAAMGLTKGGKNVAIIEKESSVGGGCTHWGTIPSKALRHAVSRIIEFNSNPLFCKNNSSIH
ATFSTILSHAKSVIDKQTRLRQGFYDRNQCTLIFGAAHFIDAHTVAVKKADGSIDTYSADKFVIATGSRPYHPKDVDFGH
PRIYDSDSILNLEHDPRHIIIYGAGVIGCEYASIFRGLDVKTDLINTRDRLLSFLDNEVSDALSYHFWNSGVVIRNDETY
DKVEGTSDGVIVHLKSGKKMRADCLLYANGRTGNTDKLNLESVGLQADSRGQLVVNANYQTQVEHIYAVGDVIGYPSLAS
AAYDQGRFVAQAIIHGQAAHLLTEDIPTGIYTIPEISSVGRTEQELTAAKVPYEVGRASFKHLARAQIAGKDIGSLKILF
HRETKEILGIHCFGERAAEIIHIGQAIMEQKGEANTIEYFVNTTFNYPTMAEAFRVAALNGLNRLF

Sequences:

>Translated_466_residues
MGQKNHFDVIVIGSGPGGEGAAMGLTKGGKNVAIIEKESSVGGGCTHWGTIPSKALRHAVSRIIEFNSNPLFCKNNSSIH
ATFSTILSHAKSVIDKQTRLRQGFYDRNQCTLIFGAAHFIDAHTVAVKKADGSIDTYSADKFVIATGSRPYHPKDVDFGH
PRIYDSDSILNLEHDPRHIIIYGAGVIGCEYASIFRGLDVKTDLINTRDRLLSFLDNEVSDALSYHFWNSGVVIRNDETY
DKVEGTSDGVIVHLKSGKKMRADCLLYANGRTGNTDKLNLESVGLQADSRGQLVVNANYQTQVEHIYAVGDVIGYPSLAS
AAYDQGRFVAQAIIHGQAAHLLTEDIPTGIYTIPEISSVGRTEQELTAAKVPYEVGRASFKHLARAQIAGKDIGSLKILF
HRETKEILGIHCFGERAAEIIHIGQAIMEQKGEANTIEYFVNTTFNYPTMAEAFRVAALNGLNRLF
>Mature_465_residues
GQKNHFDVIVIGSGPGGEGAAMGLTKGGKNVAIIEKESSVGGGCTHWGTIPSKALRHAVSRIIEFNSNPLFCKNNSSIHA
TFSTILSHAKSVIDKQTRLRQGFYDRNQCTLIFGAAHFIDAHTVAVKKADGSIDTYSADKFVIATGSRPYHPKDVDFGHP
RIYDSDSILNLEHDPRHIIIYGAGVIGCEYASIFRGLDVKTDLINTRDRLLSFLDNEVSDALSYHFWNSGVVIRNDETYD
KVEGTSDGVIVHLKSGKKMRADCLLYANGRTGNTDKLNLESVGLQADSRGQLVVNANYQTQVEHIYAVGDVIGYPSLASA
AYDQGRFVAQAIIHGQAAHLLTEDIPTGIYTIPEISSVGRTEQELTAAKVPYEVGRASFKHLARAQIAGKDIGSLKILFH
RETKEILGIHCFGERAAEIIHIGQAIMEQKGEANTIEYFVNTTFNYPTMAEAFRVAALNGLNRLF

Specific function: Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=471, Percent_Identity=29.2993630573248, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI50301238, Length=461, Percent_Identity=27.5488069414317, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI22035672, Length=469, Percent_Identity=26.6524520255864, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI33519430, Length=324, Percent_Identity=25.3086419753086, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI33519428, Length=324, Percent_Identity=25.3086419753086, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI33519426, Length=324, Percent_Identity=25.3086419753086, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI148277071, Length=324, Percent_Identity=25.3086419753086, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI148277065, Length=406, Percent_Identity=23.6453201970443, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=23.448275862069, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI291045268, Length=309, Percent_Identity=24.2718446601942, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=74.892703862661, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI1786307, Length=461, Percent_Identity=29.0672451193059, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=29.3577981651376, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=25.9911894273128, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=30.4721030042918, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=24.416135881104, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71983429, Length=363, Percent_Identity=27.5482093663912, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71983419, Length=363, Percent_Identity=27.5482093663912, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71982272, Length=501, Percent_Identity=24.9500998003992, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=258, Percent_Identity=24.8062015503876, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=485, Percent_Identity=27.6288659793814, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=27.3504273504274, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=27.3684210526316, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21358499, Length=458, Percent_Identity=32.3144104803493, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24640549, Length=487, Percent_Identity=27.7207392197125, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=27.7207392197125, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=27.7207392197125, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=25.0513347022587, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): STHA_VIBC3 (A5F4K5)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4K5
- SMR:   A5F4K5
- STRING:   A5F4K5
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2370
- eggNOG:   COG1249
- HOGENOM:   HBG515043
- OMA:   NVKVDLI
- ProtClustDB:   PRK05249
- BioCyc:   VCHO345073:VC0395_A2370-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00247
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327
- InterPro:   IPR022962
- Gene3D:   G3DSA:3.30.390.30
- PRINTS:   PR00368

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.6.1.1

Molecular weight: Translated: 50942; Mature: 50811

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQKNHFDVIVIGSGPGGEGAAMGLTKGGKNVAIIEKESSVGGGCTHWGTIPSKALRHAV
CCCCCCEEEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHH
SRIIEFNSNPLFCKNNSSIHATFSTILSHAKSVIDKQTRLRQGFYDRNQCTLIFGAAHFI
HHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHH
DAHTVAVKKADGSIDTYSADKFVIATGSRPYHPKDVDFGHPRIYDSDSILNLEHDPRHII
CCEEEEEEECCCCCEEECCCEEEEEECCCCCCCCCCCCCCCEEECCCCEEEECCCCCEEE
IYGAGVIGCEYASIFRGLDVKTDLINTRDRLLSFLDNEVSDALSYHFWNSGVVIRNDETY
EEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCH
DKVEGTSDGVIVHLKSGKKMRADCLLYANGRTGNTDKLNLESVGLQADSRGQLVVNANYQ
HHCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCEEEHHHCCCCCCCCCCEEEECCCC
TQVEHIYAVGDVIGYPSLASAAYDQGRFVAQAIIHGQAAHLLTEDIPTGIYTIPEISSVG
HHHHHEEEHHHHHCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCEEECCCHHHCC
RTEQELTAAKVPYEVGRASFKHLARAQIAGKDIGSLKILFHRETKEILGIHCFGERAAEI
CCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHEEEEEECHHHHHH
IHIGQAIMEQKGEANTIEYFVNTTFNYPTMAEAFRVAALNGLNRLF
HHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GQKNHFDVIVIGSGPGGEGAAMGLTKGGKNVAIIEKESSVGGGCTHWGTIPSKALRHAV
CCCCCEEEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHH
SRIIEFNSNPLFCKNNSSIHATFSTILSHAKSVIDKQTRLRQGFYDRNQCTLIFGAAHFI
HHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHH
DAHTVAVKKADGSIDTYSADKFVIATGSRPYHPKDVDFGHPRIYDSDSILNLEHDPRHII
CCEEEEEEECCCCCEEECCCEEEEEECCCCCCCCCCCCCCCEEECCCCEEEECCCCCEEE
IYGAGVIGCEYASIFRGLDVKTDLINTRDRLLSFLDNEVSDALSYHFWNSGVVIRNDETY
EEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCH
DKVEGTSDGVIVHLKSGKKMRADCLLYANGRTGNTDKLNLESVGLQADSRGQLVVNANYQ
HHCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCEEEHHHCCCCCCCCCCEEEECCCC
TQVEHIYAVGDVIGYPSLASAAYDQGRFVAQAIIHGQAAHLLTEDIPTGIYTIPEISSVG
HHHHHEEEHHHHHCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCEEECCCHHHCC
RTEQELTAAKVPYEVGRASFKHLARAQIAGKDIGSLKILFHRETKEILGIHCFGERAAEI
CCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHEEEEEECHHHHHH
IHIGQAIMEQKGEANTIEYFVNTTFNYPTMAEAFRVAALNGLNRLF
HHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA