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The map label for this gene is rsmD [H]

Identifier: 227080381

GI number: 227080381

Start: 136116

End: 136715

Strand: Reverse

Name: rsmD [H]

Synonym: VCM66_0146

Alternate gene names: 227080381

Gene position: 136715-136116 (Counterclockwise)

Preceding gene: 227080386

Following gene: 227080380

Centisome position: 4.73

GC content: 49.83

Gene sequence:

>600_bases
ATGCAAAGACGTCGCCAGCCAAACCCATCACAAAAAAAGGCCCCTACAGGGCAAGTTCGGATTATCAGCGGTCTGTGGCG
CGGCCGAAAATTGCCCGTGCATGATGCCGAAGGACTGCGCCCGACGACCGATCGAGTGAAAGAAACGCTGTTTAACTGGC
TTGCTCAAGAAGTGCCAAATGCTCGCTGTCTCGATCTTTTTGCCGGTTCTGGCGGGCTAGGCTTCGAGTGTGCATCACGC
CAAGCCAACCAAGTAACCATGCTTGAGCTGAACCCGCTCGCCTTTCAGCAACTCAAAACTAATGTACAGAGCCTGAAAGC
AGAAAATATTGAGCTTATTCACACGGATGCACTCGCCTTTCTGCGACAATCGGGTAAACCTTATGACGTGGTGTTTATTG
ACCCACCTTTTCGACAAGGTTTATTACAGGAAACCACTCAGTTACTCGAGCAAAACGGTTGGCTCGCGCCACAAGCGATG
ATTTATATTGAGTGCGAAAAAGAGCTGCCACTGACGGAGTTGCCTCCTCATTGGCATCTACATCGAGAAAAGAGTGCCGG
ACAAGTCTGTTATCGGCTGTTTATAAGGGAAGAACAATGA

Upstream 100 bases:

>100_bases
CTTGGTACACTTTGCCCAGTTTTTTATGTTAACTCGGGTTGTCACGCCAAATGGCTGACAAGCGAGGTATAGTAACACTT
AATTAGCGGTCAAAAAATCT

Downstream 100 bases:

>100_bases
AAGCACTACTCTGGTTAGCGAAAGCGGCGATTGGTTTTGTATGGCTGGTTTTGCTGTTCAACATCGTGATGCCTTTTCCG
GGTAATGCCGCCATCGCACT

Product: Putative methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MQRRRQPNPSQKKAPTGQVRIISGLWRGRKLPVHDAEGLRPTTDRVKETLFNWLAQEVPNARCLDLFAGSGGLGFECASR
QANQVTMLELNPLAFQQLKTNVQSLKAENIELIHTDALAFLRQSGKPYDVVFIDPPFRQGLLQETTQLLEQNGWLAPQAM
IYIECEKELPLTELPPHWHLHREKSAGQVCYRLFIREEQ

Sequences:

>Translated_199_residues
MQRRRQPNPSQKKAPTGQVRIISGLWRGRKLPVHDAEGLRPTTDRVKETLFNWLAQEVPNARCLDLFAGSGGLGFECASR
QANQVTMLELNPLAFQQLKTNVQSLKAENIELIHTDALAFLRQSGKPYDVVFIDPPFRQGLLQETTQLLEQNGWLAPQAM
IYIECEKELPLTELPPHWHLHREKSAGQVCYRLFIREEQ
>Mature_199_residues
MQRRRQPNPSQKKAPTGQVRIISGLWRGRKLPVHDAEGLRPTTDRVKETLFNWLAQEVPNARCLDLFAGSGGLGFECASR
QANQVTMLELNPLAFQQLKTNVQSLKAENIELIHTDALAFLRQSGKPYDVVFIDPPFRQGLLQETTQLLEQNGWLAPQAM
IYIECEKELPLTELPPHWHLHREKSAGQVCYRLFIREEQ

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=195, Percent_Identity=56.4102564102564, Blast_Score=231, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22793; Mature: 22793

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRRQPNPSQKKAPTGQVRIISGLWRGRKLPVHDAEGLRPTTDRVKETLFNWLAQEVPN
CCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
ARCLDLFAGSGGLGFECASRQANQVTMLELNPLAFQQLKTNVQSLKAENIELIHTDALAF
CEEEEEEECCCCCCCHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCEEEEEHHHHHH
LRQSGKPYDVVFIDPPFRQGLLQETTQLLEQNGWLAPQAMIYIECEKELPLTELPPHWHL
HHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCC
HREKSAGQVCYRLFIREEQ
CCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQRRRQPNPSQKKAPTGQVRIISGLWRGRKLPVHDAEGLRPTTDRVKETLFNWLAQEVPN
CCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
ARCLDLFAGSGGLGFECASRQANQVTMLELNPLAFQQLKTNVQSLKAENIELIHTDALAF
CEEEEEEECCCCCCCHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCEEEEEHHHHHH
LRQSGKPYDVVFIDPPFRQGLLQETTQLLEQNGWLAPQAMIYIECEKELPLTELPPHWHL
HHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCC
HREKSAGQVCYRLFIREEQ
CCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]