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The map label for this gene is gidB [H]

Identifier: 226951093

GI number: 226951093

Start: 4148765

End: 4149484

Strand: Reverse

Name: gidB [H]

Synonym: CLM_4146

Alternate gene names: 226951093

Gene position: 4149484-4148765 (Counterclockwise)

Preceding gene: 226951094

Following gene: 226951092

Centisome position: 99.86

GC content: 27.08

Gene sequence:

>720_bases
ATGGAATTTTTCAACATACTACAATCAGCATGCAATGATGTTAATTTAGATTTTAATGATAAAAAATATAATCAGCTTAT
AAGTTATAAAAATTTAATACAGGAATGGAATAAGAAAATAAATCTAACAGCTATTGTTGAAGATGATGAAATAATAAAAA
AACATTTTATAGATTGTATAAAAATATTTAAATCTTCACCTATAGGAGAAGCTAAAAGTTTAATAGATATAGGTACAGGT
GCAGGATTTCCAGGCATCCCCATAAAAATATTAAAAGAAGACATAGAAATAACTTTATTGGATTCCCTACAAAAGAGAAT
AAATTTTTTAAATATTGTGATAGGAGAGCTACAATTAAAAAATATACAATGTTTACATGGAAGAGCAGAAGATTATGCAC
AGGAAATTCAGCATAGACAAAAATATGATATAGCAGTTTCCCGAGCAGTAGCGAATTTAGCAGTTTTAAGTGAATTTTGT
ATTCCTTTTGTAGAAAAAGGTGGATATTTTATAGCCATGAAAGGTCCGTCTGTAGAAGAGGAAATAACAGCAGCTACAAA
ATCTATAGAAATTTTAGGTGGAAAAATAGAGGATATAATGAAAATAGATATTGAAGATACAGATCTAAAACATAATCTAG
TAATTATAAAAAAATTAAGAGAAACGGGAAAAAGGTATCCTAGAAAGCCCGGTATTATTAAAAAAAATCCTCTAAAATAA

Upstream 100 bases:

>100_bases
CTAGAATTTCAGGTGTATCTCCAGCAGATATATCAGTATTACTTATATATATGGAAAGAAAAAATAGAGAAAACTAGAAA
ATCCTTTTGGAGGTTTTTAA

Downstream 100 bases:

>100_bases
ATGTAATAATTTGCAATTAAAAAAATAATAAAAAAAGAAGGAAAATTACATTTAAAGAAGAAATGTAAATATTAGGAAGA
GAATAAGAGGGATGGTAGTA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCIKIFKSSPIGEAKSLIDIGTG
AGFPGIPIKILKEDIEITLLDSLQKRINFLNIVIGELQLKNIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFC
IPFVEKGGYFIAMKGPSVEEEITAATKSIEILGGKIEDIMKIDIEDTDLKHNLVIIKKLRETGKRYPRKPGIIKKNPLK

Sequences:

>Translated_239_residues
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCIKIFKSSPIGEAKSLIDIGTG
AGFPGIPIKILKEDIEITLLDSLQKRINFLNIVIGELQLKNIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFC
IPFVEKGGYFIAMKGPSVEEEITAATKSIEILGGKIEDIMKIDIEDTDLKHNLVIIKKLRETGKRYPRKPGIIKKNPLK
>Mature_239_residues
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCIKIFKSSPIGEAKSLIDIGTG
AGFPGIPIKILKEDIEITLLDSLQKRINFLNIVIGELQLKNIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFC
IPFVEKGGYFIAMKGPSVEEEITAATKSIEILGGKIEDIMKIDIEDTDLKHNLVIIKKLRETGKRYPRKPGIIKKNPLK

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=215, Percent_Identity=33.953488372093, Blast_Score=137, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 27156; Mature: 27156

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCI
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHH
KIFKSSPIGEAKSLIDIGTGAGFPGIPIKILKEDIEITLLDSLQKRINFLNIVIGELQLK
HHHHCCCCCHHHHHHEECCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEC
NIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFCIPFVEKGGYFIAMKGPSVEE
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHH
EITAATKSIEILGGKIEDIMKIDIEDTDLKHNLVIIKKLRETGKRYPRKPGIIKKNPLK
HHHHHHHHHHHHCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCI
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHH
KIFKSSPIGEAKSLIDIGTGAGFPGIPIKILKEDIEITLLDSLQKRINFLNIVIGELQLK
HHHHCCCCCHHHHHHEECCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEC
NIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFCIPFVEKGGYFIAMKGPSVEE
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHH
EITAATKSIEILGGKIEDIMKIDIEDTDLKHNLVIIKKLRETGKRYPRKPGIIKKNPLK
HHHHHHHHHHHHCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA