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The map label for this gene is yeiE [H]

Identifier: 226951045

GI number: 226951045

Start: 4104456

End: 4105337

Strand: Reverse

Name: yeiE [H]

Synonym: CLM_4084

Alternate gene names: 226951045

Gene position: 4105337-4104456 (Counterclockwise)

Preceding gene: 226951046

Following gene: 226951040

Centisome position: 98.8

GC content: 25.96

Gene sequence:

>882_bases
ATGAATGAAAGAAAGCTTAGGATATTTTATGAAGTAGCAGAAAAACTTAATATGACAGAAGTGGCAGAAAAGCTATATAT
AAGTCAGCCAGCAGTTAGTCAAACTATATTAGAATTAGAGAATGAATTAGGGGTTAAATTATTTGATAGAATAAATAGAA
GGTTATATTTAACAAATGAAGGGGAGCTTTTTTTAAATTATGTGAGAAGAATTTTAAATATGTATGATGATGCTATAAGA
AGTCTTAAGGATATTAATAGCTTGAAAACAGGAAAGCTTAAAATAGGAGCTAGTACTACTATAGGAATATATATTTTACC
AGATATAATAGGCAAATTTACAAAGCAGTATAAAGGAATAGATACATCTATAGCTATAGAAAATACTAAATTGATAAGTG
ATATGATATTAGATAACAGTATTGATTTTGCATTTGTTGAGGGGCCTGTATATAATGAGGAAATTATAAAAGAAAACTTT
TGTGATGATGAATTAGTTTTTATAACTAGTCCAGAACATCCCTGGAGTAATAAGGATGAGATAAGTATAAATGAAGTAGA
TAGAGAAAAGATAATAATGAGAGAAATAGGAAGTGGTACAAGAGAAGTTTTTGAAAGTTATATGACCGCTAATAATGTAA
ATTATAATATAGCCTTTGAATTAGGAAATACGGAAGCTATTAAAAAGGCTGTTGAAGCGGGATTAGGAGTATCATGTATA
TCTAAAAGATGTATTAAAAATGAAATAAATAATAATACATTATCATGGACAAAGTTAAAAGAATTTGAAATAAAAAGAAA
ACTTATACTTATATATCATAAAGATAAGTTTTTATCTACATTATTAAAAACCTTTATAAAGTTTGCATTAAAAAATGTAT
AA

Upstream 100 bases:

>100_bases
TACGAAAACTTAAAAAATTATAAAGAGGGAACGGAATTAAAAAGCGTTGTTAATATGAATAAAGGTTATTAAAATTAAAG
GTATAATTTAAGGTGATTTT

Downstream 100 bases:

>100_bases
AAAAAAGAGCATAGGCTCTTTTTTTATTATATATTTTAAGGTAAAAGTTTATTTATATCTATAATTCCAGCACCTTGAAT
CCACTTAGAAAAAGGTAAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNERKLRIFYEVAEKLNMTEVAEKLYISQPAVSQTILELENELGVKLFDRINRRLYLTNEGELFLNYVRRILNMYDDAIR
SLKDINSLKTGKLKIGASTTIGIYILPDIIGKFTKQYKGIDTSIAIENTKLISDMILDNSIDFAFVEGPVYNEEIIKENF
CDDELVFITSPEHPWSNKDEISINEVDREKIIMREIGSGTREVFESYMTANNVNYNIAFELGNTEAIKKAVEAGLGVSCI
SKRCIKNEINNNTLSWTKLKEFEIKRKLILIYHKDKFLSTLLKTFIKFALKNV

Sequences:

>Translated_293_residues
MNERKLRIFYEVAEKLNMTEVAEKLYISQPAVSQTILELENELGVKLFDRINRRLYLTNEGELFLNYVRRILNMYDDAIR
SLKDINSLKTGKLKIGASTTIGIYILPDIIGKFTKQYKGIDTSIAIENTKLISDMILDNSIDFAFVEGPVYNEEIIKENF
CDDELVFITSPEHPWSNKDEISINEVDREKIIMREIGSGTREVFESYMTANNVNYNIAFELGNTEAIKKAVEAGLGVSCI
SKRCIKNEINNNTLSWTKLKEFEIKRKLILIYHKDKFLSTLLKTFIKFALKNV
>Mature_293_residues
MNERKLRIFYEVAEKLNMTEVAEKLYISQPAVSQTILELENELGVKLFDRINRRLYLTNEGELFLNYVRRILNMYDDAIR
SLKDINSLKTGKLKIGASTTIGIYILPDIIGKFTKQYKGIDTSIAIENTKLISDMILDNSIDFAFVEGPVYNEEIIKENF
CDDELVFITSPEHPWSNKDEISINEVDREKIIMREIGSGTREVFESYMTANNVNYNIAFELGNTEAIKKAVEAGLGVSCI
SKRCIKNEINNNTLSWTKLKEFEIKRKLILIYHKDKFLSTLLKTFIKFALKNV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=285, Percent_Identity=34.7368421052632, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI157672245, Length=290, Percent_Identity=25.1724137931034, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787879, Length=285, Percent_Identity=26.6666666666667, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788748, Length=285, Percent_Identity=25.2631578947368, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790399, Length=294, Percent_Identity=23.8095238095238, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI145693105, Length=285, Percent_Identity=27.3684210526316, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=26.5060240963855, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788887, Length=248, Percent_Identity=31.0483870967742, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082132, Length=287, Percent_Identity=23.6933797909408, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081904, Length=205, Percent_Identity=29.7560975609756, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787806, Length=261, Percent_Identity=24.904214559387, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789204, Length=243, Percent_Identity=24.2798353909465, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=25, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788296, Length=245, Percent_Identity=25.3061224489796, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=24.8031496062992, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787601, Length=175, Percent_Identity=27.4285714285714, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789639, Length=266, Percent_Identity=24.4360902255639, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788626, Length=234, Percent_Identity=26.4957264957265, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788297, Length=255, Percent_Identity=21.9607843137255, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367136, Length=116, Percent_Identity=31.0344827586207, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788706, Length=169, Percent_Identity=27.2189349112426, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790783, Length=248, Percent_Identity=22.9838709677419, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790011, Length=288, Percent_Identity=23.2638888888889, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33809; Mature: 33809

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNERKLRIFYEVAEKLNMTEVAEKLYISQPAVSQTILELENELGVKLFDRINRRLYLTNE
CCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCEEEEECC
GELFLNYVRRILNMYDDAIRSLKDINSLKTGKLKIGASTTIGIYILPDIIGKFTKQYKGI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHHCCC
DTSIAIENTKLISDMILDNSIDFAFVEGPVYNEEIIKENFCDDELVFITSPEHPWSNKDE
CCEEEECHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCC
ISINEVDREKIIMREIGSGTREVFESYMTANNVNYNIAFELGNTEAIKKAVEAGLGVSCI
EEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCHHHH
SKRCIKNEINNNTLSWTKLKEFEIKRKLILIYHKDKFLSTLLKTFIKFALKNV
HHHHHHHHCCCCCEEEHHHHHHHHCEEEEEEEECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNERKLRIFYEVAEKLNMTEVAEKLYISQPAVSQTILELENELGVKLFDRINRRLYLTNE
CCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCEEEEECC
GELFLNYVRRILNMYDDAIRSLKDINSLKTGKLKIGASTTIGIYILPDIIGKFTKQYKGI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHHCCC
DTSIAIENTKLISDMILDNSIDFAFVEGPVYNEEIIKENFCDDELVFITSPEHPWSNKDE
CCEEEECHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCC
ISINEVDREKIIMREIGSGTREVFESYMTANNVNYNIAFELGNTEAIKKAVEAGLGVSCI
EEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCHHHH
SKRCIKNEINNNTLSWTKLKEFEIKRKLILIYHKDKFLSTLLKTFIKFALKNV
HHHHHHHHCCCCCEEEHHHHHHHHCEEEEEEEECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]