Click here to switch to the map view.

The map label for this gene is hup [H]

Identifier: 226950990

GI number: 226950990

Start: 4044928

End: 4045206

Strand: Reverse

Name: hup [H]

Synonym: CLM_4026

Alternate gene names: 226950990

Gene position: 4045206-4044928 (Counterclockwise)

Preceding gene: 226950991

Following gene: 226950989

Centisome position: 97.35

GC content: 31.54

Gene sequence:

>279_bases
GTGAATAAATCAGAATTAATTACAAGCATGGCAGAAAAATCAAAATTAACTAAAAAGGATGCAGAAACAGCTTTAAAGGC
ATTTATAGAAAGTGTTGAAGAAGCATTAGAAGGTGGAGAAAAAGTTCAATTAGTTGGCTTTGGTACTTTTGAAACTAGGG
AAAGAGCTGAAAGAGTAGGTAGAAATCCAAGAACTAAGGAAGAAATAACTATACCTGCATCAATAGCACCTGTTTTCAAA
GCAGGAAAAGAACTAAAAGAAAAAGTAAACAAAAAATAA

Upstream 100 bases:

>100_bases
TTAAAGAAGGATTTTTATAATAAGTGAAGAATAAGCTAGTATGCGAATAATGTATAAAACTTTAAGTAGGCAACTACTTA
ATTTTAAGGAGGTAACAAAA

Downstream 100 bases:

>100_bases
ATAATAAATATAAAAAACCTAGGTTTTAATCTGGGTTTTTTGTTTATTTATATAAAATTAAGGGGGAAAGGATTTGCGTT
TAGATAAATTTCTTAAAGTT

Product: DNA-binding protein HU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 92; Mature: 92

Protein sequence:

>92_residues
MNKSELITSMAEKSKLTKKDAETALKAFIESVEEALEGGEKVQLVGFGTFETRERAERVGRNPRTKEEITIPASIAPVFK
AGKELKEKVNKK

Sequences:

>Translated_92_residues
MNKSELITSMAEKSKLTKKDAETALKAFIESVEEALEGGEKVQLVGFGTFETRERAERVGRNPRTKEEITIPASIAPVFK
AGKELKEKVNKK
>Mature_92_residues
MNKSELITSMAEKSKLTKKDAETALKAFIESVEEALEGGEKVQLVGFGTFETRERAERVGRNPRTKEEITIPASIAPVFK
AGKELKEKVNKK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=54.4444444444444, Blast_Score=97, Evalue=1e-22,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=50.561797752809, Blast_Score=93, Evalue=3e-21,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=40.4494382022472, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=39.5604395604396, Blast_Score=74, Evalue=2e-15,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 10223; Mature: 10223

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSELITSMAEKSKLTKKDAETALKAFIESVEEALEGGEKVQLVGFGTFETRERAERVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHC
RNPRTKEEITIPASIAPVFKAGKELKEKVNKK
CCCCCCCCEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKSELITSMAEKSKLTKKDAETALKAFIESVEEALEGGEKVQLVGFGTFETRERAERVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHC
RNPRTKEEITIPASIAPVFKAGKELKEKVNKK
CCCCCCCCEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6540370 [H]