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The map label for this gene is dus [H]

Identifier: 226950978

GI number: 226950978

Start: 4031680

End: 4032651

Strand: Reverse

Name: dus [H]

Synonym: CLM_4007

Alternate gene names: 226950978

Gene position: 4032651-4031680 (Counterclockwise)

Preceding gene: 226950979

Following gene: 226950977

Centisome position: 97.05

GC content: 29.32

Gene sequence:

>972_bases
ATGAATATAGGAAATCTTATATTTCATAATAATGTATTTTTAGCACCTATGGCAGGATTTACAGATATAGCTTTTAGAGA
GATATGTAAAGAATTAGGATGTGGTTTGGTTTACACTGAAATGGTAAGTGCTAAAGCATTATATTATGAAAGTAATAATA
CAAAAGAATTATGTGTAATATCAAATAAAGAAAAACCAGTAGCACTGCAATTATTTGGTCATGAACCAGAGATAATGGCA
AATGCAGTAGAATTTTTTAATAATAATGATGATGTATGTATATTAGATGTAAATATGGGATGTCCAGCACCTAAAATAGT
TAAAAATGGTGATGGTTCTGCACTTATGAAAGATCCTAAATTAGCATCAGAAATAATAAAAGCTATGAAAAAGGTAGCTA
AAAAACCTATTACAGTTAAGTTTAGAAAAGGTTTTGACAAAAATAATATTAATGCGGTAGAGTTTGCTAAAATAATGGAA
CAATCTGGAGTTGATGCTATAACTATTCATGGTAGAACACGAGAGCAAATGTATGAAGGAAAGGCAGATTGGGCAATAAT
ATCAAAAGTAAAAAATTCTGTAAGCATACCTGTGATAGGCAATGGAGATGTATTTTCATCAGAAGATGCTTTAGAAATGA
TAAATAAAACAGATTGTGATGGTATAATGATTGGAAGGGGTGCACAAGGTAACCCATGGATATTTAAGCAAATAAATGAA
AAAATTAAGGGAGAACATGTATATTATCCTACTCCACAAGAAAGAATAGACATATGTATAAATCATTATAAAAAATCACT
AGAGTATTTTGAAGAGCATAAGGCAGTAAGAGAGATGAGAAAACATGTGGCAGTATATGTAAAAGGCCTTAAAAATTGCA
CTGATATAAAAGATAAAGTAAATATGGAGAAAGATCCTGATAAAGTGTTAGAAGAATTAATTAAGTATAGAGAAACATTA
AGGGAATTTTAA

Upstream 100 bases:

>100_bases
ATTAATTAGTGAAGAATCAAAAAGTATTGATGAAATAAATGCTATTTTAACCTTAGAAGGATTAAGAGTAATATATGAAA
AAAATAAATAAGGTGATTAT

Downstream 100 bases:

>100_bases
ATAAATATTTACATAGAAATATATTGTAATCAATTTTTATGGTGAAATAATGAAGCCAATAATAGATATAAGCTCTAATA
TAATGAAAGACTATAATAAA

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNIGNLIFHNNVFLAPMAGFTDIAFREICKELGCGLVYTEMVSAKALYYESNNTKELCVISNKEKPVALQLFGHEPEIMA
NAVEFFNNNDDVCILDVNMGCPAPKIVKNGDGSALMKDPKLASEIIKAMKKVAKKPITVKFRKGFDKNNINAVEFAKIME
QSGVDAITIHGRTREQMYEGKADWAIISKVKNSVSIPVIGNGDVFSSEDALEMINKTDCDGIMIGRGAQGNPWIFKQINE
KIKGEHVYYPTPQERIDICINHYKKSLEYFEEHKAVREMRKHVAVYVKGLKNCTDIKDKVNMEKDPDKVLEELIKYRETL
REF

Sequences:

>Translated_323_residues
MNIGNLIFHNNVFLAPMAGFTDIAFREICKELGCGLVYTEMVSAKALYYESNNTKELCVISNKEKPVALQLFGHEPEIMA
NAVEFFNNNDDVCILDVNMGCPAPKIVKNGDGSALMKDPKLASEIIKAMKKVAKKPITVKFRKGFDKNNINAVEFAKIME
QSGVDAITIHGRTREQMYEGKADWAIISKVKNSVSIPVIGNGDVFSSEDALEMINKTDCDGIMIGRGAQGNPWIFKQINE
KIKGEHVYYPTPQERIDICINHYKKSLEYFEEHKAVREMRKHVAVYVKGLKNCTDIKDKVNMEKDPDKVLEELIKYRETL
REF
>Mature_323_residues
MNIGNLIFHNNVFLAPMAGFTDIAFREICKELGCGLVYTEMVSAKALYYESNNTKELCVISNKEKPVALQLFGHEPEIMA
NAVEFFNNNDDVCILDVNMGCPAPKIVKNGDGSALMKDPKLASEIIKAMKKVAKKPITVKFRKGFDKNNINAVEFAKIME
QSGVDAITIHGRTREQMYEGKADWAIISKVKNSVSIPVIGNGDVFSSEDALEMINKTDCDGIMIGRGAQGNPWIFKQINE
KIKGEHVYYPTPQERIDICINHYKKSLEYFEEHKAVREMRKHVAVYVKGLKNCTDIKDKVNMEKDPDKVLEELIKYRETL
REF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=287, Percent_Identity=31.0104529616725, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI239788462, Length=288, Percent_Identity=30.9027777777778, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI40807366, Length=268, Percent_Identity=28.7313432835821, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI31742496, Length=244, Percent_Identity=28.2786885245902, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI8923374, Length=254, Percent_Identity=27.5590551181102, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1789660, Length=319, Percent_Identity=34.4827586206897, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788462, Length=321, Percent_Identity=29.2834890965732, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI145693211, Length=246, Percent_Identity=28.0487804878049, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=279, Percent_Identity=31.8996415770609, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17507177, Length=315, Percent_Identity=26.3492063492064, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=29.5964125560538, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=251, Percent_Identity=28.2868525896414, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=290, Percent_Identity=28.6206896551724, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323560, Length=297, Percent_Identity=26.9360269360269, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=27.3127753303965, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585320, Length=279, Percent_Identity=31.8996415770609, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24580595, Length=232, Percent_Identity=29.3103448275862, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19920448, Length=232, Percent_Identity=29.3103448275862, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19921524, Length=226, Percent_Identity=26.5486725663717, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45549423, Length=249, Percent_Identity=26.5060240963855, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36568; Mature: 36568

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGNLIFHNNVFLAPMAGFTDIAFREICKELGCGLVYTEMVSAKALYYESNNTKELCVI
CCCCCEEEECCEEEEECCCHHHHHHHHHHHHHCCCEEHHHHHHHHEEEEECCCCEEEEEE
SNKEKPVALQLFGHEPEIMANAVEFFNNNDDVCILDVNMGCPAPKIVKNGDGSALMKDPK
ECCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHEECCCCCCEECCHH
LASEIIKAMKKVAKKPITVKFRKGFDKNNINAVEFAKIMEQSGVDAITIHGRTREQMYEG
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHCC
KADWAIISKVKNSVSIPVIGNGDVFSSEDALEMINKTDCDGIMIGRGAQGNPWIFKQINE
CCCHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
KIKGEHVYYPTPQERIDICINHYKKSLEYFEEHKAVREMRKHVAVYVKGLKNCTDIKDKV
HHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NMEKDPDKVLEELIKYRETLREF
CCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIGNLIFHNNVFLAPMAGFTDIAFREICKELGCGLVYTEMVSAKALYYESNNTKELCVI
CCCCCEEEECCEEEEECCCHHHHHHHHHHHHHCCCEEHHHHHHHHEEEEECCCCEEEEEE
SNKEKPVALQLFGHEPEIMANAVEFFNNNDDVCILDVNMGCPAPKIVKNGDGSALMKDPK
ECCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHEECCCCCCEECCHH
LASEIIKAMKKVAKKPITVKFRKGFDKNNINAVEFAKIMEQSGVDAITIHGRTREQMYEG
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHCC
KADWAIISKVKNSVSIPVIGNGDVFSSEDALEMINKTDCDGIMIGRGAQGNPWIFKQINE
CCCHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
KIKGEHVYYPTPQERIDICINHYKKSLEYFEEHKAVREMRKHVAVYVKGLKNCTDIKDKV
HHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NMEKDPDKVLEELIKYRETLREF
CCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]