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The map label for this gene is yacO [H]

Identifier: 226950952

GI number: 226950952

Start: 3994533

End: 3995306

Strand: Reverse

Name: yacO [H]

Synonym: CLM_3969

Alternate gene names: 226950952

Gene position: 3995306-3994533 (Counterclockwise)

Preceding gene: 226950953

Following gene: 226950951

Centisome position: 96.15

GC content: 30.88

Gene sequence:

>774_bases
ATGAAAAATAGAGTTGGTCAACAGGAAGAAATAAGAGAAGATATAATAGAGGGCAGAAATGCGGTAATAGAAGCATTAAA
ATCAAATAAAACTATAGAAAAGGTAATGGTAGCAAAAGGAGATTTAGAAGGATCTATCAAAATCATAATATCTTTAGCTA
AGGAAAAAGGAATAGTTATAAATGAAGTTGATAGAAAAAAACTCGACTCTATATCCCAAACAAGAGCTCATCAGGGAGTT
ATAGCATTTACAACTCCATATCAATATTGTGCAGTAGAAGATATTATACGATACGCAAAACAAAAAGAAGAGGATCCCTT
TATAATAATATTAGATGAAATAGAAGATCCACACAATTTTGGATCCATATTAAGAACGGCAGAAGTTTGTGGAGCACATG
GTGTCATAATACCTAAGAGAAGAAATGTAGGAGTTACTCCTACTGTTTATAAAACATCTGCTGGAGCCGTAGAATATATG
AAAATAAGTAAGGTTACCAATATAAATAATGTTATAGATAAACTTAAAGAAAAGGGTATATGGATATATGGCGCAGATAT
GTGTGGAAATGATTATTGTTTTGATGTAAATCTATCTGGACCTATAGCATTAGTTATAGGAAGTGAAGGAAGAGGAATAT
CAAAGTTAACTAAAAATAAATGTGATGTTTTAGTTAAAATCCCAATGCTTGGGAATATAACATCTCTTAATGCATCTGTT
GCTGGAGGAATGTTAATGTATGAAATATTAAAACAAAGGATGAAGAGCAAATAA

Upstream 100 bases:

>100_bases
GCGTAAATGGACCTTGTCCAGAAGGAAAAATGTCCTGTGGTAAAATAAAAGAAGTTAGAGAAAAGTACAATGTAAAATTA
TACAAGGAGAGTAAGTAATA

Downstream 100 bases:

>100_bases
TTATGAAATATATATTTGTAGATGCTTATAATGTCATAAATAGTTGGAAAGAATTAAAAAAGATAAAAGACTATAATTTA
GAAATGTCAAGAGAGCAACT

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKNRVGQQEEIREDIIEGRNAVIEALKSNKTIEKVMVAKGDLEGSIKIIISLAKEKGIVINEVDRKKLDSISQTRAHQGV
IAFTTPYQYCAVEDIIRYAKQKEEDPFIIILDEIEDPHNFGSILRTAEVCGAHGVIIPKRRNVGVTPTVYKTSAGAVEYM
KISKVTNINNVIDKLKEKGIWIYGADMCGNDYCFDVNLSGPIALVIGSEGRGISKLTKNKCDVLVKIPMLGNITSLNASV
AGGMLMYEILKQRMKSK

Sequences:

>Translated_257_residues
MKNRVGQQEEIREDIIEGRNAVIEALKSNKTIEKVMVAKGDLEGSIKIIISLAKEKGIVINEVDRKKLDSISQTRAHQGV
IAFTTPYQYCAVEDIIRYAKQKEEDPFIIILDEIEDPHNFGSILRTAEVCGAHGVIIPKRRNVGVTPTVYKTSAGAVEYM
KISKVTNINNVIDKLKEKGIWIYGADMCGNDYCFDVNLSGPIALVIGSEGRGISKLTKNKCDVLVKIPMLGNITSLNASV
AGGMLMYEILKQRMKSK
>Mature_257_residues
MKNRVGQQEEIREDIIEGRNAVIEALKSNKTIEKVMVAKGDLEGSIKIIISLAKEKGIVINEVDRKKLDSISQTRAHQGV
IAFTTPYQYCAVEDIIRYAKQKEEDPFIIILDEIEDPHNFGSILRTAEVCGAHGVIIPKRRNVGVTPTVYKTSAGAVEYM
KISKVTNINNVIDKLKEKGIWIYGADMCGNDYCFDVNLSGPIALVIGSEGRGISKLTKNKCDVLVKIPMLGNITSLNASV
AGGMLMYEILKQRMKSK

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=260, Percent_Identity=26.5384615384615, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1790623, Length=244, Percent_Identity=36.0655737704918, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1790083, Length=168, Percent_Identity=26.7857142857143, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788935, Length=190, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=267, Percent_Identity=28.0898876404494, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324775, Length=169, Percent_Identity=33.1360946745562, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28430; Mature: 28430

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRVGQQEEIREDIIEGRNAVIEALKSNKTIEKVMVAKGDLEGSIKIIISLAKEKGIVI
CCCCCCCHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEHHHCCEEE
NEVDRKKLDSISQTRAHQGVIAFTTPYQYCAVEDIIRYAKQKEEDPFIIILDEIEDPHNF
ECCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
GSILRTAEVCGAHGVIIPKRRNVGVTPTVYKTSAGAVEYMKISKVTNINNVIDKLKEKGI
HHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCCCEEEEHHHHHCCHHHHHHHHHHCCE
WIYGADMCGNDYCFDVNLSGPIALVIGSEGRGISKLTKNKCDVLVKIPMLGNITSLNASV
EEEECCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHCCCCEEEECCCCCCEEECCHHH
AGGMLMYEILKQRMKSK
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNRVGQQEEIREDIIEGRNAVIEALKSNKTIEKVMVAKGDLEGSIKIIISLAKEKGIVI
CCCCCCCHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEHHHCCEEE
NEVDRKKLDSISQTRAHQGVIAFTTPYQYCAVEDIIRYAKQKEEDPFIIILDEIEDPHNF
ECCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
GSILRTAEVCGAHGVIIPKRRNVGVTPTVYKTSAGAVEYMKISKVTNINNVIDKLKEKGI
HHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCCCEEEEHHHHHCCHHHHHHHHHHCCE
WIYGADMCGNDYCFDVNLSGPIALVIGSEGRGISKLTKNKCDVLVKIPMLGNITSLNASV
EEEECCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHCCCCEEEECCCCCCEEECCHHH
AGGMLMYEILKQRMKSK
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 7510287; 3145906 [H]