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The map label for this gene is rnjA [H]

Identifier: 226949914

GI number: 226949914

Start: 2871015

End: 2872724

Strand: Reverse

Name: rnjA [H]

Synonym: CLM_2866

Alternate gene names: 226949914

Gene position: 2872724-2871015 (Counterclockwise)

Preceding gene: 226949915

Following gene: 226949913

Centisome position: 69.13

GC content: 32.63

Gene sequence:

>1710_bases
TTGCGAGTGGACAAAGAAAAAGATAAAGCAAAAGATAGAGTAAAAGATAAGGAGAAGGATAAAATAAAGATTATTCCTTT
AGGCGGCTTAAATGAAATTGGCAAAAATTTAACTGCTATAGAATACAAAAATGATATAGTAGTTATAGATTGTGGCCTTA
AATTTCCAGACGACGATATGTTAGGAATAGATGTAGTTATTCCTGACATAACTTATCTTAAAAAGAATATTAATAAAGTA
AAAGGTATATTTTTAACTCATGGTCATGAAGATCACATAGGAGCATTACCCTATGTTCTGAGAGCAGTAAATGTACCAGT
ATACGGAACTAAGCTTACATTAGGTATAGTAGAAACCAAGTTAAAAGAACATGGACTACTAAGTTCTGTGGAGTTAAAAT
GCATAAAGCCAAGGGAAATAATTAGACTTCAAAATGTTTCTGTGGAATTTATAAGAACTAGCCACAGTATAGCAGACACA
GTAGCTATAGCTATACATACACCTTTAGGGGTTGTTCTTCATACAGGAGACTTTAAAATAGACTACACTCCTATAGATGG
TCATGTTACAGATTTAGCTAGATTTGCTGAAATAGGTAAGCGTGGAGTTATTGCTATGATGGCAGATAGTACAAATGTAG
AAAGACCAGGCTATACCATGTCAGAAAGTACTGTAGGGGAAGCTTTTCTAAATATATTCTCAGGAGTCAAGGGAAGAATA
ATTGTTGCTACTTTTGCATCTCATATCCATAGAATCCAACAAATAATAAGGGCTGCTGAAAAATTTAAAAGAAAAGTAGC
GGTTTCTGGTAGAAGTATGGAAAATATTATGGAAGTAGCTATGAAGCTTGGATATATAAAAGCAGAAAAAAATATATTTA
TAAGCATAGATGCTATAAATAAATATCCAGATGACCAAGTAGTTATAATAACTACAGGAAGTCAAGGAGAGCCTATGTCA
GCTCTTTCTAGGATGGCAGCTTCAGAGCATAAAAAAGTAAATATAAAGCCCGGTGATAAGGTAATTATATCTGCAACACC
TATCCCAGGTAATGAAAAATTAGTATCTAGGGTGATAAATCAGTTATTTAAAAAGGGTGCAGATGTTATATATGAAACAC
TTGCAGATATCCATGTATCTGGTCATGCATGCCAGGAAGAACTTAAACTTATGCATACATTAGTAAGACCTAAATATTTT
ATACCTGTTCATGGAGAATATAGACACTTAAAACAACATGGAGAACTTGCCTGTCAATTAGGATTGCCAGAGAATCATGT
TATGGTTTCAGATATAGGTGATGTTATAGAAGTAAGTAGAGAATGCATTAGAAAAAATGGCTCTGTAGTGTCAGGTCAAG
TCTTTGTAGATGGACTAGGTGTAGGAGATGTAGGGAATATAGTATTAAGAGATAGAAAGCACTTATCTCAAGATGGTATA
TTAACAGTAGTTGTTACTATAGAAAAGGTTTCAGGCTCTGTTATTGCAGGTCCTGATATAATATCAAGAGGTTTTGTTTA
TGTAAGAGAATCAGAGGATTTAATGGAAGAAGCTAAAGATTTAGTAAAAAAAGCTCTAGAAGAATGTGAAAGAAATCATA
TTACAGAATGGGCTGCAATTAAAACAAATATAAAAGATGTTTTAAGAGGATTCTTATATGAAAAAACAAAAAGAAAACCT
ATGATATTACCAATTATAATGGAAATATAG

Upstream 100 bases:

>100_bases
AGAATCATAGTTTAAAGTTCTACGGTATCTGCAAGGAATGTCAAGGAAAATAAATAATAAAACTATGATGAAAACATAAA
AGAAAAAGGAGGGAAGAAGT

Downstream 100 bases:

>100_bases
TACGGTATATTAAAAGAGCTCATAAAAATAATTTTTAATGGAATATTTCAAAGAACTCTTTAATACCTAATAATTTTAAA
AAAAGTAAGCTATGAATTTT

Product: RNA-metabolizing metallo-beta-lactamase family protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MRVDKEKDKAKDRVKDKEKDKIKIIPLGGLNEIGKNLTAIEYKNDIVVIDCGLKFPDDDMLGIDVVIPDITYLKKNINKV
KGIFLTHGHEDHIGALPYVLRAVNVPVYGTKLTLGIVETKLKEHGLLSSVELKCIKPREIIRLQNVSVEFIRTSHSIADT
VAIAIHTPLGVVLHTGDFKIDYTPIDGHVTDLARFAEIGKRGVIAMMADSTNVERPGYTMSESTVGEAFLNIFSGVKGRI
IVATFASHIHRIQQIIRAAEKFKRKVAVSGRSMENIMEVAMKLGYIKAEKNIFISIDAINKYPDDQVVIITTGSQGEPMS
ALSRMAASEHKKVNIKPGDKVIISATPIPGNEKLVSRVINQLFKKGADVIYETLADIHVSGHACQEELKLMHTLVRPKYF
IPVHGEYRHLKQHGELACQLGLPENHVMVSDIGDVIEVSRECIRKNGSVVSGQVFVDGLGVGDVGNIVLRDRKHLSQDGI
LTVVVTIEKVSGSVIAGPDIISRGFVYVRESEDLMEEAKDLVKKALEECERNHITEWAAIKTNIKDVLRGFLYEKTKRKP
MILPIIMEI

Sequences:

>Translated_569_residues
MRVDKEKDKAKDRVKDKEKDKIKIIPLGGLNEIGKNLTAIEYKNDIVVIDCGLKFPDDDMLGIDVVIPDITYLKKNINKV
KGIFLTHGHEDHIGALPYVLRAVNVPVYGTKLTLGIVETKLKEHGLLSSVELKCIKPREIIRLQNVSVEFIRTSHSIADT
VAIAIHTPLGVVLHTGDFKIDYTPIDGHVTDLARFAEIGKRGVIAMMADSTNVERPGYTMSESTVGEAFLNIFSGVKGRI
IVATFASHIHRIQQIIRAAEKFKRKVAVSGRSMENIMEVAMKLGYIKAEKNIFISIDAINKYPDDQVVIITTGSQGEPMS
ALSRMAASEHKKVNIKPGDKVIISATPIPGNEKLVSRVINQLFKKGADVIYETLADIHVSGHACQEELKLMHTLVRPKYF
IPVHGEYRHLKQHGELACQLGLPENHVMVSDIGDVIEVSRECIRKNGSVVSGQVFVDGLGVGDVGNIVLRDRKHLSQDGI
LTVVVTIEKVSGSVIAGPDIISRGFVYVRESEDLMEEAKDLVKKALEECERNHITEWAAIKTNIKDVLRGFLYEKTKRKP
MILPIIMEI
>Mature_569_residues
MRVDKEKDKAKDRVKDKEKDKIKIIPLGGLNEIGKNLTAIEYKNDIVVIDCGLKFPDDDMLGIDVVIPDITYLKKNINKV
KGIFLTHGHEDHIGALPYVLRAVNVPVYGTKLTLGIVETKLKEHGLLSSVELKCIKPREIIRLQNVSVEFIRTSHSIADT
VAIAIHTPLGVVLHTGDFKIDYTPIDGHVTDLARFAEIGKRGVIAMMADSTNVERPGYTMSESTVGEAFLNIFSGVKGRI
IVATFASHIHRIQQIIRAAEKFKRKVAVSGRSMENIMEVAMKLGYIKAEKNIFISIDAINKYPDDQVVIITTGSQGEPMS
ALSRMAASEHKKVNIKPGDKVIISATPIPGNEKLVSRVINQLFKKGADVIYETLADIHVSGHACQEELKLMHTLVRPKYF
IPVHGEYRHLKQHGELACQLGLPENHVMVSDIGDVIEVSRECIRKNGSVVSGQVFVDGLGVGDVGNIVLRDRKHLSQDGI
LTVVVTIEKVSGSVIAGPDIISRGFVYVRESEDLMEEAKDLVKKALEECERNHITEWAAIKTNIKDVLRGFLYEKTKRKP
MILPIIMEI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 63171; Mature: 63171

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDKEKDKAKDRVKDKEKDKIKIIPLGGLNEIGKNLTAIEYKNDIVVIDCGLKFPDDDM
CCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCEEEEECCCEEEEECCCCCCCCCC
LGIDVVIPDITYLKKNINKVKGIFLTHGHEDHIGALPYVLRAVNVPVYGTKLTLGIVETK
EEEEEEECCHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHCCCEECCEEEEHHHHHH
LKEHGLLSSVELKCIKPREIIRLQNVSVEFIRTSHSIADTVAIAIHTPLGVVLHTGDFKI
HHHCCCCCCCEEEEECHHHHHEEECCEEEEEECCCHHHHEEEEEEECCCEEEEECCCEEE
DYTPIDGHVTDLARFAEIGKRGVIAMMADSTNVERPGYTMSESTVGEAFLNIFSGVKGRI
EEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCE
IVATFASHIHRIQQIIRAAEKFKRKVAVSGRSMENIMEVAMKLGYIKAEKNIFISIDAIN
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEEEECCCEEEEEEECC
KYPDDQVVIITTGSQGEPMSALSRMAASEHKKVNIKPGDKVIISATPIPGNEKLVSRVIN
CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHH
QLFKKGADVIYETLADIHVSGHACQEELKLMHTLVRPKYFIPVHGEYRHLKQHGELACQL
HHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCEEEEE
GLPENHVMVSDIGDVIEVSRECIRKNGSVVSGQVFVDGLGVGDVGNIVLRDRKHLSQDGI
CCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCEECCCHHCCCCCE
LTVVVTIEKVSGSVIAGPDIISRGFVYVRESEDLMEEAKDLVKKALEECERNHITEWAAI
EEEEEEEEECCCCEEECCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KTNIKDVLRGFLYEKTKRKPMILPIIMEI
HHHHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MRVDKEKDKAKDRVKDKEKDKIKIIPLGGLNEIGKNLTAIEYKNDIVVIDCGLKFPDDDM
CCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCEEEEECCCEEEEECCCCCCCCCC
LGIDVVIPDITYLKKNINKVKGIFLTHGHEDHIGALPYVLRAVNVPVYGTKLTLGIVETK
EEEEEEECCHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHCCCEECCEEEEHHHHHH
LKEHGLLSSVELKCIKPREIIRLQNVSVEFIRTSHSIADTVAIAIHTPLGVVLHTGDFKI
HHHCCCCCCCEEEEECHHHHHEEECCEEEEEECCCHHHHEEEEEEECCCEEEEECCCEEE
DYTPIDGHVTDLARFAEIGKRGVIAMMADSTNVERPGYTMSESTVGEAFLNIFSGVKGRI
EEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCE
IVATFASHIHRIQQIIRAAEKFKRKVAVSGRSMENIMEVAMKLGYIKAEKNIFISIDAIN
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEEEECCCEEEEEEECC
KYPDDQVVIITTGSQGEPMSALSRMAASEHKKVNIKPGDKVIISATPIPGNEKLVSRVIN
CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHH
QLFKKGADVIYETLADIHVSGHACQEELKLMHTLVRPKYFIPVHGEYRHLKQHGELACQL
HHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCEEEEE
GLPENHVMVSDIGDVIEVSRECIRKNGSVVSGQVFVDGLGVGDVGNIVLRDRKHLSQDGI
CCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCEECCCHHCCCCCE
LTVVVTIEKVSGSVIAGPDIISRGFVYVRESEDLMEEAKDLVKKALEECERNHITEWAAI
EEEEEEEEECCCCEEECCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KTNIKDVLRGFLYEKTKRKPMILPIIMEI
HHHHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]