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The map label for this gene is thiN [H]

Identifier: 226949854

GI number: 226949854

Start: 2810981

End: 2811625

Strand: Reverse

Name: thiN [H]

Synonym: CLM_2801

Alternate gene names: 226949854

Gene position: 2811625-2810981 (Counterclockwise)

Preceding gene: 226949855

Following gene: 226949853

Centisome position: 67.66

GC content: 27.44

Gene sequence:

>645_bases
ATGAAAGCCATAGTAATAGGAGGAGGAGAAGCACCTTCCAAAGAGCTTTTGAAGCAAGAAATGAAGGATTCTTCTTATAT
AATATGTGCGGATAGTGGTGCTAATTGTCTTTATAAATATGATATAATACCTGATTTTATATTAGGAGATATGGATTCTA
TAGATAAAAAAACCTTTTCTTATTTTAAAAAAAAGGGAGTATGTATGGATAAATACCCTAAGGACAAAGATTTTACAGAT
GGTTTAGTAGCTTTAAATAAGGCTATAGAACTTAAAGCAGATACTATAGTTTTATTAGGATGTATAGGTAATAGAATAGA
TCATATATTAGGAAATTTAGGACTTTTAGAAATATGTTTAAAAAATAATATAAAGGCTTATATAAAGGATGAAAATAATG
AAATATTTTTAACAGATAAAGATATTTCATTAAAGCCTAGAAAGTCAAAATATTTTTCATTACAGGCTTATGGGCCTAAT
GTAAAAGGGGTTACACTTTTCAATGCTAAATTTCCATTAGAGAACTATACATTAAAAATGGGGGATACTTTGACAACTTC
AAATGAATTTACGGATAAAGAATTACATGTACACATTAAGAGTGGAACATTAATTGTTATTATAAGCAAAGATAGTGATG
GTTAA

Upstream 100 bases:

>100_bases
ATAGCAGATTGTGGTGCCGATCTTCTAGTAGCAGGCTCAGCAGTATTTAAAAATGGAGAAATAGAAAAAAATATAAAGGT
GCTAAGAGAGGCATTATAAA

Downstream 100 bases:

>100_bases
AGTTTAGCGTGAATTTGTAACAAACTTTACTTGGAACTAAAATTATTTAATAATACCTCTATAAGAGATTTTATTAGTTA
AATGGATTTTATATTGTTAC

Product: thiamine diphosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKAIVIGGGEAPSKELLKQEMKDSSYIICADSGANCLYKYDIIPDFILGDMDSIDKKTFSYFKKKGVCMDKYPKDKDFTD
GLVALNKAIELKADTIVLLGCIGNRIDHILGNLGLLEICLKNNIKAYIKDENNEIFLTDKDISLKPRKSKYFSLQAYGPN
VKGVTLFNAKFPLENYTLKMGDTLTTSNEFTDKELHVHIKSGTLIVIISKDSDG

Sequences:

>Translated_214_residues
MKAIVIGGGEAPSKELLKQEMKDSSYIICADSGANCLYKYDIIPDFILGDMDSIDKKTFSYFKKKGVCMDKYPKDKDFTD
GLVALNKAIELKADTIVLLGCIGNRIDHILGNLGLLEICLKNNIKAYIKDENNEIFLTDKDISLKPRKSKYFSLQAYGPN
VKGVTLFNAKFPLENYTLKMGDTLTTSNEFTDKELHVHIKSGTLIVIISKDSDG
>Mature_214_residues
MKAIVIGGGEAPSKELLKQEMKDSSYIICADSGANCLYKYDIIPDFILGDMDSIDKKTFSYFKKKGVCMDKYPKDKDFTD
GLVALNKAIELKADTIVLLGCIGNRIDHILGNLGLLEICLKNNIKAYIKDENNEIFLTDKDISLKPRKSKYFSLQAYGPN
VKGVTLFNAKFPLENYTLKMGDTLTTSNEFTDKELHVHIKSGTLIVIISKDSDG

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 23850; Mature: 23850

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIVIGGGEAPSKELLKQEMKDSSYIICADSGANCLYKYDIIPDFILGDMDSIDKKTFS
CEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCHHHHCCCCHHHHHHHH
YFKKKGVCMDKYPKDKDFTDGLVALNKAIELKADTIVLLGCIGNRIDHILGNLGLLEICL
HHHHCCCCCCCCCCCCCCCCHHHHHHHHEEECCCEEEEEECHHHHHHHHHCCCCCEEEEH
KNNIKAYIKDENNEIFLTDKDISLKPRKSKYFSLQAYGPNVKGVTLFNAKFPLENYTLKM
HCCCEEEEEECCCEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCEEEEE
GDTLTTSNEFTDKELHVHIKSGTLIVIISKDSDG
CCEEECCCCCCCCEEEEEEECCEEEEEEECCCCC
>Mature Secondary Structure
MKAIVIGGGEAPSKELLKQEMKDSSYIICADSGANCLYKYDIIPDFILGDMDSIDKKTFS
CEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCHHHHCCCCHHHHHHHH
YFKKKGVCMDKYPKDKDFTDGLVALNKAIELKADTIVLLGCIGNRIDHILGNLGLLEICL
HHHHCCCCCCCCCCCCCCCCHHHHHHHHEEECCCEEEEEECHHHHHHHHHCCCCCEEEEH
KNNIKAYIKDENNEIFLTDKDISLKPRKSKYFSLQAYGPNVKGVTLFNAKFPLENYTLKM
HCCCEEEEEECCCEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCEEEEE
GDTLTTSNEFTDKELHVHIKSGTLIVIISKDSDG
CCEEECCCCCCCCEEEEEEECCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]