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The map label for this gene is pnpA [H]

Identifier: 226949765

GI number: 226949765

Start: 2717087

End: 2719195

Strand: Reverse

Name: pnpA [H]

Synonym: CLM_2705

Alternate gene names: 226949765

Gene position: 2719195-2717087 (Counterclockwise)

Preceding gene: 226949766

Following gene: 226949764

Centisome position: 65.44

GC content: 32.15

Gene sequence:

>2109_bases
ATGATTCATACATTAGAAACTACTGTAGCAGGAAGAAAAATGAAAGTAGACTTTGGTAAGACAGGAATGCTTTCAAATGC
TGCCATATTTATGAGTTATGGAGATACGGTAGTTATGATAAATGCCAACGCTTCTAAAGCACCAAGAGAGGGAATAGATT
TTTTCCCATTAAGCGTTGATTATGAAGAAAGACTATATTCAGTAGGAAAAATTCCAGGAGGATTCATTAAAAGAGAAGGA
AAACCTTCTGACAAATCTATATTACATGCCAGATCTATAGATAGACCATTAAGACCTTTATTTCCAAAGGGATATAGAAA
TGATGTTCAAATAGTTAATACAGTTTTATCTGTAGAGCAGGATAATTTACCAGAAATATTAGCTATAAATGGCTCTTCTT
TAGCTTTATGCCTATCTAGCATACCATTTACAACACCAGTAGCTGCCGTTTCTGTAGGCCTAGTAGATGGAGAGTTTATA
ATAAATCCAACTGTAGCCCAAAGAGAAAATACTATACTAGACTTAACTGTATGTGCTACAAAAGAAAGAGTTATGATGGT
AGAAGCAGGTGGACAGGAAATAGATGAGGAAACCATGTATAGTGCTATCATGTTTGGTTTTCAAGAATGTAAAAACATAG
TTGCATTCCAAGAAGAGGCTGTGGCTAAGTTTGGAAAAACTAAAAATGAACCTATATTATATAAAGCAGATGAAGAAGTA
GAGAAAGAAGTTAAAGAATTTGCTTTTGATATGATAAAAGAAGTCATGTATATAATGGATAAAGATGAAAGAAATGCTCA
GTTAGATAAAGTTAAAGAAAAAATATCAGAAGAGTTTTCAGAAAAATATGAAGACAAGAAAGCAGATATAGCAGAAGTTA
TATATAAAACTCAAAAAGAAGTAGTTAGAAATATGCTTTTAAATGAAAATAGAAGACCAGATGGAAGATCTTTTGATGAA
GTAAGACCTATAAGCTGTGAAGTAGGAATACTTCCTAGAACTCATGGTACAGGTTTATTTACAAGAGGATTAACTCAAGT
AATGACTGTAGCTACTTTAGGAGCTTTAGGAGATGTACAAATATTAGATGGTATAGCAGAAGAAGCGTCAAAACGTTATA
TGCATCATTACAATTTCCCATCTTATAGTGTTGGAGAAGTTAGACCTTTAAGAGGACCAGGAAGACGTGAAATTGGTCAC
GGGGCTTTAGCTGAAAGAGCTTTAGAGCCATTAATTCCATCTGAAGAAGAATTCCCATATACTATAAGATTAGTATCAGA
AGTACTAAGTTCAAATGGTTCAACATCACAAGCCAGCGTTTGTGGAAGTACATTAGCTCTATTAGATGCAGGTGTACCTA
TAAAAAGACCAGCTGCAGGTATAGCTATGGGGCTTATAACTAGTGATGATTTAGAAAAAGAAAAAGTAATAACAGATATT
CAAGGTATAGAAGATTTCTTTGGAGATATGGACTTTAAAGTAGCAGGAACAGAAAAAGGAATTACATCTATACAATTTGA
TACAAAAATAGCTGGATTATCAGATAATTGTGTAAGAGATGCATTAGAAGGTGCTAAAAAGGCAAGACTACACATATTAG
GAAAGATAAAAGAATGTATACCAGAACCAAGAAAAGAATTATCTAAATATGCGCCAAGAACAGAAATAATATGTATAGAT
CCAGAAAAAATAAGAGATGTTATAGGTGCTGGTGGAAAGATAATAAATAAAATAATAGCAGATACCAACGTAAAAATAGA
AATAAAAGAAGATGGAAAAATATTTGTAACATCTAATAATGAGCCTGAAGGTGTTAAGAAAGCTATAAGTATAATAGAAG
GATTAACAAAGGAAGTAGTTCAAGGAGAGATTTATCTAGGTAAAGTAACTAAAACTACTAATTTTGGAGCATTTGTAGAA
ATACTACCAGGTAAAGAAGGTCTAGTTCATATATCTAAATTAGATTTTGCTAGAGTAGAAAAAGTGGAAGATGTAGTATC
TGTAGGAGATGAAATATTAGTTAAGGTTACAGACATAGATAATCAAGGAAGAATAAATTTATCACGCAAAGATGCTATTG
CAAAGAAAGAAGAAGAAAAAGATAAATAA

Upstream 100 bases:

>100_bases
ATTAATAGGTTATAAACAAATATAAAAATTAAATGTTTGCTATAAATGTAATAGTGATATAATGATAATATTAGTTCAAT
TATTGAAGGGAGGTTAATGT

Downstream 100 bases:

>100_bases
TAAAAATAGAAGGGCATATCTGCCTTTTTATTTTTTATCAAGTGATTTTTAGGTGCAGGTGGAAGTTACTTATACAGAGT
GCTGATTTGCTGATAATCGC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKAPREGIDFFPLSVDYEERLYSVGKIPGGFIKREG
KPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQDNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFI
INPTVAQRENTILDLTVCATKERVMMVEAGGQEIDEETMYSAIMFGFQECKNIVAFQEEAVAKFGKTKNEPILYKADEEV
EKEVKEFAFDMIKEVMYIMDKDERNAQLDKVKEKISEEFSEKYEDKKADIAEVIYKTQKEVVRNMLLNENRRPDGRSFDE
VRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQILDGIAEEASKRYMHHYNFPSYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSDDLEKEKVITDI
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIAGLSDNCVRDALEGAKKARLHILGKIKECIPEPRKELSKYAPRTEIICID
PEKIRDVIGAGGKIINKIIADTNVKIEIKEDGKIFVTSNNEPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVE
ILPGKEGLVHISKLDFARVEKVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK

Sequences:

>Translated_702_residues
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKAPREGIDFFPLSVDYEERLYSVGKIPGGFIKREG
KPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQDNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFI
INPTVAQRENTILDLTVCATKERVMMVEAGGQEIDEETMYSAIMFGFQECKNIVAFQEEAVAKFGKTKNEPILYKADEEV
EKEVKEFAFDMIKEVMYIMDKDERNAQLDKVKEKISEEFSEKYEDKKADIAEVIYKTQKEVVRNMLLNENRRPDGRSFDE
VRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQILDGIAEEASKRYMHHYNFPSYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSDDLEKEKVITDI
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIAGLSDNCVRDALEGAKKARLHILGKIKECIPEPRKELSKYAPRTEIICID
PEKIRDVIGAGGKIINKIIADTNVKIEIKEDGKIFVTSNNEPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVE
ILPGKEGLVHISKLDFARVEKVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK
>Mature_702_residues
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKAPREGIDFFPLSVDYEERLYSVGKIPGGFIKREG
KPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQDNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFI
INPTVAQRENTILDLTVCATKERVMMVEAGGQEIDEETMYSAIMFGFQECKNIVAFQEEAVAKFGKTKNEPILYKADEEV
EKEVKEFAFDMIKEVMYIMDKDERNAQLDKVKEKISEEFSEKYEDKKADIAEVIYKTQKEVVRNMLLNENRRPDGRSFDE
VRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQILDGIAEEASKRYMHHYNFPSYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSDDLEKEKVITDI
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIAGLSDNCVRDALEGAKKARLHILGKIKECIPEPRKELSKYAPRTEIICID
PEKIRDVIGAGGKIINKIIADTNVKIEIKEDGKIFVTSNNEPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVE
ILPGKEGLVHISKLDFARVEKVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=704, Percent_Identity=37.2159090909091, Blast_Score=437, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=682, Percent_Identity=44.574780058651, Blast_Score=585, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI115534063, Length=725, Percent_Identity=33.7931034482759, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI281362905, Length=704, Percent_Identity=38.3522727272727, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=704, Percent_Identity=38.3522727272727, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=704, Percent_Identity=38.3522727272727, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=649, Percent_Identity=38.6748844375963, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77480; Mature: 77480

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKAPREGIDFFPLSVD
CCEEECHHHCCCEEEECCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCEEEECCC
YEERLYSVGKIPGGFIKREGKPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQ
HHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFIINPTVAQRENTILDLTVCAT
CCCCCEEEECCCHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHCCCCCEEEEEEECC
KERVMMVEAGGQEIDEETMYSAIMFGFQECKNIVAFQEEAVAKFGKTKNEPILYKADEEV
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHH
EKEVKEFAFDMIKEVMYIMDKDERNAQLDKVKEKISEEFSEKYEDKKADIAEVIYKTQKE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVRNMLLNENRRPDGRSFDEVRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQ
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHH
ILDGIAEEASKRYMHHYNFPSYSVGEVRPLRGPGRREIGHGALAERALEPLIPSEEEFPY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCH
TIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSDDLEKEKVITDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHEEEEECCCCCHHHHHHHHH
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIAGLSDNCVRDALEGAKKARLHILGKIKECI
HHHHHHHCCCCEEEECCCCCCEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PEPRKELSKYAPRTEIICIDPEKIRDVIGAGGKIINKIIADTNVKIEIKEDGKIFVTSNN
CCHHHHHHHHCCCCEEEEECHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCEEEEECCC
EPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVEILPGKEGLVHISKLDFARVE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEHHHHHHHH
KVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK
HHHHHHHCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHCCC
>Mature Secondary Structure
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKAPREGIDFFPLSVD
CCEEECHHHCCCEEEECCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCEEEECCC
YEERLYSVGKIPGGFIKREGKPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQ
HHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFIINPTVAQRENTILDLTVCAT
CCCCCEEEECCCHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHCCCCCEEEEEEECC
KERVMMVEAGGQEIDEETMYSAIMFGFQECKNIVAFQEEAVAKFGKTKNEPILYKADEEV
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHH
EKEVKEFAFDMIKEVMYIMDKDERNAQLDKVKEKISEEFSEKYEDKKADIAEVIYKTQKE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVRNMLLNENRRPDGRSFDEVRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQ
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHH
ILDGIAEEASKRYMHHYNFPSYSVGEVRPLRGPGRREIGHGALAERALEPLIPSEEEFPY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCH
TIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSDDLEKEKVITDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHEEEEECCCCCHHHHHHHHH
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIAGLSDNCVRDALEGAKKARLHILGKIKECI
HHHHHHHCCCCEEEECCCCCCEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PEPRKELSKYAPRTEIICIDPEKIRDVIGAGGKIINKIIADTNVKIEIKEDGKIFVTSNN
CCHHHHHHHHCCCCEEEEECHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCEEEEECCC
EPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVEILPGKEGLVHISKLDFARVE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEHHHHHHHH
KVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK
HHHHHHHCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA