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The map label for this gene is polA [C]

Identifier: 226949733

GI number: 226949733

Start: 2687749

End: 2689716

Strand: Reverse

Name: polA [C]

Synonym: CLM_2673

Alternate gene names: 226949733

Gene position: 2689716-2687749 (Counterclockwise)

Preceding gene: 226949734

Following gene: 226949732

Centisome position: 64.73

GC content: 33.69

Gene sequence:

>1968_bases
ATGACTACACTTGCTATAGATGTTGAAACATATAGCAGTATAGATATTAAAACTTCGGGGGCCTACAAATATTGTGAGGC
TCCTGATTTTGAAATATTATTATTTGCTTATGCTTTCAATGATGAACCAGTACAAATTGTAGATTTCACTCAACATGAAG
CATTACCACAAAGAGTTATAGATGCACTTTATGATCCTGATGTAATTAAAACTGCATTTAATGCAAATTTTGAAAGAAGT
GCTATAAAATTTGGTGTGTCTAGCCTACCGTGCCCACCTGGGCAATGGGAATGCACAATGATAAAAGCTTTAACACTAGG
GCTACCAAGCTCCCTTGATATGGTTGGTAAGGCTTTAAAGCTTGAAGAAGATAAGCAAAAGATGAAAGAGGGTAAAGCAT
TAATACAATATTTTTGTAAGCCTTGTAAACCTACTAAAACAAATGATAAGAGAACCAGGAATCTTCCAAAACATGATATG
GAGAAATGGGAAACTTTTAAAGAATACTGTAAGCGAGATGTTGAAGTAGAAAGAGAAATAAGAAATTTATTAAATAGATA
TGAAACTAAAGCAGAAGAACAAAAATTATGGCAGCTGGACCAACACATAAATGATAGAGGTATTGATACTGATTTAATAC
TTATAAAGCAGGCTATTGAATGTGATACAAACTATACTGAAAGATTAACAAAAGCTGCTATTAAACTTACAGGACTTGAA
AATCCAAATAGTCCTACACAAATAAAAAAATGGATTGGTGAAAGGCTTGGAAGAGAGGTAAAGTCATTAACTAAAACAAG
TATTCCAGGACTTATAGAAGAAGCCAAGGATTTAAATAAAAGCGAAGTTATAAAAATGTTAGAACTAAGGCAACTAATGG
CCAAGACTTCTATTAAGAAATATGACACAATGCAAAATGCAAGATGTAAAGATGGTAGAGTAAGAGGTTTATTACAATTC
TATGGAGCTAACAGGACAGGCAGATGGGCAGGAAGATTAGTTCAAGTACAAAACTTACCTCAAAATCATTTACCTGATCT
AGACAATGCTAGGAATTTTGTTAGAGAGGGAAAATTTGAAGAAGTAGAATTTTTATTTGATAGTGTTCCGGATACATTAA
GTCAATTAATAAGAACAGCCTTTATACCTAGAGAAGGTAATAGATTTATAGTATCAGACTTTAGTGCTATAGAAGCTAGG
GTTATAGCGTGGTTTGCCGGGGAGCAATGGAGATTAGATGTTTTTAGTACTCATGGAAAGATATATGAAGCTTCAGCTAG
TCAAATGTTTAAAGTTCCAATAGAAAGCATAAAAAAGGGTTCAGAGTTAAGGCAAAAGGGAAAAATAGCAGAATTGGCAC
TTGGATATGGTGGAAGTGTTGGGGCTCTTAGTTCAATGGATAGAAAAAAGAGTATTCCAGAGGAAGAACTTCCCGGACTA
GTTAAAAGTTGGCGAAATGCTAATCCTAATATAACTAAATTTTGGTGGGATGTAGACAAGGCTGCTAAAAAGGCTATAAG
AGAAAGAACTACAGTAAATTTGCAATATGGACTTAAATTTATATATGATCCAGGAGTTTTATTTATACAACTACCTAGTG
GAAGAAAGCTAAGTTATATAAGGCCAAAGATAGAACCGCATGAAACTTTTAGTGGGGATAAAATTACCTATGAAGGTATG
GAACAAACATCTAAGCAATGGAAAAGAATAGATACTTATGGTCCTAAACTAGTGGAGAATATAGTACAAGCTACATCTAG
AGATTGTTTAAGAGAAGCTATGTTTAGAGTAAATAAAGAAGGATATGACATAGTAATGCATGTGCATGATGAAGTTGTAT
TAGATATACCAAAAGATAGGGCTACGGTTAAAGAAATAAATGAATTAATGGGCCAGCCAATAAAGTGGGCTCCAGGATTA
CCTTTGAAAGCTGATGGTTATGAATGCAATTACTATATGAAAGATTGA

Upstream 100 bases:

>100_bases
AAAAGTTAGAAGATGGTGAACCTCTTGGAGGATTCACAAGAGCAGAGGACGACTTTGATTCTGTAGAAACTGCAGAAGAT
GACTTCCTAGGTTAAGGATT

Downstream 100 bases:

>100_bases
TGTAATAAAATTAAAGCCAAGTAAGAGGAGTGATAGATTTGAAGGTCGTTTTAGTAAAAGAAAATAATAAAATTAGAGTT
TTAGAAGGTAAAGGAACAAT

Product: DNA polymerase

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: DNA Polymerase I; DNA Polymerase; DNA Polymerase Bacteriophage Origin; Phage-Related DNA Polymerase; Phage Related DNA Polymerase; Family A DNA-Dependent DNA Polymerase; DNA Polymerase Type I; Prophage DNA Polymerase; DNA-Dependent DNA Polymerase Family A; Prophage; DNA-Directed DNA Polymerase I; DNA Polymerase I Domain Protein; Truncated Phage-Related DNA Polymerase; DNA-Directed Dna Polymerase I; APSE-2 Prophage

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MTTLAIDVETYSSIDIKTSGAYKYCEAPDFEILLFAYAFNDEPVQIVDFTQHEALPQRVIDALYDPDVIKTAFNANFERS
AIKFGVSSLPCPPGQWECTMIKALTLGLPSSLDMVGKALKLEEDKQKMKEGKALIQYFCKPCKPTKTNDKRTRNLPKHDM
EKWETFKEYCKRDVEVEREIRNLLNRYETKAEEQKLWQLDQHINDRGIDTDLILIKQAIECDTNYTERLTKAAIKLTGLE
NPNSPTQIKKWIGERLGREVKSLTKTSIPGLIEEAKDLNKSEVIKMLELRQLMAKTSIKKYDTMQNARCKDGRVRGLLQF
YGANRTGRWAGRLVQVQNLPQNHLPDLDNARNFVREGKFEEVEFLFDSVPDTLSQLIRTAFIPREGNRFIVSDFSAIEAR
VIAWFAGEQWRLDVFSTHGKIYEASASQMFKVPIESIKKGSELRQKGKIAELALGYGGSVGALSSMDRKKSIPEEELPGL
VKSWRNANPNITKFWWDVDKAAKKAIRERTTVNLQYGLKFIYDPGVLFIQLPSGRKLSYIRPKIEPHETFSGDKITYEGM
EQTSKQWKRIDTYGPKLVENIVQATSRDCLREAMFRVNKEGYDIVMHVHDEVVLDIPKDRATVKEINELMGQPIKWAPGL
PLKADGYECNYYMKD

Sequences:

>Translated_655_residues
MTTLAIDVETYSSIDIKTSGAYKYCEAPDFEILLFAYAFNDEPVQIVDFTQHEALPQRVIDALYDPDVIKTAFNANFERS
AIKFGVSSLPCPPGQWECTMIKALTLGLPSSLDMVGKALKLEEDKQKMKEGKALIQYFCKPCKPTKTNDKRTRNLPKHDM
EKWETFKEYCKRDVEVEREIRNLLNRYETKAEEQKLWQLDQHINDRGIDTDLILIKQAIECDTNYTERLTKAAIKLTGLE
NPNSPTQIKKWIGERLGREVKSLTKTSIPGLIEEAKDLNKSEVIKMLELRQLMAKTSIKKYDTMQNARCKDGRVRGLLQF
YGANRTGRWAGRLVQVQNLPQNHLPDLDNARNFVREGKFEEVEFLFDSVPDTLSQLIRTAFIPREGNRFIVSDFSAIEAR
VIAWFAGEQWRLDVFSTHGKIYEASASQMFKVPIESIKKGSELRQKGKIAELALGYGGSVGALSSMDRKKSIPEEELPGL
VKSWRNANPNITKFWWDVDKAAKKAIRERTTVNLQYGLKFIYDPGVLFIQLPSGRKLSYIRPKIEPHETFSGDKITYEGM
EQTSKQWKRIDTYGPKLVENIVQATSRDCLREAMFRVNKEGYDIVMHVHDEVVLDIPKDRATVKEINELMGQPIKWAPGL
PLKADGYECNYYMKD
>Mature_654_residues
TTLAIDVETYSSIDIKTSGAYKYCEAPDFEILLFAYAFNDEPVQIVDFTQHEALPQRVIDALYDPDVIKTAFNANFERSA
IKFGVSSLPCPPGQWECTMIKALTLGLPSSLDMVGKALKLEEDKQKMKEGKALIQYFCKPCKPTKTNDKRTRNLPKHDME
KWETFKEYCKRDVEVEREIRNLLNRYETKAEEQKLWQLDQHINDRGIDTDLILIKQAIECDTNYTERLTKAAIKLTGLEN
PNSPTQIKKWIGERLGREVKSLTKTSIPGLIEEAKDLNKSEVIKMLELRQLMAKTSIKKYDTMQNARCKDGRVRGLLQFY
GANRTGRWAGRLVQVQNLPQNHLPDLDNARNFVREGKFEEVEFLFDSVPDTLSQLIRTAFIPREGNRFIVSDFSAIEARV
IAWFAGEQWRLDVFSTHGKIYEASASQMFKVPIESIKKGSELRQKGKIAELALGYGGSVGALSSMDRKKSIPEEELPGLV
KSWRNANPNITKFWWDVDKAAKKAIRERTTVNLQYGLKFIYDPGVLFIQLPSGRKLSYIRPKIEPHETFSGDKITYEGME
QTSKQWKRIDTYGPKLVENIVQATSRDCLREAMFRVNKEGYDIVMHVHDEVVLDIPKDRATVKEINELMGQPIKWAPGLP
LKADGYECNYYMKD

Specific function: In Addition To Polymerase Activity, This DNA Polymerase Exhibits 3' To 5' And 5' To 3' Exonuclease Activity. It Is Able To Utilize Nicked Circular Duplex DNA As A Template And Can Unwind The Parental DNA Strand From Its Template. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 75176; Mature: 75045

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLAIDVETYSSIDIKTSGAYKYCEAPDFEILLFAYAFNDEPVQIVDFTQHEALPQRVI
CCEEEEEEECCCCEEEEECCCEEECCCCCCEEEEEEEEECCCCEEEEECHHHHHHHHHHH
DALYDPDVIKTAFNANFERSAIKFGVSSLPCPPGQWECTMIKALTLGLPSSLDMVGKALK
HHHCCCHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCCCCCHHHHHHHHH
LEEDKQKMKEGKALIQYFCKPCKPTKTNDKRTRNLPKHDMEKWETFKEYCKRDVEVEREI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHH
RNLLNRYETKAEEQKLWQLDQHINDRGIDTDLILIKQAIECDTNYTERLTKAAIKLTGLE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHEECCC
NPNSPTQIKKWIGERLGREVKSLTKTSIPGLIEEAKDLNKSEVIKMLELRQLMAKTSIKK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YDTMQNARCKDGRVRGLLQFYGANRTGRWAGRLVQVQNLPQNHLPDLDNARNFVREGKFE
HHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHCCCHH
EVEFLFDSVPDTLSQLIRTAFIPREGNRFIVSDFSAIEARVIAWFAGEQWRLDVFSTHGK
HHHHHHHHCHHHHHHHHHHHCCCCCCCEEEEECHHHHHHEEEEEECCCCEEEEEEECCCC
IYEASASQMFKVPIESIKKGSELRQKGKIAELALGYGGSVGALSSMDRKKSIPEEELPGL
EEECCCHHHHHCCHHHHHCCHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCHH
VKSWRNANPNITKFWWDVDKAAKKAIRERTTVNLQYGLKFIYDPGVLFIQLPSGRKLSYI
HHHHCCCCCCCEEEEECHHHHHHHHHHHHCEEEHEECEEEEECCCEEEEECCCCCEEEEE
RPKIEPHETFSGDKITYEGMEQTSKQWKRIDTYGPKLVENIVQATSRDCLREAMFRVNKE
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GYDIVMHVHDEVVLDIPKDRATVKEINELMGQPIKWAPGLPLKADGYECNYYMKD
CCEEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TTLAIDVETYSSIDIKTSGAYKYCEAPDFEILLFAYAFNDEPVQIVDFTQHEALPQRVI
CEEEEEEECCCCEEEEECCCEEECCCCCCEEEEEEEEECCCCEEEEECHHHHHHHHHHH
DALYDPDVIKTAFNANFERSAIKFGVSSLPCPPGQWECTMIKALTLGLPSSLDMVGKALK
HHHCCCHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCCCCCHHHHHHHHH
LEEDKQKMKEGKALIQYFCKPCKPTKTNDKRTRNLPKHDMEKWETFKEYCKRDVEVEREI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHH
RNLLNRYETKAEEQKLWQLDQHINDRGIDTDLILIKQAIECDTNYTERLTKAAIKLTGLE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHEECCC
NPNSPTQIKKWIGERLGREVKSLTKTSIPGLIEEAKDLNKSEVIKMLELRQLMAKTSIKK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YDTMQNARCKDGRVRGLLQFYGANRTGRWAGRLVQVQNLPQNHLPDLDNARNFVREGKFE
HHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHCCCHH
EVEFLFDSVPDTLSQLIRTAFIPREGNRFIVSDFSAIEARVIAWFAGEQWRLDVFSTHGK
HHHHHHHHCHHHHHHHHHHHCCCCCCCEEEEECHHHHHHEEEEEECCCCEEEEEEECCCC
IYEASASQMFKVPIESIKKGSELRQKGKIAELALGYGGSVGALSSMDRKKSIPEEELPGL
EEECCCHHHHHCCHHHHHCCHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCHH
VKSWRNANPNITKFWWDVDKAAKKAIRERTTVNLQYGLKFIYDPGVLFIQLPSGRKLSYI
HHHHCCCCCCCEEEEECHHHHHHHHHHHHCEEEHEECEEEEECCCEEEEECCCCCEEEEE
RPKIEPHETFSGDKITYEGMEQTSKQWKRIDTYGPKLVENIVQATSRDCLREAMFRVNKE
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GYDIVMHVHDEVVLDIPKDRATVKEINELMGQPIKWAPGLPLKADGYECNYYMKD
CCEEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA