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The map label for this gene is yfiJ [H]

Identifier: 226948657

GI number: 226948657

Start: 1612375

End: 1613532

Strand: Direct

Name: yfiJ [H]

Synonym: CLM_1552

Alternate gene names: 226948657

Gene position: 1612375-1613532 (Clockwise)

Preceding gene: 226948656

Following gene: 226948658

Centisome position: 38.8

GC content: 26.42

Gene sequence:

>1158_bases
ATGGAGAGAAAAAAATTACTAATTATATTTAGATACATAGCTATATTATTTCTTATATTTGGTATAGCTTCTTTTGAAAA
AGAAAATATTACAACTAATACGGTTATTTTAATATTATTATTTATTATAAATAATCAAATCAGATTTTTTGTTTTAAATA
AAAATAAGAATTTAGTTTTGATTTCTATAATGTTAGAGTGTATTTTAGCTTACATTGGATATATTAATTATTCTGGAATA
TTGTTTTTTTATTTTTTTACAGATATTTTGGATTCAGCTCTTTTTCTTAAAGATGGGATATCTTACTTAACCTGTTCTAT
AATATTAATTATAGTCATATTTCTCGGATGGAATTTAAATTTAAGCGAAATTTTATCTATAGTAACTTCTTTTATTATGT
TGTTTATATTAGCTACTTATATAAAAGAAGAAAATAGCGGTAAATTAAGGGCGCAAAAATTATATGATAAACTTAGAATT
TCGGAAGAAAAATTAAAAAAAGCTAATAAGGACTTAGAGAGTTATGCCAATTCTATAGAAGAGCTTACAGTTCTCCGTGA
AAGAAATAGAATATCCAGAGAAATACATGATAGTGTAGGACATAGTTTATCTACAATGATAATACAATTAGGAGCTATAG
AAAAGATTGCCGAAAAGGATGAGAAACTCACATCAGAAATGGCGAGGAATTTAGGTGAGTTTGCTAAAAATAGTTTAGGA
GAAGTAAGGTGTGCTGTAAGAGCATTAAAACCTAGAGAATTTGAAAAATATGAAGGTATATTAGCAATAGAAGAACTTAC
AAAGAATTTTGAAAAACTTACAGGGGTAAAAGTAAATCTGGGATTTTCAAAAGAAAAATGGACTCTTAATTCAGATCAAT
GTTTTGTTTTATATAGGGTAGTTCAGGAATTCTTATCCAACTCTTTAAGACATGGAAGCGCCACAAGAGTTGATATATTT
ATGGGATTTAATGAAGAAAATTTAATAATAACTCTTAGAGATAATGGGCAAGGGGTGGATAAAATAGAAAAAGGTGTAGG
CCTTAAAAGTATATGGGAAAGAGTTAATGAATTAGGTGGAACTGTAGCATATAATAGTAAAAAAGAAGAAGGATTTTTAT
TAAAAGTAGTATTATATCCACAAATTAATGAAGCCTAA

Upstream 100 bases:

>100_bases
AGTCTATAATATAAATAATAGGGATAGTTTATTACAAATTAGTTTTTAAGGACTAAGTTAATAAACGGTCCTTATTTAAA
AATAATAAAGGATTAAAAAT

Downstream 100 bases:

>100_bases
TAATTTTCATTATAAACAGAGTTCTTGACTTCAGAGGGAGTTTTTACTCCCTCTGAAGCTTATTAACAGAATTCTTAGGA
GTTTTACTCCTTAGAAGTCG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MERKKLLIIFRYIAILFLIFGIASFEKENITTNTVILILLFIINNQIRFFVLNKNKNLVLISIMLECILAYIGYINYSGI
LFFYFFTDILDSALFLKDGISYLTCSIILIIVIFLGWNLNLSEILSIVTSFIMLFILATYIKEENSGKLRAQKLYDKLRI
SEEKLKKANKDLESYANSIEELTVLRERNRISREIHDSVGHSLSTMIIQLGAIEKIAEKDEKLTSEMARNLGEFAKNSLG
EVRCAVRALKPREFEKYEGILAIEELTKNFEKLTGVKVNLGFSKEKWTLNSDQCFVLYRVVQEFLSNSLRHGSATRVDIF
MGFNEENLIITLRDNGQGVDKIEKGVGLKSIWERVNELGGTVAYNSKKEEGFLLKVVLYPQINEA

Sequences:

>Translated_385_residues
MERKKLLIIFRYIAILFLIFGIASFEKENITTNTVILILLFIINNQIRFFVLNKNKNLVLISIMLECILAYIGYINYSGI
LFFYFFTDILDSALFLKDGISYLTCSIILIIVIFLGWNLNLSEILSIVTSFIMLFILATYIKEENSGKLRAQKLYDKLRI
SEEKLKKANKDLESYANSIEELTVLRERNRISREIHDSVGHSLSTMIIQLGAIEKIAEKDEKLTSEMARNLGEFAKNSLG
EVRCAVRALKPREFEKYEGILAIEELTKNFEKLTGVKVNLGFSKEKWTLNSDQCFVLYRVVQEFLSNSLRHGSATRVDIF
MGFNEENLIITLRDNGQGVDKIEKGVGLKSIWERVNELGGTVAYNSKKEEGFLLKVVLYPQINEA
>Mature_385_residues
MERKKLLIIFRYIAILFLIFGIASFEKENITTNTVILILLFIINNQIRFFVLNKNKNLVLISIMLECILAYIGYINYSGI
LFFYFFTDILDSALFLKDGISYLTCSIILIIVIFLGWNLNLSEILSIVTSFIMLFILATYIKEENSGKLRAQKLYDKLRI
SEEKLKKANKDLESYANSIEELTVLRERNRISREIHDSVGHSLSTMIIQLGAIEKIAEKDEKLTSEMARNLGEFAKNSLG
EVRCAVRALKPREFEKYEGILAIEELTKNFEKLTGVKVNLGFSKEKWTLNSDQCFVLYRVVQEFLSNSLRHGSATRVDIF
MGFNEENLIITLRDNGQGVDKIEKGVGLKSIWERVNELGGTVAYNSKKEEGFLLKVVLYPQINEA

Specific function: Member of the two-component regulatory system yfiJ/yfiK. Probably activates yfiK by phosphorylation [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=223, Percent_Identity=25.1121076233184, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788812, Length=201, Percent_Identity=21.8905472636816, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082311, Length=183, Percent_Identity=27.3224043715847, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011712 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44186; Mature: 44186

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKKLLIIFRYIAILFLIFGIASFEKENITTNTVILILLFIINNQIRFFVLNKNKNLVL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEH
ISIMLECILAYIGYINYSGILFFYFFTDILDSALFLKDGISYLTCSIILIIVIFLGWNLN
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSEILSIVTSFIMLFILATYIKEENSGKLRAQKLYDKLRISEEKLKKANKDLESYANSIE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ELTVLRERNRISREIHDSVGHSLSTMIIQLGAIEKIAEKDEKLTSEMARNLGEFAKNSLG
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVRCAVRALKPREFEKYEGILAIEELTKNFEKLTGVKVNLGFSKEKWTLNSDQCFVLYRV
HHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHH
VQEFLSNSLRHGSATRVDIFMGFNEENLIITLRDNGQGVDKIEKGVGLKSIWERVNELGG
HHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCC
TVAYNSKKEEGFLLKVVLYPQINEA
EEEECCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MERKKLLIIFRYIAILFLIFGIASFEKENITTNTVILILLFIINNQIRFFVLNKNKNLVL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEH
ISIMLECILAYIGYINYSGILFFYFFTDILDSALFLKDGISYLTCSIILIIVIFLGWNLN
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSEILSIVTSFIMLFILATYIKEENSGKLRAQKLYDKLRISEEKLKKANKDLESYANSIE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ELTVLRERNRISREIHDSVGHSLSTMIIQLGAIEKIAEKDEKLTSEMARNLGEFAKNSLG
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVRCAVRALKPREFEKYEGILAIEELTKNFEKLTGVKVNLGFSKEKWTLNSDQCFVLYRV
HHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHH
VQEFLSNSLRHGSATRVDIFMGFNEENLIITLRDNGQGVDKIEKGVGLKSIWERVNELGG
HHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCC
TVAYNSKKEEGFLLKVVLYPQINEA
EEEECCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]