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The map label for this gene is topB [H]

Identifier: 226948556

GI number: 226948556

Start: 1499310

End: 1501505

Strand: Direct

Name: topB [H]

Synonym: CLM_1448

Alternate gene names: 226948556

Gene position: 1499310-1501505 (Clockwise)

Preceding gene: 226948555

Following gene: 226948557

Centisome position: 36.08

GC content: 30.01

Gene sequence:

>2196_bases
ATGGGAAAAGTATTAGTTTTAGCAGAAAAACCTAGTGTAGGAAGAGATTTAGCTAAGGTTTTAAAATGCAATAAGAAGGG
TAATGGTTATTTAGAAGGAAATAAATATATTGTAACTTGGGCCTTAGGACATTTAATAACTTTATCAGATCCTGAAAGTT
ACGATGAAAAATATAAAAAATGGAGTATGGATACCCTTCCAATGCTACCTAAGAGAATGAAAACTACAGTTATAAAGAAG
ACTTCAAAACATTTTAGTGAAGTTAGAAAAGTTATGACAAGAAAAGATGTAGAAGAACTTGTTATAGCTACAGATGCGGG
AAGAGAAGGTGAGCTTGTAGCTAGATGGATAATTGATAAAGTAGGTTTTAAAAAGCCTATAAAACGTCTTTGGATTTCTT
CGCAAACAGATAAAGCTATTTTAGATGGGTTTAGAAATTTAAAACCGGGAAAAAATTATGAAAATTTATATCATTCAGCA
GTGTGTAGATCAGAAGCAGATTGGATTGTAGGACTTAATGTTACAAGAGCTTTGACTTGTAGATATAATGCTCAACTTTC
AGCAGGAAGAGTTCAAACACCTACTCTAGCTATGATAGTTCAAAGGGAAGAAGAAATAAAGAATTTCAAACCTAGAGATT
ATTATAGTATAGAAGGTAAGACTAAAGGGTTTATAATGCATTGGGAAAATGCTAAAGGTGGATTTAATACCTTTAATGAA
GATTTAGCTAAAAAAGTAGTTTCTAAGGTTACAGGTAAAGAGGGAAGAATAATAGAGGTAACGGAAAGTAGTAAAAAGAA
ATATGCTCCGGCTCTTTATGATTTAACAGAACTTCAAAGAGACGCTAATAGAATATTTGGCTATTCAGCAAAACAAACCC
TATCTATAATGCAAAGATTATATGAAAATCATAAAATTTTAACTTATCCAAGAACGGATTCTAGATATATTTCAAAGGAT
ATTGTTGATACTATAAAAGATAGATTAAAGGCTATATCTATAGGAAATTATAGCAAGTTTACAAATGAAATATTAAAAGG
AAACATTAAGGCACATAAAGGTTTTGTAGATGATAGTAAGGTTTCAGACCATCATGCTATAATTCCTACAGAAGAAAAAC
CTAAATTAGGTAACCTAAGTGGTGAGGAAAGAAATATTTATGATTTAGTTATTAAAAGATTTTTATCAGTTATGTTACCA
CCCTTTGAATATATTCAAACTACCATTAAATCAGAAGTTATAGGTGAAAAATTTGTAGCTAGAGGAAAGGTTATCAAATC
CAAGGGATGGAAAGCAGTTTATGATAGAGAAGAATTCGATGATAAAAATAATAATTCAGATAGTGATGATATAAAAGATC
AAACTCTTCCTAAGGTTAATAAGGGAGATGGTATAAAATTTTCTAACTTTAAGATAAACAAAGGTCAAACAAAGCCGCCA
GCTAGATTCAATGAGGGAACTTTATTATCTGCTATGGAGAATCCTCAAAGATATATTAATTTAGATAAGGAGAGTTCTAA
AACCTTAGGAGAAACTGGTGGACTTGGAACTGTTGCAACTAGAGCAGATATAATTGAAAAATTATATAATACTTTTTATA
TAGAAAAAAGAGGAAAAGAAATAGTTCCAACTTCAAAGGGAAAGCAAGTTATAGATTTAGTACCTAAGGATTTGAAATCC
CCACTTTTAACAGCGAAATGGGAAAGAGAATTAGATTCCATTAGTAAGGGTAATTTAAGAGGACAGATATTTATAAATAA
AATGAGAGACTATTCTACAAAACTTGTAGAAGATGTAAAAAATAGCAAGGATAAATATGTTCATGATAATTTAACTGGTA
AAAAATGTCCACAATGTGGCAAATATATGTTAGAAGTTAAGGGTAAAAATGGAGTTATAAATGTATGCCAGGATAGAGAA
TGTGGCTATAGAGAAAATGTAAGCAGATTTACCAATGTAAGATGTCCTGAGTGTCATGTAAAATTAGAACTTAGAGGAGA
AGGGCAGGGTCAAATCTATGTATGTACAAGATGTAGTTTTAGAGAAAAACTTTCTTCCTTTAATAAAAAGTATAGAAACA
ATCAAGAAAAATTAAATAAAAGAGATGTAGCTAAATATATGAAAAAAATGCAAAAAGAAAATGACGAACCAATAAATTCA
GCTTTAGCAGAGGCTTTATCTAAATTGAAATTATAA

Upstream 100 bases:

>100_bases
TTAAATATATAATTATATATATGTATGTAAGGGTAGTAAGTATATCTATCCTTATTTTTTATTAAAAAAAGAATTATTTA
TGGAAGGACGATAATTATTT

Downstream 100 bases:

>100_bases
AGAATATGCTATAATAGATGTAATTTTAAATAAGAGAATATATTTTTCAAATAAAAATTCCTTGACCTTATAGTCCAATA
TACAATATAATTAAAACGAA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MGKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKKWSMDTLPMLPKRMKTTVIKK
TSKHFSEVRKVMTRKDVEELVIATDAGREGELVARWIIDKVGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSA
VCRSEADWIVGLNVTRALTCRYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFIMHWENAKGGFNTFNE
DLAKKVVSKVTGKEGRIIEVTESSKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRLYENHKILTYPRTDSRYISKD
IVDTIKDRLKAISIGNYSKFTNEILKGNIKAHKGFVDDSKVSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLP
PFEYIQTTIKSEVIGEKFVARGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFKINKGQTKPP
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKEIVPTSKGKQVIDLVPKDLKS
PLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVKNSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRE
CGYRENVSRFTNVRCPECHVKLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVAKYMKKMQKENDEPINS
ALAEALSKLKL

Sequences:

>Translated_731_residues
MGKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKKWSMDTLPMLPKRMKTTVIKK
TSKHFSEVRKVMTRKDVEELVIATDAGREGELVARWIIDKVGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSA
VCRSEADWIVGLNVTRALTCRYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFIMHWENAKGGFNTFNE
DLAKKVVSKVTGKEGRIIEVTESSKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRLYENHKILTYPRTDSRYISKD
IVDTIKDRLKAISIGNYSKFTNEILKGNIKAHKGFVDDSKVSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLP
PFEYIQTTIKSEVIGEKFVARGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFKINKGQTKPP
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKEIVPTSKGKQVIDLVPKDLKS
PLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVKNSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRE
CGYRENVSRFTNVRCPECHVKLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVAKYMKKMQKENDEPINS
ALAEALSKLKL
>Mature_730_residues
GKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKKWSMDTLPMLPKRMKTTVIKKT
SKHFSEVRKVMTRKDVEELVIATDAGREGELVARWIIDKVGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSAV
CRSEADWIVGLNVTRALTCRYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFIMHWENAKGGFNTFNED
LAKKVVSKVTGKEGRIIEVTESSKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRLYENHKILTYPRTDSRYISKDI
VDTIKDRLKAISIGNYSKFTNEILKGNIKAHKGFVDDSKVSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLPP
FEYIQTTIKSEVIGEKFVARGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFKINKGQTKPPA
RFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKEIVPTSKGKQVIDLVPKDLKSP
LLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVKNSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDREC
GYRENVSRFTNVRCPECHVKLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVAKYMKKMQKENDEPINSA
LAEALSKLKL

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=695, Percent_Identity=24.6043165467626, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI4507635, Length=752, Percent_Identity=22.8723404255319, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI1788061, Length=621, Percent_Identity=31.5619967793881, Blast_Score=338, Evalue=8e-94,
Organism=Escherichia coli, GI1787529, Length=705, Percent_Identity=25.2482269503546, Blast_Score=168, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI32563869, Length=694, Percent_Identity=24.6397694524496, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17555378, Length=598, Percent_Identity=25.0836120401338, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=702, Percent_Identity=22.2222222222222, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24640096, Length=713, Percent_Identity=24.5441795231417, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24585251, Length=574, Percent_Identity=24.2160278745645, Blast_Score=114, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83593; Mature: 83462

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKK
CCCEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCEEEEEECCCEEECCCCHHHHHHHHH
WSMDTLPMLPKRMKTTVIKKTSKHFSEVRKVMTRKDVEELVIATDAGREGELVARWIIDK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCHHHHHHHHH
VGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSAVCRSEADWIVGLNVTRALTC
HCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEE
RYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFIMHWENAKGGFNTFNE
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHH
DLAKKVVSKVTGKEGRIIEVTESSKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRL
HHHHHHHHHHCCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YENHKILTYPRTDSRYISKDIVDTIKDRLKAISIGNYSKFTNEILKGNIKAHKGFVDDSK
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCHHHCCCCCCCC
VSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLPPFEYIQTTIKSEVIGEKFVA
CCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFKINKGQTKPP
CCCEECCCCCCEECCCHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCC
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKE
CCCCCCCEEHHHHCCHHEEECCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
IVPTSKGKQVIDLVPKDLKSPLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVK
CCCCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
NSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRECGYRENVSRFTNVRCPECHV
CCCCCEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEE
KLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVAKYMKKMQKENDEPINS
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
ALAEALSKLKL
HHHHHHHHHCC
>Mature Secondary Structure 
GKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKK
CCEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCEEEEEECCCEEECCCCHHHHHHHHH
WSMDTLPMLPKRMKTTVIKKTSKHFSEVRKVMTRKDVEELVIATDAGREGELVARWIIDK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCHHHHHHHHH
VGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSAVCRSEADWIVGLNVTRALTC
HCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEE
RYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFIMHWENAKGGFNTFNE
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHH
DLAKKVVSKVTGKEGRIIEVTESSKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRL
HHHHHHHHHHCCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YENHKILTYPRTDSRYISKDIVDTIKDRLKAISIGNYSKFTNEILKGNIKAHKGFVDDSK
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCHHHCCCCCCCC
VSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLPPFEYIQTTIKSEVIGEKFVA
CCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFKINKGQTKPP
CCCEECCCCCCEECCCHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCC
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKE
CCCCCCCEEHHHHCCHHEEECCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
IVPTSKGKQVIDLVPKDLKSPLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVK
CCCCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
NSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRECGYRENVSRFTNVRCPECHV
CCCCCEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEE
KLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVAKYMKKMQKENDEPINS
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
ALAEALSKLKL
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA