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The map label for this gene is potCD [H]

Identifier: 226947815

GI number: 226947815

Start: 723284

End: 725128

Strand: Direct

Name: potCD [H]

Synonym: CLM_0667

Alternate gene names: 226947815

Gene position: 723284-725128 (Clockwise)

Preceding gene: 226947814

Following gene: 226947817

Centisome position: 17.41

GC content: 29.7

Gene sequence:

>1845_bases
ATGATGAAGAGCAATAAAGGTTTATTAAGTAAAATATATGCAACTTTAGTTTATGTTTTTTTATATTTGCCAATCTTTGT
TTTAGTAGTATTTTCTTTTAATAAGTCTAAGTTAAATGCTACCTTTACAGGATTTACATTAGATTGGTACAAAAATTTAA
TAAATAATGTTCAAATATTAGAAGCATTAAAAAACAGCTTAATAATAGCTTTTATAAGTACTTTTTTTGCAGTAATTATA
GGAACTTTAGCTGCTATAGGTATGTATAGATATAAATTTAAGGGGAAAAGGGCTATGGAGGGACTGCTTTATATCCCAGT
AGTTATTCCAGAAATAGTTATGGGAATATCTATGCTTGCCTTTTTCTCTTCCCTTAATTTGCCTGCAGGGCTTATAACTT
TAATACTTGCTCACATAACTTTTTGTATTTCCTACGTTATTATAGTAGTTAGAGCTAGATTAGATGGGTTTGATGCTGCA
CTAGAAGAAGCAGCTCAAGATTTAGGTGCTACACCTTGGCAAACTTTAACGAAGATTACACTACCTGTAATATCACCAGG
AATTATATCAGGAGCACTGCTTGCCTTTACACTATCTTTAGACGATGTTATTATAAGTTTTTTTGCGGCGGGACCAGATA
GTAATACTTTGCCACTTAAAATATTTTCTATGGTTAAGTTTGGTGTAACTCCTGAAATAAATGCACTATCTACTGTGATG
ATGGTATTTACTTTAAGTATGGTAGTTATAGCTGAAGGTATAAGAAGAAATATGCTTAAGAATAAAAAAGTAAAAAAGAC
ATTATCCTTTATAGTAATATTATTAATGGTTACTGGGATGGGCTTTACTATTTTCGGTAATACAGCTAAAACAGAAAAGC
AAGTGTTAAATATATTTAATTGGTCAGAATTTTTACCACAGTCTGTTATTGAACAGTTTGAAAAAGAATACAATGTAAAG
GTAAATTATTCGACTTTTTCCTCTAATGAAGAAATGCTTGCAAAGTTAATGGGAGGAAATGTGCCCTATGATTTAGTAGT
TACCAGTGATTATGCTATTGAAATAATGACTAAACAAAAATTAATTCAACCAATAGACAAAAATAATGTTCCAAACTTAG
CTAATATAGATAAAAATGTACTTGATTTAGCCTTTGATCCTAAAAATACTTATAGCCTACCTTATATGTGGGGAGGCAAC
AATATAGTAATAGATAAGACTAAAATAACTAAAAAAATTACTTCTTTTGATGATCTGTGGGACAGTGAATTTAAAAATTC
TATGGTAATTTTGGATGATCCAAGGGTTATGATAGGATTAGCTCTACAAAAGAATGGATATTCTATAAATTCAAAAAATC
CAAAGGAACTTCAAAAGGCTAAAGAGGATTTAATAAAATTAATGCCAAATGTAAAAGCCTTTGATAGTGAATCTCCAAAG
ACTTTACTTATAAATGGAGAAGCTTCTATAGGATATGTATGGGGAACGGAAGCATATCTTGCTAAACTTGAAAATCCTAA
TTTAGAAGTAGTTTTAACAAAAGAGGGTGTAATTCCTCAATATGATAACTTTGTAATACCTAAAAAGGCTAAAAATAAAA
AATTAGCAGAGGAATTTATAAACTTCATATATAAACCTGAAATAAGTGCTCAAGTGTCTGAGGAATTTCCTTATGCAAAT
CCGAATAAAGCAGCATATCCTTTAATGGATAAAGATAAATTAAATGATATAGCTGTTTATCCACCAAGAGAAGCGGTAGA
AGGAAATGAGCTTATTAAGGATGTAGGGGAAACTACAAAATTATATGATGATATATGGACTGAAATTAAAAATAGTAAAA
GATAA

Upstream 100 bases:

>100_bases
GTTCAGCTATAGCAGTAATATTAATTATTTTAATGCTTATAGCTATAGGTTTTTATTTTAAAGGAAATAAACCAAAGAAG
GGGATAAAGGAGGTTAGCTA

Downstream 100 bases:

>100_bases
CACAAGATTTAAAAATTTTCTAACCAGGTGAGAAATTATCCGTGATTTCACACCTGGTTTCTTATGTTTTGTTAGAAATA
ATAACTGGAAGTATTTATCT

Product: spermidine/putrescine transport system permease PotC/spermidine/putrescine-binding periplasmic protein PotD

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MMKSNKGLLSKIYATLVYVFLYLPIFVLVVFSFNKSKLNATFTGFTLDWYKNLINNVQILEALKNSLIIAFISTFFAVII
GTLAAIGMYRYKFKGKRAMEGLLYIPVVIPEIVMGISMLAFFSSLNLPAGLITLILAHITFCISYVIIVVRARLDGFDAA
LEEAAQDLGATPWQTLTKITLPVISPGIISGALLAFTLSLDDVIISFFAAGPDSNTLPLKIFSMVKFGVTPEINALSTVM
MVFTLSMVVIAEGIRRNMLKNKKVKKTLSFIVILLMVTGMGFTIFGNTAKTEKQVLNIFNWSEFLPQSVIEQFEKEYNVK
VNYSTFSSNEEMLAKLMGGNVPYDLVVTSDYAIEIMTKQKLIQPIDKNNVPNLANIDKNVLDLAFDPKNTYSLPYMWGGN
NIVIDKTKITKKITSFDDLWDSEFKNSMVILDDPRVMIGLALQKNGYSINSKNPKELQKAKEDLIKLMPNVKAFDSESPK
TLLINGEASIGYVWGTEAYLAKLENPNLEVVLTKEGVIPQYDNFVIPKKAKNKKLAEEFINFIYKPEISAQVSEEFPYAN
PNKAAYPLMDKDKLNDIAVYPPREAVEGNELIKDVGETTKLYDDIWTEIKNSKR

Sequences:

>Translated_614_residues
MMKSNKGLLSKIYATLVYVFLYLPIFVLVVFSFNKSKLNATFTGFTLDWYKNLINNVQILEALKNSLIIAFISTFFAVII
GTLAAIGMYRYKFKGKRAMEGLLYIPVVIPEIVMGISMLAFFSSLNLPAGLITLILAHITFCISYVIIVVRARLDGFDAA
LEEAAQDLGATPWQTLTKITLPVISPGIISGALLAFTLSLDDVIISFFAAGPDSNTLPLKIFSMVKFGVTPEINALSTVM
MVFTLSMVVIAEGIRRNMLKNKKVKKTLSFIVILLMVTGMGFTIFGNTAKTEKQVLNIFNWSEFLPQSVIEQFEKEYNVK
VNYSTFSSNEEMLAKLMGGNVPYDLVVTSDYAIEIMTKQKLIQPIDKNNVPNLANIDKNVLDLAFDPKNTYSLPYMWGGN
NIVIDKTKITKKITSFDDLWDSEFKNSMVILDDPRVMIGLALQKNGYSINSKNPKELQKAKEDLIKLMPNVKAFDSESPK
TLLINGEASIGYVWGTEAYLAKLENPNLEVVLTKEGVIPQYDNFVIPKKAKNKKLAEEFINFIYKPEISAQVSEEFPYAN
PNKAAYPLMDKDKLNDIAVYPPREAVEGNELIKDVGETTKLYDDIWTEIKNSKR
>Mature_614_residues
MMKSNKGLLSKIYATLVYVFLYLPIFVLVVFSFNKSKLNATFTGFTLDWYKNLINNVQILEALKNSLIIAFISTFFAVII
GTLAAIGMYRYKFKGKRAMEGLLYIPVVIPEIVMGISMLAFFSSLNLPAGLITLILAHITFCISYVIIVVRARLDGFDAA
LEEAAQDLGATPWQTLTKITLPVISPGIISGALLAFTLSLDDVIISFFAAGPDSNTLPLKIFSMVKFGVTPEINALSTVM
MVFTLSMVVIAEGIRRNMLKNKKVKKTLSFIVILLMVTGMGFTIFGNTAKTEKQVLNIFNWSEFLPQSVIEQFEKEYNVK
VNYSTFSSNEEMLAKLMGGNVPYDLVVTSDYAIEIMTKQKLIQPIDKNNVPNLANIDKNVLDLAFDPKNTYSLPYMWGGN
NIVIDKTKITKKITSFDDLWDSEFKNSMVILDDPRVMIGLALQKNGYSINSKNPKELQKAKEDLIKLMPNVKAFDSESPK
TLLINGEASIGYVWGTEAYLAKLENPNLEVVLTKEGVIPQYDNFVIPKKAKNKKLAEEFINFIYKPEISAQVSEEFPYAN
PNKAAYPLMDKDKLNDIAVYPPREAVEGNELIKDVGETTKLYDDIWTEIKNSKR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=324, Percent_Identity=39.8148148148148, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1787368, Length=254, Percent_Identity=45.2755905511811, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1787081, Length=233, Percent_Identity=44.206008583691, Blast_Score=186, Evalue=6e-48,
Organism=Escherichia coli, GI1787078, Length=329, Percent_Identity=31.6109422492401, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1787714, Length=205, Percent_Identity=38.5365853658537, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788764, Length=179, Percent_Identity=30.1675977653631, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 68841; Mature: 68841

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKSNKGLLSKIYATLVYVFLYLPIFVLVVFSFNKSKLNATFTGFTLDWYKNLINNVQIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECEEHHHHHHHHHHHHHH
EALKNSLIIAFISTFFAVIIGTLAAIGMYRYKFKGKRAMEGLLYIPVVIPEIVMGISMLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEHHHHHHHHHHHHHHHH
FFSSLNLPAGLITLILAHITFCISYVIIVVRARLDGFDAALEEAAQDLGATPWQTLTKIT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHH
LPVISPGIISGALLAFTLSLDDVIISFFAAGPDSNTLPLKIFSMVKFGVTPEINALSTVM
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
MVFTLSMVVIAEGIRRNMLKNKKVKKTLSFIVILLMVTGMGFTIFGNTAKTEKQVLNIFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
WSEFLPQSVIEQFEKEYNVKVNYSTFSSNEEMLAKLMGGNVPYDLVVTSDYAIEIMTKQK
HHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCEEEEEHHHH
LIQPIDKNNVPNLANIDKNVLDLAFDPKNTYSLPYMWGGNNIVIDKTKITKKITSFDDLW
HHCCCCCCCCCCHHHCCCHHHEEEECCCCCEECCEEECCCEEEEECHHHHHHHHHHHHHH
DSEFKNSMVILDDPRVMIGLALQKNGYSINSKNPKELQKAKEDLIKLMPNVKAFDSESPK
HHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
TLLINGEASIGYVWGTEAYLAKLENPNLEVVLTKEGVIPQYDNFVIPKKAKNKKLAEEFI
EEEEECCCCEEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHH
NFIYKPEISAQVSEEFPYANPNKAAYPLMDKDKLNDIAVYPPREAVEGNELIKDVGETTK
HHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHH
LYDDIWTEIKNSKR
HHHHHHHHHHCCCC
>Mature Secondary Structure
MMKSNKGLLSKIYATLVYVFLYLPIFVLVVFSFNKSKLNATFTGFTLDWYKNLINNVQIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECEEHHHHHHHHHHHHHH
EALKNSLIIAFISTFFAVIIGTLAAIGMYRYKFKGKRAMEGLLYIPVVIPEIVMGISMLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEHHHHHHHHHHHHHHHH
FFSSLNLPAGLITLILAHITFCISYVIIVVRARLDGFDAALEEAAQDLGATPWQTLTKIT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHH
LPVISPGIISGALLAFTLSLDDVIISFFAAGPDSNTLPLKIFSMVKFGVTPEINALSTVM
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
MVFTLSMVVIAEGIRRNMLKNKKVKKTLSFIVILLMVTGMGFTIFGNTAKTEKQVLNIFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
WSEFLPQSVIEQFEKEYNVKVNYSTFSSNEEMLAKLMGGNVPYDLVVTSDYAIEIMTKQK
HHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCEEEEEHHHH
LIQPIDKNNVPNLANIDKNVLDLAFDPKNTYSLPYMWGGNNIVIDKTKITKKITSFDDLW
HHCCCCCCCCCCHHHCCCHHHEEEECCCCCEECCEEECCCEEEEECHHHHHHHHHHHHHH
DSEFKNSMVILDDPRVMIGLALQKNGYSINSKNPKELQKAKEDLIKLMPNVKAFDSESPK
HHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
TLLINGEASIGYVWGTEAYLAKLENPNLEVVLTKEGVIPQYDNFVIPKKAKNKKLAEEFI
EEEEECCCCEEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHH
NFIYKPEISAQVSEEFPYANPNKAAYPLMDKDKLNDIAVYPPREAVEGNELIKDVGETTK
HHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHH
LYDDIWTEIKNSKR
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]