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The map label for this gene is potB [H]

Identifier: 226947814

GI number: 226947814

Start: 722325

End: 723284

Strand: Direct

Name: potB [H]

Synonym: CLM_0666

Alternate gene names: 226947814

Gene position: 722325-723284 (Clockwise)

Preceding gene: 226947813

Following gene: 226947815

Centisome position: 17.38

GC content: 29.17

Gene sequence:

>960_bases
ATGTGGGGGAAATTTAGCAGTGCTTTTTCAAATATTTTTAAAAAGCAATTGCATAATAAAAATACAGATATTATTTTAGA
AGACAAAGAAGGAATAAGAGGTAAACTAAAAAAATCTACCCCTATATTTAAAACAGTGGGGCCCGTTGCCCTATGGCTCA
TTACATTTTTTATTGTACCTTTACTTCTTGTATTAGTGGTTAGTTTTCTATCAAGAGGTGAATACGGAGATGTAAGATTA
CCTTTTACGCTAAAAAATTATAAAAGACTCATGGATCCTATGTACGGACAAATTTTAATTAAAACTTTAATAATTTCTAT
TAGTACTACTTTAGGGTGTTTGATCATAGGTTATCCCTTTGCATATTTTATTGGAAGATCAGATAAAAAACATAGAGGAA
TACTATTATTATTAGTTATACTACCTTTTTGGACTAATTCTTTAGTTCGAACTTATGCATGGATAATACTTTTAAAAACA
GAGGGAGTAATTAACACATATCTTATGAAATTTGGATTAATTCACTCTCCATTGCAAATGCTTTATACAGATGGTGCTGT
ATTTATAGGAATGCTTTATATAATGTTACCTTTTATGGTGCTACCTATTTATACATCTATAGAAAAATTAGATATGAGTT
ATATAGAAGCTGCCAATGATCTTGGAGCATCGCCTTTTAAAACCTTTAAAGAAATAACATTGCCTTTAACTCTATCGGGT
ATTATAGCAGGATGTATGCTTGTATTTATACCAACTTTAGGTTATTTCTTCATATCTGATTTAATGGGTGGAAGTAAAAC
TATGTTAATTTCAAATCTAATCAAAAATCAATTTTTAACCTCTAGAGATTGGCCCTTTGGTTCAGCTATAGCAGTAATAT
TAATTATTTTAATGCTTATAGCTATAGGTTTTTATTTTAAAGGAAATAAACCAAAGAAGGGGATAAAGGAGGTTAGCTAA

Upstream 100 bases:

>100_bases
TGGTGTTAGAGCCTATAAGTGATGTATATTCTCTAGATACCCATAATAAAAACTATGTGTTTGCCACATGGAATCCTAAA
CATGCAGTGGTGATGCATTA

Downstream 100 bases:

>100_bases
TGATGAAGAGCAATAAAGGTTTATTAAGTAAAATATATGCAACTTTAGTTTATGTTTTTTTATATTTGCCAATCTTTGTT
TTAGTAGTATTTTCTTTTAA

Product: spermidine/putrescine ABC transporter permease PotB

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MWGKFSSAFSNIFKKQLHNKNTDIILEDKEGIRGKLKKSTPIFKTVGPVALWLITFFIVPLLLVLVVSFLSRGEYGDVRL
PFTLKNYKRLMDPMYGQILIKTLIISISTTLGCLIIGYPFAYFIGRSDKKHRGILLLLVILPFWTNSLVRTYAWIILLKT
EGVINTYLMKFGLIHSPLQMLYTDGAVFIGMLYIMLPFMVLPIYTSIEKLDMSYIEAANDLGASPFKTFKEITLPLTLSG
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLIAIGFYFKGNKPKKGIKEVS

Sequences:

>Translated_319_residues
MWGKFSSAFSNIFKKQLHNKNTDIILEDKEGIRGKLKKSTPIFKTVGPVALWLITFFIVPLLLVLVVSFLSRGEYGDVRL
PFTLKNYKRLMDPMYGQILIKTLIISISTTLGCLIIGYPFAYFIGRSDKKHRGILLLLVILPFWTNSLVRTYAWIILLKT
EGVINTYLMKFGLIHSPLQMLYTDGAVFIGMLYIMLPFMVLPIYTSIEKLDMSYIEAANDLGASPFKTFKEITLPLTLSG
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLIAIGFYFKGNKPKKGIKEVS
>Mature_319_residues
MWGKFSSAFSNIFKKQLHNKNTDIILEDKEGIRGKLKKSTPIFKTVGPVALWLITFFIVPLLLVLVVSFLSRGEYGDVRL
PFTLKNYKRLMDPMYGQILIKTLIISISTTLGCLIIGYPFAYFIGRSDKKHRGILLLLVILPFWTNSLVRTYAWIILLKT
EGVINTYLMKFGLIHSPLQMLYTDGAVFIGMLYIMLPFMVLPIYTSIEKLDMSYIEAANDLGASPFKTFKEITLPLTLSG
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLIAIGFYFKGNKPKKGIKEVS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=259, Percent_Identity=49.034749034749, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1787080, Length=244, Percent_Identity=45.0819672131148, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1787713, Length=236, Percent_Identity=33.0508474576271, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36004; Mature: 36004

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]