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The map label for this gene is glvA [H]

Identifier: 226947502

GI number: 226947502

Start: 323107

End: 324432

Strand: Direct

Name: glvA [H]

Synonym: CLM_0336

Alternate gene names: 226947502

Gene position: 323107-324432 (Clockwise)

Preceding gene: 226947501

Following gene: 226947503

Centisome position: 7.78

GC content: 31.9

Gene sequence:

>1326_bases
ATGAAAAAATTCTCAGTAACAATAGCTGGTGGTGGAAGTACATTTACGCCAGGAATAATATTAATGCTGTTAGATAACTT
GGATAAATTTCCTATAAAAAAATTAAAGCTATATGATAATGATAAAGAAAGACAAGAAATTGTTGCTGGAGCTTGTAAGG
TTATTTTAAAAGAGAAAGCACCAGAAATTGAATTTTTAGCTACAGTAGATCCAGAGGAAGCTTTTACAGATATAGATTTT
GTTATGGCTCATATAAGAGTAGGTAAATATGCAATGCGTGAATTAGATGAAAAAATTCCTTTAAAATATGATGTACTTGG
ACAAGAAACTTGCGGTCCAGGAGGAATGGCTTATGGTATGAGATCTATTGGTGGAGTTATTGAAATACTTGATTATATGG
AAAAATATTCACCAAATGCATGGATGCTAAATTACTCTAATCCAGCAGCAATAGTTGCAGAGGCCACTAGAAGATTAAGA
CCTAATTCAAAGATATTGAACATATGTGATATGCCAATAGGTATAGAAGTGCGTATGGCTGAAATTTTAGGATTAGAGTC
AAGAAAGGATATGTCAGTTCGCTATTACGGACTTAATCATTTTGGTTGGTGGACAGACATACGTGACAAAGAAGGAAATG
ACTTAATGCCAAAAATAAAAGAACACGTTTCTAAACATGGATATGTGGTTGATAAGGGAGATAGTCAACATACTGAAGCA
AGTTGGAATGACACCTTTGCTAAAGCAAAAGATGTATATGCTGTTGATCCAAATACATTACCTAATACTTATTTAAAATA
TTATTTATTCCAAGATTATGTTGTTAAACATGCTAATAAAGAATATACAAGAGCAAATGAAGTAATGGATGGAAGAGAAA
AGTTTGTATTTGGAGAATGTAAAAAAGTTATAGAAAAACAATCTACAGAAGGTTGTGAACTTCACATAGACGAACATGCT
TGTTATATAGTTGATCTTGCAAGAGCTATAGCATACAATACAAAGGAAAGAATGTTGTTAATTGTGGAAAATAATGGTAT
AATTGAAAATTTTGATTCTACAGCTATGGTTGAAGTACCTTGTATTCTTGGTAGTAATGGACCAGAGCCATTAGCAATAG
GTAAGATTCCACAATTCCAAAAAGGATTAATGGAACAACAAGTTTCTGTAGAAAAATTAGTAGTTGAAGCTTGGATAGAA
AAATCTTATCAAAAATTATGGCAAGCTATTACACTTTCTAAGACAGTACCTAGTGCATCCGTTGCTAAGAATATTTTAGA
TGATTTAATTGAAGCAAATAAGGATTATTGGCCTGAATTAAAGTAG

Upstream 100 bases:

>100_bases
TCAAAACAAATGAAGTTAAGGATGTAGTCAAAACAACTGATGGTAAATAATTTATAATATGAAAATAAATACAAAATAAA
ATTAGAAAGAAGGAATAACT

Downstream 100 bases:

>100_bases
TATTTAAAGATAATTTAATAATATTATTTTAAAAAATAGAAAAATTATTGTAAAATTAATTGAGACTGCCAAAAGTAGAT
TAAAACCTATTTTAGACAGT

Product: maltose-6'-phosphate glucosidase

Products: NA

Alternate protein names: 6-phospho-alpha-glucosidase; 6-phospho-glucosidase; Maltose-6-phosphate hydrolase [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKKFSVTIAGGGSTFTPGIILMLLDNLDKFPIKKLKLYDNDKERQEIVAGACKVILKEKAPEIEFLATVDPEEAFTDIDF
VMAHIRVGKYAMRELDEKIPLKYDVLGQETCGPGGMAYGMRSIGGVIEILDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
PNSKILNICDMPIGIEVRMAEILGLESRKDMSVRYYGLNHFGWWTDIRDKEGNDLMPKIKEHVSKHGYVVDKGDSQHTEA
SWNDTFAKAKDVYAVDPNTLPNTYLKYYLFQDYVVKHANKEYTRANEVMDGREKFVFGECKKVIEKQSTEGCELHIDEHA
CYIVDLARAIAYNTKERMLLIVENNGIIENFDSTAMVEVPCILGSNGPEPLAIGKIPQFQKGLMEQQVSVEKLVVEAWIE
KSYQKLWQAITLSKTVPSASVAKNILDDLIEANKDYWPELK

Sequences:

>Translated_441_residues
MKKFSVTIAGGGSTFTPGIILMLLDNLDKFPIKKLKLYDNDKERQEIVAGACKVILKEKAPEIEFLATVDPEEAFTDIDF
VMAHIRVGKYAMRELDEKIPLKYDVLGQETCGPGGMAYGMRSIGGVIEILDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
PNSKILNICDMPIGIEVRMAEILGLESRKDMSVRYYGLNHFGWWTDIRDKEGNDLMPKIKEHVSKHGYVVDKGDSQHTEA
SWNDTFAKAKDVYAVDPNTLPNTYLKYYLFQDYVVKHANKEYTRANEVMDGREKFVFGECKKVIEKQSTEGCELHIDEHA
CYIVDLARAIAYNTKERMLLIVENNGIIENFDSTAMVEVPCILGSNGPEPLAIGKIPQFQKGLMEQQVSVEKLVVEAWIE
KSYQKLWQAITLSKTVPSASVAKNILDDLIEANKDYWPELK
>Mature_441_residues
MKKFSVTIAGGGSTFTPGIILMLLDNLDKFPIKKLKLYDNDKERQEIVAGACKVILKEKAPEIEFLATVDPEEAFTDIDF
VMAHIRVGKYAMRELDEKIPLKYDVLGQETCGPGGMAYGMRSIGGVIEILDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
PNSKILNICDMPIGIEVRMAEILGLESRKDMSVRYYGLNHFGWWTDIRDKEGNDLMPKIKEHVSKHGYVVDKGDSQHTEA
SWNDTFAKAKDVYAVDPNTLPNTYLKYYLFQDYVVKHANKEYTRANEVMDGREKFVFGECKKVIEKQSTEGCELHIDEHA
CYIVDLARAIAYNTKERMLLIVENNGIIENFDSTAMVEVPCILGSNGPEPLAIGKIPQFQKGLMEQQVSVEKLVVEAWIE
KSYQKLWQAITLSKTVPSASVAKNILDDLIEANKDYWPELK

Specific function: Catalyzes the hydrolysis of O-alpha-linked disaccharide 6-phosphates, including maltose-6'P and all five phosphorylated isomers of sucrose, but not sucrose-6P. Does not hydrolyze beta- linked disaccharide 6-phosphates such as cellobiose-6'P and gentiobios

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=459, Percent_Identity=27.2331154684096, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1790560, Length=468, Percent_Identity=22.008547008547, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.122 [H]

Molecular weight: Translated: 50007; Mature: 50007

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFSVTIAGGGSTFTPGIILMLLDNLDKFPIKKLKLYDNDKERQEIVAGACKVILKEKA
CCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCC
PEIEFLATVDPEEAFTDIDFVMAHIRVGKYAMRELDEKIPLKYDVLGQETCGPGGMAYGM
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHH
RSIGGVIEILDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPNSKILNICDMPIGIEVRMA
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEHH
EILGLESRKDMSVRYYGLNHFGWWTDIRDKEGNDLMPKIKEHVSKHGYVVDKGDSQHTEA
HHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCC
SWNDTFAKAKDVYAVDPNTLPNTYLKYYLFQDYVVKHANKEYTRANEVMDGREKFVFGEC
CCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHH
KKVIEKQSTEGCELHIDEHACYIVDLARAIAYNTKERMLLIVENNGIIENFDSTAMVEVP
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCEEEEEE
CILGSNGPEPLAIGKIPQFQKGLMEQQVSVEKLVVEAWIEKSYQKLWQAITLSKTVPSAS
EEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VAKNILDDLIEANKDYWPELK
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKFSVTIAGGGSTFTPGIILMLLDNLDKFPIKKLKLYDNDKERQEIVAGACKVILKEKA
CCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCC
PEIEFLATVDPEEAFTDIDFVMAHIRVGKYAMRELDEKIPLKYDVLGQETCGPGGMAYGM
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHH
RSIGGVIEILDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPNSKILNICDMPIGIEVRMA
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEHH
EILGLESRKDMSVRYYGLNHFGWWTDIRDKEGNDLMPKIKEHVSKHGYVVDKGDSQHTEA
HHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCC
SWNDTFAKAKDVYAVDPNTLPNTYLKYYLFQDYVVKHANKEYTRANEVMDGREKFVFGEC
CCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHH
KKVIEKQSTEGCELHIDEHACYIVDLARAIAYNTKERMLLIVENNGIIENFDSTAMVEVP
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCEEEEEE
CILGSNGPEPLAIGKIPQFQKGLMEQQVSVEKLVVEAWIEKSYQKLWQAITLSKTVPSAS
EEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VAKNILDDLIEANKDYWPELK
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11781805; 11466286 [H]