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The map label for this gene is crr [H]

Identifier: 226947496

GI number: 226947496

Start: 315476

End: 315958

Strand: Direct

Name: crr [H]

Synonym: CLM_0330

Alternate gene names: 226947496

Gene position: 315476-315958 (Clockwise)

Preceding gene: 226947493

Following gene: 226947497

Centisome position: 7.59

GC content: 28.57

Gene sequence:

>483_bases
ATGTTTTCATTATTTAAAAAGAAAGAAAAAAATTTAAAATTAAAAGCTTATTTATCAGGAAAGATGATTTCAATTAATAA
AGTACCAGATGAGGTATTTTCATCAAAAATCATGGGAGATGGATTAGCAATTATTCCAACTACAAGTATAGTAAAATCAC
CAGCAGATGGTGAAATTACAGTAGTCATGGAGGAGTCTAAACATGCAGTTGGAATTAAATTCGAAAATGGAGTAGAAGCA
TTGATTCATGTTGGAATTGATACAGTCTCTATGAATGGAGAAGGGTTTGAAGTTTTTGTGAAAACAGGAGATTCAGTAAA
AGAAGGACAAGATCTTATTATGTTTGATGAAGAATTAATTAAAAATAGAGGCTTATCAACATATACTATGTTAGTAATAA
CTAATTCTTCTGAGTATCCTAATATGGTCATAGAAACAGAAAAAGAGGTTAAGGAAAAAGAAAGTGTAATTATAACTTTC
TAA

Upstream 100 bases:

>100_bases
TTAATGATTAGGGTCGTTATTGTTTCCCCAAAAATTTTACAAAAATAATTTTTTAATAGAACTATTATAATAAAATAAAT
TATATTCTAGGAGGTTAAAT

Downstream 100 bases:

>100_bases
TAAAATTATGTTATTAATATTTAGATATGTAGATTTAAATAAAGGCAATTTTGTTCATAATTACTCCTCTAAATTTTATT
TAGAGGAGATTTTTCTATTG

Product: PTS system glucose family Etransporter subunit IIA

Products: Protein Histidine; Sugar Phosphate.; glucose-6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MFSLFKKKEKNLKLKAYLSGKMISINKVPDEVFSSKIMGDGLAIIPTTSIVKSPADGEITVVMEESKHAVGIKFENGVEA
LIHVGIDTVSMNGEGFEVFVKTGDSVKEGQDLIMFDEELIKNRGLSTYTMLVITNSSEYPNMVIETEKEVKEKESVIITF

Sequences:

>Translated_160_residues
MFSLFKKKEKNLKLKAYLSGKMISINKVPDEVFSSKIMGDGLAIIPTTSIVKSPADGEITVVMEESKHAVGIKFENGVEA
LIHVGIDTVSMNGEGFEVFVKTGDSVKEGQDLIMFDEELIKNRGLSTYTMLVITNSSEYPNMVIETEKEVKEKESVIITF
>Mature_160_residues
MFSLFKKKEKNLKLKAYLSGKMISINKVPDEVFSSKIMGDGLAIIPTTSIVKSPADGEITVVMEESKHAVGIKFENGVEA
LIHVGIDTVSMNGEGFEVFVKTGDSVKEGQDLIMFDEELIKNRGLSTYTMLVITNSSEYPNMVIETEKEVKEKESVIITF

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=138, Percent_Identity=41.304347826087, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1790159, Length=140, Percent_Identity=35, Blast_Score=99, Evalue=1e-22,
Organism=Escherichia coli, GI1786894, Length=138, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 17716; Mature: 17716

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSLFKKKEKNLKLKAYLSGKMISINKVPDEVFSSKIMGDGLAIIPTTSIVKSPADGEIT
CCCCCCCCCCCEEEEEEECCEEEEEECCCHHHHHHHHCCCCEEEEECHHHHCCCCCCEEE
VVMEESKHAVGIKFENGVEALIHVGIDTVSMNGEGFEVFVKTGDSVKEGQDLIMFDEELI
EEEECCCCEEEEEECCCCEEEEEECCEEEEECCCCEEEEEECCCCCCCCCEEEEECHHHH
KNRGLSTYTMLVITNSSEYPNMVIETEKEVKEKESVIITF
HHCCCCEEEEEEEECCCCCCCEEEECHHHHHCCCCEEEEC
>Mature Secondary Structure
MFSLFKKKEKNLKLKAYLSGKMISINKVPDEVFSSKIMGDGLAIIPTTSIVKSPADGEIT
CCCCCCCCCCCEEEEEEECCEEEEEECCCHHHHHHHHCCCCEEEEECHHHHCCCCCCEEE
VVMEESKHAVGIKFENGVEALIHVGIDTVSMNGEGFEVFVKTGDSVKEGQDLIMFDEELI
EEEECCCCEEEEEECCCCEEEEEECCEEEEECCCCEEEEEECCCCCCCCCEEEEECHHHH
KNRGLSTYTMLVITNSSEYPNMVIETEKEVKEKESVIITF
HHCCCCEEEEEEEECCCCCCCEEEECHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar; phosphoenolpyruvate; beta-D-glucose [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm] [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytop

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA