Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 226947282

GI number: 226947282

Start: 83551

End: 84495

Strand: Direct

Name: holB [H]

Synonym: CLM_0099

Alternate gene names: 226947282

Gene position: 83551-84495 (Clockwise)

Preceding gene: 226947281

Following gene: 226947283

Centisome position: 2.01

GC content: 22.65

Gene sequence:

>945_bases
TTGGATTCACATACAATAATAGGGCATGAAAATATAAAAAATAGGATTAAGAATTCAATAATTAAAGGTAAGTTTCCTCA
TGCCAGTATTATTGTTGGAGAGGATGGCATAGGTAAAAGTATAATAGCTAAAGAAATGGCTATGATTATATTAGAAAAAT
CACAGCAAAGAAGCTATGCAGACCTAATAGAATTTAAACCTATTAATAAAAAATCTATAGGTATAGATGATATAAGAAAC
TTAATAGAAGAAATAAATAAAAAACCAATAGAAGGAAATAAAAAAGTTATTATAATATATAAATCTGAATTAATAACAGA
AGTTGCTCAAAATGCTTTTTTAAAAACTATAGAGGAGCCACCTAAGGGTGTTTATATAATATTATTATGTGAAAATATGG
AACAAATGTTGGATACTATAAAATCTAGATGTGAAATATTTAAATTAAATAGATTAAAGCCATGTGATATAGAAAATTTT
ATAAAATATAAATACAAAGATATAAAAAAAGAAAATATAAATTCAGCAATAGCATTTAGCGATGGAATTCCAGGTAAAGC
TGAAAGATTTATTGAAGATGAATCATTAAAATATATAAGAGATATGAGCTTGGGTATATTAAAAGATTCAAAAGATTTAA
GCAATTATAATTATTTGATAAAATATGAAAATTTTTTAATAAATTATAAAGAAAATTGGGAAGAGATTTTAACTTGTATT
TTATCATATCTTAGAGATTCATTATTATTTAAAGAAACTGGAGATGAAGAGTTATTATTAAATGTAGATAAGAAAGAGTA
TATAAAACATATATCAGAAAAATATTCATATATAAACTTAGATAAAATGGTAAGTATAATAAATGATACAAGAGATAAAT
TAAGTAAAAATATAAACTCCACTCTAGTATTTGATGCAATGCTTATAAAAATGCAGGAGGTATAA

Upstream 100 bases:

>100_bases
TGTACGTATCTTATCCAATAGAAGTAGAGGTTGGAGGAGCTACTATATTTGTAGTGGATGTAGACAAATTTGCAAGAGTT
TAATTTTAGGAGGGATACAT

Downstream 100 bases:

>100_bases
TATGATAGAAGTTGTGGGAGTCCGATTTAAAAAGGCAGGTAAAATATATTATTTTGATCCTAGTAATATAGAAATAAATA
AGGGAGAATATGTTATAGTA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MDSHTIIGHENIKNRIKNSIIKGKFPHASIIVGEDGIGKSIIAKEMAMIILEKSQQRSYADLIEFKPINKKSIGIDDIRN
LIEEINKKPIEGNKKVIIIYKSELITEVAQNAFLKTIEEPPKGVYIILLCENMEQMLDTIKSRCEIFKLNRLKPCDIENF
IKYKYKDIKKENINSAIAFSDGIPGKAERFIEDESLKYIRDMSLGILKDSKDLSNYNYLIKYENFLINYKENWEEILTCI
LSYLRDSLLFKETGDEELLLNVDKKEYIKHISEKYSYINLDKMVSIINDTRDKLSKNINSTLVFDAMLIKMQEV

Sequences:

>Translated_314_residues
MDSHTIIGHENIKNRIKNSIIKGKFPHASIIVGEDGIGKSIIAKEMAMIILEKSQQRSYADLIEFKPINKKSIGIDDIRN
LIEEINKKPIEGNKKVIIIYKSELITEVAQNAFLKTIEEPPKGVYIILLCENMEQMLDTIKSRCEIFKLNRLKPCDIENF
IKYKYKDIKKENINSAIAFSDGIPGKAERFIEDESLKYIRDMSLGILKDSKDLSNYNYLIKYENFLINYKENWEEILTCI
LSYLRDSLLFKETGDEELLLNVDKKEYIKHISEKYSYINLDKMVSIINDTRDKLSKNINSTLVFDAMLIKMQEV
>Mature_314_residues
MDSHTIIGHENIKNRIKNSIIKGKFPHASIIVGEDGIGKSIIAKEMAMIILEKSQQRSYADLIEFKPINKKSIGIDDIRN
LIEEINKKPIEGNKKVIIIYKSELITEVAQNAFLKTIEEPPKGVYIILLCENMEQMLDTIKSRCEIFKLNRLKPCDIENF
IKYKYKDIKKENINSAIAFSDGIPGKAERFIEDESLKYIRDMSLGILKDSKDLSNYNYLIKYENFLINYKENWEEILTCI
LSYLRDSLLFKETGDEELLLNVDKKEYIKHISEKYSYINLDKMVSIINDTRDKLSKNINSTLVFDAMLIKMQEV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=282, Percent_Identity=23.4042553191489, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324478, Length=156, Percent_Identity=28.2051282051282, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI17647857, Length=150, Percent_Identity=28, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36495; Mature: 36495

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSHTIIGHENIKNRIKNSIIKGKFPHASIIVGEDGIGKSIIAKEMAMIILEKSQQRSYA
CCCCEEECHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHH
DLIEFKPINKKSIGIDDIRNLIEEINKKPIEGNKKVIIIYKSELITEVAQNAFLKTIEEP
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
PKGVYIILLCENMEQMLDTIKSRCEIFKLNRLKPCDIENFIKYKYKDIKKENINSAIAFS
CCCEEEEEEECCHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEC
DGIPGKAERFIEDESLKYIRDMSLGILKDSKDLSNYNYLIKYENFLINYKENWEEILTCI
CCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECEEEECCCCHHHHHHHH
LSYLRDSLLFKETGDEELLLNVDKKEYIKHISEKYSYINLDKMVSIINDTRDKLSKNINS
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
TLVFDAMLIKMQEV
HHHHHHHHHHHHCC
>Mature Secondary Structure
MDSHTIIGHENIKNRIKNSIIKGKFPHASIIVGEDGIGKSIIAKEMAMIILEKSQQRSYA
CCCCEEECHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHH
DLIEFKPINKKSIGIDDIRNLIEEINKKPIEGNKKVIIIYKSELITEVAQNAFLKTIEEP
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
PKGVYIILLCENMEQMLDTIKSRCEIFKLNRLKPCDIENFIKYKYKDIKKENINSAIAFS
CCCEEEEEEECCHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEC
DGIPGKAERFIEDESLKYIRDMSLGILKDSKDLSNYNYLIKYENFLINYKENWEEILTCI
CCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECEEEECCCCHHHHHHHH
LSYLRDSLLFKETGDEELLLNVDKKEYIKHISEKYSYINLDKMVSIINDTRDKLSKNINS
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
TLVFDAMLIKMQEV
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430; 10491164 [H]