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The map label for this gene is gyrA [H]

Identifier: 226947229

GI number: 226947229

Start: 6499

End: 9039

Strand: Direct

Name: gyrA [H]

Synonym: CLM_0007

Alternate gene names: 226947229

Gene position: 6499-9039 (Clockwise)

Preceding gene: 226947228

Following gene: 226947230

Centisome position: 0.16

GC content: 29.16

Gene sequence:

>2541_bases
ATGCTAAACGAAGGAAAGATTTTGCCTGTAGATGTAAGTAAAGAAATGAAGAAATGTTATATAGACTATGCCATGAGTGT
AATAGCTGGCCGTGCATTACCAGATGTAAGAGATGGGTTAAAGCCAGTACATAGAAGAATAATATATTCAATGCAGGGAT
TAGGATTAGCTCCAGAAAAAGGTTATAGAAAATGTGCGAGAATTGTAGGGGATGTACTAGGTAAGTATCATCCTCATGGA
GATACAGCAGTTTATGAAGCTTTAGTAAGAATGGCTCAGAATTTTTCAATAAGATATACATTAGTAGATGGTCATGGAAA
TTTTGGTTCTGTAGATGGTGATGGAGCAGCAGCAATGAGATATACAGAAGCTAAGATGAGTAAAATATCTATGGAACTTA
TAAAAGACATAAATAAAAATACAGTTGATTTTATTCCTAACTTTGATGGGGAAGAAGAAGAACCATCTGTATTACCATCA
AGGTTTCCTAATCTTTTAGTTAATGGTTCTTCAGGTATAGCAGTAGGTATGGCTACTAATATTCCACCTCATAATTTAAC
AGAGGTTATAGATGGAATAATAATGCTAATAGAAAATGAAGATGTAAATATACTTGATTTAATGACTAAAATAAAAGGAC
CAGATTTCCCTACTTCTGGATTAATCGTAGGTACAAGAGGAATAAGGGAAGCTTATGAAACTGGTAGAGGAAAAGTTATA
ATTAGAGCTAAAGCTGAGATTGAAGAGGAAAAAGGGAAAAATAAGATAATAGTTACAGAAATACCTTATCAAGTTAATAA
AGCTAGACTTATAGAAAATATGGCTAACCTTGTAAAGGATAAAAAAATAAATGGAATTTCAGATTTAAGAGATGAATCAG
ATAGAGATGGTATGAGAATTGTTATAGAATTAAAAAGGGATGCAAATCCTAATATAGTTTTAAACCAATTATATAAGCAT
ACAAAACTTCAAGATACCTTTGGAATAATCATGTTAGCTTTAGTTAATAACCAACCTCAAATTTTAAATTTAAAAGAAAT
ATTAGTTAATTATGTGGAATTCCAAAAGGAAGTAATAAGAAGAAGAACTAGATTTGACTTAGATAAAGCATTAGCAAGGG
CTCACATATTAGAAGGTTTAAGAATAGCCTTAGATCATATTGATGAAGTTATAAAACTTATAAGAGCATCTAAAAATACA
GCTGAAGCTAAAGAAGGATTAATGAATAACTTTAATCTTTCAGAAAAACAAGCTCAAGCTATATTAGATATGAAGTTACA
AAGGCTTACAGGTTTAGAAAGAGAAAAAATAGAAGAAGAATATAAAGAACTTATGGAAAAGATAAGTTATTTTAGAGAAA
TATTAGATAAAGAAGAATTAGTACTAAGTATAATAAAAGAAGAATTAATAGAGATAAAAAATAAATATGGTGATGAAAGA
AAAACAGAAATAGTAAAAGGTGAACATGATATAGACATAGAGGACTTAATTGAAGATAAAAAGGTGATAGTAACTTTAAC
TCATGGTGGATACATAAAAAGATTAGATATGGATACATACTCTTCACAAAAAAGAGGAGGAAAGGGTATTCAAGCTACAT
CTACAAAACAGGATGATTTTATAGAAAATATGTTTGTAACATCTACTCATAGTACTATATTATTCTTTACTAATAGGGGT
AAGGTATATAAACTTAAAGCTTATGAAATACCAGAAGCAGGAAGAACAGCTAAAGGTACAAATATAGTAAATCTTATACC
TATAGAAAATAATGAGAAAATACAAACAGTAATAGGTTTAAAAGACATAGATGACATGAAGCATTTTGTAATGTGTACGA
GAAATGGAATAATTAAAAAGACAGAGATAAGTAAATATTCTTCCATAAGAAAAGGTGGATTAAATGCCATAAATTTAAGA
GAAGATGATGAATTAATAGATGTAAAAATGACCAAAGGAAATGATGAAATAATAGTAGTAACACAAAATGGGTACTGTAT
AAGATTTAATGAAGAAGATGTAAGACCTATGGGAAGAGTTGCAACAGGTGTTAAGGCTATAACACTAAGAAAAACTGATA
AGGCTGTATCTATGGATGTAGTAATAGAAGATGAAACACTATTATCTATAAGTGAAAATGGATTTGGTAAACGAACAGAC
ATAGAAGAATATCCTATCCATAGAAGAGGTGGAAAAGGTGTTATAACTTATAAGATAACTGACAAAACAGGACCTATAGT
TGGAGCAAGGTTTGTAAAAGAAGATGATGAACTTATGCTTGTAAATAGTGGTGATGTTGCAATAAGAATAAATGTTTCGG
AAATATCTAAAACTAGTAGAAATGCTATGGGTGTAAAACTAATGAGAACTAGTGAAGAAGAAAAAATAGTAGCTATAGCT
AAAATAAAGAGTGAAGACATAATAGAAGAAGAAATTTTAAATGAAGAAAATCTAAATGAAGAAAATCTAAATGAAGAAAA
TCTAAATGAAGAAAATCTAAATGAAGAAAATCTAAATGAAGAAAATCTAAATGAAGAATAA

Upstream 100 bases:

>100_bases
ATGGGAACAAAAGTAGAACCAAGAAGAGACTTTATAGAAAGTAATGCTGATAAAGTAGTTAACTTGGATATATAAGATAA
GATTGTAAAGAGGTGTAATC

Downstream 100 bases:

>100_bases
ATAATAAGAAAAATCAGCATAAATGCTGATTTTTCTTATTATAAATTTTAATTATAAAAACAACTTTTCCACACGTATCA
ATATAAGAAATATCAAAAAA

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 846; Mature: 846

Protein sequence:

>846_residues
MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVHRRIIYSMQGLGLAPEKGYRKCARIVGDVLGKYHPHG
DTAVYEALVRMAQNFSIRYTLVDGHGNFGSVDGDGAAAMRYTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPS
RFPNLLVNGSSGIAVGMATNIPPHNLTEVIDGIIMLIENEDVNILDLMTKIKGPDFPTSGLIVGTRGIREAYETGRGKVI
IRAKAEIEEEKGKNKIIVTEIPYQVNKARLIENMANLVKDKKINGISDLRDESDRDGMRIVIELKRDANPNIVLNQLYKH
TKLQDTFGIIMLALVNNQPQILNLKEILVNYVEFQKEVIRRRTRFDLDKALARAHILEGLRIALDHIDEVIKLIRASKNT
AEAKEGLMNNFNLSEKQAQAILDMKLQRLTGLEREKIEEEYKELMEKISYFREILDKEELVLSIIKEELIEIKNKYGDER
KTEIVKGEHDIDIEDLIEDKKVIVTLTHGGYIKRLDMDTYSSQKRGGKGIQATSTKQDDFIENMFVTSTHSTILFFTNRG
KVYKLKAYEIPEAGRTAKGTNIVNLIPIENNEKIQTVIGLKDIDDMKHFVMCTRNGIIKKTEISKYSSIRKGGLNAINLR
EDDELIDVKMTKGNDEIIVVTQNGYCIRFNEEDVRPMGRVATGVKAITLRKTDKAVSMDVVIEDETLLSISENGFGKRTD
IEEYPIHRRGGKGVITYKITDKTGPIVGARFVKEDDELMLVNSGDVAIRINVSEISKTSRNAMGVKLMRTSEEEKIVAIA
KIKSEDIIEEEILNEENLNEENLNEENLNEENLNEENLNEENLNEE

Sequences:

>Translated_846_residues
MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVHRRIIYSMQGLGLAPEKGYRKCARIVGDVLGKYHPHG
DTAVYEALVRMAQNFSIRYTLVDGHGNFGSVDGDGAAAMRYTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPS
RFPNLLVNGSSGIAVGMATNIPPHNLTEVIDGIIMLIENEDVNILDLMTKIKGPDFPTSGLIVGTRGIREAYETGRGKVI
IRAKAEIEEEKGKNKIIVTEIPYQVNKARLIENMANLVKDKKINGISDLRDESDRDGMRIVIELKRDANPNIVLNQLYKH
TKLQDTFGIIMLALVNNQPQILNLKEILVNYVEFQKEVIRRRTRFDLDKALARAHILEGLRIALDHIDEVIKLIRASKNT
AEAKEGLMNNFNLSEKQAQAILDMKLQRLTGLEREKIEEEYKELMEKISYFREILDKEELVLSIIKEELIEIKNKYGDER
KTEIVKGEHDIDIEDLIEDKKVIVTLTHGGYIKRLDMDTYSSQKRGGKGIQATSTKQDDFIENMFVTSTHSTILFFTNRG
KVYKLKAYEIPEAGRTAKGTNIVNLIPIENNEKIQTVIGLKDIDDMKHFVMCTRNGIIKKTEISKYSSIRKGGLNAINLR
EDDELIDVKMTKGNDEIIVVTQNGYCIRFNEEDVRPMGRVATGVKAITLRKTDKAVSMDVVIEDETLLSISENGFGKRTD
IEEYPIHRRGGKGVITYKITDKTGPIVGARFVKEDDELMLVNSGDVAIRINVSEISKTSRNAMGVKLMRTSEEEKIVAIA
KIKSEDIIEEEILNEENLNEENLNEENLNEENLNEENLNEENLNEE
>Mature_846_residues
MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVHRRIIYSMQGLGLAPEKGYRKCARIVGDVLGKYHPHG
DTAVYEALVRMAQNFSIRYTLVDGHGNFGSVDGDGAAAMRYTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPS
RFPNLLVNGSSGIAVGMATNIPPHNLTEVIDGIIMLIENEDVNILDLMTKIKGPDFPTSGLIVGTRGIREAYETGRGKVI
IRAKAEIEEEKGKNKIIVTEIPYQVNKARLIENMANLVKDKKINGISDLRDESDRDGMRIVIELKRDANPNIVLNQLYKH
TKLQDTFGIIMLALVNNQPQILNLKEILVNYVEFQKEVIRRRTRFDLDKALARAHILEGLRIALDHIDEVIKLIRASKNT
AEAKEGLMNNFNLSEKQAQAILDMKLQRLTGLEREKIEEEYKELMEKISYFREILDKEELVLSIIKEELIEIKNKYGDER
KTEIVKGEHDIDIEDLIEDKKVIVTLTHGGYIKRLDMDTYSSQKRGGKGIQATSTKQDDFIENMFVTSTHSTILFFTNRG
KVYKLKAYEIPEAGRTAKGTNIVNLIPIENNEKIQTVIGLKDIDDMKHFVMCTRNGIIKKTEISKYSSIRKGGLNAINLR
EDDELIDVKMTKGNDEIIVVTQNGYCIRFNEEDVRPMGRVATGVKAITLRKTDKAVSMDVVIEDETLLSISENGFGKRTD
IEEYPIHRRGGKGVITYKITDKTGPIVGARFVKEDDELMLVNSGDVAIRINVSEISKTSRNAMGVKLMRTSEEEKIVAIA
KIKSEDIIEEEILNEENLNEENLNEENLNEENLNEENLNEENLNEE

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=317, Percent_Identity=24.9211356466877, Blast_Score=84, Evalue=6e-16,
Organism=Homo sapiens, GI19913406, Length=177, Percent_Identity=28.2485875706215, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788562, Length=843, Percent_Identity=50.7710557532622, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=725, Percent_Identity=35.7241379310345, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17535065, Length=176, Percent_Identity=29.5454545454545, Blast_Score=74, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI212645845, Length=476, Percent_Identity=23.9495798319328, Blast_Score=73, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=378, Percent_Identity=24.8677248677249, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17136538, Length=363, Percent_Identity=23.1404958677686, Blast_Score=74, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 95660; Mature: 95660

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVHRRIIYSMQGLGLAPEK
CCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
GYRKCARIVGDVLGKYHPHGDTAVYEALVRMAQNFSIRYTLVDGHGNFGSVDGDGAAAMR
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCHHEE
YTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPSRFPNLLVNGSSGIAVGMATN
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHCCCCEEEECCCCEEEEEECC
IPPHNLTEVIDGIIMLIENEDVNILDLMTKIKGPDFPTSGLIVGTRGIREAYETGRGKVI
CCCCHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCCEEE
IRAKAEIEEEKGKNKIIVTEIPYQVNKARLIENMANLVKDKKINGISDLRDESDRDGMRI
EEEECHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEE
VIELKRDANPNIVLNQLYKHTKLQDTFGIIMLALVNNQPQILNLKEILVNYVEFQKEVIR
EEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCEECHHHHHHHHHHHHHHHHH
RRTRFDLDKALARAHILEGLRIALDHIDEVIKLIRASKNTAEAKEGLMNNFNLSEKQAQA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHH
ILDMKLQRLTGLEREKIEEEYKELMEKISYFREILDKEELVLSIIKEELIEIKNKYGDER
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCH
KTEIVKGEHDIDIEDLIEDKKVIVTLTHGGYIKRLDMDTYSSQKRGGKGIQATSTKQDDF
HHHHCCCCCCCCHHHHHCCCEEEEEEECCCEEEEEECCCCCCHHCCCCCCEECCCCCHHH
IENMFVTSTHSTILFFTNRGKVYKLKAYEIPEAGRTAKGTNIVNLIPIENNEKIQTVIGL
HHHHEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECC
KDIDDMKHFVMCTRNGIIKKTEISKYSSIRKGGLNAINLREDDELIDVKMTKGNDEIIVV
CCHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCEEEEE
TQNGYCIRFNEEDVRPMGRVATGVKAITLRKTDKAVSMDVVIEDETLLSISENGFGKRTD
ECCCEEEEECCHHCCHHHHHHCCCEEEEEEECCCCEEEEEEEECCEEEEECCCCCCCCCC
IEEYPIHRRGGKGVITYKITDKTGPIVGARFVKEDDELMLVNSGDVAIRINVSEISKTSR
CHHCCCCCCCCCEEEEEEEECCCCCEEEHHEECCCCCEEEEECCCEEEEEEHHHHHHHCC
NAMGVKLMRTSEEEKIVAIAKIKSEDIIEEEILNEENLNEENLNEENLNEENLNEENLNE
CCCCEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ENLNEE
CCCCCC
>Mature Secondary Structure
MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVHRRIIYSMQGLGLAPEK
CCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
GYRKCARIVGDVLGKYHPHGDTAVYEALVRMAQNFSIRYTLVDGHGNFGSVDGDGAAAMR
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCHHEE
YTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPSRFPNLLVNGSSGIAVGMATN
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHCCCCEEEECCCCEEEEEECC
IPPHNLTEVIDGIIMLIENEDVNILDLMTKIKGPDFPTSGLIVGTRGIREAYETGRGKVI
CCCCHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCCEEE
IRAKAEIEEEKGKNKIIVTEIPYQVNKARLIENMANLVKDKKINGISDLRDESDRDGMRI
EEEECHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEE
VIELKRDANPNIVLNQLYKHTKLQDTFGIIMLALVNNQPQILNLKEILVNYVEFQKEVIR
EEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCEECHHHHHHHHHHHHHHHHH
RRTRFDLDKALARAHILEGLRIALDHIDEVIKLIRASKNTAEAKEGLMNNFNLSEKQAQA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHH
ILDMKLQRLTGLEREKIEEEYKELMEKISYFREILDKEELVLSIIKEELIEIKNKYGDER
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCH
KTEIVKGEHDIDIEDLIEDKKVIVTLTHGGYIKRLDMDTYSSQKRGGKGIQATSTKQDDF
HHHHCCCCCCCCHHHHHCCCEEEEEEECCCEEEEEECCCCCCHHCCCCCCEECCCCCHHH
IENMFVTSTHSTILFFTNRGKVYKLKAYEIPEAGRTAKGTNIVNLIPIENNEKIQTVIGL
HHHHEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECC
KDIDDMKHFVMCTRNGIIKKTEISKYSSIRKGGLNAINLREDDELIDVKMTKGNDEIIVV
CCHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCEEEEE
TQNGYCIRFNEEDVRPMGRVATGVKAITLRKTDKAVSMDVVIEDETLLSISENGFGKRTD
ECCCEEEEECCHHCCHHHHHHCCCEEEEEEECCCCEEEEEEEECCEEEEECCCCCCCCCC
IEEYPIHRRGGKGVITYKITDKTGPIVGARFVKEDDELMLVNSGDVAIRINVSEISKTSR
CHHCCCCCCCCCEEEEEEEECCCCCEEEHHEECCCCCEEEEECCCEEEEEEHHHHHHHCC
NAMGVKLMRTSEEEKIVAIAKIKSEDIIEEEILNEENLNEENLNEENLNEENLNEENLNE
CCCCEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ENLNEE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]