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The map label for this gene is gyrB [H]

Identifier: 226947228

GI number: 226947228

Start: 4560

End: 6473

Strand: Direct

Name: gyrB [H]

Synonym: CLM_0006

Alternate gene names: 226947228

Gene position: 4560-6473 (Clockwise)

Preceding gene: 226947227

Following gene: 226947229

Centisome position: 0.11

GC content: 31.87

Gene sequence:

>1914_bases
ATGTCACAAGAAAATAAACAAGTTTATGATGAAAGTCAAATTCAAGTGTTGGAAGGTTTAGAAGCTGTTAGAAAAAGACC
AGGAATGTATATTGGAAGCACTAGTTTAAGGGGACTTCATCATTTGGTTTATGAAATAGTTGATAATAGTATAGATGAAG
CGCTAGCTGGTTTTTGTACACATATAGAAGTTTTTATACACAAAGATAATTCTATAACTGTGGTAGACGATGGACGTGGA
ATGCCAGTTGGTATGCATTCAAAGGTAAAAAAACCTGCTGTTGAAGTAATAATGACTATATTACATGCAGGTGGAAAATT
TGGTGGTGGAGGCTACAAGGTTTCTGGTGGACTTCATGGTGTTGGAGCTTCTGTTGTTAATGCCTTATCAGAACAATGTG
AAGTAGAAGTAAGAAGAGAAGGCCATATTTGGAAACAAAAATTCGAAAGAGGTATAACAAAAACTGGATTAGATATAGTT
GGAGATACGGAAGATCATGGTACAAAAATATATTTTAAGCCAGATATTGAAATATTTGATGAATTAGAATTTGAATATGA
TACTTTAGCTCACAGACTCAGAGAATTAGCCTTTTTAAACAAAGGTATAAAAATTTCATTAACAGATGAAAGATATGATA
AAAAAGAAATGTTCCACTATGAGGGCGGATTGAGATCTTTTGTACTTTATTTAAATAGAAATAAGGAAAAATTACATCAA
CAACCTATATATGTTGATGAAAATAAAGATGGATGTATAGTTGAGATTGCTATGCAATATAACGATGGTTATGCAGAAAA
TATTTTTTCCTTTGCTAACAATATAGATACCATAGAAGGTGGAACTCATTTAGCTGGATTTAAATCAGCTCTAACAAGAG
TTATAAATGACTATGCTAGAAAATTTAATTACTTAAAGGATATAGATAAAAATTTATCAGGAGACGATGTTAGGGAAGGT
CTAACAGCAGTTATTTCAGTAAAATTAACAGATCCTCAATTTGAAGGACAGACAAAGACAAAATTAGGAAATGGTGAAGT
AAGAGGAATTGTAGATACAATAGTAGGTCAGAGTATAGGTTCTTTTTTAGAGGAAAATCCTAATGTAGGTAAAATAATAA
TTGAAAAAGGCCTATCAGCTTCAAGAGCTAGAGAGGCTGCAAAAAGAGCAAGAGAACTTACTAGAAGAAAATCTATTTTA
GAAAGCACGTCTCTTCCAGGAAAATTATCAGATTGTTCTTCAAAGGATCCATCATTATGTGAAATTTATTTGGTCGAAGG
AGATTCCGCAGGTGGATCTGCAAAACAAGGAAGAAATAGAGAATTCCAAGCTATATTACCTTTAAAGGGTAAAATAATGA
ATGTGGAAAAGCAAAGATTGGATAAAATATTAGCTTCAGATGAAATACGTGCTATGATAACTGCATTTGGAGCAGGTATA
GGAAAAGAATTTGATATTGATAAAATAAGATATAATAGAATAATTATAATGACAGATGCTGACGTAGATGGAGCCCATAT
AAGAACTTTACTTTTAACATTCTTTTATAGATACATGAAAGAATTAATAGAGGAAGGTCATGTTTATATAGCTCAACCAC
CACTTTATAGAATATATAAGGCTAAAAAAGAAATTTATGTTTATTCAGATCCAGAATTGGATGCTGCATTATTAGAATTG
GGTGGAAAGGATACCAATACTAATATTCAAAGATACAAAGGTCTTGGAGAAATGAATCCAGAACAACTTTGGGAAACTAC
TATGGACCCAGAACATAGAACTTTATTACAGGTAACAGTAGAGGACGCTATGGAGGCAGATGAAATATTTACTATACTTA
TGGGAACAAAAGTAGAACCAAGAAGAGACTTTATAGAAAGTAATGCTGATAAAGTAGTTAACTTGGATATATAA

Upstream 100 bases:

>100_bases
ATAAAAAGAGTAAGATATATTTGTCTCCTATATCTTCAGCTACACTGACTAAAAGAACAAAAGTTTTGTATAATGAGCTG
TAAAAGTTAGGAGGATAAAC

Downstream 100 bases:

>100_bases
GATAAGATTGTAAAGAGGTGTAATCATGCTAAACGAAGGAAAGATTTTGCCTGTAGATGTAAGTAAAGAAATGAAGAAAT
GTTATATAGACTATGCCATG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MSQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVYEIVDNSIDEALAGFCTHIEVFIHKDNSITVVDDGRG
MPVGMHSKVKKPAVEVIMTILHAGGKFGGGGYKVSGGLHGVGASVVNALSEQCEVEVRREGHIWKQKFERGITKTGLDIV
GDTEDHGTKIYFKPDIEIFDELEFEYDTLAHRLRELAFLNKGIKISLTDERYDKKEMFHYEGGLRSFVLYLNRNKEKLHQ
QPIYVDENKDGCIVEIAMQYNDGYAENIFSFANNIDTIEGGTHLAGFKSALTRVINDYARKFNYLKDIDKNLSGDDVREG
LTAVISVKLTDPQFEGQTKTKLGNGEVRGIVDTIVGQSIGSFLEENPNVGKIIIEKGLSASRAREAAKRARELTRRKSIL
ESTSLPGKLSDCSSKDPSLCEIYLVEGDSAGGSAKQGRNREFQAILPLKGKIMNVEKQRLDKILASDEIRAMITAFGAGI
GKEFDIDKIRYNRIIIMTDADVDGAHIRTLLLTFFYRYMKELIEEGHVYIAQPPLYRIYKAKKEIYVYSDPELDAALLEL
GGKDTNTNIQRYKGLGEMNPEQLWETTMDPEHRTLLQVTVEDAMEADEIFTILMGTKVEPRRDFIESNADKVVNLDI

Sequences:

>Translated_637_residues
MSQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVYEIVDNSIDEALAGFCTHIEVFIHKDNSITVVDDGRG
MPVGMHSKVKKPAVEVIMTILHAGGKFGGGGYKVSGGLHGVGASVVNALSEQCEVEVRREGHIWKQKFERGITKTGLDIV
GDTEDHGTKIYFKPDIEIFDELEFEYDTLAHRLRELAFLNKGIKISLTDERYDKKEMFHYEGGLRSFVLYLNRNKEKLHQ
QPIYVDENKDGCIVEIAMQYNDGYAENIFSFANNIDTIEGGTHLAGFKSALTRVINDYARKFNYLKDIDKNLSGDDVREG
LTAVISVKLTDPQFEGQTKTKLGNGEVRGIVDTIVGQSIGSFLEENPNVGKIIIEKGLSASRAREAAKRARELTRRKSIL
ESTSLPGKLSDCSSKDPSLCEIYLVEGDSAGGSAKQGRNREFQAILPLKGKIMNVEKQRLDKILASDEIRAMITAFGAGI
GKEFDIDKIRYNRIIIMTDADVDGAHIRTLLLTFFYRYMKELIEEGHVYIAQPPLYRIYKAKKEIYVYSDPELDAALLEL
GGKDTNTNIQRYKGLGEMNPEQLWETTMDPEHRTLLQVTVEDAMEADEIFTILMGTKVEPRRDFIESNADKVVNLDI
>Mature_636_residues
SQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVYEIVDNSIDEALAGFCTHIEVFIHKDNSITVVDDGRGM
PVGMHSKVKKPAVEVIMTILHAGGKFGGGGYKVSGGLHGVGASVVNALSEQCEVEVRREGHIWKQKFERGITKTGLDIVG
DTEDHGTKIYFKPDIEIFDELEFEYDTLAHRLRELAFLNKGIKISLTDERYDKKEMFHYEGGLRSFVLYLNRNKEKLHQQ
PIYVDENKDGCIVEIAMQYNDGYAENIFSFANNIDTIEGGTHLAGFKSALTRVINDYARKFNYLKDIDKNLSGDDVREGL
TAVISVKLTDPQFEGQTKTKLGNGEVRGIVDTIVGQSIGSFLEENPNVGKIIIEKGLSASRAREAAKRARELTRRKSILE
STSLPGKLSDCSSKDPSLCEIYLVEGDSAGGSAKQGRNREFQAILPLKGKIMNVEKQRLDKILASDEIRAMITAFGAGIG
KEFDIDKIRYNRIIIMTDADVDGAHIRTLLLTFFYRYMKELIEEGHVYIAQPPLYRIYKAKKEIYVYSDPELDAALLELG
GKDTNTNIQRYKGLGEMNPEQLWETTMDPEHRTLLQVTVEDAMEADEIFTILMGTKVEPRRDFIESNADKVVNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=668, Percent_Identity=26.7964071856287, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI19913406, Length=619, Percent_Identity=25.686591276252, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI48994957, Length=565, Percent_Identity=57.3451327433628, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=40.4761904761905, Blast_Score=466, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17535065, Length=669, Percent_Identity=25.7100149476831, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI212645845, Length=613, Percent_Identity=25.9380097879282, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=270, Percent_Identity=26.2962962962963, Blast_Score=86, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324241, Length=626, Percent_Identity=26.1980830670927, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17136538, Length=681, Percent_Identity=24.2290748898678, Blast_Score=146, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71571; Mature: 71440

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVYEIVDNSIDEALAGFCT
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHH
HIEVFIHKDNSITVVDDGRGMPVGMHSKVKKPAVEVIMTILHAGGKFGGGGYKVSGGLHG
EEEEEEECCCEEEEEECCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGASVVNALSEQCEVEVRREGHIWKQKFERGITKTGLDIVGDTEDHGTKIYFKPDIEIFD
HHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCEEECCCCCCCCEEEECCCCHHHH
ELEFEYDTLAHRLRELAFLNKGIKISLTDERYDKKEMFHYEGGLRSFVLYLNRNKEKLHQ
HCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECCHHHHHC
QPIYVDENKDGCIVEIAMQYNDGYAENIFSFANNIDTIEGGTHLAGFKSALTRVINDYAR
CCEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KFNYLKDIDKNLSGDDVREGLTAVISVKLTDPQFEGQTKTKLGNGEVRGIVDTIVGQSIG
HHHHHHHHHCCCCCHHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SFLEENPNVGKIIIEKGLSASRAREAAKRARELTRRKSILESTSLPGKLSDCSSKDPSLC
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
EIYLVEGDSAGGSAKQGRNREFQAILPLKGKIMNVEKQRLDKILASDEIRAMITAFGAGI
EEEEEECCCCCCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCC
GKEFDIDKIRYNRIIIMTDADVDGAHIRTLLLTFFYRYMKELIEEGHVYIAQPPLYRIYK
CCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
AKKEIYVYSDPELDAALLELGGKDTNTNIQRYKGLGEMNPEQLWETTMDPEHRTLLQVTV
CCCEEEEEECCCHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEH
EDAMEADEIFTILMGTKVEPRRDFIESNADKVVNLDI
HHHHCHHHHHHHHHCCCCCHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
SQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVYEIVDNSIDEALAGFCT
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHH
HIEVFIHKDNSITVVDDGRGMPVGMHSKVKKPAVEVIMTILHAGGKFGGGGYKVSGGLHG
EEEEEEECCCEEEEEECCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGASVVNALSEQCEVEVRREGHIWKQKFERGITKTGLDIVGDTEDHGTKIYFKPDIEIFD
HHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCEEECCCCCCCCEEEECCCCHHHH
ELEFEYDTLAHRLRELAFLNKGIKISLTDERYDKKEMFHYEGGLRSFVLYLNRNKEKLHQ
HCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECCHHHHHC
QPIYVDENKDGCIVEIAMQYNDGYAENIFSFANNIDTIEGGTHLAGFKSALTRVINDYAR
CCEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KFNYLKDIDKNLSGDDVREGLTAVISVKLTDPQFEGQTKTKLGNGEVRGIVDTIVGQSIG
HHHHHHHHHCCCCCHHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SFLEENPNVGKIIIEKGLSASRAREAAKRARELTRRKSILESTSLPGKLSDCSSKDPSLC
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
EIYLVEGDSAGGSAKQGRNREFQAILPLKGKIMNVEKQRLDKILASDEIRAMITAFGAGI
EEEEEECCCCCCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCC
GKEFDIDKIRYNRIIIMTDADVDGAHIRTLLLTFFYRYMKELIEEGHVYIAQPPLYRIYK
CCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
AKKEIYVYSDPELDAALLELGGKDTNTNIQRYKGLGEMNPEQLWETTMDPEHRTLLQVTV
CCCEEEEEECCCHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEH
EDAMEADEIFTILMGTKVEPRRDFIESNADKVVNLDI
HHHHCHHHHHHHHHCCCCCHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]