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The map label for this gene is truA

Identifier: 226225144

GI number: 226225144

Start: 2651802

End: 2652548

Strand: Reverse

Name: truA

Synonym: Lm4b_02565

Alternate gene names: 226225144

Gene position: 2652548-2651802 (Counterclockwise)

Preceding gene: 226225145

Following gene: 226225143

Centisome position: 91.07

GC content: 40.56

Gene sequence:

>747_bases
ATGACGAGATATAAAGCGATAATTTCTTATGATGGTAGCGGGTTTTATGGCTATCAGGTGCAGCCGAATACACGGACGGT
CCAAGCGGAAATTGAAAAAGCACTGACAAAAATGCATAAAGGCAAGACTGTGCGGATTACAGCATCAGGAAGAACAGATA
CTGGTGTTCATGCGAAAGGTCAGGTTATTCATTTCGATTCGGAACTTGATATCACTGCCGAAAAATTTCAAAAAGCTTTG
CAAGTCATGACTCCGTTCGATATTAGCTTTTTAACAGTGGAAGAAGTACCAGACGATTTCCACGCTAGATTTGGCACAGT
TGGAAAAGAATATCGTTATGTGGTAAAGCGGACGAAAATTTTTGATCCTTTTAGTCGGAATTTTGCGCTACATTATCCAT
ATGAACTGGACATTTCAAAAATGAAATTGGCAAGTAAACGCCTGATTGGCGAGCATGATTTTACAAGTTTCTGTTCGGCG
AGAACGGAGCGCGATTCTAAAGTCCGAACACTTTATAGTATTGATTTTTATGAAGAGGACGACGAGACGTTAGTGATTGC
TTTTCAAGGGAATGGCTTTTTATATAATATGGTGCGAATTTTGACGGGAACTTTGCTCGATGCAGGGCAAGGTCGAATCT
CTCCGGATGATATTAGTGAGGCTTTATTAGCACGTGACCGGCAAAAATTAATTAGTAAAACTGCGCCGCCACAAGGGTTA
TATTTATGGCGAGTGGACTATGAATAA

Upstream 100 bases:

>100_bases
CGAGATTCCGTTTACTACGCTGGCGATTTGCAGATACGTTGCTACTGATTTCGTTAGTAGTGTTAAGTGGATTATTATTT
TGGTTGCGGAGTTGAAATAA

Downstream 100 bases:

>100_bases
AAATTGGATGTTTTTTTGCAGAACGCATTGACTTTTTGCTCCAAAAACTGTATTATGGACTATGGTATTGTTTGCCCCAC
AATAAGCCCCGGAAGGTTGT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTRYKAIISYDGSGFYGYQVQPNTRTVQAEIEKALTKMHKGKTVRITASGRTDTGVHAKGQVIHFDSELDITAEKFQKAL
QVMTPFDISFLTVEEVPDDFHARFGTVGKEYRYVVKRTKIFDPFSRNFALHYPYELDISKMKLASKRLIGEHDFTSFCSA
RTERDSKVRTLYSIDFYEEDDETLVIAFQGNGFLYNMVRILTGTLLDAGQGRISPDDISEALLARDRQKLISKTAPPQGL
YLWRVDYE

Sequences:

>Translated_248_residues
MTRYKAIISYDGSGFYGYQVQPNTRTVQAEIEKALTKMHKGKTVRITASGRTDTGVHAKGQVIHFDSELDITAEKFQKAL
QVMTPFDISFLTVEEVPDDFHARFGTVGKEYRYVVKRTKIFDPFSRNFALHYPYELDISKMKLASKRLIGEHDFTSFCSA
RTERDSKVRTLYSIDFYEEDDETLVIAFQGNGFLYNMVRILTGTLLDAGQGRISPDDISEALLARDRQKLISKTAPPQGL
YLWRVDYE
>Mature_247_residues
TRYKAIISYDGSGFYGYQVQPNTRTVQAEIEKALTKMHKGKTVRITASGRTDTGVHAKGQVIHFDSELDITAEKFQKALQ
VMTPFDISFLTVEEVPDDFHARFGTVGKEYRYVVKRTKIFDPFSRNFALHYPYELDISKMKLASKRLIGEHDFTSFCSAR
TERDSKVRTLYSIDFYEEDDETLVIAFQGNGFLYNMVRILTGTLLDAGQGRISPDDISEALLARDRQKLISKTAPPQGLY
LWRVDYE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=30.0724637681159, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI199559785, Length=259, Percent_Identity=27.4131274131274, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788657, Length=240, Percent_Identity=35, Blast_Score=154, Evalue=4e-39,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=27.1523178807947, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28414; Mature: 28282

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYKAIISYDGSGFYGYQVQPNTRTVQAEIEKALTKMHKGKTVRITASGRTDTGVHAKG
CCCEEEEEEECCCCEEEEEECCCCEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
QVIHFDSELDITAEKFQKALQVMTPFDISFLTVEEVPDDFHARFGTVGKEYRYVVKRTKI
EEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEHHHCCCHHHHHHCCCCHHHHHHHHHHHC
FDPFSRNFALHYPYELDISKMKLASKRLIGEHDFTSFCSARTERDSKVRTLYSIDFYEED
CCCCCCCEEEECCEECCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEEEEEEECC
DETLVIAFQGNGFLYNMVRILTGTLLDAGQGRISPDDISEALLARDRQKLISKTAPPQGL
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
YLWRVDYE
EEEEEECC
>Mature Secondary Structure 
TRYKAIISYDGSGFYGYQVQPNTRTVQAEIEKALTKMHKGKTVRITASGRTDTGVHAKG
CCEEEEEEECCCCEEEEEECCCCEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
QVIHFDSELDITAEKFQKALQVMTPFDISFLTVEEVPDDFHARFGTVGKEYRYVVKRTKI
EEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEHHHCCCHHHHHHCCCCHHHHHHHHHHHC
FDPFSRNFALHYPYELDISKMKLASKRLIGEHDFTSFCSARTERDSKVRTLYSIDFYEED
CCCCCCCEEEECCEECCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEEEEEEECC
DETLVIAFQGNGFLYNMVRILTGTLLDAGQGRISPDDISEALLARDRQKLISKTAPPQGL
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
YLWRVDYE
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]