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The map label for this gene is prfB

Identifier: 226225057

GI number: 226225057

Start: 2558855

End: 2559871

Strand: Reverse

Name: prfB

Synonym: Lm4b_02478

Alternate gene names: 226225057

Gene position: 2559871-2558855 (Counterclockwise)

Preceding gene: 226225058

Following gene: 226225056

Centisome position: 87.89

GC content: 38.35

Gene sequence:

>1017_bases
ATGGAAGTTCGAATCGCTGAGCTAGAAGACCAAATGTTAGATCCGAATTTTTGGAATGACCAACAAGCGGCACAGAAAGT
AATTAATGAGTCAAACGGATATAAAGAAACATACCAAGCTTTTCATGCGCTAGAAGAAGAACAAGAAAGTATGGAAATTA
GTCTAGAATTATTAAAAGAAGAAGCAGATGAAGATTTACAAGAAGAATTAGAAAAAGATATTAAAGCTTACATGGCGACT
ATTAGTGAGTTTGAATTAAAACTGATGCTAAGTGATCCATATGACAAAAATAATGCTATTTTAGAACTTCATCCAGGTGC
TGGTGGTACAGAATCACAAGACTGGGGTTCGATGTTACTTCGTATGTATCAACGTTGGTCTGAGAAGAAAGGCTTTAAAG
TAGAGATGCTTGATTACCAAGCAGGGGACGAAGCTGGAATAAAAAGTGTCACGTTACTTATAAAAGGCCACAATGCTTAC
GGCTATTTAAAAGCGGAAAAAGGGGTACATCGTCTAGTGCGTATTTCGCCATTTGACTCATCCGGCAGACGTCATACATC
ATTCGTTTCCGTGGATGTAATGCCTGAATTAGATGATGATATTGAAATTGAAGTACGTACAGAAGACTTGAAAATTGATA
CGTACCGCGCAACTGGTGCAGGTGGACAACATATCAATACAACTGACTCAGCTGTTAGGATGACACATATTCCATCCGGC
ATCGTTGTAACGTGCCAATCTGAGCGTTCTCAGCTTAAAAACCGTGAACAGGCAATGAAAATGTTAAAAACAAAATTATA
CCAAAAAGAACAAGAGGAAAAAGAACGCGAGCTAGCAGAAATTCGAGGAGAACAAAAAGAAATTGGTTGGGGAAGCCAAA
TTCGTTCCTATGTTTTCCACCCTTACTCCATGGTGAAAGATCATCGCACAAATTACGAAACCGGTAATATTCAAGCGGTA
ATGGATGGAGACTTAGATGAATTTATCAATGCATATTTGCGTTCTAGGATTGGATAA

Upstream 100 bases:

>100_bases
AAAGGTGGAATTAGAATGGAATTAGCAGAAATTCGTAATGAATTAGAAAAAACAGCGCAGCAAATCAAAGACTTTAGGGG
GTCTCTTTGACTTAGACAGT

Downstream 100 bases:

>100_bases
AAGAGATGGCAAATAAAAGAAAGAAACAACCGTTATCCCCAGTTGTTTCTAAATTAATTGAGTATATTTATGTCATTATA
GGTGCAGCTCTCATCGCACT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MEVRIAELEDQMLDPNFWNDQQAAQKVINESNGYKETYQAFHALEEEQESMEISLELLKEEADEDLQEELEKDIKAYMAT
ISEFELKLMLSDPYDKNNAILELHPGAGGTESQDWGSMLLRMYQRWSEKKGFKVEMLDYQAGDEAGIKSVTLLIKGHNAY
GYLKAEKGVHRLVRISPFDSSGRRHTSFVSVDVMPELDDDIEIEVRTEDLKIDTYRATGAGGQHINTTDSAVRMTHIPSG
IVVTCQSERSQLKNREQAMKMLKTKLYQKEQEEKERELAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTNYETGNIQAV
MDGDLDEFINAYLRSRIG

Sequences:

>Translated_338_residues
MEVRIAELEDQMLDPNFWNDQQAAQKVINESNGYKETYQAFHALEEEQESMEISLELLKEEADEDLQEELEKDIKAYMAT
ISEFELKLMLSDPYDKNNAILELHPGAGGTESQDWGSMLLRMYQRWSEKKGFKVEMLDYQAGDEAGIKSVTLLIKGHNAY
GYLKAEKGVHRLVRISPFDSSGRRHTSFVSVDVMPELDDDIEIEVRTEDLKIDTYRATGAGGQHINTTDSAVRMTHIPSG
IVVTCQSERSQLKNREQAMKMLKTKLYQKEQEEKERELAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTNYETGNIQAV
MDGDLDEFINAYLRSRIG
>Mature_338_residues
MEVRIAELEDQMLDPNFWNDQQAAQKVINESNGYKETYQAFHALEEEQESMEISLELLKEEADEDLQEELEKDIKAYMAT
ISEFELKLMLSDPYDKNNAILELHPGAGGTESQDWGSMLLRMYQRWSEKKGFKVEMLDYQAGDEAGIKSVTLLIKGHNAY
GYLKAEKGVHRLVRISPFDSSGRRHTSFVSVDVMPELDDDIEIEVRTEDLKIDTYRATGAGGQHINTTDSAVRMTHIPSG
IVVTCQSERSQLKNREQAMKMLKTKLYQKEQEEKERELAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTNYETGNIQAV
MDGDLDEFINAYLRSRIG

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=295, Percent_Identity=37.9661016949153, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI34577120, Length=312, Percent_Identity=38.7820512820513, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI166795305, Length=200, Percent_Identity=36.5, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI2367172, Length=332, Percent_Identity=48.4939759036145, Blast_Score=324, Evalue=5e-90,
Organism=Escherichia coli, GI1787462, Length=335, Percent_Identity=35.8208955223881, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=305, Percent_Identity=31.4754098360656, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=308, Percent_Identity=35.7142857142857, Blast_Score=160, Evalue=3e-40,
Organism=Drosophila melanogaster, GI19921226, Length=273, Percent_Identity=37.3626373626374, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 38894; Mature: 38894

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVRIAELEDQMLDPNFWNDQQAAQKVINESNGYKETYQAFHALEEEQESMEISLELLKE
CCEEEECHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EADEDLQEELEKDIKAYMATISEFELKLMLSDPYDKNNAILELHPGAGGTESQDWGSMLL
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHH
RMYQRWSEKKGFKVEMLDYQAGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDS
HHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEHHHCCEEEEEECCCCC
SGRRHTSFVSVDVMPELDDDIEIEVRTEDLKIDTYRATGAGGQHINTTDSAVRMTHIPSG
CCCCEEEEEEEEECCCCCCCEEEEEEECCEEEEEEEECCCCCCCCCCCCHHEEEEECCCC
IVVTCQSERSQLKNREQAMKMLKTKLYQKEQEEKERELAEIRGEQKEIGWGSQIRSYVFH
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCC
PYSMVKDHRTNYETGNIQAVMDGDLDEFINAYLRSRIG
CHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEVRIAELEDQMLDPNFWNDQQAAQKVINESNGYKETYQAFHALEEEQESMEISLELLKE
CCEEEECHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EADEDLQEELEKDIKAYMATISEFELKLMLSDPYDKNNAILELHPGAGGTESQDWGSMLL
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHH
RMYQRWSEKKGFKVEMLDYQAGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDS
HHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEHHHCCEEEEEECCCCC
SGRRHTSFVSVDVMPELDDDIEIEVRTEDLKIDTYRATGAGGQHINTTDSAVRMTHIPSG
CCCCEEEEEEEEECCCCCCCEEEEEEECCEEEEEEEECCCCCCCCCCCCHHEEEEECCCC
IVVTCQSERSQLKNREQAMKMLKTKLYQKEQEEKERELAEIRGEQKEIGWGSQIRSYVFH
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCC
PYSMVKDHRTNYETGNIQAVMDGDLDEFINAYLRSRIG
CHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]