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The map label for this gene is pgk

Identifier: 226225006

GI number: 226225006

Start: 2502716

End: 2503906

Strand: Reverse

Name: pgk

Synonym: Lm4b_02427

Alternate gene names: 226225006

Gene position: 2503906-2502716 (Counterclockwise)

Preceding gene: 226225007

Following gene: 226225005

Centisome position: 85.97

GC content: 39.55

Gene sequence:

>1191_bases
ATGGCTAAAAAAGTTGTAACTGATTTAGATTTAAAAGACAAAAAAGTTTTAGTTCGTGTTGACTTTAACGTGCCAATGAA
AGACGGCAAAATCACTAACGACAACCGTATTGTTGCTGCACTTCCAACAATTGAGTACATCTTAGAACAAAACGGTAAAG
CAATTCTATTTTCTCACCTTGGAAAAGTAAAAACAGAAGAAGATAAAGAAGGAAAATCTCTTCGTCCAGTAGCCGCTCGT
TTAAGCGAATTACTTGGAAAAGAAGTGAAATTCGTTCCAACTACTCGTGGTCCAGAACTTGAGAAAGCAATTGATGAGTT
AAAAGACGGCGAAGTACTTCTTTTTGAAAATACACGTTTTGAAGATATTGATGGTAAAAAAGAAAGCAAAAATGATCCAG
AACTTGGAAAATACTGGGCTAGCCTTGGCGACGTTTTCGTAAATGACGCTTTCGGTACTGCTCACCGTGCGCACGCGTCT
AACGTTGGAATCGCTTCTAACTTAGAATCAGCGGCCGGATTTTTGATGGAAAAAGAAATTAAATTCATCGGCGGTGTAGT
TGATAATCCAGCACGTCCACTAGTAGCAATCCTAGGTGGCGCAAAAGTTTCTGACAAAATCGGTGTTATCGAAAACTTAC
TTACAAAAGCAGACAAAGTTCTTGTCGGCGGCGGAATGACTTTCACTTTCATGGCAGCTCAAGGTCAAGAAATTGGTAAA
TCTCTTTTAGAAGCAGATAAAGTTGAACTTGCTAAAGGCTTACTTGAAAAAGCTGGCAATAAATTAGTATTGCCAGTTGA
TGCAGTTGTATCTAAAGAATTCAGTAACGATGCTCCTTTCCATACAGTGAGTGCAGATAGCATTCCAGCTGATGAAATGG
GACTAGATATTGGTCAAGCTACAATTGACTTATTTACAAAAGAACTTCAAGGCGCTAAAACAGTTGTATGGAATGGTCCA
ATGGGCGTATTCGAACTTAGCAACTTTGCTAAAGGTACAATTGGCGTTTGTGAAGCTATTGCAAACTTAACGGATGCAAC
AACAATCATCGGTGGTGGGGATTCCGCAGCAGCAGCTATGGACTTAGGTTTTGCTGACAAATTCACACATATTTCCACTG
GTGGCGGTGCATCATTAGAATATCTTGAAGGTAAAGAGCTTCCTGGCGTTGCTTCTATTAGCGACAAATAA

Upstream 100 bases:

>100_bases
TCAGCAATAACAAGAATAAGCGGAGACACTATTGTTTCCGCTTATTTTCTGAAATAACGGGGGCGACCCCAATCCTTTAA
AATTAATGGAGGAACTCTAA

Downstream 100 bases:

>100_bases
GCTGAACCAAAAATAATTTTTAATATCGAGAGGATGTTTATCCAAATGCGTAAACCAATTATTGCTGGTAACTGGAAAAT
GAACAAAACTGCTGCAAAAG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKKVVTDLDLKDKKVLVRVDFNVPMKDGKITNDNRIVAALPTIEYILEQNGKAILFSHLGKVKTEEDKEGKSLRPVAAR
LSELLGKEVKFVPTTRGPELEKAIDELKDGEVLLFENTRFEDIDGKKESKNDPELGKYWASLGDVFVNDAFGTAHRAHAS
NVGIASNLESAAGFLMEKEIKFIGGVVDNPARPLVAILGGAKVSDKIGVIENLLTKADKVLVGGGMTFTFMAAQGQEIGK
SLLEADKVELAKGLLEKAGNKLVLPVDAVVSKEFSNDAPFHTVSADSIPADEMGLDIGQATIDLFTKELQGAKTVVWNGP
MGVFELSNFAKGTIGVCEAIANLTDATTIIGGGDSAAAAMDLGFADKFTHISTGGGASLEYLEGKELPGVASISDK

Sequences:

>Translated_396_residues
MAKKVVTDLDLKDKKVLVRVDFNVPMKDGKITNDNRIVAALPTIEYILEQNGKAILFSHLGKVKTEEDKEGKSLRPVAAR
LSELLGKEVKFVPTTRGPELEKAIDELKDGEVLLFENTRFEDIDGKKESKNDPELGKYWASLGDVFVNDAFGTAHRAHAS
NVGIASNLESAAGFLMEKEIKFIGGVVDNPARPLVAILGGAKVSDKIGVIENLLTKADKVLVGGGMTFTFMAAQGQEIGK
SLLEADKVELAKGLLEKAGNKLVLPVDAVVSKEFSNDAPFHTVSADSIPADEMGLDIGQATIDLFTKELQGAKTVVWNGP
MGVFELSNFAKGTIGVCEAIANLTDATTIIGGGDSAAAAMDLGFADKFTHISTGGGASLEYLEGKELPGVASISDK
>Mature_395_residues
AKKVVTDLDLKDKKVLVRVDFNVPMKDGKITNDNRIVAALPTIEYILEQNGKAILFSHLGKVKTEEDKEGKSLRPVAARL
SELLGKEVKFVPTTRGPELEKAIDELKDGEVLLFENTRFEDIDGKKESKNDPELGKYWASLGDVFVNDAFGTAHRAHASN
VGIASNLESAAGFLMEKEIKFIGGVVDNPARPLVAILGGAKVSDKIGVIENLLTKADKVLVGGGMTFTFMAAQGQEIGKS
LLEADKVELAKGLLEKAGNKLVLPVDAVVSKEFSNDAPFHTVSADSIPADEMGLDIGQATIDLFTKELQGAKTVVWNGPM
GVFELSNFAKGTIGVCEAIANLTDATTIIGGGDSAAAAMDLGFADKFTHISTGGGASLEYLEGKELPGVASISDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=416, Percent_Identity=44.9519230769231, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI31543397, Length=416, Percent_Identity=43.9903846153846, Blast_Score=312, Evalue=3e-85,
Organism=Escherichia coli, GI1789294, Length=392, Percent_Identity=47.4489795918367, Blast_Score=315, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=46.3768115942029, Blast_Score=338, Evalue=4e-93,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=48.910411622276, Blast_Score=354, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24581203, Length=410, Percent_Identity=45.8536585365854, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI17136394, Length=409, Percent_Identity=44.7432762836186, Blast_Score=306, Evalue=1e-83,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42076; Mature: 41944

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKVVTDLDLKDKKVLVRVDFNVPMKDGKITNDNRIVAALPTIEYILEQNGKAILFSHL
CCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCEEEEECCHHHHHHHCCCCEEEEECC
GKVKTEEDKEGKSLRPVAARLSELLGKEVKFVPTTRGPELEKAIDELKDGEVLLFENTRF
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCC
EDIDGKKESKNDPELGKYWASLGDVFVNDAFGTAHRAHASNVGIASNLESAAGFLMEKEI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHCHHHHHHHHHHHHH
KFIGGVVDNPARPLVAILGGAKVSDKIGVIENLLTKADKVLVGGGMTFTFMAAQGQEIGK
HHHCCCCCCCCCCHHHEECCCCCCHHHHHHHHHHHHHCEEEECCCCEEEEEECCCHHHHH
SLLEADKVELAKGLLEKAGNKLVLPVDAVVSKEFSNDAPFHTVSADSIPADEMGLDIGQA
HHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCCCEEECCCCCCHHHHCCCHHHH
TIDLFTKELQGAKTVVWNGPMGVFELSNFAKGTIGVCEAIANLTDATTIIGGGDSAAAAM
HHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCHHHHH
DLGFADKFTHISTGGGASLEYLEGKELPGVASISDK
HCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKVVTDLDLKDKKVLVRVDFNVPMKDGKITNDNRIVAALPTIEYILEQNGKAILFSHL
CCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCEEEEECCHHHHHHHCCCCEEEEECC
GKVKTEEDKEGKSLRPVAARLSELLGKEVKFVPTTRGPELEKAIDELKDGEVLLFENTRF
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCC
EDIDGKKESKNDPELGKYWASLGDVFVNDAFGTAHRAHASNVGIASNLESAAGFLMEKEI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHCHHHHHHHHHHHHH
KFIGGVVDNPARPLVAILGGAKVSDKIGVIENLLTKADKVLVGGGMTFTFMAAQGQEIGK
HHHCCCCCCCCCCHHHEECCCCCCHHHHHHHHHHHHHCEEEECCCCEEEEEECCCHHHHH
SLLEADKVELAKGLLEKAGNKLVLPVDAVVSKEFSNDAPFHTVSADSIPADEMGLDIGQA
HHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCCCEEECCCCCCHHHHCCCHHHH
TIDLFTKELQGAKTVVWNGPMGVFELSNFAKGTIGVCEAIANLTDATTIIGGGDSAAAAM
HHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCHHHHH
DLGFADKFTHISTGGGASLEYLEGKELPGVASISDK
HCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]