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The map label for this gene is tpiA

Identifier: 226225005

GI number: 226225005

Start: 2501915

End: 2502670

Strand: Reverse

Name: tpiA

Synonym: Lm4b_02426

Alternate gene names: 226225005

Gene position: 2502670-2501915 (Counterclockwise)

Preceding gene: 226225006

Following gene: 226225004

Centisome position: 85.92

GC content: 41.93

Gene sequence:

>756_bases
ATGCGTAAACCAATTATTGCTGGTAACTGGAAAATGAACAAAACTGCTGCAAAAGCAGGACAATTTGCAGAAGACGTAAA
AAATAACGTGCCATCAAGCGATGCTGTTGAATCAGTAGTTGCTGCACCGGCGCTATTTTTACAAGAATTAGTTCGTCTTA
CTGAAGGAACTAATCTTCGCGTAGCTGCACAAAACTGTTATTTTGAAGACGAAGGTGCTTTCACTGGCGAAATTAGCCCG
TTTGCACTTGCTGATTTAGGTGTTTCTTATGTTATTATCGGACACTCTGAACGTCGTGAGTATTTCCACGAAACAGACGA
AGATATTAACAAAAAAGCACATGCAATCTTCAAACACGGCATGACACCAATCATTTGCTGTGGTGAAACATTAGATCAAC
GTGAAGCTGGTCAAACAGATACTTGGGTACGTGGTCAAATCCGTGCAGCTCTTGCTGGATTAACGGAAGAACAAGTAATC
AAATCTGTTATTGCTTATGAACCAATTTGGGCAATCGGTACTGGGAAATCTTCCACAAGTGCTGATGCAAACGAAACTTG
TGCAGTTATTCGTGCAGAAGTTGCAGACGCTGTATCTCAAAAAGCAGCAGACGCAGTTCGTATTCAATACGGCGGTAGCG
TAAAACCTGAAAACATTGCTGATTATCTTGCAGAGTCCGACATTGACGGCGCTCTTGTAGGTGGAGCAAGCTTAGAACCA
GCATCGTTCTTAGCATTATTGGAGGCAGTAAAATAA

Upstream 100 bases:

>100_bases
AGAATATCTTGAAGGTAAAGAGCTTCCTGGCGTTGCTTCTATTAGCGACAAATAAGCTGAACCAAAAATAATTTTTAATA
TCGAGAGGATGTTTATCCAA

Downstream 100 bases:

>100_bases
AATGAGTAAATCACCTGTAGCAATTATTATCCTCGATGGTTTTGGTAAACGTGCAGAAACAGTAGGTAATGCTGTAGCTC
AAGCAAACAAACCAAATTTC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 1; Triose-phosphate isomerase 1

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRKPIIAGNWKMNKTAAKAGQFAEDVKNNVPSSDAVESVVAAPALFLQELVRLTEGTNLRVAAQNCYFEDEGAFTGEISP
FALADLGVSYVIIGHSERREYFHETDEDINKKAHAIFKHGMTPIICCGETLDQREAGQTDTWVRGQIRAALAGLTEEQVI
KSVIAYEPIWAIGTGKSSTSADANETCAVIRAEVADAVSQKAADAVRIQYGGSVKPENIADYLAESDIDGALVGGASLEP
ASFLALLEAVK

Sequences:

>Translated_251_residues
MRKPIIAGNWKMNKTAAKAGQFAEDVKNNVPSSDAVESVVAAPALFLQELVRLTEGTNLRVAAQNCYFEDEGAFTGEISP
FALADLGVSYVIIGHSERREYFHETDEDINKKAHAIFKHGMTPIICCGETLDQREAGQTDTWVRGQIRAALAGLTEEQVI
KSVIAYEPIWAIGTGKSSTSADANETCAVIRAEVADAVSQKAADAVRIQYGGSVKPENIADYLAESDIDGALVGGASLEP
ASFLALLEAVK
>Mature_251_residues
MRKPIIAGNWKMNKTAAKAGQFAEDVKNNVPSSDAVESVVAAPALFLQELVRLTEGTNLRVAAQNCYFEDEGAFTGEISP
FALADLGVSYVIIGHSERREYFHETDEDINKKAHAIFKHGMTPIICCGETLDQREAGQTDTWVRGQIRAALAGLTEEQVI
KSVIAYEPIWAIGTGKSSTSADANETCAVIRAEVADAVSQKAADAVRIQYGGSVKPENIADYLAESDIDGALVGGASLEP
ASFLALLEAVK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=40.7258064516129, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI226529917, Length=248, Percent_Identity=40.7258064516129, Blast_Score=171, Evalue=7e-43,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=41.7670682730924, Blast_Score=193, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=43.7751004016064, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320255, Length=239, Percent_Identity=40.5857740585774, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI28572008, Length=248, Percent_Identity=41.1290322580645, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI28572006, Length=248, Percent_Identity=41.1290322580645, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI28572004, Length=248, Percent_Identity=41.1290322580645, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS1_LISIN (Q928I1)

Other databases:

- EMBL:   AL596172
- PIR:   AB1751
- RefSeq:   NP_471881.1
- ProteinModelPortal:   Q928I1
- SMR:   Q928I1
- GeneID:   1131363
- GenomeReviews:   AL592022_GR
- KEGG:   lin:lin2551
- GenoList:   LIN2551
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   LINN272626:LIN2551-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26861; Mature: 26861

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPIIAGNWKMNKTAAKAGQFAEDVKNNVPSSDAVESVVAAPALFLQELVRLTEGTNLR
CCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VAAQNCYFEDEGAFTGEISPFALADLGVSYVIIGHSERREYFHETDEDINKKAHAIFKHG
EEECCCEECCCCCEECCCCCHHHHHCCEEEEEEECHHHHHHHHCCHHHHHHHHHHHHHCC
MTPIICCGETLDQREAGQTDTWVRGQIRAALAGLTEEQVIKSVIAYEPIWAIGTGKSSTS
CCEEEECCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCC
ADANETCAVIRAEVADAVSQKAADAVRIQYGGSVKPENIADYLAESDIDGALVGGASLEP
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCCH
ASFLALLEAVK
HHHHHHHHHCC
>Mature Secondary Structure
MRKPIIAGNWKMNKTAAKAGQFAEDVKNNVPSSDAVESVVAAPALFLQELVRLTEGTNLR
CCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VAAQNCYFEDEGAFTGEISPFALADLGVSYVIIGHSERREYFHETDEDINKKAHAIFKHG
EEECCCEECCCCCEECCCCCHHHHHCCEEEEEEECHHHHHHHHCCHHHHHHHHHHHHHCC
MTPIICCGETLDQREAGQTDTWVRGQIRAALAGLTEEQVIKSVIAYEPIWAIGTGKSSTS
CCEEEECCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCC
ADANETCAVIRAEVADAVSQKAADAVRIQYGGSVKPENIADYLAESDIDGALVGGASLEP
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCCH
ASFLALLEAVK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669