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The map label for this gene is est [H]

Identifier: 226224998

GI number: 226224998

Start: 2495361

End: 2496107

Strand: Reverse

Name: est [H]

Synonym: Lm4b_02419

Alternate gene names: 226224998

Gene position: 2496107-2495361 (Counterclockwise)

Preceding gene: 226224999

Following gene: 226224997

Centisome position: 85.7

GC content: 39.09

Gene sequence:

>747_bases
ATGAAAATAACACCACCACAACCATTCCTATTCGAAAAAGGCAAAAGAGCAGTGTTACTCTTACACGGTTTCACAGGTAG
CTCAGCGGATGTAAGAATATTAGGTAGGTTTTTACAAGAAAACGATTATACTTGCTATGCGCCTCAATACAGAGGCCACG
GTGTATCTCCTGACCTACTGTTAAAAACGGGACCAAACGATTGGTGGGAAGATGTTCTTGAAGCCTATGATCACCTAAAA
TCACTCGGTTACACGGAAATCGCTGTAGCGGGACTTTCGCTAGGTGGACTTTTTTCACTAAAATTAGGTTTTTCTAGGCC
GTTAAAGGGAATTATAGCCATGAGTACTCCAACAAGAATGGACAGTTCATCACCAATAATTCAAGGCTTTTTAGATTATG
TGCGTAATTATAAAAAATTAGAAGGTAAAACACCTGAACAAATCGATGCTGAAATGGTTGCCTATAAAGACGCCCCAATG
AACACAATCGCTAAACTAAAGGACGAGATAAATGGCGTCGTGGCTGAAATAGATATGATTTATGCGCCAATAATGGTCGT
CCAAGGTGAAAAAGACGATATGGTTGATGTGAGCGGAGCACAGCTAATTTATGATACAGTAGAGTCAACGAAGAAAGAAT
TGCACTGGTTTAAAGAATCTGGGCACGTCATCACATTAGACAAAGAACGAAAAGAAGTAAATCAAGCCATTTTAACATTT
TTAGATAGTTTAGATTGGCAAGAATAG

Upstream 100 bases:

>100_bases
TAAATTATTAAATACAGCCAATCCCCTAGAACTCCAACAAAAATAACATCCAACCCAGATCAACCAAACCTCCAAAAAGA
ACCAAGGAGCGATATTTACT

Downstream 100 bases:

>100_bases
ACAATTTTTAAAACAGAGAGAGGAGGGACAGAAGTGGAACAAAAACAAATGGAAGAAAAAATTATGAATCTATTAACTTC
AGCGCCTGATAAAACATTTG

Product: carboxylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKITPPQPFLFEKGKRAVLLLHGFTGSSADVRILGRFLQENDYTCYAPQYRGHGVSPDLLLKTGPNDWWEDVLEAYDHLK
SLGYTEIAVAGLSLGGLFSLKLGFSRPLKGIIAMSTPTRMDSSSPIIQGFLDYVRNYKKLEGKTPEQIDAEMVAYKDAPM
NTIAKLKDEINGVVAEIDMIYAPIMVVQGEKDDMVDVSGAQLIYDTVESTKKELHWFKESGHVITLDKERKEVNQAILTF
LDSLDWQE

Sequences:

>Translated_248_residues
MKITPPQPFLFEKGKRAVLLLHGFTGSSADVRILGRFLQENDYTCYAPQYRGHGVSPDLLLKTGPNDWWEDVLEAYDHLK
SLGYTEIAVAGLSLGGLFSLKLGFSRPLKGIIAMSTPTRMDSSSPIIQGFLDYVRNYKKLEGKTPEQIDAEMVAYKDAPM
NTIAKLKDEINGVVAEIDMIYAPIMVVQGEKDDMVDVSGAQLIYDTVESTKKELHWFKESGHVITLDKERKEVNQAILTF
LDSLDWQE
>Mature_248_residues
MKITPPQPFLFEKGKRAVLLLHGFTGSSADVRILGRFLQENDYTCYAPQYRGHGVSPDLLLKTGPNDWWEDVLEAYDHLK
SLGYTEIAVAGLSLGGLFSLKLGFSRPLKGIIAMSTPTRMDSSSPIIQGFLDYVRNYKKLEGKTPEQIDAEMVAYKDAPM
NTIAKLKDEINGVVAEIDMIYAPIMVVQGEKDDMVDVSGAQLIYDTVESTKKELHWFKESGHVITLDKERKEVNQAILTF
LDSLDWQE

Specific function: Involved in the detoxification of xenobiotics. Shows maximal activity with C6 substrates, with gradually decreasing activity from C8 to C12 substrates. No activity for higher chain length substrates acids rather than long-chain ones [H]

COG id: COG1647

COG function: function code R; Esterase/lipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipase/esterase LIP3/BchO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019149
- InterPro:   IPR012354 [H]

Pfam domain/function: PF09752 DUF2048 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 27850; Mature: 27850

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITPPQPFLFEKGKRAVLLLHGFTGSSADVRILGRFLQENDYTCYAPQYRGHGVSPDLL
CCCCCCCCHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCEE
LKTGPNDWWEDVLEAYDHLKSLGYTEIAVAGLSLGGLFSLKLGFSRPLKGIIAMSTPTRM
EECCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCC
DSSSPIIQGFLDYVRNYKKLEGKTPEQIDAEMVAYKDAPMNTIAKLKDEINGVVAEIDMI
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHH
YAPIMVVQGEKDDMVDVSGAQLIYDTVESTKKELHWFKESGHVITLDKERKEVNQAILTF
HHCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHH
LDSLDWQE
HHHCCCCC
>Mature Secondary Structure
MKITPPQPFLFEKGKRAVLLLHGFTGSSADVRILGRFLQENDYTCYAPQYRGHGVSPDLL
CCCCCCCCHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCEE
LKTGPNDWWEDVLEAYDHLKSLGYTEIAVAGLSLGGLFSLKLGFSRPLKGIIAMSTPTRM
EECCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCC
DSSSPIIQGFLDYVRNYKKLEGKTPEQIDAEMVAYKDAPMNTIAKLKDEINGVVAEIDMI
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHH
YAPIMVVQGEKDDMVDVSGAQLIYDTVESTKKELHWFKESGHVITLDKERKEVNQAILTF
HHCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHH
LDSLDWQE
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]