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The map label for this gene is adaB [H]

Identifier: 226224850

GI number: 226224850

Start: 2339320

End: 2339802

Strand: Reverse

Name: adaB [H]

Synonym: Lm4b_02269

Alternate gene names: 226224850

Gene position: 2339802-2339320 (Counterclockwise)

Preceding gene: 226224853

Following gene: 226224849

Centisome position: 80.33

GC content: 38.3

Gene sequence:

>483_bases
ATGACAGATTTATATTATGATACGCTTCATTTTTCAGACACAGAGCTTTATTTCGCAGCGACGAAAACTGGGCTATTTTA
CGTTGGAACAAAGGCAGAAAAGATTCAATCAGCCAAGCGAAGCCCAGAAAAAATGAACATGTATAAAGAAGAATTGGTCG
CTTATTTGAACGGAGAACTTACGTATTTTACTGTACCAATGGATTTGTCCGCTACTCCTCTCCAACTTGAAGTATTTCAC
GCATTGGAAGCTATTCCTTATGGAGAAACTAGAACGTATACAGAAATTGCAGCACAGATAAATCGCCCCAAAGCCGTGCG
CGCAGTTGGAACGGCAATCGGAAGAAACCCACTACTTTTCATTATTCCATGCCACAGAGTAATTGGTAAAAATGGCAAAT
TAACAGGTTATAGCGGGGGGATAGGGATGAAAGAATCACTCTTAAAACTTGAAAAAGCAAAAATAAATCAATTTTCTTTT
TAA

Upstream 100 bases:

>100_bases
AATTGCGAGGAAAACTTTTCTAATCTTGCTCTTATAGTGAAACTGGTTAGAAATGTTATTCAATTTAGCGTATAATTAAC
ATTAGAAAGAGGTGAAGGAA

Downstream 100 bases:

>100_bases
AAAGAGAATTGATTTATTTTTTTATCTACTGTACTATAGTAACTAGTACAATTAGTTCTCTATAGTAGAATTCATGAAAG
CAGGGGGAGAGATGTTTACG

Product: O6-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTDLYYDTLHFSDTELYFAATKTGLFYVGTKAEKIQSAKRSPEKMNMYKEELVAYLNGELTYFTVPMDLSATPLQLEVFH
ALEAIPYGETRTYTEIAAQINRPKAVRAVGTAIGRNPLLFIIPCHRVIGKNGKLTGYSGGIGMKESLLKLEKAKINQFSF

Sequences:

>Translated_160_residues
MTDLYYDTLHFSDTELYFAATKTGLFYVGTKAEKIQSAKRSPEKMNMYKEELVAYLNGELTYFTVPMDLSATPLQLEVFH
ALEAIPYGETRTYTEIAAQINRPKAVRAVGTAIGRNPLLFIIPCHRVIGKNGKLTGYSGGIGMKESLLKLEKAKINQFSF
>Mature_159_residues
TDLYYDTLHFSDTELYFAATKTGLFYVGTKAEKIQSAKRSPEKMNMYKEELVAYLNGELTYFTVPMDLSATPLQLEVFHA
LEAIPYGETRTYTEIAAQINRPKAVRAVGTAIGRNPLLFIIPCHRVIGKNGKLTGYSGGIGMKESLLKLEKAKINQFSF

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=80, Percent_Identity=40, Blast_Score=70, Evalue=7e-13,
Organism=Escherichia coli, GI1788542, Length=106, Percent_Identity=44.3396226415094, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=46.6019417475728, Blast_Score=101, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=89, Percent_Identity=49.438202247191, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=89, Percent_Identity=49.438202247191, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320001, Length=89, Percent_Identity=42.6966292134831, Blast_Score=80, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137554, Length=90, Percent_Identity=43.3333333333333, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17942; Mature: 17811

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLYYDTLHFSDTELYFAATKTGLFYVGTKAEKIQSAKRSPEKMNMYKEELVAYLNGEL
CCCEEEEEEECCCCEEEEEEECCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCE
TYFTVPMDLSATPLQLEVFHALEAIPYGETRTYTEIAAQINRPKAVRAVGTAIGRNPLLF
EEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEE
IIPCHRVIGKNGKLTGYSGGIGMKESLLKLEKAKINQFSF
EEECHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDLYYDTLHFSDTELYFAATKTGLFYVGTKAEKIQSAKRSPEKMNMYKEELVAYLNGEL
CCEEEEEEECCCCEEEEEEECCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCE
TYFTVPMDLSATPLQLEVFHALEAIPYGETRTYTEIAAQINRPKAVRAVGTAIGRNPLLF
EEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEE
IIPCHRVIGKNGKLTGYSGGIGMKESLLKLEKAKINQFSF
EEECHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]