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The map label for this gene is pdxT

Identifier: 226224707

GI number: 226224707

Start: 2181852

End: 2182418

Strand: Direct

Name: pdxT

Synonym: Lm4b_02123

Alternate gene names: 226224707

Gene position: 2181852-2182418 (Clockwise)

Preceding gene: 226224706

Following gene: 226224709

Centisome position: 74.91

GC content: 40.21

Gene sequence:

>567_bases
ATGAAAAAAATTGGAGTCCTTGCAATTCAAGGTGCAGTTGATGAACATATCCAAATGATTGAATCAGCCGGTGCTCTTGC
TTTTAAAGTAAAGCATTCAAGTGATTTAGATGGACTTGACGGGCTTGTTTTACCTGGTGGTGAAAGTACAACGATGCGCA
AGATTATGAAGCGTTATGATTTAATGGAACCTATCCGCGCATTTGCAAGTGAAGGGAAAGCTATTTTTGGAACTTGTGCT
GGGCTTGTCCTTTTGTCAAAAGAAATTGAAGGTGGCGAAGAGAGCCTAGGATTGATTGAAGCTACCGCGATCCGTAATGG
TTTTGGTAGGCAGAAAGAGAGTTTTGAAGCCGAATTAAACATCGAAGCATTTGGTGAACCTGCGTTTGAAGCTATATTTA
TCCGCGCACCATACTTAATTGAACCGAGTAATGAGGTAGCTGTGTTAGCAACAGTTGAAAATCGAATCGTAGCAGCTAAA
CAAGCTAATATTTTAGTTACCGCATTCCATCCTGAACTTACTAACGACAATCGCTGGATGAATTACTTCCTCGAAAAAAT
GGTATAA

Upstream 100 bases:

>100_bases
CTAATCGGAAAACTTTCCAAAGAGCTAGGTTCCCCGATGAAAGGAATCGAAATGTCTCGCCTTAACCCAGAAGACAGAAT
GCAAGATCGGAGCATTTAAT

Downstream 100 bases:

>100_bases
AAAAAGCAGTAGACAATTTAGTCTACTGCTTTTGCATATTATTTATTCACTGCTTGCGCTGCTGTAATTAACGTTAAGTT
ATAAACGTCATCTGTATTAC

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MKKIGVLAIQGAVDEHIQMIESAGALAFKVKHSSDLDGLDGLVLPGGESTTMRKIMKRYDLMEPIRAFASEGKAIFGTCA
GLVLLSKEIEGGEESLGLIEATAIRNGFGRQKESFEAELNIEAFGEPAFEAIFIRAPYLIEPSNEVAVLATVENRIVAAK
QANILVTAFHPELTNDNRWMNYFLEKMV

Sequences:

>Translated_188_residues
MKKIGVLAIQGAVDEHIQMIESAGALAFKVKHSSDLDGLDGLVLPGGESTTMRKIMKRYDLMEPIRAFASEGKAIFGTCA
GLVLLSKEIEGGEESLGLIEATAIRNGFGRQKESFEAELNIEAFGEPAFEAIFIRAPYLIEPSNEVAVLATVENRIVAAK
QANILVTAFHPELTNDNRWMNYFLEKMV
>Mature_188_residues
MKKIGVLAIQGAVDEHIQMIESAGALAFKVKHSSDLDGLDGLVLPGGESTTMRKIMKRYDLMEPIRAFASEGKAIFGTCA
GLVLLSKEIEGGEESLGLIEATAIRNGFGRQKESFEAELNIEAFGEPAFEAIFIRAPYLIEPSNEVAVLATVENRIVAAK
QANILVTAFHPELTNDNRWMNYFLEKMV

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=210, Percent_Identity=38.5714285714286, Blast_Score=120, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6323995, Length=213, Percent_Identity=35.6807511737089, Blast_Score=109, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321048, Length=213, Percent_Identity=35.6807511737089, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_LISMC (C1KX54)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002758814.1
- ProteinModelPortal:   C1KX54
- SMR:   C1KX54
- GeneID:   7703158
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_02123
- OMA:   QGDVREH
- ProtClustDB:   PRK13525
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 20574; Mature: 20574

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 79-79 ACT_SITE 170-170 ACT_SITE 172-172 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGVLAIQGAVDEHIQMIESAGALAFKVKHSSDLDGLDGLVLPGGESTTMRKIMKRYD
CCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHH
LMEPIRAFASEGKAIFGTCAGLVLLSKEIEGGEESLGLIEATAIRNGFGRQKESFEAELN
HHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCHHCCHHHHHHHHCCCCCCCCCCEEEEE
IEAFGEPAFEAIFIRAPYLIEPSNEVAVLATVENRIVAAKQANILVTAFHPELTNDNRWM
EEECCCCCEEEEEEECCEEECCCCCEEEEEEECCEEEEEECCCEEEEEECCCCCCCCHHH
NYFLEKMV
HHHHHHCC
>Mature Secondary Structure
MKKIGVLAIQGAVDEHIQMIESAGALAFKVKHSSDLDGLDGLVLPGGESTTMRKIMKRYD
CCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHH
LMEPIRAFASEGKAIFGTCAGLVLLSKEIEGGEESLGLIEATAIRNGFGRQKESFEAELN
HHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCHHCCHHHHHHHHCCCCCCCCCCEEEEE
IEAFGEPAFEAIFIRAPYLIEPSNEVAVLATVENRIVAAKQANILVTAFHPELTNDNRWM
EEECCCCCEEEEEEECCEEECCCCCEEEEEEECCEEEEEECCCEEEEEECCCCCCCCHHH
NYFLEKMV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA