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The map label for this gene is pflA

Identifier: 226224521

GI number: 226224521

Start: 1978911

End: 1981190

Strand: Direct

Name: pflA

Synonym: Lm4b_01934

Alternate gene names: 226224521

Gene position: 1978911-1981190 (Clockwise)

Preceding gene: 226224520

Following gene: 226224548

Centisome position: 67.94

GC content: 38.33

Gene sequence:

>2280_bases
ATGACAAAAGCATGGGATGGTTTTAAAGGAACCGCATGGCAAGAAAACATTAGTGTAGGGCAATTCGTTCAAGACAACTA
CACTCCTTACGATGGCGATGAAAGCTTTTTAGAAAAGAGCACACCAAGAACAACGAAACTAAATGAAAAAATAAATAAAC
TAGTAGAAGAAATGGATGCAAAAGGCGGCGTACTTGATATGGATAACGCTATCGTATCCAATGTTGCATCTCACAAAGCT
GGTTACGTGGATCAAGAAAATGAAGTTATTGTTGGTTTACAAACAGACAAACCATTCAAATTAGCATTTATGCCGAATGG
TGGTCTTAGAACAGCCGAACAATGTTTAACAGACAACGGCTATTCCATTGATCAAGAATTACATGACTTCTACGTAAAAA
ATCGTTCTACAGCAAATGATGGTATTTTCAGAGCATATACAGACGATATCAAACGTGCGCGTCACTCCCATATCGTTAGT
GGTCTTCCTGATGCATACTCTCGCGGAAGAATCATCGGCTTGTACCAAAAACCAGCACTTTACGGTGTGGATCGTTTAAT
AGCAGAAAAACAACAAGACTTGAAAAAAATTGCGATTTCTTCTGATGAAAACATTCGTTTACGTGAAGAAATTTGGTTAC
AAATCAAAGCACTTAAAGACTTAATCGTTTTAGGTAACGAATACGGTTTAGAACTTGGACGTCCAGCTGAAAATGCAACA
GAAGCGGTACAATGGACTTACATGGGCTACCTTGCTTCTATCAAACAAGCAAACGGTGCTGCAAGCTCATTCGGTCGTAT
TCCGATTTTCCTTGATATCTATATCCAACGCGATTTAGAAAAAGGCATTATCACTGAATTCGATGCACAAGAATTAATCG
AACAATTAACCCTTAAATTAAGAATGGTTCGTTTTGCAAGAACAGATGGTTACAACGAACTTTACGCTTCCAATCCAACA
TTCGTTACAACTTCAATGGCTGGTATGGGCGCAGACGGTCGTCATCGTGTGACTAAAACAGATTATCGTTTCTTACACTG
CTTAGATAACCTAGGAAACTCAGCAGAACCAAACTTAACTGTCCTATGGGATGCTCGTTTACCAGAAAGCTTTAAAGAAT
ATTGTATGAAAATGAGTGTAAAACATTCCTCCATTCAATACGAAAATGATAAATTAATGCAAGAAGAAGGCTACGGCGAT
ATGCAATGTATCAGCTGTTGTGTTAGCCCACTTAACCCAGAAGCAGACAAAGACAAAGGTGAAACACATAACCTGCAATA
TTTCGGTGCTCGTGTCAACGTACTGAAATGTCTTCTTGGAGCTATCAACGGCGGTAAAGATGATCTTCACAAAAACCAAG
TATTCGACGTTGTAGAACCAATTACAACCGAATACCTTGAATACGAAGAAGTACTAGAAAAATTCGATAAATCAATGGAC
TGGTTAACAGACACTTATGTAGATGCGATGAACATCATTCACTTTATGACAGATAAATACAACTATGAAAGTATGCAAAT
GGCCTTCTTACCATCTAAAGTAACAGCCAACATGGGCTTCGGTATTTGTGGATTCGCTAACGTAGTAGATAGCCTAAGTG
CCATCAAACATGCCAAAGTAAAAACTATCCGTGACGAAGATGGTTTTGTATATGACTATGAAGTAGAAGGCGACTTCCCT
CGTTACGGTGAAAATGATGACCGTGCCGACGATATCGCTGTAATGGTTCTTAAAATGTTCAAAGACAAATTAGACTCCCA
TAAACTATACAAAGATAGTGAAGCAACCGTTTCTGTTCTAACAATCACTTCTAACGTTGCTTACTCAAAACAATGTGGTA
ACTCCCCCGTGCACAAAGGACCTGTATTTGATGAAAATGGCAAAATCGTCAAAGAACCAGAATTCTTCAGCCCAGGAGCT
AACCCTTCTAACAAAGCAAAAGGTGGTTTCTTAGATAACCTTGCTAGTCTTTCTAAATTACCATTCCACTATGCAAATGA
CGGAATCTCCTTAACAATCCAAGGAGCTCCAAAAATGTTCGGTAAAACAACGGAAGAACAACACCATAACCTTGTTGGTA
TTCTGGATGGTTACTTTACAAAAGGTGGTCAACACATCAACTTAAACGTTCTTAACCACGATGAAGTAATCGAAAAAATC
AAAGCTGGTATCCCAGTAATCTTACGTATCAGCGGTTATTGCTTAAATACGAAAGATCTAAATGAAGAACAAAAAATGGA
ACTTTGCCAAAGAATGTTCCACGAAAAATTAATCGGTTAA

Upstream 100 bases:

>100_bases
TTCGTTTTCACAAAGGAGTATGCTGTTCAATGAAGAAAGATTCTAAAGACTTGTTTCTGGCTTTAAGTGAAAAGGAACAC
AATTGAAGGAGGAAATTATA

Downstream 100 bases:

>100_bases
TAAGAAAAAAAGAACCAAATCTGCGAATAACAGATTTGGTTCTTTTTTTATGCGTTCGAAACTTCCGCCGCATAAGCTCC
TGCCGTGTGCCCAGTCACTA

Product: pyruvate formate-lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 759; Mature: 758

Protein sequence:

>759_residues
MTKAWDGFKGTAWQENISVGQFVQDNYTPYDGDESFLEKSTPRTTKLNEKINKLVEEMDAKGGVLDMDNAIVSNVASHKA
GYVDQENEVIVGLQTDKPFKLAFMPNGGLRTAEQCLTDNGYSIDQELHDFYVKNRSTANDGIFRAYTDDIKRARHSHIVS
GLPDAYSRGRIIGLYQKPALYGVDRLIAEKQQDLKKIAISSDENIRLREEIWLQIKALKDLIVLGNEYGLELGRPAENAT
EAVQWTYMGYLASIKQANGAASSFGRIPIFLDIYIQRDLEKGIITEFDAQELIEQLTLKLRMVRFARTDGYNELYASNPT
FVTTSMAGMGADGRHRVTKTDYRFLHCLDNLGNSAEPNLTVLWDARLPESFKEYCMKMSVKHSSIQYENDKLMQEEGYGD
MQCISCCVSPLNPEADKDKGETHNLQYFGARVNVLKCLLGAINGGKDDLHKNQVFDVVEPITTEYLEYEEVLEKFDKSMD
WLTDTYVDAMNIIHFMTDKYNYESMQMAFLPSKVTANMGFGICGFANVVDSLSAIKHAKVKTIRDEDGFVYDYEVEGDFP
RYGENDDRADDIAVMVLKMFKDKLDSHKLYKDSEATVSVLTITSNVAYSKQCGNSPVHKGPVFDENGKIVKEPEFFSPGA
NPSNKAKGGFLDNLASLSKLPFHYANDGISLTIQGAPKMFGKTTEEQHHNLVGILDGYFTKGGQHINLNVLNHDEVIEKI
KAGIPVILRISGYCLNTKDLNEEQKMELCQRMFHEKLIG

Sequences:

>Translated_759_residues
MTKAWDGFKGTAWQENISVGQFVQDNYTPYDGDESFLEKSTPRTTKLNEKINKLVEEMDAKGGVLDMDNAIVSNVASHKA
GYVDQENEVIVGLQTDKPFKLAFMPNGGLRTAEQCLTDNGYSIDQELHDFYVKNRSTANDGIFRAYTDDIKRARHSHIVS
GLPDAYSRGRIIGLYQKPALYGVDRLIAEKQQDLKKIAISSDENIRLREEIWLQIKALKDLIVLGNEYGLELGRPAENAT
EAVQWTYMGYLASIKQANGAASSFGRIPIFLDIYIQRDLEKGIITEFDAQELIEQLTLKLRMVRFARTDGYNELYASNPT
FVTTSMAGMGADGRHRVTKTDYRFLHCLDNLGNSAEPNLTVLWDARLPESFKEYCMKMSVKHSSIQYENDKLMQEEGYGD
MQCISCCVSPLNPEADKDKGETHNLQYFGARVNVLKCLLGAINGGKDDLHKNQVFDVVEPITTEYLEYEEVLEKFDKSMD
WLTDTYVDAMNIIHFMTDKYNYESMQMAFLPSKVTANMGFGICGFANVVDSLSAIKHAKVKTIRDEDGFVYDYEVEGDFP
RYGENDDRADDIAVMVLKMFKDKLDSHKLYKDSEATVSVLTITSNVAYSKQCGNSPVHKGPVFDENGKIVKEPEFFSPGA
NPSNKAKGGFLDNLASLSKLPFHYANDGISLTIQGAPKMFGKTTEEQHHNLVGILDGYFTKGGQHINLNVLNHDEVIEKI
KAGIPVILRISGYCLNTKDLNEEQKMELCQRMFHEKLIG
>Mature_758_residues
TKAWDGFKGTAWQENISVGQFVQDNYTPYDGDESFLEKSTPRTTKLNEKINKLVEEMDAKGGVLDMDNAIVSNVASHKAG
YVDQENEVIVGLQTDKPFKLAFMPNGGLRTAEQCLTDNGYSIDQELHDFYVKNRSTANDGIFRAYTDDIKRARHSHIVSG
LPDAYSRGRIIGLYQKPALYGVDRLIAEKQQDLKKIAISSDENIRLREEIWLQIKALKDLIVLGNEYGLELGRPAENATE
AVQWTYMGYLASIKQANGAASSFGRIPIFLDIYIQRDLEKGIITEFDAQELIEQLTLKLRMVRFARTDGYNELYASNPTF
VTTSMAGMGADGRHRVTKTDYRFLHCLDNLGNSAEPNLTVLWDARLPESFKEYCMKMSVKHSSIQYENDKLMQEEGYGDM
QCISCCVSPLNPEADKDKGETHNLQYFGARVNVLKCLLGAINGGKDDLHKNQVFDVVEPITTEYLEYEEVLEKFDKSMDW
LTDTYVDAMNIIHFMTDKYNYESMQMAFLPSKVTANMGFGICGFANVVDSLSAIKHAKVKTIRDEDGFVYDYEVEGDFPR
YGENDDRADDIAVMVLKMFKDKLDSHKLYKDSEATVSVLTITSNVAYSKQCGNSPVHKGPVFDENGKIVKEPEFFSPGAN
PSNKAKGGFLDNLASLSKLPFHYANDGISLTIQGAPKMFGKTTEEQHHNLVGILDGYFTKGGQHINLNVLNHDEVIEKIK
AGIPVILRISGYCLNTKDLNEEQKMELCQRMFHEKLIG

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=773, Percent_Identity=44.372574385511, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=769, Percent_Identity=42.5227568270481, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=596, Percent_Identity=20.3020134228188, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85508; Mature: 85377

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAWDGFKGTAWQENISVGQFVQDNYTPYDGDESFLEKSTPRTTKLNEKINKLVEEMDA
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCC
KGGVLDMDNAIVSNVASHKAGYVDQENEVIVGLQTDKPFKLAFMPNGGLRTAEQCLTDNG
CCCEEECCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHCCCC
YSIDQELHDFYVKNRSTANDGIFRAYTDDIKRARHSHIVSGLPDAYSRGRIIGLYQKPAL
CCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCHH
YGVDRLIAEKQQDLKKIAISSDENIRLREEIWLQIKALKDLIVLGNEYGLELGRPAENAT
HHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHH
EAVQWTYMGYLASIKQANGAASSFGRIPIFLDIYIQRDLEKGIITEFDAQELIEQLTLKL
HHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHH
RMVRFARTDGYNELYASNPTFVTTSMAGMGADGRHRVTKTDYRFLHCLDNLGNSAEPNLT
HHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEE
VLWDARLPESFKEYCMKMSVKHSSIQYENDKLMQEEGYGDMQCISCCVSPLNPEADKDKG
EEECCCCCHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
ETHNLQYFGARVNVLKCLLGAINGGKDDLHKNQVFDVVEPITTEYLEYEEVLEKFDKSMD
CCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLTDTYVDAMNIIHFMTDKYNYESMQMAFLPSKVTANMGFGICGFANVVDSLSAIKHAKV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KTIRDEDGFVYDYEVEGDFPRYGENDDRADDIAVMVLKMFKDKLDSHKLYKDSEATVSVL
HEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
TITSNVAYSKQCGNSPVHKGPVFDENGKIVKEPEFFSPGANPSNKAKGGFLDNLASLSKL
EEECCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHC
PFHYANDGISLTIQGAPKMFGKTTEEQHHNLVGILDGYFTKGGQHINLNVLNHDEVIEKI
CCEEECCCEEEEECCCCHHHCCCCHHHHCCEEEEEHHHHCCCCCEEEEEEECHHHHHHHH
KAGIPVILRISGYCLNTKDLNEEQKMELCQRMFHEKLIG
HCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKAWDGFKGTAWQENISVGQFVQDNYTPYDGDESFLEKSTPRTTKLNEKINKLVEEMDA
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCC
KGGVLDMDNAIVSNVASHKAGYVDQENEVIVGLQTDKPFKLAFMPNGGLRTAEQCLTDNG
CCCEEECCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHCCCC
YSIDQELHDFYVKNRSTANDGIFRAYTDDIKRARHSHIVSGLPDAYSRGRIIGLYQKPAL
CCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCHH
YGVDRLIAEKQQDLKKIAISSDENIRLREEIWLQIKALKDLIVLGNEYGLELGRPAENAT
HHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHH
EAVQWTYMGYLASIKQANGAASSFGRIPIFLDIYIQRDLEKGIITEFDAQELIEQLTLKL
HHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHH
RMVRFARTDGYNELYASNPTFVTTSMAGMGADGRHRVTKTDYRFLHCLDNLGNSAEPNLT
HHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEE
VLWDARLPESFKEYCMKMSVKHSSIQYENDKLMQEEGYGDMQCISCCVSPLNPEADKDKG
EEECCCCCHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
ETHNLQYFGARVNVLKCLLGAINGGKDDLHKNQVFDVVEPITTEYLEYEEVLEKFDKSMD
CCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLTDTYVDAMNIIHFMTDKYNYESMQMAFLPSKVTANMGFGICGFANVVDSLSAIKHAKV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KTIRDEDGFVYDYEVEGDFPRYGENDDRADDIAVMVLKMFKDKLDSHKLYKDSEATVSVL
HEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
TITSNVAYSKQCGNSPVHKGPVFDENGKIVKEPEFFSPGANPSNKAKGGFLDNLASLSKL
EEECCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHC
PFHYANDGISLTIQGAPKMFGKTTEEQHHNLVGILDGYFTKGGQHINLNVLNHDEVIEKI
CCEEECCCEEEEECCCCHHHCCCCHHHHCCEEEEEHHHHCCCCCEEEEEEECHHHHHHHH
KAGIPVILRISGYCLNTKDLNEEQKMELCQRMFHEKLIG
HCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550181; 12397186 [H]