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The map label for this gene is pbpA

Identifier: 226224496

GI number: 226224496

Start: 1952353

End: 1954836

Strand: Direct

Name: pbpA

Synonym: Lm4b_01909

Alternate gene names: 226224496

Gene position: 1952353-1954836 (Clockwise)

Preceding gene: 226224495

Following gene: 226224513

Centisome position: 67.03

GC content: 39.77

Gene sequence:

>2484_bases
ATGGCAGATAAACCGCAGACAAGATCTCAGTATCGCAATAAACAAAGTGGTGGTTCTAAAAAGAAATCCCAAAAACGAGG
AAAACGAGTAGTAGCGAATATTTTCAAAACTATTTTCTTTGTGGGATTATTTTTAGCTTTCTTTGGTATTGCGGCTGGTG
CAACTGTCTTTTACGATTACGCAAAAGATGCACCAAAACTGACCGACTCCAAGCTACGAGATCCACTTTCATCTAAGTTA
CTTGATAAAGATGGAAAAGTTTTCGCAGAAGTTGGAACCGAACGGCGGGAATATATTGAATACAAAGATATACCTGAAAC
ACTAAAAAATGCAATTCTAACAACGGAAGATGCGCGTTTTTATGAACATGATGGCATTGACCCAATTCGACTTGGTGGAG
CAGTAATTGCCAATTTGACAGACGGTTTTGGCGCAGAAGGTGCAAGTACCCTTTCGCAACAAATCATCAAAATGTCCTAT
CTTGATTACACAAATAAAACACTTGCAAGAAAAGCTCAAGAAGCATGGTTAGCTCTTCAATTAGAAGAAAAATATAGCAA
GAATGACATCTTAGAAATTTATGTTAATAAAGTCTATATGTCAGACCGTGTCCACGGCATGCAAACAGCTTCCGAACATT
ACTTCGGTAAAAATTTGAAAGACCTTACGTTAGCTCAAACAGCCCTACTTGCAGGTATGCCACAAAGTCCGAATAACTAC
AATCCTTATGAACACCCAGAAGCAGCAAAAAAACGTCGTGATCAAGTTTTAACAAATATGTATACACATGATAAAATTAC
AAAAGAAGAAATGACCGAAGCTCAAAAAACATCTATCACTACCGGACTTCGCTCGAAAAAAGATCGTGAAGATAAAATTT
ACAAATACGATTCTTACGTCACACAAGTGTTAAGTGAAATTCCAAAAGAATACGATGTTTATCGTGATGGTTTAACTATC
CATACAGCGCTTGACCGTGATGCCCAAGAATATACGGAGAAAATGCTCAATACGAACGAAATTGTTAACTTCACTGATGA
TGAAATGCAAGCCGGTATAGTGCTTCAAGATACTAAAACTGGACGTGTTCAAGCAATCGGAGGCGGACGTAAACAAAAAG
TAACTCGGGGATATAACTATGCAACCCAAGTAAAACGTTCTGTTGGGTCTACCATGAAACCAATTGCTGACTATGGTCCT
GCTTTTGAATATTTGGATTGGTCGACTGCCCATATTTTAGAAGATGAACCATATACGTATTCTGGCGGAACCCCTATTAA
CAACTGGGATTTCGCATACAAAGGACCAATTTCAGTAAGACAAGCACTTTACCAATCACGTAATATTCCAGCCCTAAAAA
CCCTTCAAGCTGTTGGGCTAGATAAATCCGAGGAATTTGTTAATAAACTTGGCATCACCTATGATGAAGGACAAAACGTA
GAATCCAACGCTATTGGAGCAAACAGTTCCAACCCAATGCAAATGGCTGGTGCCTATGCTGCATTCGGTAACAAAGGAAT
CTATAACAAGCCACACACTGTTACAAAAATCGTTTTATCAGATGGACAAACAGAAATTGACACAGAACCACAAAGTACTG
TTGCTATGAAAGAATCAACTGCTTATATGGTTTCAGATGTTCTAAAAGATGTTCTTTCTATCGGAACAGGTACATCAGCA
GCCGTACCAGGCGTACCTGCTGCTGGTAAAACAGGTACAACGAATATTCCACCTGAATTCACTTCGAAGTACTACTACCC
TAGTGGTGCTGCCCGTGACTCATGGTTCGCTGGATATACAACCAATTATTCGATTGCTGTATGGACCGGCTATGATGACA
AGAAAAAATATGTTTCAGCAAGTGAACAAAAAATTGCGCAGCGGATGTTTAGTAAATTAATGGCTCATGCATCTGCCGGT
AAAACTACTGCAGATTTCAAAATGCCTAGCAATGTTGTTTCCGTCCCAATTCTAAAAGGAAGCAACCCAATTGCACGCGC
TGCTGCAGGCACTGCAAGTGATAAAGTAAGTTACGAATTATTCTTATCCGGAACCGCTCCAACCAAAACTGCTTCTACTC
CAGAAGACGAGAAGAAAAAAGCAGAAGAAGCTGCGAAGAAGAAAAAAGCGGAAGAAGATAAAAAGAAAACAGATGAAGAG
AAGAAAAAAGAAGAAGAGGCTAAGAAGAAAGCAGAAGAGGAAGCTAAGAAAAAGGCTGAAGAAGAAGCCAAAAATCTTAC
TGCCCCTGCCGGCTTAAGAGCAAGTTATAATGCCGGCTCTAAGCAAATCAATGTTTCTTGGTCTGCGGTAGATGGTGCAA
CCTATGAAGTAACAGTCAATGGTTCTACTACAACAGTATCTTCTACTTCCGTTTCAGTAAGCGGAGGTAATCCTGGAGAC
ACTGTTTCCATTAACGTCGTCGCTGTTAAAGACGGTAATCGAAGCCCTGCCTCCTCTACTACAGTTAAAATTCCAGATAG
TTAA

Upstream 100 bases:

>100_bases
GACCAAATTCCTTATGGTCTAAATCCAAGGCTTGACTTCTTGCAATCCATAGACAAATTATACTTCTAGTCAAACCTTTT
TTAAAGGAGAGAGATTTTTA

Downstream 100 bases:

>100_bases
TACAAAAAGAGCGGAAGATGTTTAAACACCTTCCGCTCTTTTTTATGATCTTTTCGCAATTAATTTACGCTGTTCTAACA
TTAATTCATTTAATTGCCGA

Product: penicillin-binding protein 2A

Products: NA

Alternate protein names: PBP1; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 827; Mature: 826

Protein sequence:

>827_residues
MADKPQTRSQYRNKQSGGSKKKSQKRGKRVVANIFKTIFFVGLFLAFFGIAAGATVFYDYAKDAPKLTDSKLRDPLSSKL
LDKDGKVFAEVGTERREYIEYKDIPETLKNAILTTEDARFYEHDGIDPIRLGGAVIANLTDGFGAEGASTLSQQIIKMSY
LDYTNKTLARKAQEAWLALQLEEKYSKNDILEIYVNKVYMSDRVHGMQTASEHYFGKNLKDLTLAQTALLAGMPQSPNNY
NPYEHPEAAKKRRDQVLTNMYTHDKITKEEMTEAQKTSITTGLRSKKDREDKIYKYDSYVTQVLSEIPKEYDVYRDGLTI
HTALDRDAQEYTEKMLNTNEIVNFTDDEMQAGIVLQDTKTGRVQAIGGGRKQKVTRGYNYATQVKRSVGSTMKPIADYGP
AFEYLDWSTAHILEDEPYTYSGGTPINNWDFAYKGPISVRQALYQSRNIPALKTLQAVGLDKSEEFVNKLGITYDEGQNV
ESNAIGANSSNPMQMAGAYAAFGNKGIYNKPHTVTKIVLSDGQTEIDTEPQSTVAMKESTAYMVSDVLKDVLSIGTGTSA
AVPGVPAAGKTGTTNIPPEFTSKYYYPSGAARDSWFAGYTTNYSIAVWTGYDDKKKYVSASEQKIAQRMFSKLMAHASAG
KTTADFKMPSNVVSVPILKGSNPIARAAAGTASDKVSYELFLSGTAPTKTASTPEDEKKKAEEAAKKKKAEEDKKKTDEE
KKKEEEAKKKAEEEAKKKAEEEAKNLTAPAGLRASYNAGSKQINVSWSAVDGATYEVTVNGSTTTVSSTSVSVSGGNPGD
TVSINVVAVKDGNRSPASSTTVKIPDS

Sequences:

>Translated_827_residues
MADKPQTRSQYRNKQSGGSKKKSQKRGKRVVANIFKTIFFVGLFLAFFGIAAGATVFYDYAKDAPKLTDSKLRDPLSSKL
LDKDGKVFAEVGTERREYIEYKDIPETLKNAILTTEDARFYEHDGIDPIRLGGAVIANLTDGFGAEGASTLSQQIIKMSY
LDYTNKTLARKAQEAWLALQLEEKYSKNDILEIYVNKVYMSDRVHGMQTASEHYFGKNLKDLTLAQTALLAGMPQSPNNY
NPYEHPEAAKKRRDQVLTNMYTHDKITKEEMTEAQKTSITTGLRSKKDREDKIYKYDSYVTQVLSEIPKEYDVYRDGLTI
HTALDRDAQEYTEKMLNTNEIVNFTDDEMQAGIVLQDTKTGRVQAIGGGRKQKVTRGYNYATQVKRSVGSTMKPIADYGP
AFEYLDWSTAHILEDEPYTYSGGTPINNWDFAYKGPISVRQALYQSRNIPALKTLQAVGLDKSEEFVNKLGITYDEGQNV
ESNAIGANSSNPMQMAGAYAAFGNKGIYNKPHTVTKIVLSDGQTEIDTEPQSTVAMKESTAYMVSDVLKDVLSIGTGTSA
AVPGVPAAGKTGTTNIPPEFTSKYYYPSGAARDSWFAGYTTNYSIAVWTGYDDKKKYVSASEQKIAQRMFSKLMAHASAG
KTTADFKMPSNVVSVPILKGSNPIARAAAGTASDKVSYELFLSGTAPTKTASTPEDEKKKAEEAAKKKKAEEDKKKTDEE
KKKEEEAKKKAEEEAKKKAEEEAKNLTAPAGLRASYNAGSKQINVSWSAVDGATYEVTVNGSTTTVSSTSVSVSGGNPGD
TVSINVVAVKDGNRSPASSTTVKIPDS
>Mature_826_residues
ADKPQTRSQYRNKQSGGSKKKSQKRGKRVVANIFKTIFFVGLFLAFFGIAAGATVFYDYAKDAPKLTDSKLRDPLSSKLL
DKDGKVFAEVGTERREYIEYKDIPETLKNAILTTEDARFYEHDGIDPIRLGGAVIANLTDGFGAEGASTLSQQIIKMSYL
DYTNKTLARKAQEAWLALQLEEKYSKNDILEIYVNKVYMSDRVHGMQTASEHYFGKNLKDLTLAQTALLAGMPQSPNNYN
PYEHPEAAKKRRDQVLTNMYTHDKITKEEMTEAQKTSITTGLRSKKDREDKIYKYDSYVTQVLSEIPKEYDVYRDGLTIH
TALDRDAQEYTEKMLNTNEIVNFTDDEMQAGIVLQDTKTGRVQAIGGGRKQKVTRGYNYATQVKRSVGSTMKPIADYGPA
FEYLDWSTAHILEDEPYTYSGGTPINNWDFAYKGPISVRQALYQSRNIPALKTLQAVGLDKSEEFVNKLGITYDEGQNVE
SNAIGANSSNPMQMAGAYAAFGNKGIYNKPHTVTKIVLSDGQTEIDTEPQSTVAMKESTAYMVSDVLKDVLSIGTGTSAA
VPGVPAAGKTGTTNIPPEFTSKYYYPSGAARDSWFAGYTTNYSIAVWTGYDDKKKYVSASEQKIAQRMFSKLMAHASAGK
TTADFKMPSNVVSVPILKGSNPIARAAAGTASDKVSYELFLSGTAPTKTASTPEDEKKKAEEAAKKKKAEEDKKKTDEEK
KKEEEAKKKAEEEAKKKAEEEAKNLTAPAGLRASYNAGSKQINVSWSAVDGATYEVTVNGSTTTVSSTSVSVSGGNPGDT
VSINVVAVKDGNRSPASSTTVKIPDS

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=563, Percent_Identity=30.7282415630551, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI87082258, Length=320, Percent_Identity=30.9375, Blast_Score=170, Evalue=4e-43,
Organism=Escherichia coli, GI1788867, Length=583, Percent_Identity=26.5866209262436, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=30.9090909090909, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR001264
- InterPro:   IPR013783
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00041 fn3; PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 90822; Mature: 90691

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS50853 FN3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKPQTRSQYRNKQSGGSKKKSQKRGKRVVANIFKTIFFVGLFLAFFGIAAGATVFYDY
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEHH
AKDAPKLTDSKLRDPLSSKLLDKDGKVFAEVGTERREYIEYKDIPETLKNAILTTEDARF
HCCCCCCCHHHHHCHHHHHHHCCCCCCHHHHCCHHHHHHCCCCHHHHHHHHEECCCCCCH
YEHDGIDPIRLGGAVIANLTDGFGAEGASTLSQQIIKMSYLDYTNKTLARKAQEAWLALQ
HHCCCCCEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
LEEKYSKNDILEIYVNKVYMSDRVHGMQTASEHYFGKNLKDLTLAQTALLAGMPQSPNNY
EHHHCCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
NPYEHPEAAKKRRDQVLTNMYTHDKITKEEMTEAQKTSITTGLRSKKDREDKIYKYDSYV
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TQVLSEIPKEYDVYRDGLTIHTALDRDAQEYTEKMLNTNEIVNFTDDEMQAGIVLQDTKT
HHHHHHCCHHHHHHHCCEEEEEHHCCCHHHHHHHHCCCCCEEECCCCHHHCCEEEEECCC
GRVQAIGGGRKQKVTRGYNYATQVKRSVGSTMKPIADYGPAFEYLDWSTAHILEDEPYTY
CEEEEECCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCHHHCCCCHHEEECCCCEEE
SGGTPINNWDFAYKGPISVRQALYQSRNIPALKTLQAVGLDKSEEFVNKLGITYDEGQNV
CCCCCCCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCEECCCCCC
ESNAIGANSSNPMQMAGAYAAFGNKGIYNKPHTVTKIVLSDGQTEIDTEPQSTVAMKEST
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHEEHHHHH
AYMVSDVLKDVLSIGTGTSAAVPGVPAAGKTGTTNIPPEFTSKYYYPSGAARDSWFAGYT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCEECEE
TNYSIAVWTGYDDKKKYVSASEQKIAQRMFSKLMAHASAGKTTADFKMPSNVVSVPILKG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
SNPIARAAAGTASDKVSYELFLSGTAPTKTASTPEDEKKKAEEAAKKKKAEEDKKKTDEE
CCCHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHH
KKKEEEAKKKAEEEAKKKAEEEAKNLTAPAGLRASYNAGSKQINVSWSAVDGATYEVTVN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEEC
GSTTTVSSTSVSVSGGNPGDTVSINVVAVKDGNRSPASSTTVKIPDS
CCEEEEECCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCC
>Mature Secondary Structure 
ADKPQTRSQYRNKQSGGSKKKSQKRGKRVVANIFKTIFFVGLFLAFFGIAAGATVFYDY
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEHH
AKDAPKLTDSKLRDPLSSKLLDKDGKVFAEVGTERREYIEYKDIPETLKNAILTTEDARF
HCCCCCCCHHHHHCHHHHHHHCCCCCCHHHHCCHHHHHHCCCCHHHHHHHHEECCCCCCH
YEHDGIDPIRLGGAVIANLTDGFGAEGASTLSQQIIKMSYLDYTNKTLARKAQEAWLALQ
HHCCCCCEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
LEEKYSKNDILEIYVNKVYMSDRVHGMQTASEHYFGKNLKDLTLAQTALLAGMPQSPNNY
EHHHCCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
NPYEHPEAAKKRRDQVLTNMYTHDKITKEEMTEAQKTSITTGLRSKKDREDKIYKYDSYV
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TQVLSEIPKEYDVYRDGLTIHTALDRDAQEYTEKMLNTNEIVNFTDDEMQAGIVLQDTKT
HHHHHHCCHHHHHHHCCEEEEEHHCCCHHHHHHHHCCCCCEEECCCCHHHCCEEEEECCC
GRVQAIGGGRKQKVTRGYNYATQVKRSVGSTMKPIADYGPAFEYLDWSTAHILEDEPYTY
CEEEEECCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCHHHCCCCHHEEECCCCEEE
SGGTPINNWDFAYKGPISVRQALYQSRNIPALKTLQAVGLDKSEEFVNKLGITYDEGQNV
CCCCCCCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCEECCCCCC
ESNAIGANSSNPMQMAGAYAAFGNKGIYNKPHTVTKIVLSDGQTEIDTEPQSTVAMKEST
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHEEHHHHH
AYMVSDVLKDVLSIGTGTSAAVPGVPAAGKTGTTNIPPEFTSKYYYPSGAARDSWFAGYT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCEECEE
TNYSIAVWTGYDDKKKYVSASEQKIAQRMFSKLMAHASAGKTTADFKMPSNVVSVPILKG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
SNPIARAAAGTASDKVSYELFLSGTAPTKTASTPEDEKKKAEEAAKKKKAEEDKKKTDEE
CCCHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHH
KKKEEEAKKKAEEEAKKKAEEEAKNLTAPAGLRASYNAGSKQINVSWSAVDGATYEVTVN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEEC
GSTTTVSSTSVSVSGGNPGDTVSINVVAVKDGNRSPASSTTVKIPDS
CCEEEEECCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7814321; 8760912; 9384377; 9721295; 10322023 [H]