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The map label for this gene is ppdK [H]

Identifier: 226224470

GI number: 226224470

Start: 1926254

End: 1928893

Strand: Direct

Name: ppdK [H]

Synonym: Lm4b_01883

Alternate gene names: 226224470

Gene position: 1926254-1928893 (Clockwise)

Preceding gene: 226224469

Following gene: 226224482

Centisome position: 66.13

GC content: 42.46

Gene sequence:

>2640_bases
GTGAGAAAATTTGTCTATCAGTTCAGTGAAGGTTCTAAAGAAATGAAAAATATTTTAGGAGGAAAAGGTGCTAATTTGGC
CGAAATGACGAATATCGGTTTGCCTGTGCCTCCTGGTTTTATTATCTCGACCGATGCCTGTAACGATTACACGGCAAACA
ATAAACACCTTTCCGAAGCTATATTTGAAGAAGTAAAAATTCATTTAGCAGAACTGGAAAAACAAACCGGGAAAATTTTC
GGATTTGCAGAAAACCCTCTACTCGTTTCCGTTCGTTCGGGTGCCCCTTTTTCGATGCCCGGTATGATGGATACCGTGCT
CAATCTCGGTTTAAATGACCAAGCCGCAGAAGGGCTCGCCAACTTAACTGGTGATGCTCGTTCGGCTTATGATTCATACC
GCCGTTTTATTCAAATGTTCGGTGACGTTGTTTTTGAAATTCCAAGCTACCAATTTGAACAGGCTCTTAGTCGCATCAAA
AAAGCAAACAACTACCGCTTAGATACAGAATTAACTGCGACTGATTTAAGCAATCTCATCGATGCTTACAAACTAATTTT
TAGCCAAGCAACTGGTATAGACTTTCCACAAGATCCACTGGAACAACTGCGGCTTGCGATAATCGCTGTTTTTGATTCCT
GGATGAATCCTCGCGCTGTTATTTACCGCAGATTACATGATATTAATGCAAGTTTTGGTACAGCCGTTAATATTCAAGCG
ATGGTTTTTGGAAACACTGGAGAAACGAGTGGAACTGGCATTACCTTTACCCGCAATCCTTCCACCGGTGAAAAGAAGGT
GTTCGGGGAATTTTTACTGAATGCGCAAGGTGAAGATGTGGTTGCAGGAATTCGCACCCCTGAGCCGATAAGTGCGCTGG
AAGAAAGAATGCCCACTGTTTACAAGGAACTCCTCCAGACTTGTGAACTTCTCGAAAATCATTATTTAGACATGCAAGAT
ATTGAATTTACCATCGAAAAAGGCAAACTCTATGTCTTACAAACTAGAAGTGGCAAGCGAACAGCCAAAGCAGCTATTCA
AGCGGCCATTGATTTTGTCCATGAAGGAAAAATCACTCGTCAAGAAGCCATTATGCGTGTGGAAACAAAACAACTAAACC
AATTACTTCATCCAACTTTTCTTGAAAGCGCTCTTAAAAATGGTCAAGTCATCGCAACTGGTTTACCTGCAAGCCCTGGT
GCTGCAACGGGGCAAATTTTCTTTGAAGCAAAAGAAGCTGTCCAAGCAGCCGAACGTGGCATTCCTGTCATTTTAGTTCG
TAATGAGACTTCTCCTGAGGATATCGAAGGCATGGCACGGAGTAACGCTATTTTAACCGCTCATGGTGGTATGACTTCTC
ACGCTGCCGTCGTTGCCCGCGGAATGGGAAAATGCTGTATCGCTGGATGTGCCGAACTTACTATTAATGAAAAAGATAAA
ACGATCATTCTTCCTACTGGCGACACACTGCATGAAGGTGATGCGCTCTCCCTTGATGGTAGTTCCGGCAAAGTTTACCT
TGGAGAAATTGCCCTCAGAGAAGCGAAAATTGGTGGTCATTTTGACGAGCTGATGGCCTGGGCTGATGCGGAGAAAAGAT
TAATGATTCGAGTAAATGCAGATACCCCAGCAGACTTTAAAAAAGCGCTTTTATTTGGTGCGGAAGGTATTGGTCTTTGC
CGTACCGAGCATATGTTTTTTGATGAAAAACGAATTCCTTATGTACGCCAAATGATTTTAGCAGAATCATTAAACGAGCG
CGAATCTGTTTTAGCAATTTTAAAAGAAATGCAAAAAGCCGATTTCAATGAATTATTCCGTATTGCAGACGGTCGTTCGG
TAAACATTCGCTTGCTTGATCCACCTTTACATGAATTTTTACCAACAATGAAGCAAGAAATAGAACAATTGGCTGGCGCG
ATGAATCGAACCGTTCCTCAAATTACAAACCGAATCAACGGACTAAGAGAAGCCAATCCCATGCTCGGACATCGTGGTTG
CCGGCTTGCTATTACGTTCCCAGAAATTTACCGGATGCAGGCGGAAGCAATTATCGAAAGTGCAATTATCGTTCATAATG
AAGGAATAGATGTTCACCCGGAAATCATGATTCCACTCATCGCTACGAAAAACGAACTTAGCTATATAAAAAAAGAACTG
CAACAAGCAGTCCAAGCTATTTTTGATAGAGAACAAGTGGTGATTCCCTATGATATCGGAACAATGATTGAAATTCCGCG
AGCGTGTGTAACTGCAGACGAAATCGCCGAGGAAGCAGCGTTCTTTAGCTTTGGAACCAATGATTTAACACAGCTCACTT
ATGGTTTTTCACGTGATGATGCGACCAAGTTCCTAGCCGATTATTATGAAAAAGATATTTTACCTAAAGATCCATTTGTT
TCGATTGATAAAAATGGTGTCGGCGCACTAGTTGAAATGGCAGTTACACGTGGACGAATGACTTCGGAACATTTAAAAAT
GGGGGTTTGCGGCGAACATGGTGGCGATCCGGAATCTATCCAGTTCTTCCATCAATTAGGACTTACCTATGTTTCTTGTT
CCCCGTACCGCGTTCCCATTGCCCGCCTTGCAGCAGCTCAAGCAGCATTAGCTGAGAAACAATTAGTTCCAACTATCTAA

Upstream 100 bases:

>100_bases
CTGCCAAGTCATTCATGTAGAAGATAAAGCCATTGAGGAAACCGCTGCCCTAATCACGGAAATTATCACGAGTTACCACT
AATTTGAAGAGGTGAATAAT

Downstream 100 bases:

>100_bases
AAAAAGCCAGATGACAATCAAATAGACTGTCATCTGGCTTTTTCTCCTATTATTTTTCACAAATCGCAAAGTAATTTCCT
TCGTTATCCGAAAAATTGAA

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 879; Mature: 879

Protein sequence:

>879_residues
MRKFVYQFSEGSKEMKNILGGKGANLAEMTNIGLPVPPGFIISTDACNDYTANNKHLSEAIFEEVKIHLAELEKQTGKIF
GFAENPLLVSVRSGAPFSMPGMMDTVLNLGLNDQAAEGLANLTGDARSAYDSYRRFIQMFGDVVFEIPSYQFEQALSRIK
KANNYRLDTELTATDLSNLIDAYKLIFSQATGIDFPQDPLEQLRLAIIAVFDSWMNPRAVIYRRLHDINASFGTAVNIQA
MVFGNTGETSGTGITFTRNPSTGEKKVFGEFLLNAQGEDVVAGIRTPEPISALEERMPTVYKELLQTCELLENHYLDMQD
IEFTIEKGKLYVLQTRSGKRTAKAAIQAAIDFVHEGKITRQEAIMRVETKQLNQLLHPTFLESALKNGQVIATGLPASPG
AATGQIFFEAKEAVQAAERGIPVILVRNETSPEDIEGMARSNAILTAHGGMTSHAAVVARGMGKCCIAGCAELTINEKDK
TIILPTGDTLHEGDALSLDGSSGKVYLGEIALREAKIGGHFDELMAWADAEKRLMIRVNADTPADFKKALLFGAEGIGLC
RTEHMFFDEKRIPYVRQMILAESLNERESVLAILKEMQKADFNELFRIADGRSVNIRLLDPPLHEFLPTMKQEIEQLAGA
MNRTVPQITNRINGLREANPMLGHRGCRLAITFPEIYRMQAEAIIESAIIVHNEGIDVHPEIMIPLIATKNELSYIKKEL
QQAVQAIFDREQVVIPYDIGTMIEIPRACVTADEIAEEAAFFSFGTNDLTQLTYGFSRDDATKFLADYYEKDILPKDPFV
SIDKNGVGALVEMAVTRGRMTSEHLKMGVCGEHGGDPESIQFFHQLGLTYVSCSPYRVPIARLAAAQAALAEKQLVPTI

Sequences:

>Translated_879_residues
MRKFVYQFSEGSKEMKNILGGKGANLAEMTNIGLPVPPGFIISTDACNDYTANNKHLSEAIFEEVKIHLAELEKQTGKIF
GFAENPLLVSVRSGAPFSMPGMMDTVLNLGLNDQAAEGLANLTGDARSAYDSYRRFIQMFGDVVFEIPSYQFEQALSRIK
KANNYRLDTELTATDLSNLIDAYKLIFSQATGIDFPQDPLEQLRLAIIAVFDSWMNPRAVIYRRLHDINASFGTAVNIQA
MVFGNTGETSGTGITFTRNPSTGEKKVFGEFLLNAQGEDVVAGIRTPEPISALEERMPTVYKELLQTCELLENHYLDMQD
IEFTIEKGKLYVLQTRSGKRTAKAAIQAAIDFVHEGKITRQEAIMRVETKQLNQLLHPTFLESALKNGQVIATGLPASPG
AATGQIFFEAKEAVQAAERGIPVILVRNETSPEDIEGMARSNAILTAHGGMTSHAAVVARGMGKCCIAGCAELTINEKDK
TIILPTGDTLHEGDALSLDGSSGKVYLGEIALREAKIGGHFDELMAWADAEKRLMIRVNADTPADFKKALLFGAEGIGLC
RTEHMFFDEKRIPYVRQMILAESLNERESVLAILKEMQKADFNELFRIADGRSVNIRLLDPPLHEFLPTMKQEIEQLAGA
MNRTVPQITNRINGLREANPMLGHRGCRLAITFPEIYRMQAEAIIESAIIVHNEGIDVHPEIMIPLIATKNELSYIKKEL
QQAVQAIFDREQVVIPYDIGTMIEIPRACVTADEIAEEAAFFSFGTNDLTQLTYGFSRDDATKFLADYYEKDILPKDPFV
SIDKNGVGALVEMAVTRGRMTSEHLKMGVCGEHGGDPESIQFFHQLGLTYVSCSPYRVPIARLAAAQAALAEKQLVPTI
>Mature_879_residues
MRKFVYQFSEGSKEMKNILGGKGANLAEMTNIGLPVPPGFIISTDACNDYTANNKHLSEAIFEEVKIHLAELEKQTGKIF
GFAENPLLVSVRSGAPFSMPGMMDTVLNLGLNDQAAEGLANLTGDARSAYDSYRRFIQMFGDVVFEIPSYQFEQALSRIK
KANNYRLDTELTATDLSNLIDAYKLIFSQATGIDFPQDPLEQLRLAIIAVFDSWMNPRAVIYRRLHDINASFGTAVNIQA
MVFGNTGETSGTGITFTRNPSTGEKKVFGEFLLNAQGEDVVAGIRTPEPISALEERMPTVYKELLQTCELLENHYLDMQD
IEFTIEKGKLYVLQTRSGKRTAKAAIQAAIDFVHEGKITRQEAIMRVETKQLNQLLHPTFLESALKNGQVIATGLPASPG
AATGQIFFEAKEAVQAAERGIPVILVRNETSPEDIEGMARSNAILTAHGGMTSHAAVVARGMGKCCIAGCAELTINEKDK
TIILPTGDTLHEGDALSLDGSSGKVYLGEIALREAKIGGHFDELMAWADAEKRLMIRVNADTPADFKKALLFGAEGIGLC
RTEHMFFDEKRIPYVRQMILAESLNERESVLAILKEMQKADFNELFRIADGRSVNIRLLDPPLHEFLPTMKQEIEQLAGA
MNRTVPQITNRINGLREANPMLGHRGCRLAITFPEIYRMQAEAIIESAIIVHNEGIDVHPEIMIPLIATKNELSYIKKEL
QQAVQAIFDREQVVIPYDIGTMIEIPRACVTADEIAEEAAFFSFGTNDLTQLTYGFSRDDATKFLADYYEKDILPKDPFV
SIDKNGVGALVEMAVTRGRMTSEHLKMGVCGEHGGDPESIQFFHQLGLTYVSCSPYRVPIARLAAAQAALAEKQLVPTI

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=452, Percent_Identity=26.3274336283186, Blast_Score=113, Evalue=6e-26,
Organism=Escherichia coli, GI1787994, Length=905, Percent_Identity=22.9834254143646, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI48994992, Length=379, Percent_Identity=25.065963060686, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1788726, Length=461, Percent_Identity=22.7765726681128, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1789193, Length=437, Percent_Identity=22.883295194508, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97051; Mature: 97051

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFVYQFSEGSKEMKNILGGKGANLAEMTNIGLPVPPGFIISTDACNDYTANNKHLSEA
CCCEEEEHHCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
IFEEVKIHLAELEKQTGKIFGFAENPLLVSVRSGAPFSMPGMMDTVLNLGLNDQAAEGLA
HHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHH
NLTGDARSAYDSYRRFIQMFGDVVFEIPSYQFEQALSRIKKANNYRLDTELTATDLSNLI
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCEEHHHHHHHHH
DAYKLIFSQATGIDFPQDPLEQLRLAIIAVFDSWMNPRAVIYRRLHDINASFGTAVNIQA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEE
MVFGNTGETSGTGITFTRNPSTGEKKVFGEFLLNAQGEDVVAGIRTPEPISALEERMPTV
EEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHH
YKELLQTCELLENHYLDMQDIEFTIEKGKLYVLQTRSGKRTAKAAIQAAIDFVHEGKITR
HHHHHHHHHHHHHHCCCHHHCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCH
QEAIMRVETKQLNQLLHPTFLESALKNGQVIATGLPASPGAATGQIFFEAKEAVQAAERG
HHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IPVILVRNETSPEDIEGMARSNAILTAHGGMTSHAAVVARGMGKCCIAGCAELTINEKDK
CCEEEEECCCCCHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCHHHHHCCHHEEECCCCC
TIILPTGDTLHEGDALSLDGSSGKVYLGEIALREAKIGGHFDELMAWADAEKRLMIRVNA
EEEEECCCCCCCCCEEEECCCCCCEEEEHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECC
DTPADFKKALLFGAEGIGLCRTEHMFFDEKRIPYVRQMILAESLNERESVLAILKEMQKA
CCCHHHHHHHHHCCCCCCCEEEHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHC
DFNELFRIADGRSVNIRLLDPPLHEFLPTMKQEIEQLAGAMNRTVPQITNRINGLREANP
CHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
MLGHRGCRLAITFPEIYRMQAEAIIESAIIVHNEGIDVHPEIMIPLIATKNELSYIKKEL
CCCCCCCEEEEECHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEEECCHHHHHHHHHH
QQAVQAIFDREQVVIPYDIGTMIEIPRACVTADEIAEEAAFFSFGTNDLTQLTYGFSRDD
HHHHHHHHCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCH
ATKFLADYYEKDILPKDPFVSIDKNGVGALVEMAVTRGRMTSEHLKMGVCGEHGGDPESI
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHH
QFFHQLGLTYVSCSPYRVPIARLAAAQAALAEKQLVPTI
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRKFVYQFSEGSKEMKNILGGKGANLAEMTNIGLPVPPGFIISTDACNDYTANNKHLSEA
CCCEEEEHHCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
IFEEVKIHLAELEKQTGKIFGFAENPLLVSVRSGAPFSMPGMMDTVLNLGLNDQAAEGLA
HHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHH
NLTGDARSAYDSYRRFIQMFGDVVFEIPSYQFEQALSRIKKANNYRLDTELTATDLSNLI
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCEEHHHHHHHHH
DAYKLIFSQATGIDFPQDPLEQLRLAIIAVFDSWMNPRAVIYRRLHDINASFGTAVNIQA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEE
MVFGNTGETSGTGITFTRNPSTGEKKVFGEFLLNAQGEDVVAGIRTPEPISALEERMPTV
EEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHH
YKELLQTCELLENHYLDMQDIEFTIEKGKLYVLQTRSGKRTAKAAIQAAIDFVHEGKITR
HHHHHHHHHHHHHHCCCHHHCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCH
QEAIMRVETKQLNQLLHPTFLESALKNGQVIATGLPASPGAATGQIFFEAKEAVQAAERG
HHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IPVILVRNETSPEDIEGMARSNAILTAHGGMTSHAAVVARGMGKCCIAGCAELTINEKDK
CCEEEEECCCCCHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCHHHHHCCHHEEECCCCC
TIILPTGDTLHEGDALSLDGSSGKVYLGEIALREAKIGGHFDELMAWADAEKRLMIRVNA
EEEEECCCCCCCCCEEEECCCCCCEEEEHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECC
DTPADFKKALLFGAEGIGLCRTEHMFFDEKRIPYVRQMILAESLNERESVLAILKEMQKA
CCCHHHHHHHHHCCCCCCCEEEHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHC
DFNELFRIADGRSVNIRLLDPPLHEFLPTMKQEIEQLAGAMNRTVPQITNRINGLREANP
CHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
MLGHRGCRLAITFPEIYRMQAEAIIESAIIVHNEGIDVHPEIMIPLIATKNELSYIKKEL
CCCCCCCEEEEECHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEEECCHHHHHHHHHH
QQAVQAIFDREQVVIPYDIGTMIEIPRACVTADEIAEEAAFFSFGTNDLTQLTYGFSRDD
HHHHHHHHCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCH
ATKFLADYYEKDILPKDPFVSIDKNGVGALVEMAVTRGRMTSEHLKMGVCGEHGGDPESI
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHH
QFFHQLGLTYVSCSPYRVPIARLAAAQAALAEKQLVPTI
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]