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The map label for this gene is norM [H]

Identifier: 226224449

GI number: 226224449

Start: 1908694

End: 1910064

Strand: Direct

Name: norM [H]

Synonym: Lm4b_01862

Alternate gene names: 226224449

Gene position: 1908694-1910064 (Clockwise)

Preceding gene: 226224445

Following gene: 226224467

Centisome position: 65.53

GC content: 40.48

Gene sequence:

>1371_bases
TTGCAACAAACAGTCACATATGGGGCAAAATGGAAACAATTTTTAACGATATTCACACCGATTGTCATTACGCAACTCAC
GCTTTTCTCTATGACATTTTTCGATACGACGATGAGCGGAAATTATTCAAATCAGGCACTGGCTGGTGTAGCAATTGGGA
GTTCGTTCTGGGCTCCAGTAAATGCTGCTTTTTCTGGGTTACTGATGGCCATCACGCCAATCATCGCCCAATTAATCGGC
GCAAAGAAAGAAAAACAAGTCAAAAACACGGTGCATAACGGTCTATATATTGCTTTATTTTTAGCTTTTATTTTAATTCT
TATTAATTTTTTGGTCGTTCCAACGATTTTGACACACATGCCCGTTACGGCAGAAGTCGCGGGGATTGCTCGTCATTTCT
TAAATGGCATTTGTATCGGAATCCCGGCTTTCTTTATTTCCGCAATTTTGCGTTCTTTTATTGATTCGCTTGGTTTAACT
AGGGTAACGATGCTCATTACACTTTGTACCGTTCCATTTAATATCTTTTTAAATTACTGCTTTATTTTTGGAAACTTTGG
TTTTCCGGAAATGGGTGGAGCTGGTAGTGGTTACGCGACAGGGATTACGTATTGGCTCGTTGTTTTAGTTAGTGTTATTT
TGATTCAAACACAAACTAGATTACGAAAATTTGGTATTTTCAAAGGGCTTACGGCACTTCGTTTTTCTAAGATAAAAGAG
ATCATCGGCATTGGCGTTCCAAATGGTTTGACAATTTTATTTGAAACGAGTATTTTCTCAGCGGTAACCATTTTAATGGG
AGCCTTTGGGACGGAGACAATTGCGGCACATCAATCCGCAAATAGTGTCTGCACACTGCTCTATGCTTTCCCGCTTAGTG
TTGCTTCAACGTTGACCATTCTTGGCGGGTACGAGACAGGGGCAAAACGCTTAAAAGATGCCAAACAGTATCGTCATATT
GGTATGGCTGCTGCGATTTTCATTGGTTGTGTAAATGGTGCAATCCTATTTTTCTTCCGCGATATTATTGCTGGTTTTTA
TACGAACGATCCCGCTTTGAGCAATTTAATCATGCATTTCTTAGTTTACGCGATATTGTTTCAATTTGCGGATGCTGTCC
TGTCGCCAGTTCTCGGGGCACTTAGAGGATATAAAGATGTTACGGTCACCTCGATTGTTGCCTTTATTTCTTATTGGTTG
ATTGGGCTTCCAGTAGGCTACGGTTTATCGTTTACGAATCTTGGCCCATTCGGTTACTGGATAGGGTTAAGTACTGGCCT
GTTTGTCGCCGCATTTATTTTATCCATTCGGGTTCGTAAAACGGAGCAAAAACTTTCTTTCAACACAAAAGACGCAGAGA
TTTCGAGTTAA

Upstream 100 bases:

>100_bases
ACAAAGATAGTACATATACGAAAGGAAAAGTTTTTTGTTACGCCGCCAGAACGCCTTATTTATGCTATAATCTGAATGGA
TGCAAAAAGGAGATGATTAC

Downstream 100 bases:

>100_bases
TCTCTGCGTCTTTTTTTATTTGGCTAAACCATCATGAATTTTATCTAAATTGTATTGCATCATTTCTAAATACGTGTCGC
CCACTTCACCTTTTTTAGCA

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MQQTVTYGAKWKQFLTIFTPIVITQLTLFSMTFFDTTMSGNYSNQALAGVAIGSSFWAPVNAAFSGLLMAITPIIAQLIG
AKKEKQVKNTVHNGLYIALFLAFILILINFLVVPTILTHMPVTAEVAGIARHFLNGICIGIPAFFISAILRSFIDSLGLT
RVTMLITLCTVPFNIFLNYCFIFGNFGFPEMGGAGSGYATGITYWLVVLVSVILIQTQTRLRKFGIFKGLTALRFSKIKE
IIGIGVPNGLTILFETSIFSAVTILMGAFGTETIAAHQSANSVCTLLYAFPLSVASTLTILGGYETGAKRLKDAKQYRHI
GMAAAIFIGCVNGAILFFFRDIIAGFYTNDPALSNLIMHFLVYAILFQFADAVLSPVLGALRGYKDVTVTSIVAFISYWL
IGLPVGYGLSFTNLGPFGYWIGLSTGLFVAAFILSIRVRKTEQKLSFNTKDAEISS

Sequences:

>Translated_456_residues
MQQTVTYGAKWKQFLTIFTPIVITQLTLFSMTFFDTTMSGNYSNQALAGVAIGSSFWAPVNAAFSGLLMAITPIIAQLIG
AKKEKQVKNTVHNGLYIALFLAFILILINFLVVPTILTHMPVTAEVAGIARHFLNGICIGIPAFFISAILRSFIDSLGLT
RVTMLITLCTVPFNIFLNYCFIFGNFGFPEMGGAGSGYATGITYWLVVLVSVILIQTQTRLRKFGIFKGLTALRFSKIKE
IIGIGVPNGLTILFETSIFSAVTILMGAFGTETIAAHQSANSVCTLLYAFPLSVASTLTILGGYETGAKRLKDAKQYRHI
GMAAAIFIGCVNGAILFFFRDIIAGFYTNDPALSNLIMHFLVYAILFQFADAVLSPVLGALRGYKDVTVTSIVAFISYWL
IGLPVGYGLSFTNLGPFGYWIGLSTGLFVAAFILSIRVRKTEQKLSFNTKDAEISS
>Mature_456_residues
MQQTVTYGAKWKQFLTIFTPIVITQLTLFSMTFFDTTMSGNYSNQALAGVAIGSSFWAPVNAAFSGLLMAITPIIAQLIG
AKKEKQVKNTVHNGLYIALFLAFILILINFLVVPTILTHMPVTAEVAGIARHFLNGICIGIPAFFISAILRSFIDSLGLT
RVTMLITLCTVPFNIFLNYCFIFGNFGFPEMGGAGSGYATGITYWLVVLVSVILIQTQTRLRKFGIFKGLTALRFSKIKE
IIGIGVPNGLTILFETSIFSAVTILMGAFGTETIAAHQSANSVCTLLYAFPLSVASTLTILGGYETGAKRLKDAKQYRHI
GMAAAIFIGCVNGAILFFFRDIIAGFYTNDPALSNLIMHFLVYAILFQFADAVLSPVLGALRGYKDVTVTSIVAFISYWL
IGLPVGYGLSFTNLGPFGYWIGLSTGLFVAAFILSIRVRKTEQKLSFNTKDAEISS

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=415, Percent_Identity=21.9277108433735, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI48994890, Length=438, Percent_Identity=31.5068493150685, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI226510954, Length=441, Percent_Identity=23.5827664399093, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6320545, Length=434, Percent_Identity=23.0414746543779, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321821, Length=374, Percent_Identity=22.7272727272727, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49589; Mature: 49589

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]