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The map label for this gene is gsaB

Identifier: 226224288

GI number: 226224288

Start: 1736287

End: 1737585

Strand: Direct

Name: gsaB

Synonym: Lm4b_01699

Alternate gene names: 226224288

Gene position: 1736287-1737585 (Clockwise)

Preceding gene: 226224280

Following gene: 226224289

Centisome position: 59.61

GC content: 42.73

Gene sequence:

>1299_bases
ATGGATCATTCTATGTCAAAAAAATTGCATGATGAAGCACTTTTACATATAGTTGGCGGGGTTAATAGCCCATCTAGGTC
AAATAAAGGGGTTGGCGGCGGAATTCCTGTAACAATGGAACGGGCTAGCGGCGCTTATTTTTATGATGTGGATGGGAATA
AGTATATTGACTACTTAGCTGCGTTTGGACCAATCATTACTGGACATGCACATCCTCATATTACAGAAGCAATTACAAAA
GCTGCGCAAAATGGTGTTTTGTACGGAACACCTACTAAACACGAAATCACTTTTGCGAAAATGTTAAAAGAGGCCATTCC
TTCACTTGAGAAGGTCCGTTTTACAAATTCCGGGACGGAAGCTGTTATGACAACGATTCGTGTCGCTCGCGCTTATACAG
GCAGAGATAAAATCATTAAATTCGCTGGGTGTTACCACGGCCACTTTGATTTAGTACTCGTGGAAGCTGGTTCTGGACCT
TCTACGCTTGGCATTCCAGACTCCGCTGGGGTAACGAAATCAACCGCAGAAGAAGTTATTACTGTACCTTTTAATGACCT
TGCATCGTTTAAAGAGGCATTAGCTGTTTGGAGCGATCAAGTTGCTGCTGTTTTAGTAGAACCTATTGTTGGGAATTTTG
GAATGGTTGCGCCAGAAGATGGTTTTTTAGAAGCTGTTAATGAACTCGCACATGCCAATGGTTCTTTAGTAATTTATGAT
GAAGTTATTACGGCTTTCCGTTTTATGTACGGCGGCGCGCAGAACTATTTAGGTGTTATTCCAGATTTGACTGCTATGGG
TAAAATTATTGGTGGCGGGCTTCCGATTGGTGCTTACGGTGGTCGGATTGATATCATGGAAAAAGTAGCACCACTCGGAC
CGGCTTATCAGGCTGGGACGCATGCGGGGAATCCGGCATCTATTCTTTCAGGAATTGCTTGTCTCGAAGTTTTACAAGAA
GAAGGACTGTATGAGCGCTTTGAAAAATATGGCTCGATGCTGAAAGATGGCATTGAAAAAGCCGCGCTAAAACACGGCAT
TGCTGTTACGGTTAACCAAATTGTCGGTGCTCTAACTGTTTACTTTACGGAAGATCCTGTGACTAATTATGCTGAGGCTG
GCGCTACAAATGGCGAGTTATTCGGTCGTTTCTTTAAAGGAATGCTAGAGGAAGGCATTAATTTGGCGCCTTCTAAATAT
GAAGCATGGTTCATCACTTCCGCACACTCGGAAGCTGATATTTTAGAAACAATCCAAGCAGTTGATACTGTTTTTGGAAA
AATGGTTCAAGATAACTAG

Upstream 100 bases:

>100_bases
TTTTTTTCATTTAACGGCAATTAAATTGTTTTTTCGATTGCGGCATCTTATTTTTTCGCATAGAATATAAGAGGTATTAA
AATTTTGAGGAGATGACGAA

Downstream 100 bases:

>100_bases
GTAATATTACTTTATAAACTGGTGAGAAACGTTATAATAGAAGAAGAATGAGCGGGAAAACTTAAAAATCCCGCTCAAAT
AGTCTATAGAAAGGAATAAC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1 [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MDHSMSKKLHDEALLHIVGGVNSPSRSNKGVGGGIPVTMERASGAYFYDVDGNKYIDYLAAFGPIITGHAHPHITEAITK
AAQNGVLYGTPTKHEITFAKMLKEAIPSLEKVRFTNSGTEAVMTTIRVARAYTGRDKIIKFAGCYHGHFDLVLVEAGSGP
STLGIPDSAGVTKSTAEEVITVPFNDLASFKEALAVWSDQVAAVLVEPIVGNFGMVAPEDGFLEAVNELAHANGSLVIYD
EVITAFRFMYGGAQNYLGVIPDLTAMGKIIGGGLPIGAYGGRIDIMEKVAPLGPAYQAGTHAGNPASILSGIACLEVLQE
EGLYERFEKYGSMLKDGIEKAALKHGIAVTVNQIVGALTVYFTEDPVTNYAEAGATNGELFGRFFKGMLEEGINLAPSKY
EAWFITSAHSEADILETIQAVDTVFGKMVQDN

Sequences:

>Translated_432_residues
MDHSMSKKLHDEALLHIVGGVNSPSRSNKGVGGGIPVTMERASGAYFYDVDGNKYIDYLAAFGPIITGHAHPHITEAITK
AAQNGVLYGTPTKHEITFAKMLKEAIPSLEKVRFTNSGTEAVMTTIRVARAYTGRDKIIKFAGCYHGHFDLVLVEAGSGP
STLGIPDSAGVTKSTAEEVITVPFNDLASFKEALAVWSDQVAAVLVEPIVGNFGMVAPEDGFLEAVNELAHANGSLVIYD
EVITAFRFMYGGAQNYLGVIPDLTAMGKIIGGGLPIGAYGGRIDIMEKVAPLGPAYQAGTHAGNPASILSGIACLEVLQE
EGLYERFEKYGSMLKDGIEKAALKHGIAVTVNQIVGALTVYFTEDPVTNYAEAGATNGELFGRFFKGMLEEGINLAPSKY
EAWFITSAHSEADILETIQAVDTVFGKMVQDN
>Mature_432_residues
MDHSMSKKLHDEALLHIVGGVNSPSRSNKGVGGGIPVTMERASGAYFYDVDGNKYIDYLAAFGPIITGHAHPHITEAITK
AAQNGVLYGTPTKHEITFAKMLKEAIPSLEKVRFTNSGTEAVMTTIRVARAYTGRDKIIKFAGCYHGHFDLVLVEAGSGP
STLGIPDSAGVTKSTAEEVITVPFNDLASFKEALAVWSDQVAAVLVEPIVGNFGMVAPEDGFLEAVNELAHANGSLVIYD
EVITAFRFMYGGAQNYLGVIPDLTAMGKIIGGGLPIGAYGGRIDIMEKVAPLGPAYQAGTHAGNPASILSGIACLEVLQE
EGLYERFEKYGSMLKDGIEKAALKHGIAVTVNQIVGALTVYFTEDPVTNYAEAGATNGELFGRFFKGMLEEGINLAPSKY
EAWFITSAHSEADILETIQAVDTVFGKMVQDN

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=351, Percent_Identity=31.3390313390313, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI13994255, Length=337, Percent_Identity=28.1899109792285, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI37574042, Length=339, Percent_Identity=26.8436578171091, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI24119277, Length=340, Percent_Identity=27.9411764705882, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI226442705, Length=340, Percent_Identity=26.7647058823529, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI284507298, Length=260, Percent_Identity=31.9230769230769, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI226442709, Length=307, Percent_Identity=27.0358306188925, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=47.887323943662, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1789016, Length=380, Percent_Identity=28.9473684210526, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787560, Length=335, Percent_Identity=30.1492537313433, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI145693181, Length=372, Percent_Identity=28.7634408602151, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789759, Length=324, Percent_Identity=29.320987654321, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788044, Length=287, Percent_Identity=31.3588850174216, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786991, Length=383, Percent_Identity=23.7597911227154, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144271, Length=317, Percent_Identity=28.391167192429, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71992977, Length=344, Percent_Identity=27.906976744186, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32564660, Length=330, Percent_Identity=29.0909090909091, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI25144274, Length=156, Percent_Identity=35.2564102564103, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=322, Percent_Identity=29.5031055900621, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=341, Percent_Identity=22.8739002932551, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21357415, Length=315, Percent_Identity=31.1111111111111, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161085790, Length=344, Percent_Identity=29.0697674418605, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28574759, Length=344, Percent_Identity=29.0697674418605, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46031; Mature: 46031

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHSMSKKLHDEALLHIVGGVNSPSRSNKGVGGGIPVTMERASGAYFYDVDGNKYIDYLA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHH
AFGPIITGHAHPHITEAITKAAQNGVLYGTPTKHEITFAKMLKEAIPSLEKVRFTNSGTE
HHCCHHCCCCCCHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCHHHHEEECCCCHH
AVMTTIRVARAYTGRDKIIKFAGCYHGHFDLVLVEAGSGPSTLGIPDSAGVTKSTAEEVI
HHHHHHHHHHHHCCHHHHEEEECEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHCEE
TVPFNDLASFKEALAVWSDQVAAVLVEPIVGNFGMVAPEDGFLEAVNELAHANGSLVIYD
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEHH
EVITAFRFMYGGAQNYLGVIPDLTAMGKIIGGGLPIGAYGGRIDIMEKVAPLGPAYQAGT
HHHHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCHHCCC
HAGNPASILSGIACLEVLQEEGLYERFEKYGSMLKDGIEKAALKHGIAVTVNQIVGALTV
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHEEE
YFTEDPVTNYAEAGATNGELFGRFFKGMLEEGINLAPSKYEAWFITSAHSEADILETIQA
EEECCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHH
VDTVFGKMVQDN
HHHHHHHHHCCC
>Mature Secondary Structure
MDHSMSKKLHDEALLHIVGGVNSPSRSNKGVGGGIPVTMERASGAYFYDVDGNKYIDYLA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHH
AFGPIITGHAHPHITEAITKAAQNGVLYGTPTKHEITFAKMLKEAIPSLEKVRFTNSGTE
HHCCHHCCCCCCHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCHHHHEEECCCCHH
AVMTTIRVARAYTGRDKIIKFAGCYHGHFDLVLVEAGSGPSTLGIPDSAGVTKSTAEEVI
HHHHHHHHHHHHCCHHHHEEEECEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHCEE
TVPFNDLASFKEALAVWSDQVAAVLVEPIVGNFGMVAPEDGFLEAVNELAHANGSLVIYD
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEHH
EVITAFRFMYGGAQNYLGVIPDLTAMGKIIGGGLPIGAYGGRIDIMEKVAPLGPAYQAGT
HHHHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCHHCCC
HAGNPASILSGIACLEVLQEEGLYERFEKYGSMLKDGIEKAALKHGIAVTVNQIVGALTV
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHEEE
YFTEDPVTNYAEAGATNGELFGRFFKGMLEEGINLAPSKYEAWFITSAHSEADILETIQA
EEECCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHH
VDTVFGKMVQDN
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA