Click here to switch to the map view.

The map label for this gene is yiaE [C]

Identifier: 226224287

GI number: 226224287

Start: 1735201

End: 1736142

Strand: Reverse

Name: yiaE [C]

Synonym: Lm4b_01698

Alternate gene names: 226224287

Gene position: 1736142-1735201 (Counterclockwise)

Preceding gene: 226224290

Following gene: 226224286

Centisome position: 59.61

GC content: 34.39

Gene sequence:

>942_bases
ATGAACATTTTATTTACATTAGATGTCCCAGAACATTTACAGACTTTGCAAGCAGAGAAATTTCCAGAGGATACTTTTTA
TTATGAGAGCAATGATCATTTTGCCAATTTAGCGGAGATAGATATCATCGTCACATACGGATCTAACATTACTGAAGCCA
AAATTAAACAAGCAAGCAATTTAAAATTTATCATGGTTTTTTCTGCGGGAGTAGATAGTTTGCCGAGGGAAATAATTCAA
GAAAGAAAAATTAAAGTTGCTAATGTACGTGGAATCCACGCTGTGCCAATGGGGGAATACGCGCTATCTTTCATGTTATC
ACACGTAAAAAAAGCCACTTTCTTCTATCGAATGCAAAAAGAAAGAAATTGGGCAAGCGAAGAACCAATTACAGAATTAG
CTGGAAAAACTTTGGTAGTAGCTGGAACTGGAGCCATTGGAGCAAAAGTGGCCGAGTTCGCTCAAGCATTTGATATGGAA
ATTATAGGTGTTAATACAACTGGACATCCTGTGAAGCCCTTTAGTAAAACATATGCCATGTCCGATATCGAAAAAGTGGC
TCCATTAGCAGATTTTTTTGTTAGTGTTTTACCTCATACAGACAAAACAACAGCTATTTATTCCTTATCATTTTTCCGGA
AAATGAAAGATAATGCAGTTTTTATCAATATAGGAAGAGGTAGCGCAGTTGAATTAAAGGTTTTAGAACAAGCATCAAAA
GAGCAATTAATAAACCATTTTTATTTAGATGTTGTACCTGAAGAGCCACTTTCCGAAGACAATTATTTATGGGAAGCAAG
TAACGTTACTATCACACCTCATGTATCTGGACATTCGGATAAATATTTAGAACGTAGCTTTAAGATTTGGTTTGAAAATA
TCAAACATCTAAAAGAAAATACCAAATTACGGAATGAAATTGATTTGAATAAAGGTTATTAA

Upstream 100 bases:

>100_bases
AAAAAATAAGATGCCGCAATCGAAAAAACAATTTAATTGCCGTTAAATGAAAAAAAGCATAAAATTAGTATATAGCTAAA
GAGAGGAAGTGTGGCGCAAA

Downstream 100 bases:

>100_bases
GCTATAAATTCTGCCATTCTATTGACAGTTAAGCGTTATTTCCGGTATTATAAAGATAATAATTATTTTTGGAAGGGAGT
GCATGGCGGTGTCTAATGCA

Product: glycerate dehydrogenase

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNILFTLDVPEHLQTLQAEKFPEDTFYYESNDHFANLAEIDIIVTYGSNITEAKIKQASNLKFIMVFSAGVDSLPREIIQ
ERKIKVANVRGIHAVPMGEYALSFMLSHVKKATFFYRMQKERNWASEEPITELAGKTLVVAGTGAIGAKVAEFAQAFDME
IIGVNTTGHPVKPFSKTYAMSDIEKVAPLADFFVSVLPHTDKTTAIYSLSFFRKMKDNAVFINIGRGSAVELKVLEQASK
EQLINHFYLDVVPEEPLSEDNYLWEASNVTITPHVSGHSDKYLERSFKIWFENIKHLKENTKLRNEIDLNKGY

Sequences:

>Translated_313_residues
MNILFTLDVPEHLQTLQAEKFPEDTFYYESNDHFANLAEIDIIVTYGSNITEAKIKQASNLKFIMVFSAGVDSLPREIIQ
ERKIKVANVRGIHAVPMGEYALSFMLSHVKKATFFYRMQKERNWASEEPITELAGKTLVVAGTGAIGAKVAEFAQAFDME
IIGVNTTGHPVKPFSKTYAMSDIEKVAPLADFFVSVLPHTDKTTAIYSLSFFRKMKDNAVFINIGRGSAVELKVLEQASK
EQLINHFYLDVVPEEPLSEDNYLWEASNVTITPHVSGHSDKYLERSFKIWFENIKHLKENTKLRNEIDLNKGY
>Mature_313_residues
MNILFTLDVPEHLQTLQAEKFPEDTFYYESNDHFANLAEIDIIVTYGSNITEAKIKQASNLKFIMVFSAGVDSLPREIIQ
ERKIKVANVRGIHAVPMGEYALSFMLSHVKKATFFYRMQKERNWASEEPITELAGKTLVVAGTGAIGAKVAEFAQAFDME
IIGVNTTGHPVKPFSKTYAMSDIEKVAPLADFFVSVLPHTDKTTAIYSLSFFRKMKDNAVFINIGRGSAVELKVLEQASK
EQLINHFYLDVVPEEPLSEDNYLWEASNVTITPHVSGHSDKYLERSFKIWFENIKHLKENTKLRNEIDLNKGY

Specific function: Unknown

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=236, Percent_Identity=29.2372881355932, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI23308577, Length=275, Percent_Identity=25.0909090909091, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4557497, Length=253, Percent_Identity=25.6916996047431, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI61743967, Length=253, Percent_Identity=25.6916996047431, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87082289, Length=255, Percent_Identity=28.6274509803922, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87081824, Length=269, Percent_Identity=23.7918215613383, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=269, Percent_Identity=27.1375464684015, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322116, Length=281, Percent_Identity=25.2669039145907, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320925, Length=254, Percent_Identity=24.4094488188976, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28574286, Length=243, Percent_Identity=24.6913580246914, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI45552429, Length=242, Percent_Identity=25.6198347107438, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24585514, Length=242, Percent_Identity=25.6198347107438, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28574282, Length=242, Percent_Identity=25.6198347107438, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28574284, Length=242, Percent_Identity=25.6198347107438, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI45551003, Length=242, Percent_Identity=25.6198347107438, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28571528, Length=192, Percent_Identity=28.125, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19921140, Length=269, Percent_Identity=23.7918215613383, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24585516, Length=231, Percent_Identity=25.1082251082251, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 35494; Mature: 35494

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILFTLDVPEHLQTLQAEKFPEDTFYYESNDHFANLAEIDIIVTYGSNITEAKIKQASN
CCEEEEECCHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEEEEECCCCCHHHHHHCCC
LKFIMVFSAGVDSLPREIIQERKIKVANVRGIHAVPMGEYALSFMLSHVKKATFFYRMQK
EEEEEEEECCCCHHHHHHHHHCCEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
ERNWASEEPITELAGKTLVVAGTGAIGAKVAEFAQAFDMEIIGVNTTGHPVKPFSKTYAM
HCCCCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHH
SDIEKVAPLADFFVSVLPHTDKTTAIYSLSFFRKMKDNAVFINIGRGSAVELKVLEQASK
HHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHH
EQLINHFYLDVVPEEPLSEDNYLWEASNVTITPHVSGHSDKYLERSFKIWFENIKHLKEN
HHHHHHHEEEECCCCCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHC
TKLRNEIDLNKGY
CCHHHCCCCCCCC
>Mature Secondary Structure
MNILFTLDVPEHLQTLQAEKFPEDTFYYESNDHFANLAEIDIIVTYGSNITEAKIKQASN
CCEEEEECCHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEEEEECCCCCHHHHHHCCC
LKFIMVFSAGVDSLPREIIQERKIKVANVRGIHAVPMGEYALSFMLSHVKKATFFYRMQK
EEEEEEEECCCCHHHHHHHHHCCEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
ERNWASEEPITELAGKTLVVAGTGAIGAKVAEFAQAFDMEIIGVNTTGHPVKPFSKTYAM
HCCCCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHH
SDIEKVAPLADFFVSVLPHTDKTTAIYSLSFFRKMKDNAVFINIGRGSAVELKVLEQASK
HHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHH
EQLINHFYLDVVPEEPLSEDNYLWEASNVTITPHVSGHSDKYLERSFKIWFENIKHLKEN
HHHHHHHEEEECCCCCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHC
TKLRNEIDLNKGY
CCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]