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The map label for this gene is metB [H]

Identifier: 226224283

GI number: 226224283

Start: 1722341

End: 1723465

Strand: Reverse

Name: metB [H]

Synonym: Lm4b_01693

Alternate gene names: 226224283

Gene position: 1723465-1722341 (Counterclockwise)

Preceding gene: 226224284

Following gene: 226224282

Centisome position: 59.17

GC content: 43.2

Gene sequence:

>1125_bases
ATGGCAAAGCTGAAACAAGAGACGATAGCAGCGCAAATTGGAAATAGAAAATGTGAAAGAACGGGTGCTGTCAATATGCC
AGTCTACTTCTCTACAGCTTACCAGCATGCCGATCTTGGAGTGTCAACGGGGTATGATTATACTAGAACTGGAAATCCGA
CGCGAGATGCTTTACAGGAAGCGCTCGCGGAACTAGAAAATGGAACGCATGCTTTTGCAACGAGTTCAGGGATGAGTGCG
ATTCAACTTGTTTTCCAGCTTTTTAAAACTGGCGAACATATTATTAGTTCACAGGATTTGTACGGGGGGACTTTTAGATA
TTTTGAACAGTTTGGTGCGCAATATAATATTGGATTTTCGTATTGGGACGGGGCGGAGATTGCTGATTTGGAGAAATTAG
TTCGTCCGGAAACGAAGGCGATTTTTATTGAGACACCAACAAATCCTTTAATGCAGGAAACAGATATTGCGGCTGTGTCT
GAATGGGCAGGTGCGCATGATTTACTTGTGATTGTGGATAATACATTTTATACACCAATTTTACAACAGCCGTTAACTTT
GGGCGCGGATATTGTGATTCATAGTGCGACGAAGTATCTTGGTGGGCATAATGATGTGCTTGCTGGGGCGGTTATTGTAA
AAGAGGAGCGACTTGGGAATTTCTTTTTTAATCAGTTAAATGCGACGGGTGCAGTGCTATCGCCATTTGATAGTTGGTTA
TTGATTCGTGGCCTTAAGACGCTGGTTTTGCGTGTAAGACAACATCAAGCGAATGCAGAGAAAATCGCGGCGTTTTTGGA
ATCACATGAATTGGTGGAGGAAGTTCGTTATCCAGGGCGCGGTGGGATGATTAGTTTCTTTATTCAAGATGCGGCGCTCG
TATCGCCACTTTTGAAAGAACTTGAGTTATTTACTTTTGCGGAAAGTTTAGGCGGGGTGGAGAGTTTGATTACTTATCCA
ACAACACAAACGCACGCAGATATTCCTGAAGAGTTGCGGAATTCTTATGGACTTACAGACAAATTATTGCGCATTTCGGT
TGGAATTGAAGCTAGTGAAGATTTGATTGCTGATTTATCTAAGGCACTCGATGCGGTTTTGGAAGGGGTGAGCGCTCGTG
GCTAA

Upstream 100 bases:

>100_bases
TGAAACGATTGCGGCACTTCAAGATATGATTAAAGCGACTAAAGAAGTTCGTGCGGAATATCAAGTATTAGAGAAATAAA
GGAATGGAGGAGAGGTAATT

Downstream 100 bases:

>100_bases
TGAATATGCGCAAGATACGGCTGTGATTAAAGCGAGCCTCGGGATTGATAAAGAGACAGGTGCGCTTAATACGCCAATTC
ATTTATCGTCTACTTTCCAT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-acetylhomoserine (thiol)-lyase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MAKLKQETIAAQIGNRKCERTGAVNMPVYFSTAYQHADLGVSTGYDYTRTGNPTRDALQEALAELENGTHAFATSSGMSA
IQLVFQLFKTGEHIISSQDLYGGTFRYFEQFGAQYNIGFSYWDGAEIADLEKLVRPETKAIFIETPTNPLMQETDIAAVS
EWAGAHDLLVIVDNTFYTPILQQPLTLGADIVIHSATKYLGGHNDVLAGAVIVKEERLGNFFFNQLNATGAVLSPFDSWL
LIRGLKTLVLRVRQHQANAEKIAAFLESHELVEEVRYPGRGGMISFFIQDAALVSPLLKELELFTFAESLGGVESLITYP
TTQTHADIPEELRNSYGLTDKLLRISVGIEASEDLIADLSKALDAVLEGVSARG

Sequences:

>Translated_374_residues
MAKLKQETIAAQIGNRKCERTGAVNMPVYFSTAYQHADLGVSTGYDYTRTGNPTRDALQEALAELENGTHAFATSSGMSA
IQLVFQLFKTGEHIISSQDLYGGTFRYFEQFGAQYNIGFSYWDGAEIADLEKLVRPETKAIFIETPTNPLMQETDIAAVS
EWAGAHDLLVIVDNTFYTPILQQPLTLGADIVIHSATKYLGGHNDVLAGAVIVKEERLGNFFFNQLNATGAVLSPFDSWL
LIRGLKTLVLRVRQHQANAEKIAAFLESHELVEEVRYPGRGGMISFFIQDAALVSPLLKELELFTFAESLGGVESLITYP
TTQTHADIPEELRNSYGLTDKLLRISVGIEASEDLIADLSKALDAVLEGVSARG
>Mature_373_residues
AKLKQETIAAQIGNRKCERTGAVNMPVYFSTAYQHADLGVSTGYDYTRTGNPTRDALQEALAELENGTHAFATSSGMSAI
QLVFQLFKTGEHIISSQDLYGGTFRYFEQFGAQYNIGFSYWDGAEIADLEKLVRPETKAIFIETPTNPLMQETDIAAVSE
WAGAHDLLVIVDNTFYTPILQQPLTLGADIVIHSATKYLGGHNDVLAGAVIVKEERLGNFFFNQLNATGAVLSPFDSWLL
IRGLKTLVLRVRQHQANAEKIAAFLESHELVEEVRYPGRGGMISFFIQDAALVSPLLKELELFTFAESLGGVESLITYPT
TQTHADIPEELRNSYGLTDKLLRISVGIEASEDLIADLSKALDAVLEGVSARG

Specific function: Catalyzes the transformation of O-succinylhomoserine into cystathionine, as well as O-acetylhomoserine and sulfide into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=361, Percent_Identity=42.382271468144, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI299473759, Length=364, Percent_Identity=39.010989010989, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI25453487, Length=360, Percent_Identity=37.5, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=40.9921671018277, Blast_Score=277, Evalue=8e-76,
Organism=Escherichia coli, GI1789383, Length=395, Percent_Identity=27.3417721518987, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17560056, Length=364, Percent_Identity=44.2307692307692, Blast_Score=293, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI17538055, Length=365, Percent_Identity=44.1095890410959, Blast_Score=290, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=37.466307277628, Blast_Score=217, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=367, Percent_Identity=41.9618528610354, Blast_Score=272, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=33.3333333333333, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=423, Percent_Identity=30.9692671394799, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=282, Percent_Identity=32.2695035460993, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6321904, Length=383, Percent_Identity=28.4595300261097, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI19922554, Length=364, Percent_Identity=40.6593406593407, Blast_Score=284, Evalue=9e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48; =2.5.1.49 [H]

Molecular weight: Translated: 41032; Mature: 40901

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLKQETIAAQIGNRKCERTGAVNMPVYFSTAYQHADLGVSTGYDYTRTGNPTRDALQE
CCCHHHHHHHHHHCCCHHHHCCCCCCCEEEECHHHHCCCCCCCCCCCCCCCCCHHHHHHH
ALAELENGTHAFATSSGMSAIQLVFQLFKTGEHIISSQDLYGGTFRYFEQFGAQYNIGFS
HHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCEE
YWDGAEIADLEKLVRPETKAIFIETPTNPLMQETDIAAVSEWAGAHDLLVIVDNTFYTPI
ECCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
LQQPLTLGADIVIHSATKYLGGHNDVLAGAVIVKEERLGNFFFNQLNATGAVLSPFDSWL
HHCCHHCCCHHEEEHHHHHHCCCCCHHHHHEEEEHHHHHHHHHHHHCCCCCCCCHHHHHH
LIRGLKTLVLRVRQHQANAEKIAAFLESHELVEEVRYPGRGGMISFFIQDAALVSPLLKE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHH
LELFTFAESLGGVESLITYPTTQTHADIPEELRNSYGLTDKLLRISVGIEASEDLIADLS
HHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHEEEEECCCCHHHHHHHHH
KALDAVLEGVSARG
HHHHHHHHHHCCCC
>Mature Secondary Structure 
AKLKQETIAAQIGNRKCERTGAVNMPVYFSTAYQHADLGVSTGYDYTRTGNPTRDALQE
CCHHHHHHHHHHCCCHHHHCCCCCCCEEEECHHHHCCCCCCCCCCCCCCCCCHHHHHHH
ALAELENGTHAFATSSGMSAIQLVFQLFKTGEHIISSQDLYGGTFRYFEQFGAQYNIGFS
HHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCEE
YWDGAEIADLEKLVRPETKAIFIETPTNPLMQETDIAAVSEWAGAHDLLVIVDNTFYTPI
ECCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
LQQPLTLGADIVIHSATKYLGGHNDVLAGAVIVKEERLGNFFFNQLNATGAVLSPFDSWL
HHCCHHCCCHHEEEHHHHHHCCCCCHHHHHEEEEHHHHHHHHHHHHCCCCCCCCHHHHHH
LIRGLKTLVLRVRQHQANAEKIAAFLESHELVEEVRYPGRGGMISFFIQDAALVSPLLKE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHH
LELFTFAESLGGVESLITYPTTQTHADIPEELRNSYGLTDKLLRISVGIEASEDLIADLS
HHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHEEEEECCCCHHHHHHHHH
KALDAVLEGVSARG
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]