Click here to switch to the map view.

The map label for this gene is menB

Identifier: 226224276

GI number: 226224276

Start: 1713408

End: 1714226

Strand: Reverse

Name: menB

Synonym: Lm4b_01686

Alternate gene names: 226224276

Gene position: 1714226-1713408 (Counterclockwise)

Preceding gene: 226224277

Following gene: 226224275

Centisome position: 58.85

GC content: 39.8

Gene sequence:

>819_bases
ATGAGTTTTAATTGGCAAACAGAAAAAGTATATGAGGAAATCAAATATGAAAGTTACGAAGGAATCGCGAAAATTACGAT
TAATCGTCCAGAAGTACATAATGCATTTACACCAAAAACAGTAACAGAGATGATTGATGCATTTAACTTAGCGCGTGATA
ATTCGAATCTTGGCGTGATTATTTTAACAGGGGAAGGCGAAAAAGCTTTCTGTTCTGGCGGAGACCAAAAAGTTCGCGGT
CATGGTGGATATGTTGGCGATGACCAAATCCCACGTTTGAATGTTCTTGATTTACAACGTTTAATTCGTGTTATTCCAAA
GCCAGTTATTGCGATGGTTGCGGGCTGGTCCATTGGTGGAGGTAATGTTCTTCAATTGGTATGTGACTTAACAATTGCAG
CAGATAATGCAAAATTTGGTCAAACAGGACCGAATGTTGGTAGCTTTGATGCTGGTTATGGTTCTGGCTATTTAGCACGT
GTTATTGGACATAAGAAAGCGAAAGAAGTTTGGTTTATGTGCCGTCAATATACAGCGGATGAAGCGCTAGAAATGGGCTG
GATTAACACTGTTGTTCCAGTAGCAGATTTAGAAAAAGAAACAGTAGCTTGGGCAAAAGAAATGCTACAAAAAAGTCCAA
CTGCCTTGCGTTTCATCAAAGCAGCTTTCAATGCAGATACAGATGGTTTAGCGGGTATTCAGCAATTAGCTGGTGACGCA
ACACTTCTATATTACACAACCGATGAAGCCAAAGAAGGTCGCGATGCCTTTAAAGAAAAACGTGATCCAGACTTTGACCA
ATTTCCAAAATTCCCTTGA

Upstream 100 bases:

>100_bases
CAATTCAAGAAGCGGGGCATGCAGTTTATTTAGAACAACCAAACAGCTTTTCAAGTCAGCTTAATTACTGGCTGGAAGAT
ATTTTGAAGGAGGAAGAAAA

Downstream 100 bases:

>100_bases
TTTGAAGGTGTAAAAATGGACATGACAAACTGGCTTCAAAAACGAGTGCGGCTTTCTCCTAAAGAGACCGCACTCGTTTT
TGAAGGAAAAGAAGAAACAT

Product: naphthoate synthase

Products: NA

Alternate protein names: DHNA-CoA synthase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSFNWQTEKVYEEIKYESYEGIAKITINRPEVHNAFTPKTVTEMIDAFNLARDNSNLGVIILTGEGEKAFCSGGDQKVRG
HGGYVGDDQIPRLNVLDLQRLIRVIPKPVIAMVAGWSIGGGNVLQLVCDLTIAADNAKFGQTGPNVGSFDAGYGSGYLAR
VIGHKKAKEVWFMCRQYTADEALEMGWINTVVPVADLEKETVAWAKEMLQKSPTALRFIKAAFNADTDGLAGIQQLAGDA
TLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP

Sequences:

>Translated_272_residues
MSFNWQTEKVYEEIKYESYEGIAKITINRPEVHNAFTPKTVTEMIDAFNLARDNSNLGVIILTGEGEKAFCSGGDQKVRG
HGGYVGDDQIPRLNVLDLQRLIRVIPKPVIAMVAGWSIGGGNVLQLVCDLTIAADNAKFGQTGPNVGSFDAGYGSGYLAR
VIGHKKAKEVWFMCRQYTADEALEMGWINTVVPVADLEKETVAWAKEMLQKSPTALRFIKAAFNADTDGLAGIQQLAGDA
TLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP
>Mature_271_residues
SFNWQTEKVYEEIKYESYEGIAKITINRPEVHNAFTPKTVTEMIDAFNLARDNSNLGVIILTGEGEKAFCSGGDQKVRGH
GGYVGDDQIPRLNVLDLQRLIRVIPKPVIAMVAGWSIGGGNVLQLVCDLTIAADNAKFGQTGPNVGSFDAGYGSGYLARV
IGHKKAKEVWFMCRQYTADEALEMGWINTVVPVADLEKETVAWAKEMLQKSPTALRFIKAAFNADTDGLAGIQQLAGDAT
LLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP

Specific function: Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4- dihydroxy-2-naphthoyl-CoA (DHNA-CoA) [H]

COG id: COG0447

COG function: function code H; Dihydroxynaphthoic acid synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=243, Percent_Identity=30.0411522633745, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI31542718, Length=262, Percent_Identity=30.5343511450382, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI45643119, Length=213, Percent_Identity=27.6995305164319, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=213, Percent_Identity=27.6995305164319, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=213, Percent_Identity=27.6995305164319, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4502327, Length=264, Percent_Identity=29.9242424242424, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI70995211, Length=229, Percent_Identity=31.4410480349345, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=66.6666666666667, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1787659, Length=263, Percent_Identity=29.6577946768061, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=31.5175097276265, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI87082183, Length=266, Percent_Identity=29.3233082706767, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=29.7709923664122, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=265, Percent_Identity=30.9433962264151, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=31.3492063492063, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=28.8461538461538, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=205, Percent_Identity=26.8292682926829, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=184, Percent_Identity=30.9782608695652, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=219, Percent_Identity=28.7671232876712, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129971, Length=267, Percent_Identity=31.8352059925094, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653477, Length=267, Percent_Identity=31.8352059925094, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=27.6264591439689, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=24.4274809160305, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357171, Length=189, Percent_Identity=30.1587301587302, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376
- InterPro:   IPR010198 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.1.3.36 [H]

Molecular weight: Translated: 30024; Mature: 29893

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNWQTEKVYEEIKYESYEGIAKITINRPEVHNAFTPKTVTEMIDAFNLARDNSNLGVI
CCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
ILTGEGEKAFCSGGDQKVRGHGGYVGDDQIPRLNVLDLQRLIRVIPKPVIAMVAGWSIGG
EEECCCCCCEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCC
GNVLQLVCDLTIAADNAKFGQTGPNVGSFDAGYGSGYLARVIGHKKAKEVWFMCRQYTAD
CHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
EALEMGWINTVVPVADLEKETVAWAKEMLQKSPTALRFIKAAFNADTDGLAGIQQLAGDA
HHHHHCCHHHHCCHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCE
TLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP
EEEEEECCCHHHHHHHHHHCCCCCHHHCCCCC
>Mature Secondary Structure 
SFNWQTEKVYEEIKYESYEGIAKITINRPEVHNAFTPKTVTEMIDAFNLARDNSNLGVI
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
ILTGEGEKAFCSGGDQKVRGHGGYVGDDQIPRLNVLDLQRLIRVIPKPVIAMVAGWSIGG
EEECCCCCCEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCC
GNVLQLVCDLTIAADNAKFGQTGPNVGSFDAGYGSGYLARVIGHKKAKEVWFMCRQYTAD
CHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
EALEMGWINTVVPVADLEKETVAWAKEMLQKSPTALRFIKAAFNADTDGLAGIQQLAGDA
HHHHHCCHHHHCCHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCE
TLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP
EEEEEECCCHHHHHHHHHHCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377 [H]