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The map label for this gene is metK

Identifier: 226224267

GI number: 226224267

Start: 1701621

End: 1702820

Strand: Reverse

Name: metK

Synonym: Lm4b_01677

Alternate gene names: 226224267

Gene position: 1702820-1701621 (Counterclockwise)

Preceding gene: 226224269

Following gene: 226224266

Centisome position: 58.46

GC content: 41.5

Gene sequence:

>1200_bases
TTGGCTAAGAACCGTCATCTATTTACATCAGAATCGGTTTCTGATGGACATCCAGATAAAATTGCAGATCAAATATCTGA
TGCAATTTTAGATGCAATTATTTCAAAAGATCCGGACGCACGTGTCGCTTGTGAGACTACTGTGACAACGGGTTTAGTAT
TAGTAGCAGGTGAGATTACCACCTCTGTTTATGTGGATATTCCTAAAATCGTGCGTGATACAATTAAAGAAATCGGCTAT
ACACGTGCAAAATATGGTTTCGATGCAGAAACGTGTGCCGTTTTAACAGCGATTGATGAACAATCCCCAGATATTGCGCA
AGGAGTCGACGAAGCACTAGAATCAAGAAGTGGTAAGGAAATCGATGCGGCTATTGAAGCAATCGGAGCGGGAGACCAAG
GATTAATGTTCGGTTTTGCAACAGATGAAACAGAAGAATTAATGCCACTGCCAATCTTTTTAGCACATGGCTTAGCACGT
AAGTTAACAGAATTACGTAAAACAAATAAATTAGACTACTTGCGTCCCGATGCGAAAACACAAGTAACGGTGGAATACGA
TGAATTTAATCAACCAGTTCGGATTGATACAATTGTTGTTTCGACGCAACATCATCCGGACATTACGCAAGAGCAAATCG
CAAAAGACTTGCACACGTACCTTTTCCCAGAAGTGATTGATGCCTCTTTCTTAGATGAAGATACGAAATACTTTATTAAT
CCAACTGGTCGCTTTGTTATTGGTGGTCCTCTTGGCGATGCGGGCTTAACTGGGCGTAAAATTATCGTGGATACATACGG
TGGTTATGCGCGTCACGGCGGGGGAGCGTTCTCCGGTAAAGATCCGACCAAAGTTGACCGATCGGGCGCTTATGCTGCAA
GGTATGTAGCTAAAAATATCGTTGCTGCAGGACTTGCTAAAAAAGTAGAAGTACAAGTCGCGTATGCTATCGGAGTTGCT
CGTCCAGTTTCCATTTCGATTGACACATATGGAACAAGTGACTATTCAGAACAAGAATTAATTGATGGCGTTAATGAGCT
ATTTGATTTACGCCCAGCTGGGATAATTCATATGCTTGATTTGCGTCGCCCAATTTATCGTCAAACAGCTGCATTTGGTC
ATTTTGGCCGTAGCGATCTTGATTTACCTTGGGAAAGAACCGATAAAGCAGAAGCCTTAAAAAAATTAATCGTAAAATAA

Upstream 100 bases:

>100_bases
TATAATTATAGCCTTTCTCTATTCGTGCGCGTTTTGTGCAAAATAGAGAGAGGCTTTTTATATGAGACGTATTTGGAGAG
AATTGAAGGAGGAAAATAAA

Downstream 100 bases:

>100_bases
GCTTAACCGAACATTTCTTGTAAGGAATGTTCGGTTTTTTTCTTTCCTAAACAGTGCAAAGAAAGTCTAGTTATGATATG
ATAGACGTTGTGAAAAAAAG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAKNRHLFTSESVSDGHPDKIADQISDAILDAIISKDPDARVACETTVTTGLVLVAGEITTSVYVDIPKIVRDTIKEIGY
TRAKYGFDAETCAVLTAIDEQSPDIAQGVDEALESRSGKEIDAAIEAIGAGDQGLMFGFATDETEELMPLPIFLAHGLAR
KLTELRKTNKLDYLRPDAKTQVTVEYDEFNQPVRIDTIVVSTQHHPDITQEQIAKDLHTYLFPEVIDASFLDEDTKYFIN
PTGRFVIGGPLGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSGAYAARYVAKNIVAAGLAKKVEVQVAYAIGVA
RPVSISIDTYGTSDYSEQELIDGVNELFDLRPAGIIHMLDLRRPIYRQTAAFGHFGRSDLDLPWERTDKAEALKKLIVK

Sequences:

>Translated_399_residues
MAKNRHLFTSESVSDGHPDKIADQISDAILDAIISKDPDARVACETTVTTGLVLVAGEITTSVYVDIPKIVRDTIKEIGY
TRAKYGFDAETCAVLTAIDEQSPDIAQGVDEALESRSGKEIDAAIEAIGAGDQGLMFGFATDETEELMPLPIFLAHGLAR
KLTELRKTNKLDYLRPDAKTQVTVEYDEFNQPVRIDTIVVSTQHHPDITQEQIAKDLHTYLFPEVIDASFLDEDTKYFIN
PTGRFVIGGPLGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSGAYAARYVAKNIVAAGLAKKVEVQVAYAIGVA
RPVSISIDTYGTSDYSEQELIDGVNELFDLRPAGIIHMLDLRRPIYRQTAAFGHFGRSDLDLPWERTDKAEALKKLIVK
>Mature_398_residues
AKNRHLFTSESVSDGHPDKIADQISDAILDAIISKDPDARVACETTVTTGLVLVAGEITTSVYVDIPKIVRDTIKEIGYT
RAKYGFDAETCAVLTAIDEQSPDIAQGVDEALESRSGKEIDAAIEAIGAGDQGLMFGFATDETEELMPLPIFLAHGLARK
LTELRKTNKLDYLRPDAKTQVTVEYDEFNQPVRIDTIVVSTQHHPDITQEQIAKDLHTYLFPEVIDASFLDEDTKYFINP
TGRFVIGGPLGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSGAYAARYVAKNIVAAGLAKKVEVQVAYAIGVAR
PVSISIDTYGTSDYSEQELIDGVNELFDLRPAGIIHMLDLRRPIYRQTAAFGHFGRSDLDLPWERTDKAEALKKLIVK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=388, Percent_Identity=59.2783505154639, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=59.1623036649215, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1789311, Length=392, Percent_Identity=61.734693877551, Blast_Score=487, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI25145633, Length=402, Percent_Identity=55.4726368159204, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=55.5269922879177, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=403, Percent_Identity=54.0942928039702, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=54.1450777202073, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=403, Percent_Identity=49.8759305210918, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25145635, Length=351, Percent_Identity=53.5612535612536, Blast_Score=357, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=54.1420118343195, Blast_Score=357, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=325, Percent_Identity=53.5384615384615, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=54.0229885057471, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=65.9090909090909, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=58.22454308094, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320710, Length=395, Percent_Identity=55.1898734177215, Blast_Score=416, Evalue=1e-117,
Organism=Drosophila melanogaster, GI45552159, Length=389, Percent_Identity=55.7840616966581, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580529, Length=389, Percent_Identity=55.7840616966581, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580537, Length=389, Percent_Identity=55.7840616966581, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580533, Length=389, Percent_Identity=55.7840616966581, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580535, Length=389, Percent_Identity=55.7840616966581, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580531, Length=389, Percent_Identity=55.7840616966581, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=55.012853470437, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=55.012853470437, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580541, Length=377, Percent_Identity=54.1114058355438, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=52.755905511811, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43624; Mature: 43493

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNRHLFTSESVSDGHPDKIADQISDAILDAIISKDPDARVACETTVTTGLVLVAGEIT
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCEEEEEECCE
TSVYVDIPKIVRDTIKEIGYTRAKYGFDAETCAVLTAIDEQSPDIAQGVDEALESRSGKE
EEEEEECHHHHHHHHHHCCCHHHHCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCH
IDAAIEAIGAGDQGLMFGFATDETEELMPLPIFLAHGLARKLTELRKTNKLDYLRPDAKT
HHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
QVTVEYDEFNQPVRIDTIVVSTQHHPDITQEQIAKDLHTYLFPEVIDASFLDEDTKYFIN
EEEEEHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
PTGRFVIGGPLGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSGAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VAAGLAKKVEVQVAYAIGVARPVSISIDTYGTSDYSEQELIDGVNELFDLRPAGIIHMLD
HHHHHHHHEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHH
LRRPIYRQTAAFGHFGRSDLDLPWERTDKAEALKKLIVK
HCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKNRHLFTSESVSDGHPDKIADQISDAILDAIISKDPDARVACETTVTTGLVLVAGEIT
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCEEEEEECCE
TSVYVDIPKIVRDTIKEIGYTRAKYGFDAETCAVLTAIDEQSPDIAQGVDEALESRSGKE
EEEEEECHHHHHHHHHHCCCHHHHCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCH
IDAAIEAIGAGDQGLMFGFATDETEELMPLPIFLAHGLARKLTELRKTNKLDYLRPDAKT
HHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
QVTVEYDEFNQPVRIDTIVVSTQHHPDITQEQIAKDLHTYLFPEVIDASFLDEDTKYFIN
EEEEEHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
PTGRFVIGGPLGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSGAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VAAGLAKKVEVQVAYAIGVARPVSISIDTYGTSDYSEQELIDGVNELFDLRPAGIIHMLD
HHHHHHHHEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHH
LRRPIYRQTAAFGHFGRSDLDLPWERTDKAEALKKLIVK
HCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]