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The map label for this gene is ansB

Identifier: 226224266

GI number: 226224266

Start: 1699619

End: 1701484

Strand: Reverse

Name: ansB

Synonym: Lm4b_01676

Alternate gene names: 226224266

Gene position: 1701484-1699619 (Counterclockwise)

Preceding gene: 226224267

Following gene: 226224263

Centisome position: 58.42

GC content: 37.89

Gene sequence:

>1866_bases
ATGTGTGGATTTGTAGGATGCGTACATGACCGCATTACAGAAATTACTGGCGCTGATAAAGAAACCTTTAGACAAATGAA
CAGTATGATTACGCACCGTGGGCCAGATGATGAAGGATACTTCACAGATGATCACGTGCAGTTTGGTTTCCGCCGTTTAA
GTATTATTGATGTAGAAAACGGTCATCAACCGTTAACGTATGAAAATGAACGTTACTGGATTATTTTTAATGGAGAAGTT
TATAACTATGTGGAACTCCGTGAGCAATTAGTTGCTGAAGGAATGACATTTGAAACCGAAAGTGATACCGAAGTAATTAT
CGCTACATATGCGAAATACAAAGAAAAAACAGCAGAACGTCTTCGTGGGATGTTTGGTTTCGTTATTTGGGATAAACAAG
AAGAACTTGTTTACGGCGCACGCGACCCATTTGGCATTAAACCATTTTTCTATGCAGAAGAAGATGGCAAATTGTTCATG
GGTTCTGAAAAGAAATCCATTCTACATGCTTTAAAAGAAAAAGAATTGGATGAAGTTTCATTACAAAACTATATGACTTA
CCAATTCGTTCCAGAACCAGATTCCTTAACTAAAAATGTAAAACGTTTAGAACCAGGACATCAGTTCACGAAGAAAATTG
GTCAACCAATGGAAATTACTACTTATTGGAAAGCATCTTTTGCACCAGTAACTAAATCAGAAGACGCATGGATTAAAGAA
GTGCGTGACGTGATGTATGATTCTGTAAAAATGCATATGCGCTCTGATGTACCAGTTGGTTCATTCCTATCTGGTGGTAT
TGATTCATCGATTATTGCTGCGATTGCTAAAGAATATCATCCTGCAATCAAAACATTCTCTGTTGGTTTTGAACGCGATG
GTTTCAGTGAAATCGATGTCGCAAAAGAAACAGCAGACAAACTTGGCGTAGAAAACATTAGTTATGTTATCTCACCTGAA
GAATATATGAAAGAATTACCAAAAATTGTTTGGCATATGGATGATCCACTTGCTGATCCAGCTGCTATTCCGCTTTACTT
CTTATCAAGAGAAGCGCGCAAACAAGTAACGGTTGCATTGTCCGGTGAAGGTGCAGATGAACTTTTCGGTGGTTATAACA
TTTATAACGAACCGAATTCCCTTGCGATGTTTAACAAAATGCCAAGCGTATTTAAATCAATGCTAAATAGCGTGGCGCGC
ATTATGCCAGAAGGTATGCGTGGTAAAAGTTTCTTAGAACGTGGAACCACTCCGATGGAAGAACGCTATATCGGAAACGC
CAAAATGTTCACGGAAAAAGAAAAACAAATTTTACTTCCAAAATATCAAGCTGGTCATGACTATACGAACATTACAGATC
CATTTTATGCAGAAACAACGAATTATCATCCTGTAGAAAGAATGCAGTACATTGATATTCATACGTGGCTTCGCGGAGAT
ATTCTTCTCAAAGCTGACCGCATGACGATGGCTAATTCGCTGGAAGTCCGTGTACCTTTCCTTGATAAAGAAGTGTACAA
TGTGGCAAGAAATATTCCAGATACAATGAAAACAACCAATGGAACAACCAAATATATCTTACGTAAAGCGGCAGCAACAT
TTGTACCAGAACACGTACTTAACCGCCGTAAACTAGGATTCCCAGTACCAATTCGTCACTGGTTAAAAGACGAAATGAAC
GCTTGGGTTAAAAACATCATTAAAGAATCACCAACAGATCATTTAATTAACAAACAATACGTTCTAGGATTACTAGACGA
TCACTGTGCCGGTAAATTCGACTACAGTCGTAAAATCTGGACAGTTGTTATTTTCATGATTTGGTATGATGTATACGTAG
CAGATAAATACGATTTTGGAAAATAA

Upstream 100 bases:

>100_bases
TTTTTCTTTCCTAAACAGTGCAAAGAAAGTCTAGTTATGATATGATAGACGTTGTGAAAAAAAGATAAAATGATTATCTA
TATAGAGGAGAAATGTTACC

Downstream 100 bases:

>100_bases
AAGAAAACCTAGACAAAGCTATTAATTAGCCTGTCTAGGTTTTCTTTTTTCGATGATAATGACGAATGGGGCATCATTTT
TTTGATTAATAAATTGATAA

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MCGFVGCVHDRITEITGADKETFRQMNSMITHRGPDDEGYFTDDHVQFGFRRLSIIDVENGHQPLTYENERYWIIFNGEV
YNYVELREQLVAEGMTFETESDTEVIIATYAKYKEKTAERLRGMFGFVIWDKQEELVYGARDPFGIKPFFYAEEDGKLFM
GSEKKSILHALKEKELDEVSLQNYMTYQFVPEPDSLTKNVKRLEPGHQFTKKIGQPMEITTYWKASFAPVTKSEDAWIKE
VRDVMYDSVKMHMRSDVPVGSFLSGGIDSSIIAAIAKEYHPAIKTFSVGFERDGFSEIDVAKETADKLGVENISYVISPE
EYMKELPKIVWHMDDPLADPAAIPLYFLSREARKQVTVALSGEGADELFGGYNIYNEPNSLAMFNKMPSVFKSMLNSVAR
IMPEGMRGKSFLERGTTPMEERYIGNAKMFTEKEKQILLPKYQAGHDYTNITDPFYAETTNYHPVERMQYIDIHTWLRGD
ILLKADRMTMANSLEVRVPFLDKEVYNVARNIPDTMKTTNGTTKYILRKAAATFVPEHVLNRRKLGFPVPIRHWLKDEMN
AWVKNIIKESPTDHLINKQYVLGLLDDHCAGKFDYSRKIWTVVIFMIWYDVYVADKYDFGK

Sequences:

>Translated_621_residues
MCGFVGCVHDRITEITGADKETFRQMNSMITHRGPDDEGYFTDDHVQFGFRRLSIIDVENGHQPLTYENERYWIIFNGEV
YNYVELREQLVAEGMTFETESDTEVIIATYAKYKEKTAERLRGMFGFVIWDKQEELVYGARDPFGIKPFFYAEEDGKLFM
GSEKKSILHALKEKELDEVSLQNYMTYQFVPEPDSLTKNVKRLEPGHQFTKKIGQPMEITTYWKASFAPVTKSEDAWIKE
VRDVMYDSVKMHMRSDVPVGSFLSGGIDSSIIAAIAKEYHPAIKTFSVGFERDGFSEIDVAKETADKLGVENISYVISPE
EYMKELPKIVWHMDDPLADPAAIPLYFLSREARKQVTVALSGEGADELFGGYNIYNEPNSLAMFNKMPSVFKSMLNSVAR
IMPEGMRGKSFLERGTTPMEERYIGNAKMFTEKEKQILLPKYQAGHDYTNITDPFYAETTNYHPVERMQYIDIHTWLRGD
ILLKADRMTMANSLEVRVPFLDKEVYNVARNIPDTMKTTNGTTKYILRKAAATFVPEHVLNRRKLGFPVPIRHWLKDEMN
AWVKNIIKESPTDHLINKQYVLGLLDDHCAGKFDYSRKIWTVVIFMIWYDVYVADKYDFGK
>Mature_621_residues
MCGFVGCVHDRITEITGADKETFRQMNSMITHRGPDDEGYFTDDHVQFGFRRLSIIDVENGHQPLTYENERYWIIFNGEV
YNYVELREQLVAEGMTFETESDTEVIIATYAKYKEKTAERLRGMFGFVIWDKQEELVYGARDPFGIKPFFYAEEDGKLFM
GSEKKSILHALKEKELDEVSLQNYMTYQFVPEPDSLTKNVKRLEPGHQFTKKIGQPMEITTYWKASFAPVTKSEDAWIKE
VRDVMYDSVKMHMRSDVPVGSFLSGGIDSSIIAAIAKEYHPAIKTFSVGFERDGFSEIDVAKETADKLGVENISYVISPE
EYMKELPKIVWHMDDPLADPAAIPLYFLSREARKQVTVALSGEGADELFGGYNIYNEPNSLAMFNKMPSVFKSMLNSVAR
IMPEGMRGKSFLERGTTPMEERYIGNAKMFTEKEKQILLPKYQAGHDYTNITDPFYAETTNYHPVERMQYIDIHTWLRGD
ILLKADRMTMANSLEVRVPFLDKEVYNVARNIPDTMKTTNGTTKYILRKAAATFVPEHVLNRRKLGFPVPIRHWLKDEMN
AWVKNIIKESPTDHLINKQYVLGLLDDHCAGKFDYSRKIWTVVIFMIWYDVYVADKYDFGK

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=422, Percent_Identity=28.6729857819905, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI168229252, Length=422, Percent_Identity=28.6729857819905, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI168229250, Length=422, Percent_Identity=28.6729857819905, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI296010848, Length=391, Percent_Identity=28.9002557544757, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI296010852, Length=317, Percent_Identity=27.7602523659306, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI296010850, Length=317, Percent_Identity=27.7602523659306, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI1786889, Length=401, Percent_Identity=30.4239401496259, Blast_Score=175, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI71993933, Length=401, Percent_Identity=31.1720698254364, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25147557, Length=401, Percent_Identity=31.1720698254364, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17560178, Length=409, Percent_Identity=29.8288508557457, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI25147560, Length=332, Percent_Identity=28.0120481927711, Blast_Score=115, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6325403, Length=487, Percent_Identity=27.3100616016427, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321563, Length=530, Percent_Identity=26.0377358490566, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45553209, Length=400, Percent_Identity=29.75, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71773; Mature: 71773

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFVGCVHDRITEITGADKETFRQMNSMITHRGPDDEGYFTDDHVQFGFRRLSIIDVEN
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCEEEEEEEEECC
GHQPLTYENERYWIIFNGEVYNYVELREQLVAEGMTFETESDTEVIIATYAKYKEKTAER
CCCCCEECCCEEEEEECCCCHHHHHHHHHHHHCCCCEECCCCCEEEEEHHHHHHHHHHHH
LRGMFGFVIWDKQEELVYGARDPFGIKPFFYAEEDGKLFMGSEKKSILHALKEKELDEVS
HHHHHEEEEECCCHHHEECCCCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHH
LQNYMTYQFVPEPDSLTKNVKRLEPGHQFTKKIGQPMEITTYWKASFAPVTKSEDAWIKE
HHHCEEEEEECCCHHHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHH
VRDVMYDSVKMHMRSDVPVGSFLSGGIDSSIIAAIAKEYHPAIKTFSVGFERDGFSEIDV
HHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHH
AKETADKLGVENISYVISPEEYMKELPKIVWHMDDPLADPAAIPLYFLSREARKQVTVAL
HHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEEEE
SGEGADELFGGYNIYNEPNSLAMFNKMPSVFKSMLNSVARIMPEGMRGKSFLERGTTPME
CCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHH
ERYIGNAKMFTEKEKQILLPKYQAGHDYTNITDPFYAETTNYHPVERMQYIDIHTWLRGD
HHHCCCCHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEHHHEECCC
ILLKADRMTMANSLEVRVPFLDKEVYNVARNIPDTMKTTNGTTKYILRKAAATFVPEHVL
EEEEECCHHHCCCCEEEECCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
NRRKLGFPVPIRHWLKDEMNAWVKNIIKESPTDHLINKQYVLGLLDDHCAGKFDYSRKIW
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHEEEHHCCCCCCCCCCHHHH
TVVIFMIWYDVYVADKYDFGK
HHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MCGFVGCVHDRITEITGADKETFRQMNSMITHRGPDDEGYFTDDHVQFGFRRLSIIDVEN
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCEEEEEEEEECC
GHQPLTYENERYWIIFNGEVYNYVELREQLVAEGMTFETESDTEVIIATYAKYKEKTAER
CCCCCEECCCEEEEEECCCCHHHHHHHHHHHHCCCCEECCCCCEEEEEHHHHHHHHHHHH
LRGMFGFVIWDKQEELVYGARDPFGIKPFFYAEEDGKLFMGSEKKSILHALKEKELDEVS
HHHHHEEEEECCCHHHEECCCCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHH
LQNYMTYQFVPEPDSLTKNVKRLEPGHQFTKKIGQPMEITTYWKASFAPVTKSEDAWIKE
HHHCEEEEEECCCHHHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHH
VRDVMYDSVKMHMRSDVPVGSFLSGGIDSSIIAAIAKEYHPAIKTFSVGFERDGFSEIDV
HHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHH
AKETADKLGVENISYVISPEEYMKELPKIVWHMDDPLADPAAIPLYFLSREARKQVTVAL
HHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEEEE
SGEGADELFGGYNIYNEPNSLAMFNKMPSVFKSMLNSVARIMPEGMRGKSFLERGTTPME
CCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHH
ERYIGNAKMFTEKEKQILLPKYQAGHDYTNITDPFYAETTNYHPVERMQYIDIHTWLRGD
HHHCCCCHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEHHHEECCC
ILLKADRMTMANSLEVRVPFLDKEVYNVARNIPDTMKTTNGTTKYILRKAAATFVPEHVL
EEEEECCHHHCCCCEEEECCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
NRRKLGFPVPIRHWLKDEMNAWVKNIIKESPTDHLINKQYVLGLLDDHCAGKFDYSRKIW
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHEEEHHCCCCCCCCCCHHHH
TVVIFMIWYDVYVADKYDFGK
HHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]