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The map label for this gene is trmB

Identifier: 226224216

GI number: 226224216

Start: 1641526

End: 1642170

Strand: Reverse

Name: trmB

Synonym: Lm4b_01626

Alternate gene names: 226224216

Gene position: 1642170-1641526 (Counterclockwise)

Preceding gene: 226224217

Following gene: 226224215

Centisome position: 56.38

GC content: 36.59

Gene sequence:

>645_bases
ATGCGTGTAAAACATAAACCATGGGCAAAAGATAGATTGGAAGAATTCCCAGCAATTTACATCAAAAACCCAGAAGATTT
CAAAGGTCAGTGGCGTGAAGTATTCGGAAATGATAATCCAGTCCATATCGAAATTGGTTCCGGTAAAGGTCAATTTATTA
GTGGCATGGCAAAAGCAAATCCGGAAATTAACTATATTGGTATCGAAATGATCGAAAGCGTGCTCGTTTCGGCGCTCGAT
AAAGCGATTGAAGCAGACGTTTCTAATTTACGATTAGTTGCTAGAGATGCTAAATTACTAGAAGAATGTTTTGAAAAAGG
AGAAATAGCACAAATTTATTTAAACTTCTCCGATCCATGGCCGAAAAAACGCCATACAAAACGTAGACTAACGAATCCAA
CGTTCTTAACTATTTATGAGCGACTGCTACCGGAAGCGGGGGAGATTCATTTCAAAACCGATAATCGCAGTTTGTTCGAA
TATTCGCTAGTAGCTTTCTCAGAATACAATATGTTATTAACTTTTGTATCTCTTGACCTGCATAATAGCGATTACGAAGG
GAATATTAAAACTGAATACGAAGAAAAATTTTCAGCTAAAGGATTCCCCATCTATCGTCTTGAAGCAAAATTTGATAGAA
ATTAA

Upstream 100 bases:

>100_bases
AACGGTTATGCAACTCATAACCGAGCATTCGAATGAAGCCAATGAACGAGCAAAACAAATTTTTCAAAATGCAATAGAAG
ATGATGAGGTGTAAAGAAAG

Downstream 100 bases:

>100_bases
CAAGGATAACCAGATGCATCTGCTGCATCTGGTTTTTATTTTTAATTGGAGGCGTTAAAAGTGGATTCCATTCAAATTGG
AAAAATAAAGATTTATTGGT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRVKHKPWAKDRLEEFPAIYIKNPEDFKGQWREVFGNDNPVHIEIGSGKGQFISGMAKANPEINYIGIEMIESVLVSALD
KAIEADVSNLRLVARDAKLLEECFEKGEIAQIYLNFSDPWPKKRHTKRRLTNPTFLTIYERLLPEAGEIHFKTDNRSLFE
YSLVAFSEYNMLLTFVSLDLHNSDYEGNIKTEYEEKFSAKGFPIYRLEAKFDRN

Sequences:

>Translated_214_residues
MRVKHKPWAKDRLEEFPAIYIKNPEDFKGQWREVFGNDNPVHIEIGSGKGQFISGMAKANPEINYIGIEMIESVLVSALD
KAIEADVSNLRLVARDAKLLEECFEKGEIAQIYLNFSDPWPKKRHTKRRLTNPTFLTIYERLLPEAGEIHFKTDNRSLFE
YSLVAFSEYNMLLTFVSLDLHNSDYEGNIKTEYEEKFSAKGFPIYRLEAKFDRN
>Mature_214_residues
MRVKHKPWAKDRLEEFPAIYIKNPEDFKGQWREVFGNDNPVHIEIGSGKGQFISGMAKANPEINYIGIEMIESVLVSALD
KAIEADVSNLRLVARDAKLLEECFEKGEIAQIYLNFSDPWPKKRHTKRRLTNPTFLTIYERLLPEAGEIHFKTDNRSLFE
YSLVAFSEYNMLLTFVSLDLHNSDYEGNIKTEYEEKFSAKGFPIYRLEAKFDRN

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=138, Percent_Identity=34.0579710144928, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 24904; Mature: 24904

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKHKPWAKDRLEEFPAIYIKNPEDFKGQWREVFGNDNPVHIEIGSGKGQFISGMAKAN
CCCCCCCCHHHHHHHCCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCC
PEINYIGIEMIESVLVSALDKAIEADVSNLRLVARDAKLLEECFEKGEIAQIYLNFSDPW
CCEEEEHHHHHHHHHHHHHHHHHHHCHHHHEEHHHHHHHHHHHHCCCCEEEEEEECCCCC
PKKRHTKRRLTNPTFLTIYERLLPEAGEIHFKTDNRSLFEYSLVAFSEYNMLLTFVSLDL
CCHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCEEHEEEHHHHHHCEEEEEEEEEE
HNSDYEGNIKTEYEEKFSAKGFPIYRLEAKFDRN
CCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MRVKHKPWAKDRLEEFPAIYIKNPEDFKGQWREVFGNDNPVHIEIGSGKGQFISGMAKAN
CCCCCCCCHHHHHHHCCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCC
PEINYIGIEMIESVLVSALDKAIEADVSNLRLVARDAKLLEECFEKGEIAQIYLNFSDPW
CCEEEEHHHHHHHHHHHHHHHHHHHCHHHHEEHHHHHHHHHHHHCCCCEEEEEEECCCCC
PKKRHTKRRLTNPTFLTIYERLLPEAGEIHFKTDNRSLFEYSLVAFSEYNMLLTFVSLDL
CCHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCEEHEEEHHHHHHCEEEEEEEEEE
HNSDYEGNIKTEYEEKFSAKGFPIYRLEAKFDRN
CCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]