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The map label for this gene is ykuU [H]

Identifier: 226224205

GI number: 226224205

Start: 1631528

End: 1632073

Strand: Reverse

Name: ykuU [H]

Synonym: Lm4b_01615

Alternate gene names: 226224205

Gene position: 1632073-1631528 (Counterclockwise)

Preceding gene: 226224206

Following gene: 226224203

Centisome position: 56.03

GC content: 40.66

Gene sequence:

>546_bases
GTGGCAGAACGTTTAGTAGGCACACAAGCTCCAAGATTTGAAATGGAAGCTGTTATGCCAAATCAGACTTTTGGGAAAGT
AAGTCTAGAAAAAAATATAGAAGACGATAAATGGACGATTCTCTTTTTCTATCCAATGGACTTTACATTTGTTTGTCCGA
CAGAAATTGTTGCTATTTCCGCTCGGTCAGATGAATTTGATGCATTAAATGCCCGCATAATTGGCGCTTCTACAGATACA
ATTCACTCGCATCTTGCATGGACGAATACACCGATTAAAGAAGGTGGTATTGGTAGATTAAACTACCCACTCGCTGCGGA
TACGAATCATCAAGTAGCTAGTGATTACGGTGTATTAATTGAGGAAGAGGGCGTAGCATTGCGCGGTCTTTTCATTATCA
ATCCAAAAGGTGAAATCCAGTACGAAGTGGTGCACCATAATAATATCGGGCGGGAAGTGGATGAAGTTTTAAGAGTTTTA
CAAGCTCTTCAAACAGGTGGACTGTGCCCAATCAACTGGCAACCCGGTGAAAAAACAATTGTTTAA

Upstream 100 bases:

>100_bases
TTTGTAATTAGTATAAATAAAGCTTTACAGTATTTCTTAGGTGTATTAAAATATATCATGTGATACAAAATACAACTACA
TAATTAATGGAGGGATAAAA

Downstream 100 bases:

>100_bases
ACTAAAAAACGCTTAGGAATTAATCCTAAGCGTCTTTTTTTTATACTAAGTTTTCTGGATTAAGTGCTTCTAATTCTGGA
AGGACGAAAATACCATCTTT

Product: 2-cys peroxiredoxin

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MAERLVGTQAPRFEMEAVMPNQTFGKVSLEKNIEDDKWTILFFYPMDFTFVCPTEIVAISARSDEFDALNARIIGASTDT
IHSHLAWTNTPIKEGGIGRLNYPLAADTNHQVASDYGVLIEEEGVALRGLFIINPKGEIQYEVVHHNNIGREVDEVLRVL
QALQTGGLCPINWQPGEKTIV

Sequences:

>Translated_181_residues
MAERLVGTQAPRFEMEAVMPNQTFGKVSLEKNIEDDKWTILFFYPMDFTFVCPTEIVAISARSDEFDALNARIIGASTDT
IHSHLAWTNTPIKEGGIGRLNYPLAADTNHQVASDYGVLIEEEGVALRGLFIINPKGEIQYEVVHHNNIGREVDEVLRVL
QALQTGGLCPINWQPGEKTIV
>Mature_180_residues
AERLVGTQAPRFEMEAVMPNQTFGKVSLEKNIEDDKWTILFFYPMDFTFVCPTEIVAISARSDEFDALNARIIGASTDTI
HSHLAWTNTPIKEGGIGRLNYPLAADTNHQVASDYGVLIEEEGVALRGLFIINPKGEIQYEVVHHNNIGREVDEVLRVLQ
ALQTGGLCPINWQPGEKTIV

Specific function: Directly Reduces Organic Hydroperoxides In Its Reduced Dithiol Form. [C]

COG id: COG0450

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Homo sapiens, GI4505591, Length=179, Percent_Identity=49.1620111731844, Blast_Score=186, Evalue=1e-47,
Organism=Homo sapiens, GI32455266, Length=179, Percent_Identity=49.1620111731844, Blast_Score=186, Evalue=1e-47,
Organism=Homo sapiens, GI32455264, Length=179, Percent_Identity=49.1620111731844, Blast_Score=186, Evalue=1e-47,
Organism=Homo sapiens, GI32189392, Length=179, Percent_Identity=48.0446927374302, Blast_Score=173, Evalue=9e-44,
Organism=Homo sapiens, GI32483377, Length=177, Percent_Identity=48.0225988700565, Blast_Score=170, Evalue=6e-43,
Organism=Homo sapiens, GI5802974, Length=179, Percent_Identity=47.4860335195531, Blast_Score=168, Evalue=2e-42,
Organism=Homo sapiens, GI5453549, Length=185, Percent_Identity=41.6216216216216, Blast_Score=160, Evalue=5e-40,
Organism=Homo sapiens, GI4758638, Length=190, Percent_Identity=31.5789473684211, Blast_Score=94, Evalue=6e-20,
Organism=Homo sapiens, GI33188454, Length=82, Percent_Identity=45.1219512195122, Blast_Score=78, Evalue=4e-15,
Organism=Escherichia coli, GI1786822, Length=182, Percent_Identity=32.967032967033, Blast_Score=108, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193204376, Length=184, Percent_Identity=49.4565217391304, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI32565831, Length=184, Percent_Identity=49.4565217391304, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17554494, Length=148, Percent_Identity=47.972972972973, Blast_Score=162, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI25153706, Length=190, Percent_Identity=26.3157894736842, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323613, Length=179, Percent_Identity=51.9553072625698, Blast_Score=179, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320661, Length=179, Percent_Identity=48.0446927374302, Blast_Score=167, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319407, Length=198, Percent_Identity=31.3131313131313, Blast_Score=108, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17157991, Length=176, Percent_Identity=48.8636363636364, Blast_Score=173, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24641739, Length=176, Percent_Identity=48.8636363636364, Blast_Score=173, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24656348, Length=181, Percent_Identity=45.8563535911602, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17864676, Length=181, Percent_Identity=45.8563535911602, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17738015, Length=179, Percent_Identity=45.8100558659218, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21357347, Length=177, Percent_Identity=44.6327683615819, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17975518, Length=190, Percent_Identity=33.1578947368421, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24652436, Length=190, Percent_Identity=33.1578947368421, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24652434, Length=190, Percent_Identity=33.1578947368421, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24581278, Length=186, Percent_Identity=30.6451612903226, Blast_Score=98, Evalue=4e-21,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2250 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 6040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1580 Molecules/Cell In: Stationary-Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000866
- InterPro:   IPR019479
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF10417 1-cysPrx_C; PF00578 AhpC-TSA [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 20168; Mature: 20037

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERLVGTQAPRFEMEAVMPNQTFGKVSLEKNIEDDKWTILFFYPMDFTFVCPTEIVAIS
CCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCEEEEEEECCCEEEECCCEEEEEE
ARSDEFDALNARIIGASTDTIHSHLAWTNTPIKEGGIGRLNYPLAADTNHQVASDYGVLI
CCCCCCCCCEEEEEECCCHHHHHHEEECCCCCCCCCCCEECCCEECCCCCCCCCCCCEEE
EEEGVALRGLFIINPKGEIQYEVVHHNNIGREVDEVLRVLQALQTGGLCPINWQPGEKTI
ECCCEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
V
C
>Mature Secondary Structure 
AERLVGTQAPRFEMEAVMPNQTFGKVSLEKNIEDDKWTILFFYPMDFTFVCPTEIVAIS
CCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCEEEEEEECCCEEEECCCEEEEEE
ARSDEFDALNARIIGASTDTIHSHLAWTNTPIKEGGIGRLNYPLAADTNHQVASDYGVLI
CCCCCCCCCEEEEEECCCHHHHHHEEECCCCCCCCCCCEECCCEECCCCCCCCCCCCEEE
EEEGVALRGLFIINPKGEIQYEVVHHNNIGREVDEVLRVLQALQTGGLCPINWQPGEKTI
ECCCEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]